Objective To compare the performance of nextgeneration sequencing(NGS)and Sanger sequencing in investigating somatic hypermutation(SHM)status of immunoglobulin heavy chain variable region(IGHV)genes.It specifically fo...Objective To compare the performance of nextgeneration sequencing(NGS)and Sanger sequencing in investigating somatic hypermutation(SHM)status of immunoglobulin heavy chain variable region(IGHV)genes.It specifically focuses on identifying key factors contributing to discrepancies between the two methods,particularly under complex clonal backgrounds,to inform optimized strategies for clinical application.展开更多
文摘Objective To compare the performance of nextgeneration sequencing(NGS)and Sanger sequencing in investigating somatic hypermutation(SHM)status of immunoglobulin heavy chain variable region(IGHV)genes.It specifically focuses on identifying key factors contributing to discrepancies between the two methods,particularly under complex clonal backgrounds,to inform optimized strategies for clinical application.
