BACKGROUND As a non-coding RNA molecule,circular RNAs(circRNAs)have significant specificity,and existing data suggest a close relationship between them and the prognosis of patients with gastric cancer(GC).However,thi...BACKGROUND As a non-coding RNA molecule,circular RNAs(circRNAs)have significant specificity,and existing data suggest a close relationship between them and the prognosis of patients with gastric cancer(GC).However,this mechanism has no evidence yet.This article explores the functions of hsa_circRNA_102415 in the malignant behavior and potential downstream signaling of GC cells.The chosen approach is loss of signal and functional gain.AIM To investigate and analyze the relationship between hsa_circRNA_102415 and GC and explore its specific role.Results provide reference for other researchers to develop targeted treatment plans.METHODS The gene expression omnibus(GEO)database can be used to obtain the microarray dataset GSE83521.Data were analyzed using the GEO2R tool to identify differences in circRNAs between normal and GC samples.Quantitative real-time polymerase chain reaction was used to detect differentially expressed genes in GC tissue samples and adjacent cancer tissue samples.GC cells were transfected with small interfering-hsa_circRNA_104415 and plasmid DNA(pcDNA)-hsa_ircRNA_102415.Multiple detection methods,such as Transwell and cell counting kit 8,were used to evaluate cellular physiological activities,including cell invasion and proliferation.The relationship between Wnt family members 2B,microRNA(miR)-4529-5p,etc.,including argonaute 2-RNA immunoprecipitation and luciferase reporter genes was analyzed.Rescue experiments were conducted to analyze and explore the relationship between the malignant behavior of GC cells and hsa_circRNA_102415.RESULTS GEO2R analysis confirmed that hsa_circRNA_102415 had significantly higher expression levels in disease tissues.hsa_circRNA_102415 and miR-4529-5p showed a negative correlation in disease cells,suggesting that hsa_circRNA_102415 upregulated WNT2B expression in GC cells as a competing endogenous RNA for miR-4529-5p.miR-4529-5p mimic or small interfering-WNT2B reversed the effects of pcDNA-hsa_circRNA_102415 or miR-4529-5p inhibitor on cell malignant functions.CONCLUSION miR-4529-5p was used to successfully activate the potential of WNT2B,clarify the role of hsa_circRNA_102415 in GC cells,and provide reference for other researchers to develop targeted treatment plans.展开更多
Endothelial-mesenchymal transition(EndoMT)is associated with damage to blood-brain barrier(BBB)integrity.Circular RNAs(circRNAs)are highly expressed in the brain and are involved in brain diseases;however,whether circ...Endothelial-mesenchymal transition(EndoMT)is associated with damage to blood-brain barrier(BBB)integrity.Circular RNAs(circRNAs)are highly expressed in the brain and are involved in brain diseases;however,whether circR NAs regulate the EndoM T in the brain remains unknown.Our study demonstrated that circH ECW2 regulated the EndoM T by directly binding to MIR30 D,a significantly downregulated miR NA from miR NA profiling,which subsequently caused an increased expression of ATG5.These findings shed new light on the understanding of the noncanonical role of ATG5 in the EndoMT induced by methamphetamine or lipopolysaccharide.The in vivo relevance was confirmed as microinjection of circHecw2 siRNA lentivirus into the mouse hippocampus suppressed the EndoMT induced by LPS.These findings provide novel insights regarding the contribution of circHECW2 to the nonautophagic role of ATG5 in the EndoMT process in the context of drug abuse and the broad range of neuroinflammatory disorders.展开更多
Based on our 2D BEM software THBEM2 which can be applied to thesimulation of an elastic body with randomly distributed identicalcircular holes, a scheme of BEM for the simulation of elastic bodieswith randomly distrib...Based on our 2D BEM software THBEM2 which can be applied to thesimulation of an elastic body with randomly distributed identicalcircular holes, a scheme of BEM for the simulation of elastic bodieswith randomly distributed circular inclusions is proposed. Thenumerical examples given show that the bound- ary element method ismore accurate and more effective than the finite element method forsuch a problem. The scheme presented van also be successfully used toestimate the effective elastic properties of composite Materials.展开更多
Under spinning conditions, lubricant on islandic spot patterned M2 steel disc experiences centrifugal and tangential force components. Depending upon the relative position of the spots and the flow of lubricant, accum...Under spinning conditions, lubricant on islandic spot patterned M2 steel disc experiences centrifugal and tangential force components. Depending upon the relative position of the spots and the flow of lubricant, accumulation of lubricant in front of patterned islandic spots creates thrusting to mating part and subsequently reduces contact between the mating couple. Whilst wear debris is likely to be spun off the plateau of the spots to their neighbouring valleys so as to reduce wear. Hence, it gives favorable tribological characteristics. Aiming at verifying such mechanisms, studies were performed on M2 steel disc specimens slid with ASSAB 17 tool steel pin. The M2 steel disc specimens were respectively （i） machined with non-patterned （NP）, （ii） etched to produce in-lined （INE） islandic patterns, and （iii） etched to produce staggered （STE） islandic spot patterns. Results indicated that the INE patterned discs gave most favorable wear characteristics, the NP of the worse characteristics whilst the STE ranged in the middle. However, the actual contact mechanism leads to the descending sequence of favorable friction behaviors nominally as： NP, INE and STE.展开更多
In array signal processing, 2-D spatial-spectrum estimation is required to determine DOA of multiple signals. The circular array of sensors is found to possess several nice properties for DOA estimation of wide-band s...In array signal processing, 2-D spatial-spectrum estimation is required to determine DOA of multiple signals. The circular array of sensors is found to possess several nice properties for DOA estimation of wide-band sources. C. U. Padmini, et al.(1994) had suggested that the frequency-direction ambiguity in azimuth estimation of wide-baud signals received by a uniform linear array (ULA) can be avoided by using a circular array, even without the use of any delay elements. In 2-D spatial-spectrum estimation for wide-band signals, the authors find that it is impossible to avoid the ambiguity in source frequency-elevation angle pairs using a circular array. In this paper, interpolated circular arrays are used to perform 2-D spatial-spectrum estimation for wide-band sources. In the estimation, a large aperture circular array (Υ】λmin/2) is found to possess superior resolution capability and robustness.展开更多
This paper extends the Non-Circular MUltiple SIgnal Classification(MUSIC)(NC-MUSIC) method for the common array geometries including Uniform Circular Arrays(UCAs) and Uniform Rectangular Arrays(URAs),which enables the...This paper extends the Non-Circular MUltiple SIgnal Classification(MUSIC)(NC-MUSIC) method for the common array geometries including Uniform Circular Arrays(UCAs) and Uniform Rectangular Arrays(URAs),which enables the algorithm to estimate 2-D Direction Of Arrival(DOA).A comparison between UCAs and URAs of NC-MUSIC is made in this paper.The simulations show that the NC-MUSIC method doubles the maximum estimation number of standard MUSIC.Using non-circular signals,the performance of URAs is improved remarkably while the improvement of UCAs is not so significantly.Moreover,the influence of arrays structures on the NC-MUSIC method is discussed.展开更多
Objective:To elucidate the regulatory effects and mechanisms of circular RNA MGAT5(circMGAT5)on the invasive ability of human ovarian cancer(OC)cells.Methods:Human OC SKOV-3 cells were transfected with circMGAT5 inter...Objective:To elucidate the regulatory effects and mechanisms of circular RNA MGAT5(circMGAT5)on the invasive ability of human ovarian cancer(OC)cells.Methods:Human OC SKOV-3 cells were transfected with circMGAT5 interference plasmids.The expression level of circMGAT5 was detected by quantitative real-time polymerase chain reaction(qRT-PCR).The proliferation and invasive abilities of cancer cells were determined using soft agar colony formation assay and Transwell assay,respectively.The expression of matrix metalloproteinase-2(MMP-2)at mRNA and protein levels was detected by qRT-PCR and immunofluorescence,respectively.Results:CircMGAT5 level in the circMGAT5-transfected group was significantly downregulated in a concentration-and time-dependent manner(p<0.005).Soft agar colony formation assay exhibited that the number of colonies formed in the 6.25,12.5,and 25.0 nM siRNA groups was significantly reduced in a concentration-dependent manner(p<0.005).Transwell assay revealed that the number of cells penetrating the filter membrane in the 6.25,12.5,and 25.0 nM siRNA groups was significantly decreased in a concentration-dependent manner(p<0.005).qRT-PCR and immunofluorescence results demonstrated that the mRNA and protein levels of MMP-2 in OC cells of the siRNA-transfected group were significantly decreased.Conclusion:circMGAT5 plays an important role in the invasion of OC cells,and its mechanism may be related to the downregulation of MMP-2 expression.展开更多
文摘BACKGROUND As a non-coding RNA molecule,circular RNAs(circRNAs)have significant specificity,and existing data suggest a close relationship between them and the prognosis of patients with gastric cancer(GC).However,this mechanism has no evidence yet.This article explores the functions of hsa_circRNA_102415 in the malignant behavior and potential downstream signaling of GC cells.The chosen approach is loss of signal and functional gain.AIM To investigate and analyze the relationship between hsa_circRNA_102415 and GC and explore its specific role.Results provide reference for other researchers to develop targeted treatment plans.METHODS The gene expression omnibus(GEO)database can be used to obtain the microarray dataset GSE83521.Data were analyzed using the GEO2R tool to identify differences in circRNAs between normal and GC samples.Quantitative real-time polymerase chain reaction was used to detect differentially expressed genes in GC tissue samples and adjacent cancer tissue samples.GC cells were transfected with small interfering-hsa_circRNA_104415 and plasmid DNA(pcDNA)-hsa_ircRNA_102415.Multiple detection methods,such as Transwell and cell counting kit 8,were used to evaluate cellular physiological activities,including cell invasion and proliferation.The relationship between Wnt family members 2B,microRNA(miR)-4529-5p,etc.,including argonaute 2-RNA immunoprecipitation and luciferase reporter genes was analyzed.Rescue experiments were conducted to analyze and explore the relationship between the malignant behavior of GC cells and hsa_circRNA_102415.RESULTS GEO2R analysis confirmed that hsa_circRNA_102415 had significantly higher expression levels in disease tissues.hsa_circRNA_102415 and miR-4529-5p showed a negative correlation in disease cells,suggesting that hsa_circRNA_102415 upregulated WNT2B expression in GC cells as a competing endogenous RNA for miR-4529-5p.miR-4529-5p mimic or small interfering-WNT2B reversed the effects of pcDNA-hsa_circRNA_102415 or miR-4529-5p inhibitor on cell malignant functions.CONCLUSION miR-4529-5p was used to successfully activate the potential of WNT2B,clarify the role of hsa_circRNA_102415 in GC cells,and provide reference for other researchers to develop targeted treatment plans.
文摘Endothelial-mesenchymal transition(EndoMT)is associated with damage to blood-brain barrier(BBB)integrity.Circular RNAs(circRNAs)are highly expressed in the brain and are involved in brain diseases;however,whether circR NAs regulate the EndoM T in the brain remains unknown.Our study demonstrated that circH ECW2 regulated the EndoM T by directly binding to MIR30 D,a significantly downregulated miR NA from miR NA profiling,which subsequently caused an increased expression of ATG5.These findings shed new light on the understanding of the noncanonical role of ATG5 in the EndoMT induced by methamphetamine or lipopolysaccharide.The in vivo relevance was confirmed as microinjection of circHecw2 siRNA lentivirus into the mouse hippocampus suppressed the EndoMT induced by LPS.These findings provide novel insights regarding the contribution of circHECW2 to the nonautophagic role of ATG5 in the EndoMT process in the context of drug abuse and the broad range of neuroinflammatory disorders.
基金the National Natural Science Foundation of China(No.19772025)
文摘Based on our 2D BEM software THBEM2 which can be applied to thesimulation of an elastic body with randomly distributed identicalcircular holes, a scheme of BEM for the simulation of elastic bodieswith randomly distributed circular inclusions is proposed. Thenumerical examples given show that the bound- ary element method ismore accurate and more effective than the finite element method forsuch a problem. The scheme presented van also be successfully used toestimate the effective elastic properties of composite Materials.
基金the National Natural Science Foundation of China(No. 50575173).
文摘Under spinning conditions, lubricant on islandic spot patterned M2 steel disc experiences centrifugal and tangential force components. Depending upon the relative position of the spots and the flow of lubricant, accumulation of lubricant in front of patterned islandic spots creates thrusting to mating part and subsequently reduces contact between the mating couple. Whilst wear debris is likely to be spun off the plateau of the spots to their neighbouring valleys so as to reduce wear. Hence, it gives favorable tribological characteristics. Aiming at verifying such mechanisms, studies were performed on M2 steel disc specimens slid with ASSAB 17 tool steel pin. The M2 steel disc specimens were respectively （i） machined with non-patterned （NP）, （ii） etched to produce in-lined （INE） islandic patterns, and （iii） etched to produce staggered （STE） islandic spot patterns. Results indicated that the INE patterned discs gave most favorable wear characteristics, the NP of the worse characteristics whilst the STE ranged in the middle. However, the actual contact mechanism leads to the descending sequence of favorable friction behaviors nominally as： NP, INE and STE.
文摘In array signal processing, 2-D spatial-spectrum estimation is required to determine DOA of multiple signals. The circular array of sensors is found to possess several nice properties for DOA estimation of wide-band sources. C. U. Padmini, et al.(1994) had suggested that the frequency-direction ambiguity in azimuth estimation of wide-baud signals received by a uniform linear array (ULA) can be avoided by using a circular array, even without the use of any delay elements. In 2-D spatial-spectrum estimation for wide-band signals, the authors find that it is impossible to avoid the ambiguity in source frequency-elevation angle pairs using a circular array. In this paper, interpolated circular arrays are used to perform 2-D spatial-spectrum estimation for wide-band sources. In the estimation, a large aperture circular array (Υ】λmin/2) is found to possess superior resolution capability and robustness.
文摘This paper extends the Non-Circular MUltiple SIgnal Classification(MUSIC)(NC-MUSIC) method for the common array geometries including Uniform Circular Arrays(UCAs) and Uniform Rectangular Arrays(URAs),which enables the algorithm to estimate 2-D Direction Of Arrival(DOA).A comparison between UCAs and URAs of NC-MUSIC is made in this paper.The simulations show that the NC-MUSIC method doubles the maximum estimation number of standard MUSIC.Using non-circular signals,the performance of URAs is improved remarkably while the improvement of UCAs is not so significantly.Moreover,the influence of arrays structures on the NC-MUSIC method is discussed.
基金Jiangsu Provincial Maternal and Child Health Project Fund(Project No.:F202030)。
文摘Objective:To elucidate the regulatory effects and mechanisms of circular RNA MGAT5(circMGAT5)on the invasive ability of human ovarian cancer(OC)cells.Methods:Human OC SKOV-3 cells were transfected with circMGAT5 interference plasmids.The expression level of circMGAT5 was detected by quantitative real-time polymerase chain reaction(qRT-PCR).The proliferation and invasive abilities of cancer cells were determined using soft agar colony formation assay and Transwell assay,respectively.The expression of matrix metalloproteinase-2(MMP-2)at mRNA and protein levels was detected by qRT-PCR and immunofluorescence,respectively.Results:CircMGAT5 level in the circMGAT5-transfected group was significantly downregulated in a concentration-and time-dependent manner(p<0.005).Soft agar colony formation assay exhibited that the number of colonies formed in the 6.25,12.5,and 25.0 nM siRNA groups was significantly reduced in a concentration-dependent manner(p<0.005).Transwell assay revealed that the number of cells penetrating the filter membrane in the 6.25,12.5,and 25.0 nM siRNA groups was significantly decreased in a concentration-dependent manner(p<0.005).qRT-PCR and immunofluorescence results demonstrated that the mRNA and protein levels of MMP-2 in OC cells of the siRNA-transfected group were significantly decreased.Conclusion:circMGAT5 plays an important role in the invasion of OC cells,and its mechanism may be related to the downregulation of MMP-2 expression.
