Human immortal keratinocyte cells(HaCaT)are induced with UVB to establish an injury model.This model is utilized to investigate whether oat bran fermentation broth(OBF)has a reparative effect on skin inflammation and ...Human immortal keratinocyte cells(HaCaT)are induced with UVB to establish an injury model.This model is utilized to investigate whether oat bran fermentation broth(OBF)has a reparative effect on skin inflammation and damage to the skin barrier caused by UVB irradiation.The results show that compared with unfermented oat bran(OB),OBF exhibits higher structural homogeneity,increased molecular weight size,active substances content,and in vitro antioxidant activity.OBF has a scavenging effect on excess reactive oxygen species(ROS)and increases the intracellular levels of antioxidant enzymes.It was found that OBF has a stronger inhibitory effect on the release of inflammatory factors than OB.It increases the synthesis of AQP3 and FLG proteins while decreasing the secretion of KLK-7.OBF can inhibit the transcription level of inflammatory factors by suppressing the JAK/STAT signaling pathway.Safety experiments demonstrate that OBF has a high safety profile.展开更多
This study aimed to explore the protective effect and potential mechanism of Nostoc commune Vauch.polysaccharide(NCVP)on lead(Pb)-poisoning mice.NCVP improved Pb-induced hepatorenal toxicity and inflammatory responses...This study aimed to explore the protective effect and potential mechanism of Nostoc commune Vauch.polysaccharide(NCVP)on lead(Pb)-poisoning mice.NCVP improved Pb-induced hepatorenal toxicity and inflammatory responses and modulated key indicators of antioxidant capacity.Moreover,the down-regulation of critical proteins of the Nrf2 pathway induced by Pb could be reversed after NCVP intervention.In addition,NCVP maintained the diversity of gut bacteriobiota and restored the relative abundance of f_Prevotellaceae,g_Alloprevotella,and f_Eubacterium_coprostanoligenes_group reduced by Pb.Also,NCVP regulated the diversity and abundance of gut mycobiota affected by Pb.Specifically,Pb decreased the proportion of pathogenic species(g_Fusarium,p_Basidiomycota,g_Alternaria,g_Aspergillus,and g_Candida)while NCVP increased the abundance of probiotics species(g_Kazachstania and p_Ascomycota).Furthermore,the metabolomic analysis found that NCVP significantly altered a range of microbial metabolites,including porphobilinogen,cromakalim,salidroside,and trichostatin A,which has significant associations with specific gut bacteriobiota or mycobiota.These altered metabolites are involved in primary bile acid biosynthesis,metabolism of xenobiotics by cytochrome P450,lysine degradation,and other metabolic pathways.Overall,our findings indicate that NCVP might be an excellent natural product for eliminating Pb-induced hepatorenal toxicity,possibly by regulating gut bacteriome,mycobiome and metabolome.展开更多
The objectives of this work were to evaluate the surgical activities carried out in the general surgery department of the Reference Health Center of Commune I of Bamako, to describe the sociodemographic characteristic...The objectives of this work were to evaluate the surgical activities carried out in the general surgery department of the Reference Health Center of Commune I of Bamako, to describe the sociodemographic characteristics of the operated patients, to determine the main pathologies encountered and to evaluate qualitatively the result of the treatment. In order to improve performance, and the quality of care, and to identify common pathologies in the surgical department, we undertook a retrospective study on surgical activities from January 2009 to December 2010. At the end of this study, out of 474 men and 187 women (equal sex ratio 2.53);we were able to determine the frequency of surgical pathologies. Farmers, housewives and pupils/students were the most represented with 25.9% respectively;20% and 13.3%. The most frequently observed pathologies were wall hernia (44.8%), prostate adenoma (12%) and acute appendicitis (10.5%). The average length of hospitalization was 3.43 days. Infectious complications affected 25 patients (3.8% of cases) and a death rate of 0.45% (i.e. 3 patients). The average cost of care was 53,500 FCFA. Indeed, the reality of surgical practice in health centers was not the same because of the level of skills of practicing surgeons.展开更多
Bioassay-guided fractionation of the crude extract from Penicillium commune SD-118, a fungus obtained from a deep-sea sediment sample, resulted in the isolation of a known antibacterial compound, xanthocillin X (1), a...Bioassay-guided fractionation of the crude extract from Penicillium commune SD-118, a fungus obtained from a deep-sea sediment sample, resulted in the isolation of a known antibacterial compound, xanthocillin X (1), and 14 other known compounds comprising three steroids (2-4), two ceramides (5 and 6), six aromatic compounds (7-12), and three alkaloids (13-15). Xanthocillin X (1) was isolated for the first time from a marine fungus. In the bioassay, xanthocillin X (1) displayed remarkable antimicrobial activity against Staphylococcus aureus and Escherichia coli, and significant cytotoxicity against MCF-7, HepG2, H460, Hela, Du145, and MDA-MB-231 cell lines. Meleagrin (15) exhibited cytotoxicity against HepG2, Hela, Du145, and MDA-MB-231 cell lines. This is the first report of the cytotoxicity of xanthocillin X (1).展开更多
The present study was designed to search for compounds with analgesic activity from the Schizophyllum commune(SC), which is widely consumed as edible and medicinal mushroom world. Thin layer chromatography(TLC), tosil...The present study was designed to search for compounds with analgesic activity from the Schizophyllum commune(SC), which is widely consumed as edible and medicinal mushroom world. Thin layer chromatography(TLC), tosilica gel column chromatography, sephadex LH 20, and reverse-phase high performance liquid chromatography(RP-HPLC) were used to isolate and purify compounds from SC. Structural analysis of the isolated compounds was based on nuclear magnetic resonance(NMR). The effects of these compounds on voltage-gated sodium(NaV) channels were evaluated using patch clamp. The analgesic activity of these compounds was tested in two types of mouse pain models induced by noxious chemicals. Five phenolic acids identified from SC extracts in the present study included vanillic acid, m-hydroxybenzoic acid, o-hydroxybenzeneacetic acid, 3-hydroxy-5-methybenzoic acid, and p-hydroxybenzoic acid. They inhibited the activity of both tetrodotoxin-resistant(TTX-r) and tetrodotoxin-sensitive(TTX-s) NaV channels. All the compounds showed low selectivity on NaV channel subtypes. After intraperitoneal injection, three compounds of these compounds exerted analgesic activity in mice. In conclusion, phenolic acids identified in SC demonstrated analgesic activity, facilitating the mechanistic studies of SC in the treatment of neurasthenia.展开更多
The fruits of Pyracantha fortuneana(Maxim.)Li were fermented with pure fungi to improve their antioxidant capacity.The antioxidant activities of fermented and non-fermented P.fortuneana fruits were estimated by 1,1-di...The fruits of Pyracantha fortuneana(Maxim.)Li were fermented with pure fungi to improve their antioxidant capacity.The antioxidant activities of fermented and non-fermented P.fortuneana fruits were estimated by 1,1-diphenyl-2-picrylhydrazyl(DPPH)and 2,2'-azinobis-(3-ethylbenzthiazoline-6-sulphonate)(ABTS)radical scavenging activities,as well as ferric ion reducing antioxidant power(FRAP).The total phenolic content(TPC)and total flavonoid content(TFC)were also determined.Additionally,the changes caused by fermentation were characterized by proton nuclear magnetic resonance spectroscopy(1H NMR).The results showed that P.fortuneana fruits fermented with Rhizopus oryzae and Penicillium commune exhibited stronger antioxidant activity compared with the non-fermented material.Correlation analysis indicated that the increased activity might be ascribed to the improvement of TPC.Chemical characterization of 1H NMR implied that the increased TPC and antioxidant activity might be attributed to some of the phenolic and flavonoid glycosides hydrolyzed to new components during fermentation.The present study was the first report of the fermentation of P.fortuneana fruit with pure fungal strains and suggested that fermentation of P.fortuneana fruits with R.oryzae and P.commune was an effective alternative approach to increase antioxidant,and P.fortuneana fruit fermented by R.oryzae and P.commune might be the new alternative of natural antioxidants.展开更多
The effects of La(NO3)(3) on the liquid culture and the contents of amino acids of Nostoc commune flagelliforme cells were studied. It is indicated from the experimental results that the growth of Nostoc commune flage...The effects of La(NO3)(3) on the liquid culture and the contents of amino acids of Nostoc commune flagelliforme cells were studied. It is indicated from the experimental results that the growth of Nostoc commune flagelliforme cells can be stimulated by La(NO3)(3) at the concentrations from 1 to 200 mg.L-1, and among them the treatment of 100 mg.L-1 La(NO3)(3) has the largest A(670) and dry weight which increased by 64.9% and 61.1%, respectively as compared with the control, and La(NO3)(3) can also influence the contents of some kinds of amino acids in Nostoc commune flagelliforme cells. The contents of aspartic acid and glutamic acid decreased by 59.9% and 57.9%, respectively, and that of proline increased by 24.5% with the treatment of 200 mg.L-1 La(NO3)(3).展开更多
Using Nostoc commune as experiment target,the effect of CO 2 with different concentrations in atmosphere and dosage of UV-B on photosynthetic pigment chlorophyll a （Chl a）,carotenoids,phycoerythrin,phycocyanin,allop...Using Nostoc commune as experiment target,the effect of CO 2 with different concentrations in atmosphere and dosage of UV-B on photosynthetic pigment chlorophyll a （Chl a）,carotenoids,phycoerythrin,phycocyanin,allophycocyanin and UV-B masking materials was studied.The results showed that high dosage of UV-B radiation inhibited the synthesis process of Chl a,phycoerythrin,phycocyanin and allophycocyanin,but promoted the synthesis of carotenoids,MAAs and scytonemin,thus the negative effect produced by UV-B radiation was reduced and the resistance of Nostoc commune against UV-B radiation was enhanced.展开更多
Phytochemical investigation of the endophytic fungus Penicillium commune led to the isolation of nine known compounds. Based on the spectroscopic and chemical properties, their structures were identified as 6-acetyl-2...Phytochemical investigation of the endophytic fungus Penicillium commune led to the isolation of nine known compounds. Based on the spectroscopic and chemical properties, their structures were identified as 6-acetyl-2α,5-dihydroxy-2-（2-hydroxypropyl）-3α,8-dimethylchroman（1）, communol B（2）, communol E（3）, communol G（4）, 2-hydroxy-4-phenylquinoline（5）, viridicatol（6）, 3-O-methylviridicatin（7）, trans-ferulic acid（8）, 1,2-benzenediol,4-（2-methoxyethenyl）（9）. Compounds 1 and 5–9 were isolated from this fungus for the first time.展开更多
The schizophyllan from Schizophyllum commune was purified and characterized. The crude schizo-phyllan was obtained from Schizophyllum commune fermentation broth by decoloration with activated carbon,followed by deprot...The schizophyllan from Schizophyllum commune was purified and characterized. The crude schizo-phyllan was obtained from Schizophyllum commune fermentation broth by decoloration with activated carbon,followed by deproteinization with Sevag method and ethanol precipitation. The pure schizophyllan was obtained by gel filtration chromatography with Sephacryl S-500,and its molecular characteristics were examined. The result showed that the molecular weight was 3.8×104 ,and the schizophyllan consisted of glucose with β-(1→6)-glucosidic linkages.展开更多
Artificial induction of labor (AIL) is the set of techniques intended to induce childbirth in order to reduce maternal-fetal mortality. The aim of this paper was to study the maternal and fetal prognosis after the art...Artificial induction of labor (AIL) is the set of techniques intended to induce childbirth in order to reduce maternal-fetal mortality. The aim of this paper was to study the maternal and fetal prognosis after the artificial induction of labor with misoprostol. It was a descriptive prospective study conducted from September 1, 2018 to February 28, 2019 at the maternity unit of the Referral Health Center (RHC) in commune I of Bamako. It was about any pregnant woman at term who had received misoprostol as part of the artificial induction of labor. 102 cases of artificial induction with misoprostol 200 μg were collected out of 3641 deliveries, or a frequency of 2.8%. The age group 28 - 32 years accounted for 56.86% with an average age of 28.8 years. Multiparous represented 54.90% of the number. The history of diabetes and hypertension involved 37.25% of the pregnant women. The main indication was premature rupture of membranes (PRM) (40.2%). Induction was performed between the 37</span><sup><span style="font-family:Verdana;">th</span></sup><span style="font-family:Verdana;"> and 41</span><sup><span style="font-family:Verdana;">st</span></sup><span style="font-family:Verdana;"> week of amenorrhea (69.6%). The trigger index according to BISHOP was greater than 7 (50.98%). Induction was done only with 3/4 of misoprostol tablet 200 μg (43.14%). The average labor time was 7 hours (89.22%). The vaginal birth concerned 92.16% of pregnant women. An APGAR10 was noted in 97.85% of newborns after 05 minutes. AIL with misoprostol was practiced at the Center. The main indication was the premature rupture of the membranes. It improves the maternal and fetal prognosis.展开更多
Land resource depletion causes a range of consequences,such as threats to food security,increasing poverty,and social unrest.Therefore,reasonable and effective land use has become a crucial issue of the nation and loc...Land resource depletion causes a range of consequences,such as threats to food security,increasing poverty,and social unrest.Therefore,reasonable and effective land use has become a crucial issue of the nation and localities nationwide.Research results in Tan Binh commune,Dak Doa district,Gia Lai Province in Vietnam showed that long-term industrial and agricultural land occupies most of the commune's agroforestry land.The study also had shown that there are six common land use types(LUTs)in the commune.In particular,the LUT of pepper cultivation had the highest economic benefit,whereas the LUT of coffee brought the highest social benefit.However,the LUT of chayote was the highest in overall benefits with Ect of 0.93;thus,this LUT should be widely applied throughout Tan Binh commune.The research results were a crucial scientific basis for proposing a group of suitable plants to local natural conditions to bring effective and sustainable efficiency in land-use types.展开更多
<strong>Background:</strong> Dengue is under-reported in the indicators and directories of health statistics in Togo and the diagnostic hypothesis of dengue during medical consultations is often not mentio...<strong>Background:</strong> Dengue is under-reported in the indicators and directories of health statistics in Togo and the diagnostic hypothesis of dengue during medical consultations is often not mentioned. <strong>Objective:</strong> The objective is to assess healthcare workers’ knowledge of dengue and its management in public health centers in the health district of Lomé Commune. <strong>Materials and Methods: </strong>This is a cross-sectional and descriptive study that took place from November 1, 2019 to February 29, 2020 in the health district of Lomé Commune. <strong>Results: </strong>This study involved 280 healthcare workers out of 310 concerned, <em>i.e.</em>, a participation rate of 90.32%, distributed over the 22 public health centers of the health district of Lomé Commune. The age of the healthcare workers ranged from 18 to 58 years with an average age of 33 years (±8.14). The age group [25 - 35 years] was more represented with 53.20%. Physicians were more represented with 46.07%. The majority of healthcare professionals were aware of the existence of dengue (90.36%). The essential clinical signs were more or less known, especially by the physicians. Dengue hemorrhagic fever was the most known clinical form and represented 70%. Treatment was symptomatic according to the majority (63.21%) and was based on analgesics (71.43%) and antipyretics (65%). The existence of a vaccine is not well known and represented 16.79%;the use of impregnated mosquito net is more cited as a secondary means of prevention, 77.86%. <strong>Conclusion: </strong>Dengue is not well known by the health professionals involved in the survey. Nevertheless, physicians have a more or less good knowledge compared to paramedics. In practice, the diagnosis of dengue is often not mentioned.展开更多
Bryophytes are broadly used as bioindicators. However, the internal distribution of accumulated elements in the moss tissue is little known. Sampling was carried out in The West Carpathians, Slovakia, in autumn 2012. ...Bryophytes are broadly used as bioindicators. However, the internal distribution of accumulated elements in the moss tissue is little known. Sampling was carried out in The West Carpathians, Slovakia, in autumn 2012. Seven replicates have been used. The samples were analyzed by XRF Spectrometer Delta Classic. S, Pb, K, Ca, Cr, Mn, Fe, Cu, Rb, Sr, Mo, Ba and Zn were determined. For ordination analysis we used principal component analysis, statistical graphics system STATISTICA have been used for the correlation analysis and for analysis of variance. Results show that sulphur, zinc, chromium, manganese, molybdenum, kalcium and copper are preferentially accumulated in the capsula. While lead favors gametophyte, potassium and strontium prefer accumulation in sporophyte. Iron significantly accumulates in the more-year segments, while zinc in the stems. Copper, chromium and sulphur are accumulated preferentially in The Fatra Mts.展开更多
Seven new aromatic polyketides, communols A-G （1-7）, were isolated and identified from the fermentation broth of Penicillium commune 518, a marine-derived fungus associated with the Gorgonian, Muricella abnormalis. ...Seven new aromatic polyketides, communols A-G （1-7）, were isolated and identified from the fermentation broth of Penicillium commune 518, a marine-derived fungus associated with the Gorgonian, Muricella abnormalis. The new structures of 1-7 were determined by spectroscopic analysis and X-ray single crystal diffraction. Among them, communol D （4） was the first example of a naturally occurring aromatic polyketide with a sulfoxide group from marine thngi. Compounds 1, 6, and 7 all showed moderate antimicrobial activities against Escherichia coli and Enterobacter aerogenes with MIC values of 4.1/16.4, 6.4/25.8, and 23.8/23.8μmoloL^-1, respectively.展开更多
is an investigative report on 'contracting output quotas to households under unified management' as a form of the system of job responsibility in agricultural production being implemented in various accounting...is an investigative report on 'contracting output quotas to households under unified management' as a form of the system of job responsibility in agricultural production being implemented in various accounting units of people’s communes. Actually, this form is a synthesis of unified collective management and decentralized management by individual commune member households, on the basis of collective ownership of the means of production. According to our survey, the productive forces within each accounting unit are multileveled; so much so that neither completely unified collective management, nor completely decentralized management by commune member households is suitable for the productive forces’ conditions. Nor is the exclusive use of either form of management conducive to the most effective promotion of agricultural production and the development of a diversified economy. Only a combination of the two can bring into play the productive forces of both the collective and the commune member households and mobilize the initiative of each.展开更多
基金supported by the State Administration for Market Regulation Science and Technology Plan Project under Grant 2022MK192.
文摘Human immortal keratinocyte cells(HaCaT)are induced with UVB to establish an injury model.This model is utilized to investigate whether oat bran fermentation broth(OBF)has a reparative effect on skin inflammation and damage to the skin barrier caused by UVB irradiation.The results show that compared with unfermented oat bran(OB),OBF exhibits higher structural homogeneity,increased molecular weight size,active substances content,and in vitro antioxidant activity.OBF has a scavenging effect on excess reactive oxygen species(ROS)and increases the intracellular levels of antioxidant enzymes.It was found that OBF has a stronger inhibitory effect on the release of inflammatory factors than OB.It increases the synthesis of AQP3 and FLG proteins while decreasing the secretion of KLK-7.OBF can inhibit the transcription level of inflammatory factors by suppressing the JAK/STAT signaling pathway.Safety experiments demonstrate that OBF has a high safety profile.
基金supported by the Program of the National Natural Science Foundation of China(31872519)General Project of Jilin Provincial Department of Science and Technology(20230101247JC)the Open Research Fund of Engineering Research Center of Bioreactor and Pharmaceutical Development,Ministry of Education.(KF202002).
文摘This study aimed to explore the protective effect and potential mechanism of Nostoc commune Vauch.polysaccharide(NCVP)on lead(Pb)-poisoning mice.NCVP improved Pb-induced hepatorenal toxicity and inflammatory responses and modulated key indicators of antioxidant capacity.Moreover,the down-regulation of critical proteins of the Nrf2 pathway induced by Pb could be reversed after NCVP intervention.In addition,NCVP maintained the diversity of gut bacteriobiota and restored the relative abundance of f_Prevotellaceae,g_Alloprevotella,and f_Eubacterium_coprostanoligenes_group reduced by Pb.Also,NCVP regulated the diversity and abundance of gut mycobiota affected by Pb.Specifically,Pb decreased the proportion of pathogenic species(g_Fusarium,p_Basidiomycota,g_Alternaria,g_Aspergillus,and g_Candida)while NCVP increased the abundance of probiotics species(g_Kazachstania and p_Ascomycota).Furthermore,the metabolomic analysis found that NCVP significantly altered a range of microbial metabolites,including porphobilinogen,cromakalim,salidroside,and trichostatin A,which has significant associations with specific gut bacteriobiota or mycobiota.These altered metabolites are involved in primary bile acid biosynthesis,metabolism of xenobiotics by cytochrome P450,lysine degradation,and other metabolic pathways.Overall,our findings indicate that NCVP might be an excellent natural product for eliminating Pb-induced hepatorenal toxicity,possibly by regulating gut bacteriome,mycobiome and metabolome.
文摘The objectives of this work were to evaluate the surgical activities carried out in the general surgery department of the Reference Health Center of Commune I of Bamako, to describe the sociodemographic characteristics of the operated patients, to determine the main pathologies encountered and to evaluate qualitatively the result of the treatment. In order to improve performance, and the quality of care, and to identify common pathologies in the surgical department, we undertook a retrospective study on surgical activities from January 2009 to December 2010. At the end of this study, out of 474 men and 187 women (equal sex ratio 2.53);we were able to determine the frequency of surgical pathologies. Farmers, housewives and pupils/students were the most represented with 25.9% respectively;20% and 13.3%. The most frequently observed pathologies were wall hernia (44.8%), prostate adenoma (12%) and acute appendicitis (10.5%). The average length of hospitalization was 3.43 days. Infectious complications affected 25 patients (3.8% of cases) and a death rate of 0.45% (i.e. 3 patients). The average cost of care was 53,500 FCFA. Indeed, the reality of surgical practice in health centers was not the same because of the level of skills of practicing surgeons.
基金Supported by the Ministry of Science and Technology(No.2010CB833802)the Knowledge Innovation Program of Chinese Academy of Sciences(No.KSCX2-EW-G-12B)the National Natural Science Foundation of China(No.30910103914)
文摘Bioassay-guided fractionation of the crude extract from Penicillium commune SD-118, a fungus obtained from a deep-sea sediment sample, resulted in the isolation of a known antibacterial compound, xanthocillin X (1), and 14 other known compounds comprising three steroids (2-4), two ceramides (5 and 6), six aromatic compounds (7-12), and three alkaloids (13-15). Xanthocillin X (1) was isolated for the first time from a marine fungus. In the bioassay, xanthocillin X (1) displayed remarkable antimicrobial activity against Staphylococcus aureus and Escherichia coli, and significant cytotoxicity against MCF-7, HepG2, H460, Hela, Du145, and MDA-MB-231 cell lines. Meleagrin (15) exhibited cytotoxicity against HepG2, Hela, Du145, and MDA-MB-231 cell lines. This is the first report of the cytotoxicity of xanthocillin X (1).
基金partly supported by Ministry of Science and Technology of China(No.2013CB911300)National Natural Science Foundation of China(Nos.81225024,31025025,31260208,and U1132601)
文摘The present study was designed to search for compounds with analgesic activity from the Schizophyllum commune(SC), which is widely consumed as edible and medicinal mushroom world. Thin layer chromatography(TLC), tosilica gel column chromatography, sephadex LH 20, and reverse-phase high performance liquid chromatography(RP-HPLC) were used to isolate and purify compounds from SC. Structural analysis of the isolated compounds was based on nuclear magnetic resonance(NMR). The effects of these compounds on voltage-gated sodium(NaV) channels were evaluated using patch clamp. The analgesic activity of these compounds was tested in two types of mouse pain models induced by noxious chemicals. Five phenolic acids identified from SC extracts in the present study included vanillic acid, m-hydroxybenzoic acid, o-hydroxybenzeneacetic acid, 3-hydroxy-5-methybenzoic acid, and p-hydroxybenzoic acid. They inhibited the activity of both tetrodotoxin-resistant(TTX-r) and tetrodotoxin-sensitive(TTX-s) NaV channels. All the compounds showed low selectivity on NaV channel subtypes. After intraperitoneal injection, three compounds of these compounds exerted analgesic activity in mice. In conclusion, phenolic acids identified in SC demonstrated analgesic activity, facilitating the mechanistic studies of SC in the treatment of neurasthenia.
基金Special Basic Cooperative Research Programs of Yunnan Provincial Undergraduate Universities(Grant No.2019FH001-109)Undergraduates Innovative Experiment Project from MOE(Grant Nos.202010684016,202110684004)。
文摘The fruits of Pyracantha fortuneana(Maxim.)Li were fermented with pure fungi to improve their antioxidant capacity.The antioxidant activities of fermented and non-fermented P.fortuneana fruits were estimated by 1,1-diphenyl-2-picrylhydrazyl(DPPH)and 2,2'-azinobis-(3-ethylbenzthiazoline-6-sulphonate)(ABTS)radical scavenging activities,as well as ferric ion reducing antioxidant power(FRAP).The total phenolic content(TPC)and total flavonoid content(TFC)were also determined.Additionally,the changes caused by fermentation were characterized by proton nuclear magnetic resonance spectroscopy(1H NMR).The results showed that P.fortuneana fruits fermented with Rhizopus oryzae and Penicillium commune exhibited stronger antioxidant activity compared with the non-fermented material.Correlation analysis indicated that the increased activity might be ascribed to the improvement of TPC.Chemical characterization of 1H NMR implied that the increased TPC and antioxidant activity might be attributed to some of the phenolic and flavonoid glycosides hydrolyzed to new components during fermentation.The present study was the first report of the fermentation of P.fortuneana fruit with pure fungal strains and suggested that fermentation of P.fortuneana fruits with R.oryzae and P.commune was an effective alternative approach to increase antioxidant,and P.fortuneana fruit fermented by R.oryzae and P.commune might be the new alternative of natural antioxidants.
文摘The effects of La(NO3)(3) on the liquid culture and the contents of amino acids of Nostoc commune flagelliforme cells were studied. It is indicated from the experimental results that the growth of Nostoc commune flagelliforme cells can be stimulated by La(NO3)(3) at the concentrations from 1 to 200 mg.L-1, and among them the treatment of 100 mg.L-1 La(NO3)(3) has the largest A(670) and dry weight which increased by 64.9% and 61.1%, respectively as compared with the control, and La(NO3)(3) can also influence the contents of some kinds of amino acids in Nostoc commune flagelliforme cells. The contents of aspartic acid and glutamic acid decreased by 59.9% and 57.9%, respectively, and that of proline increased by 24.5% with the treatment of 200 mg.L-1 La(NO3)(3).
基金Supported by the National Natural Science Foundation of China(30700084)
文摘Using Nostoc commune as experiment target,the effect of CO 2 with different concentrations in atmosphere and dosage of UV-B on photosynthetic pigment chlorophyll a （Chl a）,carotenoids,phycoerythrin,phycocyanin,allophycocyanin and UV-B masking materials was studied.The results showed that high dosage of UV-B radiation inhibited the synthesis process of Chl a,phycoerythrin,phycocyanin and allophycocyanin,but promoted the synthesis of carotenoids,MAAs and scytonemin,thus the negative effect produced by UV-B radiation was reduced and the resistance of Nostoc commune against UV-B radiation was enhanced.
基金National Natural Science Foundation of China(Grant Nos.31370372,81573316,31270395,31570361,and 81202423)the National Science and Technology Project of China(Grant Nos.2011ZX09102-004 and 2013ZX09103001-020)
文摘Phytochemical investigation of the endophytic fungus Penicillium commune led to the isolation of nine known compounds. Based on the spectroscopic and chemical properties, their structures were identified as 6-acetyl-2α,5-dihydroxy-2-（2-hydroxypropyl）-3α,8-dimethylchroman（1）, communol B（2）, communol E（3）, communol G（4）, 2-hydroxy-4-phenylquinoline（5）, viridicatol（6）, 3-O-methylviridicatin（7）, trans-ferulic acid（8）, 1,2-benzenediol,4-（2-methoxyethenyl）（9）. Compounds 1 and 5–9 were isolated from this fungus for the first time.
基金National Nature Science Foundation of China (No.31171662)"973"National Key Basic Research and Development Program(No.2012CB518202)+1 种基金Project of Qinghai Development of Science and Technology(No.2011-N-150)Key Project of GLD of PLA
文摘The schizophyllan from Schizophyllum commune was purified and characterized. The crude schizo-phyllan was obtained from Schizophyllum commune fermentation broth by decoloration with activated carbon,followed by deproteinization with Sevag method and ethanol precipitation. The pure schizophyllan was obtained by gel filtration chromatography with Sephacryl S-500,and its molecular characteristics were examined. The result showed that the molecular weight was 3.8×104 ,and the schizophyllan consisted of glucose with β-(1→6)-glucosidic linkages.
文摘Artificial induction of labor (AIL) is the set of techniques intended to induce childbirth in order to reduce maternal-fetal mortality. The aim of this paper was to study the maternal and fetal prognosis after the artificial induction of labor with misoprostol. It was a descriptive prospective study conducted from September 1, 2018 to February 28, 2019 at the maternity unit of the Referral Health Center (RHC) in commune I of Bamako. It was about any pregnant woman at term who had received misoprostol as part of the artificial induction of labor. 102 cases of artificial induction with misoprostol 200 μg were collected out of 3641 deliveries, or a frequency of 2.8%. The age group 28 - 32 years accounted for 56.86% with an average age of 28.8 years. Multiparous represented 54.90% of the number. The history of diabetes and hypertension involved 37.25% of the pregnant women. The main indication was premature rupture of membranes (PRM) (40.2%). Induction was performed between the 37</span><sup><span style="font-family:Verdana;">th</span></sup><span style="font-family:Verdana;"> and 41</span><sup><span style="font-family:Verdana;">st</span></sup><span style="font-family:Verdana;"> week of amenorrhea (69.6%). The trigger index according to BISHOP was greater than 7 (50.98%). Induction was done only with 3/4 of misoprostol tablet 200 μg (43.14%). The average labor time was 7 hours (89.22%). The vaginal birth concerned 92.16% of pregnant women. An APGAR10 was noted in 97.85% of newborns after 05 minutes. AIL with misoprostol was practiced at the Center. The main indication was the premature rupture of the membranes. It improves the maternal and fetal prognosis.
文摘Land resource depletion causes a range of consequences,such as threats to food security,increasing poverty,and social unrest.Therefore,reasonable and effective land use has become a crucial issue of the nation and localities nationwide.Research results in Tan Binh commune,Dak Doa district,Gia Lai Province in Vietnam showed that long-term industrial and agricultural land occupies most of the commune's agroforestry land.The study also had shown that there are six common land use types(LUTs)in the commune.In particular,the LUT of pepper cultivation had the highest economic benefit,whereas the LUT of coffee brought the highest social benefit.However,the LUT of chayote was the highest in overall benefits with Ect of 0.93;thus,this LUT should be widely applied throughout Tan Binh commune.The research results were a crucial scientific basis for proposing a group of suitable plants to local natural conditions to bring effective and sustainable efficiency in land-use types.
文摘<strong>Background:</strong> Dengue is under-reported in the indicators and directories of health statistics in Togo and the diagnostic hypothesis of dengue during medical consultations is often not mentioned. <strong>Objective:</strong> The objective is to assess healthcare workers’ knowledge of dengue and its management in public health centers in the health district of Lomé Commune. <strong>Materials and Methods: </strong>This is a cross-sectional and descriptive study that took place from November 1, 2019 to February 29, 2020 in the health district of Lomé Commune. <strong>Results: </strong>This study involved 280 healthcare workers out of 310 concerned, <em>i.e.</em>, a participation rate of 90.32%, distributed over the 22 public health centers of the health district of Lomé Commune. The age of the healthcare workers ranged from 18 to 58 years with an average age of 33 years (±8.14). The age group [25 - 35 years] was more represented with 53.20%. Physicians were more represented with 46.07%. The majority of healthcare professionals were aware of the existence of dengue (90.36%). The essential clinical signs were more or less known, especially by the physicians. Dengue hemorrhagic fever was the most known clinical form and represented 70%. Treatment was symptomatic according to the majority (63.21%) and was based on analgesics (71.43%) and antipyretics (65%). The existence of a vaccine is not well known and represented 16.79%;the use of impregnated mosquito net is more cited as a secondary means of prevention, 77.86%. <strong>Conclusion: </strong>Dengue is not well known by the health professionals involved in the survey. Nevertheless, physicians have a more or less good knowledge compared to paramedics. In practice, the diagnosis of dengue is often not mentioned.
基金the Norwegian Financial Mechanisms of the research project SK 1551/2012
文摘Bryophytes are broadly used as bioindicators. However, the internal distribution of accumulated elements in the moss tissue is little known. Sampling was carried out in The West Carpathians, Slovakia, in autumn 2012. Seven replicates have been used. The samples were analyzed by XRF Spectrometer Delta Classic. S, Pb, K, Ca, Cr, Mn, Fe, Cu, Rb, Sr, Mo, Ba and Zn were determined. For ordination analysis we used principal component analysis, statistical graphics system STATISTICA have been used for the correlation analysis and for analysis of variance. Results show that sulphur, zinc, chromium, manganese, molybdenum, kalcium and copper are preferentially accumulated in the capsula. While lead favors gametophyte, potassium and strontium prefer accumulation in sporophyte. Iron significantly accumulates in the more-year segments, while zinc in the stems. Copper, chromium and sulphur are accumulated preferentially in The Fatra Mts.
文摘Seven new aromatic polyketides, communols A-G （1-7）, were isolated and identified from the fermentation broth of Penicillium commune 518, a marine-derived fungus associated with the Gorgonian, Muricella abnormalis. The new structures of 1-7 were determined by spectroscopic analysis and X-ray single crystal diffraction. Among them, communol D （4） was the first example of a naturally occurring aromatic polyketide with a sulfoxide group from marine thngi. Compounds 1, 6, and 7 all showed moderate antimicrobial activities against Escherichia coli and Enterobacter aerogenes with MIC values of 4.1/16.4, 6.4/25.8, and 23.8/23.8μmoloL^-1, respectively.
文摘is an investigative report on 'contracting output quotas to households under unified management' as a form of the system of job responsibility in agricultural production being implemented in various accounting units of people’s communes. Actually, this form is a synthesis of unified collective management and decentralized management by individual commune member households, on the basis of collective ownership of the means of production. According to our survey, the productive forces within each accounting unit are multileveled; so much so that neither completely unified collective management, nor completely decentralized management by commune member households is suitable for the productive forces’ conditions. Nor is the exclusive use of either form of management conducive to the most effective promotion of agricultural production and the development of a diversified economy. Only a combination of the two can bring into play the productive forces of both the collective and the commune member households and mobilize the initiative of each.
