Objective To establish an analytical method for the simultaneous determination of celosin Ⅰ and celosin Ⅱ, two major triterpenoids in Celosia Semen and compare the contents of celosin Ⅰ and celosin Ⅱ from differen...Objective To establish an analytical method for the simultaneous determination of celosin Ⅰ and celosin Ⅱ, two major triterpenoids in Celosia Semen and compare the contents of celosin Ⅰ and celosin Ⅱ from different habitats to screen the resources of elite germplasm for further applications. Methods A sensitive and simple high- performance liquid chromatography coupled with evaporative light-scattering detector（HPLC-ELSD） method was developed for the first time for the simultaneous determination of celosin Ⅰ and celosin Ⅱ. Using this method, 21 batches of Celosiae Semen were determined from different habitats in China. Results There was an obvious difference in the contents of celosin Ⅰ and celosin Ⅱ among Celosiae Semen species from various habitats across China. The crude drug from Yongzhou, Hunan province, Zhuhai, Guangdong province, and Nanning, Guangxi province showed the highest contents of all habitats, while Anguo, Hebei province, Haidian, Beijing, and Zhengzhou, Henan province showed the lowest content. The results also showed that geographical location had a great influence on the contents of the two compounds. The batches from lower latitudes were higher in contents of celosin Ⅰ and celosin Ⅱ. Conclusion The sun light may be a key factor influencing the contents of the two saponins, indicating that the environment has a great impact on the quality of Celosiae Semen.展开更多
Two cyclopeptides, celogentin L(1) and its epimer lyciumin A(2) were firstly isolated from Celosia argentea L.. The planar structures of the two compounds were fully determined by spectroscopic data, including 1 D-, 2...Two cyclopeptides, celogentin L(1) and its epimer lyciumin A(2) were firstly isolated from Celosia argentea L.. The planar structures of the two compounds were fully determined by spectroscopic data, including 1 D-, 2 D-NMR, and HR-ESI/MS. The absolute configurations of amino acid components were assigned via chiral-phase HPLC analyses after acid hydrolysis. Furthermore, the configuration of C-N linkage at the glycine Cα was elucidated by extensive analyses of 2 D-NMR and comparison of the experimental and calculated electronic circular dichroism(ECD) spectra. Cytotoxicity of the two compounds against human alveolar epithelial A549, hepatocellular carcinoma Hep G2, and cervical cancer Hela cell lines was assayed. Although both of them were inactive in these cells, the present findings add new facets for the chemistry of Celosia argentea.展开更多
Productivity responses using organic approaches will assist to elucidate crop responses under different intercrop population levels. Experiments were conducted to determine the growth, yield and productivity responses...Productivity responses using organic approaches will assist to elucidate crop responses under different intercrop population levels. Experiments were conducted to determine the growth, yield and productivity responses of component crops in snake tomato/celosia mixtures to intercrop population and organic manure application rates. The 4 × 4 factorial experiment arranged in completely randomized design (CRD) was replicated three times. The snake tomato using potting media was cultivated in 2013 and 2014 at 100,000 plants/ha (one plant/pot) alongside intercrop celosia at three population levels of 100,000 (P1), 200,000 (P2) and 300,000 plant/ha (P3) (10, 20 and 30 plants/pot respectively) where sole crops (P0) served as control. The crop mixture was supplied with poultry manure (PM) at 5 (F1), 10 (F2) and 20 t/ha (F3) using the unfertilized (0 t/ha-F0) as control. The result showed that plants in “year II” had longer vines, thicker girth and more side-vines while those in “year I” had more leaves, more male/female flowers, longer and thicker fruit sizes and heavier fruit yield. Intercropping with celosia at P1 produced plants with thicker girth and more leaves, plants at P1 - P3 had more side-vines while all intercropped plots had more female flowers, but P1 had thicker fruit girth compared to sole crops. Plants with F3 had longer vine length, more leaves and more number of side-vines, more female flowers, longer fruits, thicker fruit girth and heavier fruit yield, but those with F1 or F3 had thicker girth compared to other rates while those with F2 had better productivity advantage with LER and ATER at >1.0. In conclusion, snake tomato and celosia in crop mixture were best with F2 and F3 respectively, although intercropping retarded growth and yield of the component crops but P1 was comparable with sole.展开更多
The efficacy of crude extracts of Senna alata in the improvement of vegetative and reproductive growth in Celosia aregentea was investigated. Fresh leaves of S. alata were blended with a homogenizer in 1 litre of dist...The efficacy of crude extracts of Senna alata in the improvement of vegetative and reproductive growth in Celosia aregentea was investigated. Fresh leaves of S. alata were blended with a homogenizer in 1 litre of distilled water. The resultant green paste was filtered under suction. Different concentrations (75%, 50%, 40%, 30%, 25%, 12%, 10%, and 5%) were prepared from the 100% crude extract. Seeds of C. argentea were presoaked in these different concentrations including a control (0%) and planted out after 24 hours. Results obtained showed that seedling height, leaf area, dry weight and leaf area ratio were promoted and enhanced by presoaking seeds in the extract. At the end of the experimental period (six weeks), seedling height in 75% and 100% treatments were 109 ± 16.12 cm and 117 ± 19.32 cm, leaf area 128 ± 17.91 cm2 and 125 ± 18.12 cm2, dry weight 7.48 kg and 7.0 kg respectively. Seedlings raised from seeds presoaked in water (control) however, flowered earlier (8 weeks) than the treatments (10 weeks in 75% and 100%). Presoaking seeds of C. argentea in crude extracts of S. alata before planting is recommended for optimum production of the leafy vegetable. The procedure is cheap and easily implementable by resource-poor farmers who are the main growers of C. argentea.展开更多
Celosia L. is richly distributed in China and there are about 60 species in the world. The chemical constituents of Celosia L. include flavonoids, triterpenoid saponins, steroids, organic acids, and cyclic peptides. W...Celosia L. is richly distributed in China and there are about 60 species in the world. The chemical constituents of Celosia L. include flavonoids, triterpenoid saponins, steroids, organic acids, and cyclic peptides. Which have a variety of effects such as antimicrobial, hepatoprotective and antitumour. To provide a scientific foundation for the subsequent phase of research and development for Celosia L., 30 years of pertinent global literature is summarized, analyzed, and categorized. An overview of 75 chemical constituents, pharmacological activities, and clinical applications is provided.展开更多
基金Science and Technology Commission Research Projects of Shanghai Municipality(11DZ1970501)
文摘Objective To establish an analytical method for the simultaneous determination of celosin Ⅰ and celosin Ⅱ, two major triterpenoids in Celosia Semen and compare the contents of celosin Ⅰ and celosin Ⅱ from different habitats to screen the resources of elite germplasm for further applications. Methods A sensitive and simple high- performance liquid chromatography coupled with evaporative light-scattering detector（HPLC-ELSD） method was developed for the first time for the simultaneous determination of celosin Ⅰ and celosin Ⅱ. Using this method, 21 batches of Celosiae Semen were determined from different habitats in China. Results There was an obvious difference in the contents of celosin Ⅰ and celosin Ⅱ among Celosiae Semen species from various habitats across China. The crude drug from Yongzhou, Hunan province, Zhuhai, Guangdong province, and Nanning, Guangxi province showed the highest contents of all habitats, while Anguo, Hebei province, Haidian, Beijing, and Zhengzhou, Henan province showed the lowest content. The results also showed that geographical location had a great influence on the contents of the two compounds. The batches from lower latitudes were higher in contents of celosin Ⅰ and celosin Ⅱ. Conclusion The sun light may be a key factor influencing the contents of the two saponins, indicating that the environment has a great impact on the quality of Celosiae Semen.
基金financially supported by the National Natural Science Foundation of China(Nos.81322051 and 31400300)the Natural Science Foundation of Jiangsu Province(No.BK20151442)the Project Funded by the Priority Academic Program Development(PAPD) of Jiangsu Higher Education Institutions
文摘Two cyclopeptides, celogentin L(1) and its epimer lyciumin A(2) were firstly isolated from Celosia argentea L.. The planar structures of the two compounds were fully determined by spectroscopic data, including 1 D-, 2 D-NMR, and HR-ESI/MS. The absolute configurations of amino acid components were assigned via chiral-phase HPLC analyses after acid hydrolysis. Furthermore, the configuration of C-N linkage at the glycine Cα was elucidated by extensive analyses of 2 D-NMR and comparison of the experimental and calculated electronic circular dichroism(ECD) spectra. Cytotoxicity of the two compounds against human alveolar epithelial A549, hepatocellular carcinoma Hep G2, and cervical cancer Hela cell lines was assayed. Although both of them were inactive in these cells, the present findings add new facets for the chemistry of Celosia argentea.
文摘Productivity responses using organic approaches will assist to elucidate crop responses under different intercrop population levels. Experiments were conducted to determine the growth, yield and productivity responses of component crops in snake tomato/celosia mixtures to intercrop population and organic manure application rates. The 4 × 4 factorial experiment arranged in completely randomized design (CRD) was replicated three times. The snake tomato using potting media was cultivated in 2013 and 2014 at 100,000 plants/ha (one plant/pot) alongside intercrop celosia at three population levels of 100,000 (P1), 200,000 (P2) and 300,000 plant/ha (P3) (10, 20 and 30 plants/pot respectively) where sole crops (P0) served as control. The crop mixture was supplied with poultry manure (PM) at 5 (F1), 10 (F2) and 20 t/ha (F3) using the unfertilized (0 t/ha-F0) as control. The result showed that plants in “year II” had longer vines, thicker girth and more side-vines while those in “year I” had more leaves, more male/female flowers, longer and thicker fruit sizes and heavier fruit yield. Intercropping with celosia at P1 produced plants with thicker girth and more leaves, plants at P1 - P3 had more side-vines while all intercropped plots had more female flowers, but P1 had thicker fruit girth compared to sole crops. Plants with F3 had longer vine length, more leaves and more number of side-vines, more female flowers, longer fruits, thicker fruit girth and heavier fruit yield, but those with F1 or F3 had thicker girth compared to other rates while those with F2 had better productivity advantage with LER and ATER at >1.0. In conclusion, snake tomato and celosia in crop mixture were best with F2 and F3 respectively, although intercropping retarded growth and yield of the component crops but P1 was comparable with sole.
文摘The efficacy of crude extracts of Senna alata in the improvement of vegetative and reproductive growth in Celosia aregentea was investigated. Fresh leaves of S. alata were blended with a homogenizer in 1 litre of distilled water. The resultant green paste was filtered under suction. Different concentrations (75%, 50%, 40%, 30%, 25%, 12%, 10%, and 5%) were prepared from the 100% crude extract. Seeds of C. argentea were presoaked in these different concentrations including a control (0%) and planted out after 24 hours. Results obtained showed that seedling height, leaf area, dry weight and leaf area ratio were promoted and enhanced by presoaking seeds in the extract. At the end of the experimental period (six weeks), seedling height in 75% and 100% treatments were 109 ± 16.12 cm and 117 ± 19.32 cm, leaf area 128 ± 17.91 cm2 and 125 ± 18.12 cm2, dry weight 7.48 kg and 7.0 kg respectively. Seedlings raised from seeds presoaked in water (control) however, flowered earlier (8 weeks) than the treatments (10 weeks in 75% and 100%). Presoaking seeds of C. argentea in crude extracts of S. alata before planting is recommended for optimum production of the leafy vegetable. The procedure is cheap and easily implementable by resource-poor farmers who are the main growers of C. argentea.
文摘Celosia L. is richly distributed in China and there are about 60 species in the world. The chemical constituents of Celosia L. include flavonoids, triterpenoid saponins, steroids, organic acids, and cyclic peptides. Which have a variety of effects such as antimicrobial, hepatoprotective and antitumour. To provide a scientific foundation for the subsequent phase of research and development for Celosia L., 30 years of pertinent global literature is summarized, analyzed, and categorized. An overview of 75 chemical constituents, pharmacological activities, and clinical applications is provided.
