This study determined the phytochemical composition and bioactivity(antioxidant and hypoglycemic)in five fractions(petroleum ether,chloroform,ethyl acetate,n-butanol,and water fractions)obtained from ethanol extract o...This study determined the phytochemical composition and bioactivity(antioxidant and hypoglycemic)in five fractions(petroleum ether,chloroform,ethyl acetate,n-butanol,and water fractions)obtained from ethanol extract of Amomum tsaoko Crevost et Lemaire(A.tsaoko).Qualitative and quantitative analyses of phenolic compounds were carried out with UHPLC-ESI-QTOF-MS/MS.50 bioactive compounds were identified from five different fractions,including 11 phenolic acids and derivatives,18 flavonoids and derivatives,14 proanthocyanidins,2 organic acids,and 5 other compounds.It was found that ethyl acetate fraction(EF)was enriched with the most types and contents of substances.Flavonoids and derivatives(including epicatechin and catechin)were the primary phenolic compounds in EF.In addition,EF demonstrated excellent antioxidant activity(DPPH and ABTS with IC_(50)=0.17 and 0.07 mg/mL,FRAP of 546.10 mg VCE/g DW)and anti-α-glucosidase activity(IC_(50)=20.14μg/mL).EF could ameliorate fasting blood glucose and glucose tolerance of diabetic mice.In conclusion,EF could be developed as a potential candidate for preventing or treating oxidative stress diseases and diabetes.展开更多
目的研究草果Amomum tsao-ko Crevost et Lemarie的化学成分。方法草果95%乙醇提取物经硅胶、聚酰胺柱、中压液相、高压制备液相等进行分离纯化,根据理化性质及波谱数据鉴定所得化合物的结构。结果从中分离得到10个化合物,分别鉴定为邻...目的研究草果Amomum tsao-ko Crevost et Lemarie的化学成分。方法草果95%乙醇提取物经硅胶、聚酰胺柱、中压液相、高压制备液相等进行分离纯化,根据理化性质及波谱数据鉴定所得化合物的结构。结果从中分离得到10个化合物,分别鉴定为邻苯二酚(1)、对羟基苯丙酸(2)、咖啡酸(3)、1-O-β-D-吡喃葡萄糖-2-羟基-4-烯丙基苯(4)、2′,3′-dihydroxyl-osthole(5)、(+)-loliolide(6)、1β,4β,7α-trihydroxyeudesmane(7)、R-5-(1R,5Sdimethyl-3R,4R,8S-trihydroxy-7-oxabicyclo[3,2,1]-oct-8-yl)-3-methyl-2Z,4E-pentadienoic acid(8)、tsaokoarylone(9)、7-bis-(4-hydroxyphenyl)-4E,6E-heptadien-3-one(10)。结论化合物2、4~8为首次从该植物中分离得到。展开更多
[目的]比较分析云南省不同产地草果(Amomum tsaoko Crevost et Lematre)叶中挥发油的化学成分,为其种属鉴定及进一步开发利用提供参考。[方法]采用气质联用技术研究了云南省4个不同产地(元阳小新乡、上新城乡,蒙自冷泉镇,文山州马关大...[目的]比较分析云南省不同产地草果(Amomum tsaoko Crevost et Lematre)叶中挥发油的化学成分,为其种属鉴定及进一步开发利用提供参考。[方法]采用气质联用技术研究了云南省4个不同产地(元阳小新乡、上新城乡,蒙自冷泉镇,文山州马关大篾厂乡)草果叶中的挥发油化学成分。从4种草果叶中共分离鉴定了61个化合物。其中9,12,15十-八碳三烯-1醇-的含量最高超过20%。其次,桉叶油醇和棕榈酸含量均超过10%。[结论]草果叶挥发油中组成丰富,可进一步进行开发利用。展开更多
基金The work was supported by Foundation of Key Laboratory of Tropical Crop Products Processing of Ministry of Agriculture and Rural Affairs,P.R.China(KFKT201901)Jiangxi Province Central Guidance Local Science and Technology Development Fund Project(20221ZDD02001 and 20212ZDD02008).
文摘This study determined the phytochemical composition and bioactivity(antioxidant and hypoglycemic)in five fractions(petroleum ether,chloroform,ethyl acetate,n-butanol,and water fractions)obtained from ethanol extract of Amomum tsaoko Crevost et Lemaire(A.tsaoko).Qualitative and quantitative analyses of phenolic compounds were carried out with UHPLC-ESI-QTOF-MS/MS.50 bioactive compounds were identified from five different fractions,including 11 phenolic acids and derivatives,18 flavonoids and derivatives,14 proanthocyanidins,2 organic acids,and 5 other compounds.It was found that ethyl acetate fraction(EF)was enriched with the most types and contents of substances.Flavonoids and derivatives(including epicatechin and catechin)were the primary phenolic compounds in EF.In addition,EF demonstrated excellent antioxidant activity(DPPH and ABTS with IC_(50)=0.17 and 0.07 mg/mL,FRAP of 546.10 mg VCE/g DW)and anti-α-glucosidase activity(IC_(50)=20.14μg/mL).EF could ameliorate fasting blood glucose and glucose tolerance of diabetic mice.In conclusion,EF could be developed as a potential candidate for preventing or treating oxidative stress diseases and diabetes.
文摘[目的]比较分析云南省不同产地草果(Amomum tsaoko Crevost et Lematre)叶中挥发油的化学成分,为其种属鉴定及进一步开发利用提供参考。[方法]采用气质联用技术研究了云南省4个不同产地(元阳小新乡、上新城乡,蒙自冷泉镇,文山州马关大篾厂乡)草果叶中的挥发油化学成分。从4种草果叶中共分离鉴定了61个化合物。其中9,12,15十-八碳三烯-1醇-的含量最高超过20%。其次,桉叶油醇和棕榈酸含量均超过10%。[结论]草果叶挥发油中组成丰富,可进一步进行开发利用。
