High-throughput sequencing in identifying somatic hypermutation in immunoglobulin heavy chain variable regions with complex clonal backgrounds
摘要
Objective To compare the performance of nextgeneration sequencing(NGS)and Sanger sequencing in investigating somatic hypermutation(SHM)status of immunoglobulin heavy chain variable region(IGHV)genes.It specifically focuses on identifying key factors contributing to discrepancies between the two methods,particularly under complex clonal backgrounds,to inform optimized strategies for clinical application.
