AIM: To validate accuracy of a novel chair-side test for matrix metalloproteinase(MMP)-8 as compared to enzyme-linked immunosorbent assay(ELISA) in Periodontal health and disease.METHODS: Gingival crevicular fluid was...AIM: To validate accuracy of a novel chair-side test for matrix metalloproteinase(MMP)-8 as compared to enzyme-linked immunosorbent assay(ELISA) in Periodontal health and disease.METHODS: Gingival crevicular fluid was collected from 150 subjects, Group 1(healthy)- 50 subjects, Group 2(gingivitis)- 50 subjects and Group 3(chronic periodontitis)- 50 subjects. A chair-side test strip was indigenously prepared using polyclonal antibodies(principle of immunochromatography) to detect the MMP-8 levels. The detection accuracy(sensitivity and specificity) of the MMP-8 levels by chair-side test kit were compared with ELISA at baseline and 3 mo after scaling and root planing among the study population.RESULTS: The novel chair side test detected MMP-8 levels in accordance with ELISA which at baseline were higher in Group 2 and Group 3 as compared to controls(P < 0.05), and these enzyme levels decreased after therapy(P < 0.05). The chair-side test could differentiate healthy, gingivitis and periodontitis. The detection accuracy of the chair-side test strip were on par with ELISA(sensitivity 92.9% and specificity of 100%) which were statistically significant(P < 0.05). A desire to arouse interest about periodontal health and maintenance in the Indian population provided a strong rationale for us to develop our chair-side test strips to suit our economy. Moreover, this was the first ever effort to develop and validate a chair-side test strip to detect MMP-8 levels in the Indian population. This test can be used on a large scale in private dental practice for the early detection of disease, tapping the sites at risk for disease, alongside helps in patient education and motivation for maintenance.CONCLUSION: This study shows that the novel chair side test kit detects MMP-8 levels a biomarker of periodontal disease progression accurately making it a good chair side diagnostic tool. Further, it is cost effective and time saving which can make it applicable in private dental practice on a large scale for the early detection of periodontal disease.展开更多
Introduction: Several techniques have been described by which a pair of dentures may be replicated. Case Report: The method described in this paper used materials readily available in a private practice to generate a ...Introduction: Several techniques have been described by which a pair of dentures may be replicated. Case Report: The method described in this paper used materials readily available in a private practice to generate a pair of duplicate dentures for a 74-year-old Black male, which captured all of the features of the patients’ existing pair. These were modified chair-side and used to capture vital information on fit, occlusion and aesthetics, forming a template which was transferred to the laboratory and used to generate new dentures via the traditional laboratory process. The same template was also used to generate a pair of “AvaDent Digital Dentures” by digital scanning and production of a digital denture template for try and then fabrication of the digital denture. Conclusion: The results of this pilot study show that this simple chair-side method of fabricating duplicate dentures can be incorporated successfully into the AvaDent Digital Denture fabrication process to generate a pair of dentures with comparability if not superior fit and aesthetics to a pair fabricated from the same template via the traditional laboratory process.展开更多
This article focuses on the connection between Leibniz and the production of Saxony hard porcelain.Leibniz commissioned Jesuits to investigate China’s porcelain-making techniques,sharing relevant information with Zin...This article focuses on the connection between Leibniz and the production of Saxony hard porcelain.Leibniz commissioned Jesuits to investigate China’s porcelain-making techniques,sharing relevant information with Zinnhaus and inspiring him with scientific ideas.Zinnhaus achieved key technological breakthroughs,and Poterger continued to refine the formula and establish the factory.Although Leibniz did not directly participate in the experiments,he promoted this process through his ideas,information,and connections,serving as an important behind-the-scenes force.展开更多
In the early 18th century,to meet Europe's urgent demand for Jingdezhen's ceramic production techniques,French Jesuit missionary François Xavier d'Entrecolles traveled to Jingdezhen,Jiangxi.While cond...In the early 18th century,to meet Europe's urgent demand for Jingdezhen's ceramic production techniques,French Jesuit missionary François Xavier d'Entrecolles traveled to Jingdezhen,Jiangxi.While conducting missionary work,he thoroughly investigated local porcelain-making techniques.He compiled his findings into two lengthy letters and sent samples of porcelain raw materials to Europe,successfully disseminating Jingdezhen's ceramic artistry to the continent.D'Entrecolles skillfully integrated the protective function of his religious identity,the resource coordination capabilities of political networks,the documentation methods of empirical science,and a localized infiltration strategy,constructing a four-dimensional investigative framework of"missionary integration-observation and interviews-literature review-experimental verification"through Broussonetia papyrifera.These systematic research methods enabled him to comprehensively master Jingdezhen's porcelain technology at the time,establishing him as the pioneer of Western systematic studies on Chinese ceramics and creating a new paradigm of"field research+scientific empiricism"in early cross-cultural technological transmission.展开更多
文摘AIM: To validate accuracy of a novel chair-side test for matrix metalloproteinase(MMP)-8 as compared to enzyme-linked immunosorbent assay(ELISA) in Periodontal health and disease.METHODS: Gingival crevicular fluid was collected from 150 subjects, Group 1(healthy)- 50 subjects, Group 2(gingivitis)- 50 subjects and Group 3(chronic periodontitis)- 50 subjects. A chair-side test strip was indigenously prepared using polyclonal antibodies(principle of immunochromatography) to detect the MMP-8 levels. The detection accuracy(sensitivity and specificity) of the MMP-8 levels by chair-side test kit were compared with ELISA at baseline and 3 mo after scaling and root planing among the study population.RESULTS: The novel chair side test detected MMP-8 levels in accordance with ELISA which at baseline were higher in Group 2 and Group 3 as compared to controls(P < 0.05), and these enzyme levels decreased after therapy(P < 0.05). The chair-side test could differentiate healthy, gingivitis and periodontitis. The detection accuracy of the chair-side test strip were on par with ELISA(sensitivity 92.9% and specificity of 100%) which were statistically significant(P < 0.05). A desire to arouse interest about periodontal health and maintenance in the Indian population provided a strong rationale for us to develop our chair-side test strips to suit our economy. Moreover, this was the first ever effort to develop and validate a chair-side test strip to detect MMP-8 levels in the Indian population. This test can be used on a large scale in private dental practice for the early detection of disease, tapping the sites at risk for disease, alongside helps in patient education and motivation for maintenance.CONCLUSION: This study shows that the novel chair side test kit detects MMP-8 levels a biomarker of periodontal disease progression accurately making it a good chair side diagnostic tool. Further, it is cost effective and time saving which can make it applicable in private dental practice on a large scale for the early detection of periodontal disease.
文摘Introduction: Several techniques have been described by which a pair of dentures may be replicated. Case Report: The method described in this paper used materials readily available in a private practice to generate a pair of duplicate dentures for a 74-year-old Black male, which captured all of the features of the patients’ existing pair. These were modified chair-side and used to capture vital information on fit, occlusion and aesthetics, forming a template which was transferred to the laboratory and used to generate new dentures via the traditional laboratory process. The same template was also used to generate a pair of “AvaDent Digital Dentures” by digital scanning and production of a digital denture template for try and then fabrication of the digital denture. Conclusion: The results of this pilot study show that this simple chair-side method of fabricating duplicate dentures can be incorporated successfully into the AvaDent Digital Denture fabrication process to generate a pair of dentures with comparability if not superior fit and aesthetics to a pair fabricated from the same template via the traditional laboratory process.
文摘This article focuses on the connection between Leibniz and the production of Saxony hard porcelain.Leibniz commissioned Jesuits to investigate China’s porcelain-making techniques,sharing relevant information with Zinnhaus and inspiring him with scientific ideas.Zinnhaus achieved key technological breakthroughs,and Poterger continued to refine the formula and establish the factory.Although Leibniz did not directly participate in the experiments,he promoted this process through his ideas,information,and connections,serving as an important behind-the-scenes force.
文摘In the early 18th century,to meet Europe's urgent demand for Jingdezhen's ceramic production techniques,French Jesuit missionary François Xavier d'Entrecolles traveled to Jingdezhen,Jiangxi.While conducting missionary work,he thoroughly investigated local porcelain-making techniques.He compiled his findings into two lengthy letters and sent samples of porcelain raw materials to Europe,successfully disseminating Jingdezhen's ceramic artistry to the continent.D'Entrecolles skillfully integrated the protective function of his religious identity,the resource coordination capabilities of political networks,the documentation methods of empirical science,and a localized infiltration strategy,constructing a four-dimensional investigative framework of"missionary integration-observation and interviews-literature review-experimental verification"through Broussonetia papyrifera.These systematic research methods enabled him to comprehensively master Jingdezhen's porcelain technology at the time,establishing him as the pioneer of Western systematic studies on Chinese ceramics and creating a new paradigm of"field research+scientific empiricism"in early cross-cultural technological transmission.
