摘要
[目的]鉴定辣椒COMT基因家族成员,探究其对辣椒盐胁迫的响应,为辣椒抗逆高产育种提供帮助。[方法]基于拟南芥COMT基因筛选辣椒COMT基因家族,并利用生物信息学方法对辣椒COMT基因家族进行基因染色体定位、重命名、蛋白质理化性质分析、构建系统进化树、保守基序、蛋白结构和基因结构的分析。[结果]从辣椒基因中鉴定出59个COMT基因家族成员,分别分布于10条染色体上,蛋白质分子质量差异较大,大多数属于稳定蛋白,除一条外均属于两性蛋白。与拟南芥构建进化树后发现辣椒COMT基因家族与拟南芥COMT基因家族亲缘关系较远,可将CaCOMT分为3个亚组,亚组内各成员拥有多数相同的保守基序,基因结构上外显子数量也接近,可见亚组内成员的亲缘关系较近。[结论]该研究为研究CaCOMT基因功能奠定了基础。
[Objective]In order to identify the members of the COMT gene family in Capsicum annuum and explore their response to salt stress,and provide help for high-yield breeding of C.annuum.[Method]The COMT gene family of C.annuum was screened based on the COMT gene of Arabidopsis thaliana,and the chromosome mapping,renaming,physical and chemical properties of proteins,phylogenetic tree construction,conserved motifs,protein structure and gene structure were analyzed by bioinformatics method.[Result]59 members of the COMT gene family were identified from the C.annuum gene,which were distributed on 10 chromosomes respectively.The molecular weight of the proteins was very different,most of them belonged to stable proteins,and all of them belonged to amphoteric proteins except one.After constructing an evolutionary tree with A.thaliana,it was found that the COMT gene family of C.annuum was distantly related to the COMT gene family of A.t haliana,and CaCOMT could be divided into three subgroups,in which members had most of the same conserved motifs and the number of exons in gene structure was similar,indicating that members in the subgroup were closely related.[Conclusion]This study laid a foundation for the research on the functions of the CaCOMT gene.
作者
林婉盈
廖万兰
王晓
乔英豪
刘生财
LIN Wan-ying(Institute of Horticultural Biotechnology,Fujian Agriculture and Forestry University,Fuzhou,Fujian350002)
出处
《园艺与种苗》
2025年第3期15-17,23,共4页
Horticulture & Seed
基金
福建农林大学大学生创新训练项目,福建农林大学社会服务团队。
