Introduction: European association of urology (EAU), American Urology Association (AUA) and French Association of Urology (AFU) recommend either Extracorporeal Lithotripsy or Ureteroscopy for the surgical management o...Introduction: European association of urology (EAU), American Urology Association (AUA) and French Association of Urology (AFU) recommend either Extracorporeal Lithotripsy or Ureteroscopy for the surgical management of stones smaller than 2 cm in size. The aim of this study was to analyse the management of upper urinary tract stones by ureteroscopy in the urology department of the Nord Franche Comte Hospital. Methods: This was a prospective, descriptive, single-centre study lasting seven (07) months from 02 November 2021 to 31 May 2022 and involving 71 patients treated for lithiasis of the upper urinary tract by ureteroscopy. The preoperative, preoperative and postoperative characteristics of the patients and the disease, including the radiological control results, were studied. Results: One hundred and four stones were treated, the mean age was 55.94 ± 17.22 years, the sex ratio M/F 1.84;the circumstances of discovery were renal colic in 73.2%. The mean size of the stones was 9.12 mm;mean density 951.45 HU;the left side was most affected in 54.93% of cases. The stone was unique in 69.01% of cases. The preoperative Cytobacterioligical urine exam (CBUE) was positive in 15.5% of patients. Préstinting was performed in 84.5% of patients. We performed 45.1% rigid Ureteroscopy and 39.4% flexible Ureteroscopy. The average duration of the procedure was 46.63 min. Monobloc stone extraction was 35.2%, laser fragmentation 31%. Drainage was not performed in 50.7% of patients. The outpatient and postoperative complication rates were 87.3% and 9.86%, respectively. The stone-free rate was 90.1%. Conclusion: This initial evaluation of Ureteroscopy at Nord Franche Comte Hospital (HNFC), although based on a small sample, is satisfactory and in line with the literature.展开更多
Caffeic acid-O-methyltransferase(COMT)is a crucial enzyme in the phenylpropanoid metabolic pathway,with significant roles in both the lignin and coumarin pathways.The function of COMT in plant disease resistance has b...Caffeic acid-O-methyltransferase(COMT)is a crucial enzyme in the phenylpropanoid metabolic pathway,with significant roles in both the lignin and coumarin pathways.The function of COMT in plant disease resistance has been demonstrated in several species.Our research identified the potato COMT gene family on a genome-wide scale and StCOMT1 as a candidate gene for enhancing potato disease resistance under DON induction through phylogenetic analyses combined with previously identified metabolic differences and weighted gene co-expression network analysis(WGCNA)results.In order to better understand the function of StCOMT1,heterologous expression and overexpression assays were conducted.StCOMT1 is localized in chloroplasts and was found to catalyze the methylation of substrates to produce ferulic acid and melatonin in vitro.Physiological parameters showed that,compared with wild-type potato plants,StCOMT1-overexpressing plants infected with Fusarium sporotrichioides exhibited smaller lesion areas and lower reactive oxygen species(ROS)levels.High-performance liquid chromatography(HPLC)and RT-qPCR analyses revealed organ-specific accumulation of coumarin-related compounds and organ-specific expression of their corresponding genes in StCOMT1-overexpressing plants post-inoculation.The results indicate that StCOMT1 overexpression in potatoes enhanced resistance to F.sporotrichioides by enhancing reactive oxygen species clearance and promoting organ-specific accumulation of coumarin-related compounds.展开更多
The cDNA fragment encoding caffeic acid 3_O_methyltransferase (COMT) in Chinese white poplar ( Populus tomentosa Carr.) was isolated and cloned by RT_PCR technique. The size of the cDNA fragment is 1 080 bp, which alm...The cDNA fragment encoding caffeic acid 3_O_methyltransferase (COMT) in Chinese white poplar ( Populus tomentosa Carr.) was isolated and cloned by RT_PCR technique. The size of the cDNA fragment is 1 080 bp, which almost covers the whole cDNA_encoding region. Authors’ cDNA fragment in P. tomentosa shares 98.7% homology with the reported corresponding cDNA in the P. tremuloids at nucleotide level, 99.4% homology at amino acid level, respectively. The analysis of Northern dot hybridization showed that COMT is expressed specifically in the developing secondary xylem of stem during the season of xylem differentiation, which means the linkage between the gene expression for a monolignol biosynthetic enzyme and seasonal regulation of xylem development in woody plant.展开更多
文摘Introduction: European association of urology (EAU), American Urology Association (AUA) and French Association of Urology (AFU) recommend either Extracorporeal Lithotripsy or Ureteroscopy for the surgical management of stones smaller than 2 cm in size. The aim of this study was to analyse the management of upper urinary tract stones by ureteroscopy in the urology department of the Nord Franche Comte Hospital. Methods: This was a prospective, descriptive, single-centre study lasting seven (07) months from 02 November 2021 to 31 May 2022 and involving 71 patients treated for lithiasis of the upper urinary tract by ureteroscopy. The preoperative, preoperative and postoperative characteristics of the patients and the disease, including the radiological control results, were studied. Results: One hundred and four stones were treated, the mean age was 55.94 ± 17.22 years, the sex ratio M/F 1.84;the circumstances of discovery were renal colic in 73.2%. The mean size of the stones was 9.12 mm;mean density 951.45 HU;the left side was most affected in 54.93% of cases. The stone was unique in 69.01% of cases. The preoperative Cytobacterioligical urine exam (CBUE) was positive in 15.5% of patients. Préstinting was performed in 84.5% of patients. We performed 45.1% rigid Ureteroscopy and 39.4% flexible Ureteroscopy. The average duration of the procedure was 46.63 min. Monobloc stone extraction was 35.2%, laser fragmentation 31%. Drainage was not performed in 50.7% of patients. The outpatient and postoperative complication rates were 87.3% and 9.86%, respectively. The stone-free rate was 90.1%. Conclusion: This initial evaluation of Ureteroscopy at Nord Franche Comte Hospital (HNFC), although based on a small sample, is satisfactory and in line with the literature.
基金supported by the National Natural Science Foundation of China(U22A20443)the Heilongjiang Provincial Research Institutes Scientific Research Operating Expenses Project,China(CZKYF2023-1-B020)+1 种基金the Key Research and Development Plan Project of Heilongjiang Province,China(GA23B015)the Young Scientists Fund of the National Natural Science Foundation of China(32201717)。
文摘Caffeic acid-O-methyltransferase(COMT)is a crucial enzyme in the phenylpropanoid metabolic pathway,with significant roles in both the lignin and coumarin pathways.The function of COMT in plant disease resistance has been demonstrated in several species.Our research identified the potato COMT gene family on a genome-wide scale and StCOMT1 as a candidate gene for enhancing potato disease resistance under DON induction through phylogenetic analyses combined with previously identified metabolic differences and weighted gene co-expression network analysis(WGCNA)results.In order to better understand the function of StCOMT1,heterologous expression and overexpression assays were conducted.StCOMT1 is localized in chloroplasts and was found to catalyze the methylation of substrates to produce ferulic acid and melatonin in vitro.Physiological parameters showed that,compared with wild-type potato plants,StCOMT1-overexpressing plants infected with Fusarium sporotrichioides exhibited smaller lesion areas and lower reactive oxygen species(ROS)levels.High-performance liquid chromatography(HPLC)and RT-qPCR analyses revealed organ-specific accumulation of coumarin-related compounds and organ-specific expression of their corresponding genes in StCOMT1-overexpressing plants post-inoculation.The results indicate that StCOMT1 overexpression in potatoes enhanced resistance to F.sporotrichioides by enhancing reactive oxygen species clearance and promoting organ-specific accumulation of coumarin-related compounds.
文摘The cDNA fragment encoding caffeic acid 3_O_methyltransferase (COMT) in Chinese white poplar ( Populus tomentosa Carr.) was isolated and cloned by RT_PCR technique. The size of the cDNA fragment is 1 080 bp, which almost covers the whole cDNA_encoding region. Authors’ cDNA fragment in P. tomentosa shares 98.7% homology with the reported corresponding cDNA in the P. tremuloids at nucleotide level, 99.4% homology at amino acid level, respectively. The analysis of Northern dot hybridization showed that COMT is expressed specifically in the developing secondary xylem of stem during the season of xylem differentiation, which means the linkage between the gene expression for a monolignol biosynthetic enzyme and seasonal regulation of xylem development in woody plant.
