A full-length cDNA library from the antenna of Chrysopa pallens （Rambur） was constructed based on switching mechanism at 5＇ end of RNA transcript （SMART） system. The purified double-stranded cDNA was ligated to v...A full-length cDNA library from the antenna of Chrysopa pallens （Rambur） was constructed based on switching mechanism at 5＇ end of RNA transcript （SMART） system. The purified double-stranded cDNA was ligated to vector SMART2IFD, and transformed into gscherichia coli DHSa by electroporation. The li-brary constructed for female and male reached high titers of 2.1 × 106^ and 1.8 × 106^ pfu/mL, respectively. PCR results showed that the inserts varied from 400 bp to 2 000 bp with the average size larger than 500 bp and the recombination rate over 93.0%. FuRher studies on those genes and large scale sequencing of the library may be helpful in screening new olfactory related genes in C. pallens.展开更多
[Objectives]The paper was to provide background information for ongoing research on relationship between smell and behavior of the green lacewing Chrysopa pallens（ Rambur）（ Neuroptera： Chrysopidae）. [Methods]The ...[Objectives]The paper was to provide background information for ongoing research on relationship between smell and behavior of the green lacewing Chrysopa pallens（ Rambur）（ Neuroptera： Chrysopidae）. [Methods]The submicroscopic structure of antennal sensilla of C. pallens was examined using scanning electron microscopy. [Results]Antennae of female and male C. pallens were linear in shape and（ 1. 52 ± 0. 08） and（ 1. 58 ± 0. 23） cm in length,respectively. The scape and pedicel were composed of single sub-segment,while the flagellum consisted of 116 sub-segments,on which most sensilla distributed. The flagella of female and male antennae were（ 1. 44 ± 0. 04） and（ 1. 47 ± 0. 13） cm in length,respectively. Nine morphological sensilla types were recorded in both sexes,including four types of highly abundant and widely distributed sensilla trichodea（ ST Ⅰ,ST Ⅱ,ST Ⅲ and ST Ⅳ）,three types of sensilla basiconica（ SB Ⅰ,SB Ⅱ,and SB Ⅲ）,and one of each type of sensilla chaetica and B9 hm bristles. Although the shape,structure,numbers,and distribution of antennae of females and males were basically similar,major differences were recorded between the sexes in the length of some sensilla types. Both the ST Ⅲ and SB Ⅰ in female adults were significantly longer than that in male ones. Notably,SB Ⅱ was found only in female. [Conclusions]The paper laid a foundation for revealing the relationship between smell and behavior,playing a crucial role in promoting the important biological control effect of C. pallens in farmland ecological system.展开更多
基金Supported by 973 Program(2013CB127602)Special Fund for Agro-scientific Research in the Public Interest(201103002)948 Project(2011-G4)
文摘A full-length cDNA library from the antenna of Chrysopa pallens （Rambur） was constructed based on switching mechanism at 5＇ end of RNA transcript （SMART） system. The purified double-stranded cDNA was ligated to vector SMART2IFD, and transformed into gscherichia coli DHSa by electroporation. The li-brary constructed for female and male reached high titers of 2.1 × 106^ and 1.8 × 106^ pfu/mL, respectively. PCR results showed that the inserts varied from 400 bp to 2 000 bp with the average size larger than 500 bp and the recombination rate over 93.0%. FuRher studies on those genes and large scale sequencing of the library may be helpful in screening new olfactory related genes in C. pallens.
基金Supported by Special Fund for Agro-scientific Research in the Public Interest(201103002)National "948" Program(2011-G4)
文摘[Objectives]The paper was to provide background information for ongoing research on relationship between smell and behavior of the green lacewing Chrysopa pallens（ Rambur）（ Neuroptera： Chrysopidae）. [Methods]The submicroscopic structure of antennal sensilla of C. pallens was examined using scanning electron microscopy. [Results]Antennae of female and male C. pallens were linear in shape and（ 1. 52 ± 0. 08） and（ 1. 58 ± 0. 23） cm in length,respectively. The scape and pedicel were composed of single sub-segment,while the flagellum consisted of 116 sub-segments,on which most sensilla distributed. The flagella of female and male antennae were（ 1. 44 ± 0. 04） and（ 1. 47 ± 0. 13） cm in length,respectively. Nine morphological sensilla types were recorded in both sexes,including four types of highly abundant and widely distributed sensilla trichodea（ ST Ⅰ,ST Ⅱ,ST Ⅲ and ST Ⅳ）,three types of sensilla basiconica（ SB Ⅰ,SB Ⅱ,and SB Ⅲ）,and one of each type of sensilla chaetica and B9 hm bristles. Although the shape,structure,numbers,and distribution of antennae of females and males were basically similar,major differences were recorded between the sexes in the length of some sensilla types. Both the ST Ⅲ and SB Ⅰ in female adults were significantly longer than that in male ones. Notably,SB Ⅱ was found only in female. [Conclusions]The paper laid a foundation for revealing the relationship between smell and behavior,playing a crucial role in promoting the important biological control effect of C. pallens in farmland ecological system.
