Photocatalytic technology has been widely adopted to address the issue of air pollution. The separation of photogenerated carriers and the activation of reactants on catalyst surface are the main factors that affect t...Photocatalytic technology has been widely adopted to address the issue of air pollution. The separation of photogenerated carriers and the activation of reactants on catalyst surface are the main factors that affect the photocatalytic efficiency. Here, the phosphate/potassium(PO_4/K) cofunctionalized carbon nitride(labeled as PO_4-CN-K) was synthesized via a one-step in situ copyrolysis of thiourea and potassium phosphate. The unique electronic structure of PO_4-CN-K could significantly improve the performance of photocatalytic NO purification. The enhanced activity of PO_4-CN-K can be attributed to the promoted activation capacity for O_2, NO and H_2O on the catalyst surface,the decreased of carriers recombination, benefiting from the co-functionalization of phosphate groups on the surface of CN and the construction of K channels between CN layers. The photocatalytic NO conversion pathway is disclosed through time-dependent in situ FT-IR, indicating that PO_4-CN-K can efficiently convert NO molecules into harmless nitrate via the NO→NO+→nitrate/nitrite routes. The research provides a novel strategy to impel the development of photocatalytic technology for efficient air purification.展开更多
MicroRNAs （miRNAs） interact with their target mRNAs and regulate biological processes at post-transcriptional level. While one miRNA can target many mRNAs, a single mRNA can also be targeted by a set of miRNAs. The ...MicroRNAs （miRNAs） interact with their target mRNAs and regulate biological processes at post-transcriptional level. While one miRNA can target many mRNAs, a single mRNA can also be targeted by a set of miRNAs. The targeted mRNAs may be involved in different biological processes that are described by gene ontology （GO） terms. The major challenges involved in analyzing these multitude regulations include identification of the combinatorial regulation of miRNAs as well as determination of the co-functionally-enriched miRNA pairs. The C2Analyzer： Co- target Co-function Analyzer, is a Perl-based, versatile and user-friendly web tool with online instructions. Based on the hypergeometric analysis, this novel tool can determine whether given pairs of miRNAs are co-functionally enriched. For a given set of GO term（s）, it can also identify the set of miRNAs whose targets are enriched in the given GO term（s）. Moreover, C2Analyzer can also identify the co-targeting miRNA pairs, their targets and GO processes, which they are involved in. The miRNA miRNA co-functional relationship can also be saved as a .txt file, which can be used to further visualize the co-functional network by using other software like Cytoscape. C2Analyzer is freely available at www.bioinformatics.org/c2analyzer.展开更多
研究木质素对烤烟生长和土壤微生物的影响,为木质素的资源化利用提供参考。采用烤烟盆栽试验,添加土壤重量2‰和5‰的木质素作为处理组(L2和L3),并以不添加木质素作为对照处理(L1),在测定烤烟农艺性状和土壤pH的基础上,结合高通量测序...研究木质素对烤烟生长和土壤微生物的影响,为木质素的资源化利用提供参考。采用烤烟盆栽试验,添加土壤重量2‰和5‰的木质素作为处理组(L2和L3),并以不添加木质素作为对照处理(L1),在测定烤烟农艺性状和土壤pH的基础上,结合高通量测序和共现网络分析的方法,研究木质素对烤烟生长和土壤微生物的影响,并使用FAPROTAX数据库和FUNGuild进行细菌和真菌功能组进行分析。结果显示:与L1处理相比,L2和L3处理烤烟的株高分别增加了10.1%和15.6%,上部烟叶长分别增加了6.9%和12.2%;成熟前期L2和L3处理土壤的pH显著降低了0.38。Alpha多样性分析和PCoA分析表明,木质素添加对土壤微生物多样性指数(Shannon index)、丰富度指数(Chao1 index)和β多样性均无显著影响。门水平上微生物群落组成分析表明,木质素的加入显著减少了成熟前期拟杆菌门、芽单胞菌门和硝化螺旋菌门及上部烟叶成熟期酸杆菌门的相对丰度;真菌中明显增加了成熟前期和上部烟叶成熟期子囊菌门和担子菌门的相对丰度,减少了被孢霉门的相对丰度。微生物共现网络分析结果表明,木质素使土壤微生物共现网络的复杂性降低,并增加了细菌共现网络的稳定性、削弱了真菌群落之间的合作关系,同时木质素的添加使放线菌成为细菌共现网络的主导菌群之一。功能预测结果表明,木质素的添加均显著增加了硝酸盐呼吸作用和氮呼吸作用的相关基因丰度;显著降低了动物病原-内生-附生-真菌病菌-植物病原-木腐菌的基因相对丰度。相关性分析结果表明,Saccharibacteria genera incertae sedis,Gemmatimonas和Minutisphaera分别在不同时期与烤烟株高和上部叶叶长均存在正相关关系。总体上,木质素的添加改变了植烟土壤中微生物群落结构组成,其中Saccharibacteria genera incertae sedis,Gemmatimonas和Minutisphaera对烤烟株高和上部烟叶叶长的提高可能具有重要作用。展开更多
基金supported by the National Natural Science Foundation of China (Nos.21822601, 21777011 and 21501016)the Innovative Research Team of Chongqing (No. CXTDG201602014)+1 种基金the Natural Science Foundation of Chongqing (No. cstc2017jcyjBX0052)the Plan for "National Youth36 Talents" of the Organization Department of the Central Committee
文摘Photocatalytic technology has been widely adopted to address the issue of air pollution. The separation of photogenerated carriers and the activation of reactants on catalyst surface are the main factors that affect the photocatalytic efficiency. Here, the phosphate/potassium(PO_4/K) cofunctionalized carbon nitride(labeled as PO_4-CN-K) was synthesized via a one-step in situ copyrolysis of thiourea and potassium phosphate. The unique electronic structure of PO_4-CN-K could significantly improve the performance of photocatalytic NO purification. The enhanced activity of PO_4-CN-K can be attributed to the promoted activation capacity for O_2, NO and H_2O on the catalyst surface,the decreased of carriers recombination, benefiting from the co-functionalization of phosphate groups on the surface of CN and the construction of K channels between CN layers. The photocatalytic NO conversion pathway is disclosed through time-dependent in situ FT-IR, indicating that PO_4-CN-K can efficiently convert NO molecules into harmless nitrate via the NO→NO+→nitrate/nitrite routes. The research provides a novel strategy to impel the development of photocatalytic technology for efficient air purification.
基金University Grants Commission(Research Fellowship for Meritorious Students in Science),Government of India for financial supportDistributed Information Center,University of Calcutta for computational facilities.SK acknowledges Centre of Excellenceon Systems Biology and Biomedical Engineering forpartial support
文摘MicroRNAs （miRNAs） interact with their target mRNAs and regulate biological processes at post-transcriptional level. While one miRNA can target many mRNAs, a single mRNA can also be targeted by a set of miRNAs. The targeted mRNAs may be involved in different biological processes that are described by gene ontology （GO） terms. The major challenges involved in analyzing these multitude regulations include identification of the combinatorial regulation of miRNAs as well as determination of the co-functionally-enriched miRNA pairs. The C2Analyzer： Co- target Co-function Analyzer, is a Perl-based, versatile and user-friendly web tool with online instructions. Based on the hypergeometric analysis, this novel tool can determine whether given pairs of miRNAs are co-functionally enriched. For a given set of GO term（s）, it can also identify the set of miRNAs whose targets are enriched in the given GO term（s）. Moreover, C2Analyzer can also identify the co-targeting miRNA pairs, their targets and GO processes, which they are involved in. The miRNA miRNA co-functional relationship can also be saved as a .txt file, which can be used to further visualize the co-functional network by using other software like Cytoscape. C2Analyzer is freely available at www.bioinformatics.org/c2analyzer.
基金supported by the National Science and Technology Major Project of the Ministry of Science and Technology of China(Brain Science and Brain-Like Intelligence Technology,No.2025ZD0214902)the National Natural Science Foundation of China(No.32271047,32471054)。
文摘研究木质素对烤烟生长和土壤微生物的影响,为木质素的资源化利用提供参考。采用烤烟盆栽试验,添加土壤重量2‰和5‰的木质素作为处理组(L2和L3),并以不添加木质素作为对照处理(L1),在测定烤烟农艺性状和土壤pH的基础上,结合高通量测序和共现网络分析的方法,研究木质素对烤烟生长和土壤微生物的影响,并使用FAPROTAX数据库和FUNGuild进行细菌和真菌功能组进行分析。结果显示:与L1处理相比,L2和L3处理烤烟的株高分别增加了10.1%和15.6%,上部烟叶长分别增加了6.9%和12.2%;成熟前期L2和L3处理土壤的pH显著降低了0.38。Alpha多样性分析和PCoA分析表明,木质素添加对土壤微生物多样性指数(Shannon index)、丰富度指数(Chao1 index)和β多样性均无显著影响。门水平上微生物群落组成分析表明,木质素的加入显著减少了成熟前期拟杆菌门、芽单胞菌门和硝化螺旋菌门及上部烟叶成熟期酸杆菌门的相对丰度;真菌中明显增加了成熟前期和上部烟叶成熟期子囊菌门和担子菌门的相对丰度,减少了被孢霉门的相对丰度。微生物共现网络分析结果表明,木质素使土壤微生物共现网络的复杂性降低,并增加了细菌共现网络的稳定性、削弱了真菌群落之间的合作关系,同时木质素的添加使放线菌成为细菌共现网络的主导菌群之一。功能预测结果表明,木质素的添加均显著增加了硝酸盐呼吸作用和氮呼吸作用的相关基因丰度;显著降低了动物病原-内生-附生-真菌病菌-植物病原-木腐菌的基因相对丰度。相关性分析结果表明,Saccharibacteria genera incertae sedis,Gemmatimonas和Minutisphaera分别在不同时期与烤烟株高和上部叶叶长均存在正相关关系。总体上,木质素的添加改变了植烟土壤中微生物群落结构组成,其中Saccharibacteria genera incertae sedis,Gemmatimonas和Minutisphaera对烤烟株高和上部烟叶叶长的提高可能具有重要作用。
