The extensive use of nanoparticles(NPs)in diverse applications causes their localization to aquatic habitats,affecting the metabolic products of primary producers in aquatic ecosystems,such as algae.Synthesized calciu...The extensive use of nanoparticles(NPs)in diverse applications causes their localization to aquatic habitats,affecting the metabolic products of primary producers in aquatic ecosystems,such as algae.Synthesized calcium oxide nanoparticles(CaO NPs)are of the scarcely studied NPs.Thus,the current work proposed that the exposure to CaO NPs may instigate metabolic pathway to be higher than that of normally growing algae,and positively stimulate algal biomass.In this respect,this research was undertaken to study the exposure effect of CaO NPs(0,20,40,60,80,and 100μg mL^(−1))on the growth,photosynthesis,respiration,oxidative stress,antioxidants,and lipid production of the microalga Coccomyxa chodatii SAG 216-2.The results showed that the algal growth concomitant with chlorophyll content,photosynthesis,and calcium content increased in response to CaO NPs.The contents of biomolecules such as proteins,amino acids,and carbohydrates were also promoted by CaO NPs with variant degrees.Furthermore,lipid production was enhanced by the applied nanoparticles.CaO NPs induced the accumulation of hydrogen peroxide,while lipid peroxidation was reduced,revealing no oxidative behavior of the applied nanoparticles on alga.Also,CaO NPs have a triggering effect on the antioxidant enzymes such as superoxide dismutase,catalase,ascorbate peroxidase,and guaiacol peroxidase.The results recommended the importance of the level of 60μg mL^(−1) CaO NPs on lipid production(with increasing percentage of 65%compared to control)and the highest dry matter acquisition of C.chodatii.This study recommended the feasibility of an integrated treatment strategy of CaO NPs in augmenting biomass,metabolic up-regulations,and lipid accumulation in C.chodatii.展开更多
在75g/L浓度的硫酸镁溶液中发现了一株生长良好的单细胞绿藻。为了解这株特殊微藻的系统进化学分类地位和潜在利用价值,本文结合了分子系统学方法和形态特征,并测定其不同盐环境下的光合效率,以探讨其对于硫酸镁盐的耐受机理。结果表明...在75g/L浓度的硫酸镁溶液中发现了一株生长良好的单细胞绿藻。为了解这株特殊微藻的系统进化学分类地位和潜在利用价值,本文结合了分子系统学方法和形态特征,并测定其不同盐环境下的光合效率,以探讨其对于硫酸镁盐的耐受机理。结果表明,基于该藻株18S及ITS r DNA序列构建的系统进化树确认其隶属于胶球藻属。该株藻细胞为椭圆形、具有较小的长宽比、色素体多为分叶的2瓣和无明显胶被等特征与胶球形胶球藻较接近,系统发育分析显示其与伪胶球藻株系和与地衣Peltigerales共生株系亲缘关系较近。对该藻不同培养条件下的耐受特征做出初步探索,结果表明其对硫酸镁有着独特的单盐抗性,并可以耐受蒸馏水环境。这些特性表明该藻株有良好的抗杂藻污染特性,具有潜在应用前景。展开更多
文摘The extensive use of nanoparticles(NPs)in diverse applications causes their localization to aquatic habitats,affecting the metabolic products of primary producers in aquatic ecosystems,such as algae.Synthesized calcium oxide nanoparticles(CaO NPs)are of the scarcely studied NPs.Thus,the current work proposed that the exposure to CaO NPs may instigate metabolic pathway to be higher than that of normally growing algae,and positively stimulate algal biomass.In this respect,this research was undertaken to study the exposure effect of CaO NPs(0,20,40,60,80,and 100μg mL^(−1))on the growth,photosynthesis,respiration,oxidative stress,antioxidants,and lipid production of the microalga Coccomyxa chodatii SAG 216-2.The results showed that the algal growth concomitant with chlorophyll content,photosynthesis,and calcium content increased in response to CaO NPs.The contents of biomolecules such as proteins,amino acids,and carbohydrates were also promoted by CaO NPs with variant degrees.Furthermore,lipid production was enhanced by the applied nanoparticles.CaO NPs induced the accumulation of hydrogen peroxide,while lipid peroxidation was reduced,revealing no oxidative behavior of the applied nanoparticles on alga.Also,CaO NPs have a triggering effect on the antioxidant enzymes such as superoxide dismutase,catalase,ascorbate peroxidase,and guaiacol peroxidase.The results recommended the importance of the level of 60μg mL^(−1) CaO NPs on lipid production(with increasing percentage of 65%compared to control)and the highest dry matter acquisition of C.chodatii.This study recommended the feasibility of an integrated treatment strategy of CaO NPs in augmenting biomass,metabolic up-regulations,and lipid accumulation in C.chodatii.
文摘在75g/L浓度的硫酸镁溶液中发现了一株生长良好的单细胞绿藻。为了解这株特殊微藻的系统进化学分类地位和潜在利用价值,本文结合了分子系统学方法和形态特征,并测定其不同盐环境下的光合效率,以探讨其对于硫酸镁盐的耐受机理。结果表明,基于该藻株18S及ITS r DNA序列构建的系统进化树确认其隶属于胶球藻属。该株藻细胞为椭圆形、具有较小的长宽比、色素体多为分叶的2瓣和无明显胶被等特征与胶球形胶球藻较接近,系统发育分析显示其与伪胶球藻株系和与地衣Peltigerales共生株系亲缘关系较近。对该藻不同培养条件下的耐受特征做出初步探索,结果表明其对硫酸镁有着独特的单盐抗性,并可以耐受蒸馏水环境。这些特性表明该藻株有良好的抗杂藻污染特性,具有潜在应用前景。
