[Objective] This study was conducted to investigate the crossbreeding method for Chinese water chestnut. [Method] The growth of Chinese water chestnut stalk and inflorescence was observed. Chinese water chestnut flowe...[Objective] This study was conducted to investigate the crossbreeding method for Chinese water chestnut. [Method] The growth of Chinese water chestnut stalk and inflorescence was observed. Chinese water chestnut flowering law and stamen and pistil growth were investigated in an artificial pollination experiment. [Result] Stamens and pistils reached their full bloom stages when the flower stalk lengths were approximate 37.0 and 46.9 cm, respectively, with a height difference of 9.84 cm and time difference of 28.5 h; and 9：00-11：00 a.m. was the optimal period for artificial pollination. Seven hours after artificial pollination, pistils wilted rapidly. The seed setting rates under artificial pollination and a natural condition was 43% and 15.5%, respectively. [Conclusion} The time difference between the full bloom stages of stamens and pistils is crucial to the success of artificial pollination of Chinese water chestnut. The seed setting rate under artificial pollination is much higher than that under a natural condition.展开更多
With the forms of pistil stigma of " Guiti 2" Chinese water chestnut cultivar during the pollination as the object of observation,we reveal the pollination process of Chinese water chestnut from the microsco...With the forms of pistil stigma of " Guiti 2" Chinese water chestnut cultivar during the pollination as the object of observation,we reveal the pollination process of Chinese water chestnut from the microscopic point of view,to provide reference for Chinese water chestnut crossbreeding. The results show that the Chinese water chestnut pistil has 2- 4 stigmas which present white filament and vascular bundle forms,and the vessels on epidermis are thick with long translucent branched hairs; after pollination,pollen grains are tightly bound on branched hairs,and after identification,the pollen tube can penetrate branched hairs,continue to grow,and transfer the genetic material in pollen to vascular bundle.展开更多
Fresh-cut Chinese water chestnuts(CWCs)are prone to quality deterioration during storage,which does not meet consumer demand.In this study,the effect of exogenous melatonin(5 mmol·L^(-1))on the quality and potent...Fresh-cut Chinese water chestnuts(CWCs)are prone to quality deterioration during storage,which does not meet consumer demand.In this study,the effect of exogenous melatonin(5 mmol·L^(-1))on the quality and potential mechanisms in fresh-cut CWC was investigated.The results showed that melatonin treatment alleviated the cut-surface discoloration of CWCs.Not only did this treatment significantly slow down the in-crease in browning degree and yellowness(b*)as well as the decrease in lightness(L*),but it also significantly delayed the loss of weight and total soluble solids.Further investigations indicated that melatonin-treated fresh-cut CWCs exhibited significantly lower total phenolics and soluble quinones and suppressed the activities of phenylalanine ammonia-lyase,polyphenol oxidase,and peroxidase.Meanwhile,when fresh-cut CWCs were treated with melatonin,the total flavonoid concentration was significantly decreased compared to the control.Ad ditionally,melatonin significantly inhibited the accumulation of H_(2)O_(2)and malondialdehyde as well as enhanced the activities of superoxide dismutase and catalase by promoting the production of O_(2)^(-·).In summary,melatonin treatment may delay the surface discoloration of fresh-cut CWCs by inhibiting phenolic compound metabolism and improving antioxidant capacity,thereby effectively maintaining the quality and prolonging the shelf life of fresh-cut CWCs.展开更多
In this study,we applied widely targeted metabolomics and physiological analysis to investigate the non-volatile metabolites changes and regulatory mechanisms of high-pressure carbon dioxide(HPCD)treatment on the yell...In this study,we applied widely targeted metabolomics and physiological analysis to investigate the non-volatile metabolites changes and regulatory mechanisms of high-pressure carbon dioxide(HPCD)treatment on the yellowing of fresh-cut Chinese water chestnut(CWC).A total of 349 non-volatile metabolites were identified and subjected to principal component analysis(PCA),OPLS-DA and KEGG enrichment analysis.The results showed that the yellowing of fresh-cut CWC was related to membrane lipid metabolism and flavonoid biosynthesis.Damage stress induced membrane lipid metabolism and flavonoid biosynthesis in surface tissues,and the increase of phenylalanine ammonia-lyase(PAL)activity in flavonoid biosynthesis might be triggered by signaling molecules from membrane lipid metabolism.On the one hand,HPCD treatment could directly inhibit the activity of PAL,and reduce the biosynthesis of flavonoids in the damaged tissues.On the other hand,HPCD could indirectly inhibit the generation and accumulation of potential signaling molecules by regulating membrane lipid metabolism,thus preventing signaling molecules from triggering flavonoid biosynthesis.This study proposed a potential network of HPCD regulating the yellowing of fresh-cut CWC and established the foundation for further control of yellowing in fresh-cut CWC.展开更多
文摘[Objective] This study was conducted to investigate the crossbreeding method for Chinese water chestnut. [Method] The growth of Chinese water chestnut stalk and inflorescence was observed. Chinese water chestnut flowering law and stamen and pistil growth were investigated in an artificial pollination experiment. [Result] Stamens and pistils reached their full bloom stages when the flower stalk lengths were approximate 37.0 and 46.9 cm, respectively, with a height difference of 9.84 cm and time difference of 28.5 h; and 9：00-11：00 a.m. was the optimal period for artificial pollination. Seven hours after artificial pollination, pistils wilted rapidly. The seed setting rates under artificial pollination and a natural condition was 43% and 15.5%, respectively. [Conclusion} The time difference between the full bloom stages of stamens and pistils is crucial to the success of artificial pollination of Chinese water chestnut. The seed setting rate under artificial pollination is much higher than that under a natural condition.
文摘With the forms of pistil stigma of " Guiti 2" Chinese water chestnut cultivar during the pollination as the object of observation,we reveal the pollination process of Chinese water chestnut from the microscopic point of view,to provide reference for Chinese water chestnut crossbreeding. The results show that the Chinese water chestnut pistil has 2- 4 stigmas which present white filament and vascular bundle forms,and the vessels on epidermis are thick with long translucent branched hairs; after pollination,pollen grains are tightly bound on branched hairs,and after identification,the pollen tube can penetrate branched hairs,continue to grow,and transfer the genetic material in pollen to vascular bundle.
基金the National Natural Science Foundation of China(No.32001764)the Student Scientific Research Program of Wuhan Polytechnic University(No.xsky2021002),China.
文摘Fresh-cut Chinese water chestnuts(CWCs)are prone to quality deterioration during storage,which does not meet consumer demand.In this study,the effect of exogenous melatonin(5 mmol·L^(-1))on the quality and potential mechanisms in fresh-cut CWC was investigated.The results showed that melatonin treatment alleviated the cut-surface discoloration of CWCs.Not only did this treatment significantly slow down the in-crease in browning degree and yellowness(b*)as well as the decrease in lightness(L*),but it also significantly delayed the loss of weight and total soluble solids.Further investigations indicated that melatonin-treated fresh-cut CWCs exhibited significantly lower total phenolics and soluble quinones and suppressed the activities of phenylalanine ammonia-lyase,polyphenol oxidase,and peroxidase.Meanwhile,when fresh-cut CWCs were treated with melatonin,the total flavonoid concentration was significantly decreased compared to the control.Ad ditionally,melatonin significantly inhibited the accumulation of H_(2)O_(2)and malondialdehyde as well as enhanced the activities of superoxide dismutase and catalase by promoting the production of O_(2)^(-·).In summary,melatonin treatment may delay the surface discoloration of fresh-cut CWCs by inhibiting phenolic compound metabolism and improving antioxidant capacity,thereby effectively maintaining the quality and prolonging the shelf life of fresh-cut CWCs.
基金supported by the Fundamental Research Funds for the Central Universities(2662020SPPY012).
文摘In this study,we applied widely targeted metabolomics and physiological analysis to investigate the non-volatile metabolites changes and regulatory mechanisms of high-pressure carbon dioxide(HPCD)treatment on the yellowing of fresh-cut Chinese water chestnut(CWC).A total of 349 non-volatile metabolites were identified and subjected to principal component analysis(PCA),OPLS-DA and KEGG enrichment analysis.The results showed that the yellowing of fresh-cut CWC was related to membrane lipid metabolism and flavonoid biosynthesis.Damage stress induced membrane lipid metabolism and flavonoid biosynthesis in surface tissues,and the increase of phenylalanine ammonia-lyase(PAL)activity in flavonoid biosynthesis might be triggered by signaling molecules from membrane lipid metabolism.On the one hand,HPCD treatment could directly inhibit the activity of PAL,and reduce the biosynthesis of flavonoids in the damaged tissues.On the other hand,HPCD could indirectly inhibit the generation and accumulation of potential signaling molecules by regulating membrane lipid metabolism,thus preventing signaling molecules from triggering flavonoid biosynthesis.This study proposed a potential network of HPCD regulating the yellowing of fresh-cut CWC and established the foundation for further control of yellowing in fresh-cut CWC.
