Background:Sargentodoxae Caulis(SC)is the vine stem of Sargentodoxa Cuneata(Oliv.)Rehd.&E.H.Wilson in C.S.Sargent,and it in traditional Chinese medicine has been known for promoting blood circulation and removing ...Background:Sargentodoxae Caulis(SC)is the vine stem of Sargentodoxa Cuneata(Oliv.)Rehd.&E.H.Wilson in C.S.Sargent,and it in traditional Chinese medicine has been known for promoting blood circulation and removing blood stasis as recorded in the ancient book“Illustrated Classics of Materia Medica”.It has been used effectively to treat blood stasis too in modern clinical practice.However,the anti-hyperlipidemia effect of SC is not fully understood.This paper aims at exploring the use of SC stems to improve the balance of blood lipids in the body,and its new role in treating hyperlipidemia.Methods:The effects of SC extract on hyperlipidemia were explored by combining lipidomics and gut microbiota.Secondly,we explored the potential mechanism of SC in treating hyperlipidemia by pathway analysis.Results:The results showed that the stem extract of SC could restore the physiological and biochemical indices of hyperlipidemia in mice,as well as repair the morphological and structural damage to tissues.Compared to the Model group,the SC extract significantly reduced the liver index,epididymal fat index,and Lee’s index.It also significantly decreased serum levels of total cholesterol,triglycerides,low-density lipoprotein cholesterol,peroxisome proliferator-activated receptor gamma(PPAR-γ),D-lactate,and free fatty acids,while significantly increasing the relative content of peroxisome proliferator-activated receptor alpha(PPAR-α).These changes were statistically significant.Non-targeted lipidomics,based on LC-MS,were utilized to investigate the lipid metabolism characteristics in serum,liver,and epididymal fat of the subjects.It was observed that,compared to the blank group,the Model group exhibited significant changes primarily in glycerol lipids and glycerophospholipids.The treatment group also displayed alterations in these lipids.A total of 38,81,and 27 differential lipids were identified in serum,liver,and epididymal fat samples,respectively.Among these,14 common differential lipids were found in both serum and liver samples,and their KEGG enrichment pathways were largely consistent.Among them,the sphingolipid signaling pathway and the glycerophospholipid metabolic pathway were identified as key metabolic pathways that were regulated.Our gut microbiota analysis revealed that SC diminishes the abundance of Actinobacteria by altering the cecal flora in mice.Conclusion:This alteration leads to the downregulation of genes involved in triglyceride metabolism,which in turn changes lipid processing and reduces triglyceride levels.Consequently,SC effectively combats hyperlipidemia.Notably,SC impacts key metabolic pathways,including the sphingolipid signaling and glycerophospholipid metabolism.These findings underscore SC’s therapeutic potential,positioning it as a promising alternative for reducing the health risks associated with hyperlipidemia.展开更多
Background:Triple-negative breast cancer(TNBC)has long been a difficult therapeutic target to overcome because of its extreme aggressiveness,poor efficacy and rarity of available treatment regimens.To explore the anti...Background:Triple-negative breast cancer(TNBC)has long been a difficult therapeutic target to overcome because of its extreme aggressiveness,poor efficacy and rarity of available treatment regimens.To explore the anti-TNBC potential of Caulis Polygoni Multiflori(CPM),this investigation applied an integrated computational and experimental approach.Methods:Bioactive CPM compounds were investigated via screening of three TCM databases(TCMSP,TCMID,and HERB)using Bioavailability(OB≥30%)as inclusion criteria.TNBC targets were obtained from the online resources GeneCards,CTD,and OMIM network pharmacology analysis was used to determine common target(s),utilizing two PPI analysis tools–STRING and Cytoscape.Molecular docking assessed target-compound interactions by means of AutoDock Vina.In vitro studies conducted on MDA-MB-231 cells involved evaluating inhibitory effects via MTT,wound-healing assay,transwell,and qPCR techniques.Results:Six bioactive compounds were identified,with emodin(IC_(50) approximately 15μM)and chrysophanol(IC50 approximately 25μM)showing strong binding to TNBC targets(emodin-HSP90AB1:−9.42 kcal/mol;chrysophanol-SRC:−8.32 kcal/mol).Network analysis revealed 143 shared targets,with BCL2,EGFR and ESR1 as key nodes.Pathway analysis implicated PI3K-AKT signaling and apoptosis.In vitro,emodin and chrysophanol synergistically inhibited proliferation,migration and invasion,while downregulating BCL2,EGFR and ESR1 expression.Conclusion:To the best of our knowledge,this is the first report that describes the synergistic mechanisms of multiple compounds from the extract of CPM in TNBC with chrysophanol and emodin working as the main components by mediating oncogenic pathways.展开更多
Objective:By using the method of network pharmacology to explore the mechanism of action of Caulis Sinomenii-Caulis Piperis Kadsurae herb pair in the treatment of rheumatoid arthritis.Methods:Search and screen the act...Objective:By using the method of network pharmacology to explore the mechanism of action of Caulis Sinomenii-Caulis Piperis Kadsurae herb pair in the treatment of rheumatoid arthritis.Methods:Search and screen the active ingredients and targets of Qingfengteng(Caulis Sinomenii)and Haifengteng(Caulis Piperis Kadsurae)through the TCMSP database,and predict and supplement the target points of the active ingredients through the Swiss Target Prediction platform;collect rheumatoid arthritis disease targets through GeneCards,OMIM database,and DRUGBANK database,construct a Venn diagram,and obtain the key target of Caulis Sinomenii-Caulis Piperis Kadsurae herb pairs in the treatment of rheumatoid arthritis;use Cytoscape 3.7.1 to establish the effect of Caysaceae sylvestris and Rhizoma sibirica in the treatment of rheumatoid arthritis"Drug-active ingredient-target"network diagram,submit key targets to STRING11.0 database to construct a protein interaction network model to obtain core targets;perform the GO and KEGG enrichment analysis of key targets on the Metascape platform,and draw the network diagram of"active pharmaceutical ingredients-targets-pathways".Results:After screening,discover a total of 27 active ingredients of Caulis Sinomenii-Caulis Piperis Kadsurae herb pairs,which can act on 229 targets,1269 related targets for rheumatoid arthritis diseases.And obtain a total of 95 common targets for drug active ingredients and diseases,including PTGS2,EGFR,MMP9,CASP3,JUN,etc.The common targets are mainly enriched in signaling pathways such as Pathways in cancer,Th17 cell differentiation and Endocrine resistance etc.Conclusion:This study preliminarily explored the potential mechanism of Caulis Sinomenii-Caulis Piperis Kadsurae herb pairs in the treatment of rheumatoid arthritis.The Caulis Sinomenii-Caulis Piperis Kadsurae herb pair may regulate cancer-related pathways,Th17 cell differentiation,endocrine resistance and other pathways to treat rheumatoid arthritis by regulating targets such as PTGS2,EGFR,and MMP9,providing clues and ideas for follow-up experimental research.展开更多
Matrix metalloproteinases（MMPs） play an essential role in development and tissue remodeling of living organisms. However, the overexpression of MMPs has lead to a series of diseases, such as cancer, arthritis, and a...Matrix metalloproteinases（MMPs） play an essential role in development and tissue remodeling of living organisms. However, the overexpression of MMPs has lead to a series of diseases, such as cancer, arthritis, and atherosclerosis; and inhibition of MMPs may have therapeutic benefits. The discovery of MMP inhibitors from herbal has become a prospective event. We showed that the extract of Polygoni multiflori caulis from ethyl acetate or water（ethyl acetate extract and water extract） can inhibit the activities of MMPs 9, 14, and 16 in a dose-dependent manner and n-butyl alcohol extract of it can also inhibit these MMPs. Furthermore, we found that n-butyl alcohol extract and water extract of it influence the cell viability. These discoveries may contribute to the development of MMP in- hibitors for the therapy of a variety of pathological conditions.展开更多
Heteroclitin D （H.D） was successfully isolated from Kadsurae Caulis by using flash chromatography and recrystalllzed by methanol, 10.2 mg of H.D was obtained from 4.86 g of crude extract, and the purity determined b...Heteroclitin D （H.D） was successfully isolated from Kadsurae Caulis by using flash chromatography and recrystalllzed by methanol, 10.2 mg of H.D was obtained from 4.86 g of crude extract, and the purity determined by HPLC was 99.4%. The structure was identified by UV, IR, MS, and NMR analysis. The fast, simple and efficient method can be applied to the preparation of reference substance of H. D.展开更多
There have appeared more and more antibiotics to antagonize against causative organism, but in comparison few antivirals have. Hence, many viral diseases remain refractory and fatal due to lack of effective medicine.C...There have appeared more and more antibiotics to antagonize against causative organism, but in comparison few antivirals have. Hence, many viral diseases remain refractory and fatal due to lack of effective medicine.Caulis Tripterygii wilfordii (TW) is a specific and potent Chinese medicine, and its effect in treating various intractable diseases, such as systemic lupus erythematosus, dermatomyositis, scleroderma, psoriasis and eczema has been confirmed by many medical researches. Pharmacological researches also proved that TW has anti-inflammatory and immunomodulatory actions. It is interesting that some effective components isolated from TW have an antiviral effect and more and more intention has been paid to related achievements at home and abroad.展开更多
Objective To study the drug effects of ＂Caulis Sargentodoxae Formula＂ which dears away heat and blood clot on experimental endometriosis in rat, and to compare this effect with Danazol＇s effects. Method The model o...Objective To study the drug effects of ＂Caulis Sargentodoxae Formula＂ which dears away heat and blood clot on experimental endometriosis in rat, and to compare this effect with Danazol＇s effects. Method The model of endometriosis rat was induced by transplanting with endometrium surgically The rats were divided into 6 groups randomly： the non-treatment group, the castrate group, Danazol group（80 mg/kg）, ＂Caulis Sargentodoxae Formula＂ group （110 g/kg）, the medium dose group（77.5 g/kg） and the low dose group（55 g/kg）. After 3 weeks＇ treatment, the volume ofendometrium was detected by cutting the rats＇ belly open, drawing the materials from endometrium for tissue section, and doing the quantity analysis of endometrium by semiautomatic image analysis machine.Results There was no significant difference of the endometrium volumes between the low dose group and the non-treatment group（P〉O.05）, other groups＇ endometrium volumes were significantly lower than those of the non-treatment group（P〈0. 05）. And apart from the low dose group, all other groups＇ heights of the endometrium epithelia were significantly lower than that of the non-treatment group（p〈0.01）. Conclusion ＂Caulis Sargentodoxae Formula＂ can refrain the growth of the endometrium, and the effects are better than that of Danazol＇s.展开更多
This study was conducted to investigate the changes of polysaccharides content in Dendrobii Officinalis Caulis and its pharmacological action. The results showed that different growth years, different periods, differe...This study was conducted to investigate the changes of polysaccharides content in Dendrobii Officinalis Caulis and its pharmacological action. The results showed that different growth years, different periods, different habitats and different external factors had influences on the change of polysaccharide content in Den- drobii Officinalis Caulis. Dendrobii Officinalis Caulis has certain effects of enhancing immunity, resisting fatigue and oxidation, promoting digestion and salivation secretion, reducing blood sugar and blood pressure, resisting tumor and liver injury, and relaxing bowels. Dendrobii Officinalis Caulis polysaccharides are the main effective components in Dendrobii Officinalis Caulis. The control of Dendrobii Officinalis Caulis polysaccharide content is of great significance to the assurance of the yield and quality of Dendrobii Officinalis Caulis material.展开更多
[Objectives]To establish the quality standard for Euonymi Grandiflorus Caulis and Folium.[Methods]Qualitative identification was performed by methods of origin identification,microscopic identification and thin layer ...[Objectives]To establish the quality standard for Euonymi Grandiflorus Caulis and Folium.[Methods]Qualitative identification was performed by methods of origin identification,microscopic identification and thin layer chromatography(TLC);the moisture,total ash,acid-insoluble ash,and extract were detected using the method in the Chinese Pharmacopoeia(2020 Edition),and the contents of quercetin and kaempferol were determined by High Performance Liquid Chromatography(HPLC).[Results]The leaves of Euonymi Grandiflorus were subleathery,narrow and long elliptic or narrow obovate with cyme,and capsules were often with narrow wing ridge.Characteristics of microscopic identification were significant.Calcium oxalate cluster crystals,cubic crystals,inlaid parenchyma cells,starch granules,stomata,and fiber bundles could be observed.A TLC method for identification of Euonymi Grandiflorus Caulis and Folium was established.The contents of water,total ash,acid-insoluble ash were not higher than 13%,9%,12%,respectively;the extract were no less were not higher than 13%,9%,12%and 17%in ten batches of samples,respectively;the sum of quercetin and kaempferol were no less than 0.20%.[Conclusions]The quality standard of Euonymi Grandiflorus Caulis and Folium were established through the research.This method is accurate,specific,and reproducible and can be used for the quality control of Euonymi Grandiflorus Caulis and Folium.展开更多
[Objectives]To explore the anti-tumor activity of the extracts of petroleum ether,ethyl acetate,n-butanol and aqueous solution from Mahoniae caulis.[Methods]The extracts were extracted with petroleum ether,ethyl aceta...[Objectives]To explore the anti-tumor activity of the extracts of petroleum ether,ethyl acetate,n-butanol and aqueous solution from Mahoniae caulis.[Methods]The extracts were extracted with petroleum ether,ethyl acetate,n-butanol and aqueous solution respectively,and then concentrated.The inhibitory effects of these extracts on the growth of three tumor cell lines in vitro were detected by CCK-8 method,and the IC 50 value was calculated.[Results]The four extracts inhibited the growth of the three tumor cell lines in vitro,among which the n-butanol extract had the best anti-tumor activity.The IC 50 values of the n-butanol extract on human gastric cancer(SGC-7901),human breast cancer(MCF-7)and human liver cancer(BEL-7404)cell lines were 0.23,0.25 and 0.58 mg/mL,respectively.[Conclusions]The ethanol extract of Mahoniae caulis under petroleum ether,ethyl acetate,n-butanol and aqueous solution had certain anti-tumor effect,and n-butanol extract had the best anti-tumor activity.展开更多
Mutong is a kind of medicine for promoting water circulation and drenching.There are many kinds of Aristolochia manshuriensis(Guan Mu Tong),which are commonly used in clinic,such as Mutong,Caulis Clematidis Armandii(C...Mutong is a kind of medicine for promoting water circulation and drenching.There are many kinds of Aristolochia manshuriensis(Guan Mu Tong),which are commonly used in clinic,such as Mutong,Caulis Clematidis Armandii(Chuan Mu Tong)and Mutong.After the event of"Longdan Xiegan pill",the doctor made Chuanmu general taboo,or replaced it with other drugs,or did not dare to use it for a long time,which affected the clinical effect.This paper discusses the toxicity of Mutong.展开更多
Objective:To study the effect of retention enema by caulis sargentodoxae enema combined with oral drugs on the immune inflammatory response and intestinal flora of UC patients. Methods: A total of 128 UC patients who ...Objective:To study the effect of retention enema by caulis sargentodoxae enema combined with oral drugs on the immune inflammatory response and intestinal flora of UC patients. Methods: A total of 128 UC patients who received treatment in the hospital between January 2013 and April 2017 were collected and divided into control group and observation group by random number table. Control group received conventional oral drug therapy, and the observation group received retention enema by caulis sargentodoxae enema combined with oral drug therapy. Before treatment and after 4 weeks of treatment, the differences in the levels of immunoglobulin and inflammatory response indexes in serum as well as intestinal flora levels in mucosa lesion tissue were compared between the two groups.Results: The differences in levels of immunoglobulin and inflammatory response indexes in serum as well as intestinal flora levels in mucosa lesion tissue were not statistically significant between the two groups of patients before treatment. After 4 weeks of treatment, IgA, IgG, IgM and IL-4 contents in serum as well as bifidobacterium and lactobacillus levels in mucosa lesion tissue of observation group were higher than those of control group while ESR, CRP, IL-6 and TNF-αcontents as well as E. coli level in mucosa lesion tissue were lower than those of control group. Conclusion: Retention enema by caulis sargentodoxae enema combined with oral drugs can effectively optimize the immune inflammatory state and balance the intestinal flora distribution in patients with UC.展开更多
Background:Trachelospermi Caulis et Folium,the stems and leaves of Trachelospermum jasminoides(Lindl.)Lem,has been used as a remedy for rheumatism,gonarthritis,backache and pharyngitis,according to the Chinese Pharmac...Background:Trachelospermi Caulis et Folium,the stems and leaves of Trachelospermum jasminoides(Lindl.)Lem,has been used as a remedy for rheumatism,gonarthritis,backache and pharyngitis,according to the Chinese Pharmacopoeia.However,the corresponding chemical substances of this ethnic medicinal plant with anti-inflammatory activity kept unclear.This study aimed to explore the active substances attribute to its anti-rheumatic activity of this medical plant.Methods:Ultra-high performance liquid chromatography combined with quadrupole time-of-flight mass spectrometry was used to identify the chemical components in the extract of T.jasminoides.Molecular docking was applied to analyze the binding affinity of potential active lignans to inducible nitric oxide synthase enzyme.The anti-inflammatory effects of the potential active fraction were verified by cell experiments in vitro.Results:Forty-one compounds,mainly are lignans and nine of which were reported for the first time from the genus of Trachelospermum,were characterized using ultra-high performance liquid chromatography quadrupole-time-of-flight mass spectrometry method.Molecular docking results showed these identified lignans had excellent interactions with the inducible nitric oxide synthase enzyme,and the lignans-enriched fraction could significantly inhibit the release of nitric oxide and the expression of inducible nitric oxide synthase protein in LPS-induced RAW264.7 cells.Conclusion:Lignans are the main active substances that responsible for the anti-inflammatory activity of the ethnic herbal medicine T.jasminoides.展开更多
A new dibenzylbutyrolactone lignan named matairesinol 4'-O-β-D-cellobioside(1),together with a known compound(7R,85)-dihydrodehydrodiconiferyl alcohol(2)was isolated from Caulis Trachelospermi.The extract of Caul...A new dibenzylbutyrolactone lignan named matairesinol 4'-O-β-D-cellobioside(1),together with a known compound(7R,85)-dihydrodehydrodiconiferyl alcohol(2)was isolated from Caulis Trachelospermi.The extract of Caulis Trachelospermi,which was separated by 80% alcohol extraction and subsequent HP-20 macroporous resin column chromatography,and its main components trachelogenin(3),nortrachelogenin(4),matairesinol(5)showed moderate inhibiting activities on NF-κB signaling pathway induced by TNFα,with the IC_(50) values of 17.7μg/mL,17.9μM,49.4μM and 29.1μM,respectively.展开更多
Background:To determine the efficacy of the Caulis Sargentodoxae prescription,which is an empirical formula of Chinese herbs and has definite curative effects on endometriosis.Methods:The Caulis Sargentodoxae prescrip...Background:To determine the efficacy of the Caulis Sargentodoxae prescription,which is an empirical formula of Chinese herbs and has definite curative effects on endometriosis.Methods:The Caulis Sargentodoxae prescription on the growth of ectopic endometria was studied with a rat endometriosis(EMS)model.The EMS model was established by autoplastic transplantation.To study the curative effects of Chinese medicine on EMS in comparison with western medicine,gestrinone and an angiogenesis inhibitor were introduced.The rats were randomly divided into seven groups:normal group,model group,ovariectomized group,gestrinone(western medicine)group,Caulis Sargentodoxae prescription(Chinese medicine)group,apatinib(inhibitor)group,and combination(Chinese medicine+inhibitor)group.After administration for 21 days,the growth inhibitory rates of ectopic endometria in the treatment groups were evaluated,and the levels of vascular endothelial growth factor(VEGF)and VEGF receptor-2(VEGFR2)were detected by ELISA in the serum and peritoneal fluid,as well as in the ectopic endometrial tissues by real-time polymerase chain reaction and Western blotting.Results:The growth inhibitory rates of ectopic endometria in the treatment groups were significantly higher(P<0.05).In the Caulis Sargentodoxae prescription group,the levels of angiogenesis-related factors,including VEGF and VEGFR2,were reduced in the serum and peritoneal fluid compared with the model group(P<0.05).In addition,the positive expression of VEGF and VEGFR2 in ectopic endometria significantly decreased in the Caulis Sargentodoxae prescription group both at mRNA and protein levels.Conclusions:VEGF and VEGFR2 levels in the serum and peritoneal fluid can be used as a clinical reference for endometriotic pathogenesis and treatment,and the Caulis Sargentodoxae prescription has reliable therapeutic effects on EMS for its target-action ability to decrease angiogenesis.展开更多
The dried cane of Kadsura interior(Kadsurae Caulis)is recorded in Chinese Pharmacopoeia 2010 as Dian Jixueteng for the treatment of rheumatism,irregular menstruation,and deficiency of Qi and blood.In this paper,morpho...The dried cane of Kadsura interior(Kadsurae Caulis)is recorded in Chinese Pharmacopoeia 2010 as Dian Jixueteng for the treatment of rheumatism,irregular menstruation,and deficiency of Qi and blood.In this paper,morphological characteristics,chemical constituents,and pharmacological activities in the cane of K.interior were summarized.Moreover,some suggestions about application and quality control of Kadsurae Caulis were proposed in order to provide a theoretical basis for further scientific use.展开更多
基金supported by the Science and Technology Program of Tianjin in China(No.23ZYJDSS00030)the Public Applied Technology Research Project of Zhejiang Province(No.LGD22H090010)。
文摘Background:Sargentodoxae Caulis(SC)is the vine stem of Sargentodoxa Cuneata(Oliv.)Rehd.&E.H.Wilson in C.S.Sargent,and it in traditional Chinese medicine has been known for promoting blood circulation and removing blood stasis as recorded in the ancient book“Illustrated Classics of Materia Medica”.It has been used effectively to treat blood stasis too in modern clinical practice.However,the anti-hyperlipidemia effect of SC is not fully understood.This paper aims at exploring the use of SC stems to improve the balance of blood lipids in the body,and its new role in treating hyperlipidemia.Methods:The effects of SC extract on hyperlipidemia were explored by combining lipidomics and gut microbiota.Secondly,we explored the potential mechanism of SC in treating hyperlipidemia by pathway analysis.Results:The results showed that the stem extract of SC could restore the physiological and biochemical indices of hyperlipidemia in mice,as well as repair the morphological and structural damage to tissues.Compared to the Model group,the SC extract significantly reduced the liver index,epididymal fat index,and Lee’s index.It also significantly decreased serum levels of total cholesterol,triglycerides,low-density lipoprotein cholesterol,peroxisome proliferator-activated receptor gamma(PPAR-γ),D-lactate,and free fatty acids,while significantly increasing the relative content of peroxisome proliferator-activated receptor alpha(PPAR-α).These changes were statistically significant.Non-targeted lipidomics,based on LC-MS,were utilized to investigate the lipid metabolism characteristics in serum,liver,and epididymal fat of the subjects.It was observed that,compared to the blank group,the Model group exhibited significant changes primarily in glycerol lipids and glycerophospholipids.The treatment group also displayed alterations in these lipids.A total of 38,81,and 27 differential lipids were identified in serum,liver,and epididymal fat samples,respectively.Among these,14 common differential lipids were found in both serum and liver samples,and their KEGG enrichment pathways were largely consistent.Among them,the sphingolipid signaling pathway and the glycerophospholipid metabolic pathway were identified as key metabolic pathways that were regulated.Our gut microbiota analysis revealed that SC diminishes the abundance of Actinobacteria by altering the cecal flora in mice.Conclusion:This alteration leads to the downregulation of genes involved in triglyceride metabolism,which in turn changes lipid processing and reduces triglyceride levels.Consequently,SC effectively combats hyperlipidemia.Notably,SC impacts key metabolic pathways,including the sphingolipid signaling and glycerophospholipid metabolism.These findings underscore SC’s therapeutic potential,positioning it as a promising alternative for reducing the health risks associated with hyperlipidemia.
基金supported by 2022 Anhui University Research Project(grant numbers No.2022AH051162)2023 Anhui University Collaborative innovation project(grant numbers No.GXXT-2023-074)National Key Clinical Collaboration Project for Difficult and Complex Diseases(Ovarian Malignancies)Integrating Traditional Chinese and Western Medicine(grant numbers National Traditional Chinese Medicine Comprehensive Development Document(2024)No.3).
文摘Background:Triple-negative breast cancer(TNBC)has long been a difficult therapeutic target to overcome because of its extreme aggressiveness,poor efficacy and rarity of available treatment regimens.To explore the anti-TNBC potential of Caulis Polygoni Multiflori(CPM),this investigation applied an integrated computational and experimental approach.Methods:Bioactive CPM compounds were investigated via screening of three TCM databases(TCMSP,TCMID,and HERB)using Bioavailability(OB≥30%)as inclusion criteria.TNBC targets were obtained from the online resources GeneCards,CTD,and OMIM network pharmacology analysis was used to determine common target(s),utilizing two PPI analysis tools–STRING and Cytoscape.Molecular docking assessed target-compound interactions by means of AutoDock Vina.In vitro studies conducted on MDA-MB-231 cells involved evaluating inhibitory effects via MTT,wound-healing assay,transwell,and qPCR techniques.Results:Six bioactive compounds were identified,with emodin(IC_(50) approximately 15μM)and chrysophanol(IC50 approximately 25μM)showing strong binding to TNBC targets(emodin-HSP90AB1:−9.42 kcal/mol;chrysophanol-SRC:−8.32 kcal/mol).Network analysis revealed 143 shared targets,with BCL2,EGFR and ESR1 as key nodes.Pathway analysis implicated PI3K-AKT signaling and apoptosis.In vitro,emodin and chrysophanol synergistically inhibited proliferation,migration and invasion,while downregulating BCL2,EGFR and ESR1 expression.Conclusion:To the best of our knowledge,this is the first report that describes the synergistic mechanisms of multiple compounds from the extract of CPM in TNBC with chrysophanol and emodin working as the main components by mediating oncogenic pathways.
文摘Objective:By using the method of network pharmacology to explore the mechanism of action of Caulis Sinomenii-Caulis Piperis Kadsurae herb pair in the treatment of rheumatoid arthritis.Methods:Search and screen the active ingredients and targets of Qingfengteng(Caulis Sinomenii)and Haifengteng(Caulis Piperis Kadsurae)through the TCMSP database,and predict and supplement the target points of the active ingredients through the Swiss Target Prediction platform;collect rheumatoid arthritis disease targets through GeneCards,OMIM database,and DRUGBANK database,construct a Venn diagram,and obtain the key target of Caulis Sinomenii-Caulis Piperis Kadsurae herb pairs in the treatment of rheumatoid arthritis;use Cytoscape 3.7.1 to establish the effect of Caysaceae sylvestris and Rhizoma sibirica in the treatment of rheumatoid arthritis"Drug-active ingredient-target"network diagram,submit key targets to STRING11.0 database to construct a protein interaction network model to obtain core targets;perform the GO and KEGG enrichment analysis of key targets on the Metascape platform,and draw the network diagram of"active pharmaceutical ingredients-targets-pathways".Results:After screening,discover a total of 27 active ingredients of Caulis Sinomenii-Caulis Piperis Kadsurae herb pairs,which can act on 229 targets,1269 related targets for rheumatoid arthritis diseases.And obtain a total of 95 common targets for drug active ingredients and diseases,including PTGS2,EGFR,MMP9,CASP3,JUN,etc.The common targets are mainly enriched in signaling pathways such as Pathways in cancer,Th17 cell differentiation and Endocrine resistance etc.Conclusion:This study preliminarily explored the potential mechanism of Caulis Sinomenii-Caulis Piperis Kadsurae herb pairs in the treatment of rheumatoid arthritis.The Caulis Sinomenii-Caulis Piperis Kadsurae herb pair may regulate cancer-related pathways,Th17 cell differentiation,endocrine resistance and other pathways to treat rheumatoid arthritis by regulating targets such as PTGS2,EGFR,and MMP9,providing clues and ideas for follow-up experimental research.
基金Supported by the National Natural Science Foundation of China(No.30571656).
文摘Matrix metalloproteinases（MMPs） play an essential role in development and tissue remodeling of living organisms. However, the overexpression of MMPs has lead to a series of diseases, such as cancer, arthritis, and atherosclerosis; and inhibition of MMPs may have therapeutic benefits. The discovery of MMP inhibitors from herbal has become a prospective event. We showed that the extract of Polygoni multiflori caulis from ethyl acetate or water（ethyl acetate extract and water extract） can inhibit the activities of MMPs 9, 14, and 16 in a dose-dependent manner and n-butyl alcohol extract of it can also inhibit these MMPs. Furthermore, we found that n-butyl alcohol extract and water extract of it influence the cell viability. These discoveries may contribute to the development of MMP in- hibitors for the therapy of a variety of pathological conditions.
基金supported by the International Scientific and Technological Cooperation Program of China(No:2009DFA31230)the Industry-University-Research Cooperation Program from Science and Technology Department of Qingyuang City(No:2012D021211001)
文摘Heteroclitin D （H.D） was successfully isolated from Kadsurae Caulis by using flash chromatography and recrystalllzed by methanol, 10.2 mg of H.D was obtained from 4.86 g of crude extract, and the purity determined by HPLC was 99.4%. The structure was identified by UV, IR, MS, and NMR analysis. The fast, simple and efficient method can be applied to the preparation of reference substance of H. D.
文摘There have appeared more and more antibiotics to antagonize against causative organism, but in comparison few antivirals have. Hence, many viral diseases remain refractory and fatal due to lack of effective medicine.Caulis Tripterygii wilfordii (TW) is a specific and potent Chinese medicine, and its effect in treating various intractable diseases, such as systemic lupus erythematosus, dermatomyositis, scleroderma, psoriasis and eczema has been confirmed by many medical researches. Pharmacological researches also proved that TW has anti-inflammatory and immunomodulatory actions. It is interesting that some effective components isolated from TW have an antiviral effect and more and more intention has been paid to related achievements at home and abroad.
文摘Objective To study the drug effects of ＂Caulis Sargentodoxae Formula＂ which dears away heat and blood clot on experimental endometriosis in rat, and to compare this effect with Danazol＇s effects. Method The model of endometriosis rat was induced by transplanting with endometrium surgically The rats were divided into 6 groups randomly： the non-treatment group, the castrate group, Danazol group（80 mg/kg）, ＂Caulis Sargentodoxae Formula＂ group （110 g/kg）, the medium dose group（77.5 g/kg） and the low dose group（55 g/kg）. After 3 weeks＇ treatment, the volume ofendometrium was detected by cutting the rats＇ belly open, drawing the materials from endometrium for tissue section, and doing the quantity analysis of endometrium by semiautomatic image analysis machine.Results There was no significant difference of the endometrium volumes between the low dose group and the non-treatment group（P〉O.05）, other groups＇ endometrium volumes were significantly lower than those of the non-treatment group（P〈0. 05）. And apart from the low dose group, all other groups＇ heights of the endometrium epithelia were significantly lower than that of the non-treatment group（p〈0.01）. Conclusion ＂Caulis Sargentodoxae Formula＂ can refrain the growth of the endometrium, and the effects are better than that of Danazol＇s.
基金Supported by National Natural Science Foundation of China(81460587)Zhuang Yao Medicine Collaborative InnovationC enter Project(GJKY[2013]20)+2 种基金Guangxi Key Laboratory of Zhuang Yao Medicine(GKJZ[2014]32)Guangxi Key Discipline:Zhuang Pharmacy(GJKY[2013]16)Chinese Traditional Medicine Innovation Theory and Drug EfficacyS tudy of Bagui Scholars
文摘This study was conducted to investigate the changes of polysaccharides content in Dendrobii Officinalis Caulis and its pharmacological action. The results showed that different growth years, different periods, different habitats and different external factors had influences on the change of polysaccharide content in Den- drobii Officinalis Caulis. Dendrobii Officinalis Caulis has certain effects of enhancing immunity, resisting fatigue and oxidation, promoting digestion and salivation secretion, reducing blood sugar and blood pressure, resisting tumor and liver injury, and relaxing bowels. Dendrobii Officinalis Caulis polysaccharides are the main effective components in Dendrobii Officinalis Caulis. The control of Dendrobii Officinalis Caulis polysaccharide content is of great significance to the assurance of the yield and quality of Dendrobii Officinalis Caulis material.
基金Supported by National Key Research and Development Program of China(2018YFC1708005)Sichuan Key Research and Development Program(2021YFS0043)+1 种基金Leading Talent Support Program of National Civil Commission(2021)Fundamental Research Funds for the Central Universities(ZYN2022067)。
文摘[Objectives]To establish the quality standard for Euonymi Grandiflorus Caulis and Folium.[Methods]Qualitative identification was performed by methods of origin identification,microscopic identification and thin layer chromatography(TLC);the moisture,total ash,acid-insoluble ash,and extract were detected using the method in the Chinese Pharmacopoeia(2020 Edition),and the contents of quercetin and kaempferol were determined by High Performance Liquid Chromatography(HPLC).[Results]The leaves of Euonymi Grandiflorus were subleathery,narrow and long elliptic or narrow obovate with cyme,and capsules were often with narrow wing ridge.Characteristics of microscopic identification were significant.Calcium oxalate cluster crystals,cubic crystals,inlaid parenchyma cells,starch granules,stomata,and fiber bundles could be observed.A TLC method for identification of Euonymi Grandiflorus Caulis and Folium was established.The contents of water,total ash,acid-insoluble ash were not higher than 13%,9%,12%,respectively;the extract were no less were not higher than 13%,9%,12%and 17%in ten batches of samples,respectively;the sum of quercetin and kaempferol were no less than 0.20%.[Conclusions]The quality standard of Euonymi Grandiflorus Caulis and Folium were established through the research.This method is accurate,specific,and reproducible and can be used for the quality control of Euonymi Grandiflorus Caulis and Folium.
基金Supported by Guangxi Key Research and Development Plan Project(Gui Ke AB18221095)2023 Innovation Project of Youjiang Medical University for Nationalities Graduate Education(YXCXJH2023009)Scientific Research Project of High-level Talents of Youjiang Medical University for Nationalities(01002018079).
文摘[Objectives]To explore the anti-tumor activity of the extracts of petroleum ether,ethyl acetate,n-butanol and aqueous solution from Mahoniae caulis.[Methods]The extracts were extracted with petroleum ether,ethyl acetate,n-butanol and aqueous solution respectively,and then concentrated.The inhibitory effects of these extracts on the growth of three tumor cell lines in vitro were detected by CCK-8 method,and the IC 50 value was calculated.[Results]The four extracts inhibited the growth of the three tumor cell lines in vitro,among which the n-butanol extract had the best anti-tumor activity.The IC 50 values of the n-butanol extract on human gastric cancer(SGC-7901),human breast cancer(MCF-7)and human liver cancer(BEL-7404)cell lines were 0.23,0.25 and 0.58 mg/mL,respectively.[Conclusions]The ethanol extract of Mahoniae caulis under petroleum ether,ethyl acetate,n-butanol and aqueous solution had certain anti-tumor effect,and n-butanol extract had the best anti-tumor activity.
文摘Mutong is a kind of medicine for promoting water circulation and drenching.There are many kinds of Aristolochia manshuriensis(Guan Mu Tong),which are commonly used in clinic,such as Mutong,Caulis Clematidis Armandii(Chuan Mu Tong)and Mutong.After the event of"Longdan Xiegan pill",the doctor made Chuanmu general taboo,or replaced it with other drugs,or did not dare to use it for a long time,which affected the clinical effect.This paper discusses the toxicity of Mutong.
文摘Objective:To study the effect of retention enema by caulis sargentodoxae enema combined with oral drugs on the immune inflammatory response and intestinal flora of UC patients. Methods: A total of 128 UC patients who received treatment in the hospital between January 2013 and April 2017 were collected and divided into control group and observation group by random number table. Control group received conventional oral drug therapy, and the observation group received retention enema by caulis sargentodoxae enema combined with oral drug therapy. Before treatment and after 4 weeks of treatment, the differences in the levels of immunoglobulin and inflammatory response indexes in serum as well as intestinal flora levels in mucosa lesion tissue were compared between the two groups.Results: The differences in levels of immunoglobulin and inflammatory response indexes in serum as well as intestinal flora levels in mucosa lesion tissue were not statistically significant between the two groups of patients before treatment. After 4 weeks of treatment, IgA, IgG, IgM and IL-4 contents in serum as well as bifidobacterium and lactobacillus levels in mucosa lesion tissue of observation group were higher than those of control group while ESR, CRP, IL-6 and TNF-αcontents as well as E. coli level in mucosa lesion tissue were lower than those of control group. Conclusion: Retention enema by caulis sargentodoxae enema combined with oral drugs can effectively optimize the immune inflammatory state and balance the intestinal flora distribution in patients with UC.
基金funded by National Natural Science Foundation of China(82060783)the Fund of State Key Laboratory of Phytochemistry and Plant Resources in West China,Chinese Academy of Sciences(P2022-KF13).
文摘Background:Trachelospermi Caulis et Folium,the stems and leaves of Trachelospermum jasminoides(Lindl.)Lem,has been used as a remedy for rheumatism,gonarthritis,backache and pharyngitis,according to the Chinese Pharmacopoeia.However,the corresponding chemical substances of this ethnic medicinal plant with anti-inflammatory activity kept unclear.This study aimed to explore the active substances attribute to its anti-rheumatic activity of this medical plant.Methods:Ultra-high performance liquid chromatography combined with quadrupole time-of-flight mass spectrometry was used to identify the chemical components in the extract of T.jasminoides.Molecular docking was applied to analyze the binding affinity of potential active lignans to inducible nitric oxide synthase enzyme.The anti-inflammatory effects of the potential active fraction were verified by cell experiments in vitro.Results:Forty-one compounds,mainly are lignans and nine of which were reported for the first time from the genus of Trachelospermum,were characterized using ultra-high performance liquid chromatography quadrupole-time-of-flight mass spectrometry method.Molecular docking results showed these identified lignans had excellent interactions with the inducible nitric oxide synthase enzyme,and the lignans-enriched fraction could significantly inhibit the release of nitric oxide and the expression of inducible nitric oxide synthase protein in LPS-induced RAW264.7 cells.Conclusion:Lignans are the main active substances that responsible for the anti-inflammatory activity of the ethnic herbal medicine T.jasminoides.
基金Financial support from the“Significant New Drugs Created”National Science and Technology Major Project of China(Nos.2012ZX09301003-001-004 and 2013ZX09102-018)。
文摘A new dibenzylbutyrolactone lignan named matairesinol 4'-O-β-D-cellobioside(1),together with a known compound(7R,85)-dihydrodehydrodiconiferyl alcohol(2)was isolated from Caulis Trachelospermi.The extract of Caulis Trachelospermi,which was separated by 80% alcohol extraction and subsequent HP-20 macroporous resin column chromatography,and its main components trachelogenin(3),nortrachelogenin(4),matairesinol(5)showed moderate inhibiting activities on NF-κB signaling pathway induced by TNFα,with the IC_(50) values of 17.7μg/mL,17.9μM,49.4μM and 29.1μM,respectively.
基金This work was supported by The Natural Science Foundation of China(grant no.81373684).
文摘Background:To determine the efficacy of the Caulis Sargentodoxae prescription,which is an empirical formula of Chinese herbs and has definite curative effects on endometriosis.Methods:The Caulis Sargentodoxae prescription on the growth of ectopic endometria was studied with a rat endometriosis(EMS)model.The EMS model was established by autoplastic transplantation.To study the curative effects of Chinese medicine on EMS in comparison with western medicine,gestrinone and an angiogenesis inhibitor were introduced.The rats were randomly divided into seven groups:normal group,model group,ovariectomized group,gestrinone(western medicine)group,Caulis Sargentodoxae prescription(Chinese medicine)group,apatinib(inhibitor)group,and combination(Chinese medicine+inhibitor)group.After administration for 21 days,the growth inhibitory rates of ectopic endometria in the treatment groups were evaluated,and the levels of vascular endothelial growth factor(VEGF)and VEGF receptor-2(VEGFR2)were detected by ELISA in the serum and peritoneal fluid,as well as in the ectopic endometrial tissues by real-time polymerase chain reaction and Western blotting.Results:The growth inhibitory rates of ectopic endometria in the treatment groups were significantly higher(P<0.05).In the Caulis Sargentodoxae prescription group,the levels of angiogenesis-related factors,including VEGF and VEGFR2,were reduced in the serum and peritoneal fluid compared with the model group(P<0.05).In addition,the positive expression of VEGF and VEGFR2 in ectopic endometria significantly decreased in the Caulis Sargentodoxae prescription group both at mRNA and protein levels.Conclusions:VEGF and VEGFR2 levels in the serum and peritoneal fluid can be used as a clinical reference for endometriotic pathogenesis and treatment,and the Caulis Sargentodoxae prescription has reliable therapeutic effects on EMS for its target-action ability to decrease angiogenesis.
文摘The dried cane of Kadsura interior(Kadsurae Caulis)is recorded in Chinese Pharmacopoeia 2010 as Dian Jixueteng for the treatment of rheumatism,irregular menstruation,and deficiency of Qi and blood.In this paper,morphological characteristics,chemical constituents,and pharmacological activities in the cane of K.interior were summarized.Moreover,some suggestions about application and quality control of Kadsurae Caulis were proposed in order to provide a theoretical basis for further scientific use.
