OBJECTIVE To investigate the relationship between the level of caspase-3 and 8 expression and intracellular Ca^2＋ levels in BCML-TA299 breast cancer cells in the process of apoptosis. METHODS Mice were divided into t...OBJECTIVE To investigate the relationship between the level of caspase-3 and 8 expression and intracellular Ca^2＋ levels in BCML-TA299 breast cancer cells in the process of apoptosis. METHODS Mice were divided into three IFNa-treated groups and one control group as follows： an intratumoral injection, subcutaneous injection, preventive injection, and a control without injection. The cellular DNA content, changes in the cell cycle and the relationship between the cell Ca^2＋ concentrations and the expression of cespase-3 and 8 were examined. RESULTS After injection of IFN-α-2b by different routes, the morphologic transformation of the breast cancer cells in each group was observed. There was a typical apoptotic response in the intratumoral-Jnjection group. The expression of cespase-3 and 8 was diverse among the experimental groups, and correlated with the cellular Ca^2＋ concentration. Caspase-3 and 8 expression and the cellular Ca^2＋ level were higher following intratumoral injection compared to the other treatments （P〈0.01）. Among the experimental groups, the cell cycle displayed definitive changes. CONCLUSION a） Both caspase-3 and 8 and the intracellular Ca^2＋ are elevated in the process of cell apoptosis in BCML-TA299 breast cancer tissues. These changes may play important roles in the occurrence and development of breast cancer; b） Variation in the route of IFN-α-2b administration can produce different responses in the expression of caspase-3 and 8 and the concentration of Ca^2＋ in apoptotic tumor cells.展开更多
文摘OBJECTIVE To investigate the relationship between the level of caspase-3 and 8 expression and intracellular Ca^2＋ levels in BCML-TA299 breast cancer cells in the process of apoptosis. METHODS Mice were divided into three IFNa-treated groups and one control group as follows： an intratumoral injection, subcutaneous injection, preventive injection, and a control without injection. The cellular DNA content, changes in the cell cycle and the relationship between the cell Ca^2＋ concentrations and the expression of cespase-3 and 8 were examined. RESULTS After injection of IFN-α-2b by different routes, the morphologic transformation of the breast cancer cells in each group was observed. There was a typical apoptotic response in the intratumoral-Jnjection group. The expression of cespase-3 and 8 was diverse among the experimental groups, and correlated with the cellular Ca^2＋ concentration. Caspase-3 and 8 expression and the cellular Ca^2＋ level were higher following intratumoral injection compared to the other treatments （P〈0.01）. Among the experimental groups, the cell cycle displayed definitive changes. CONCLUSION a） Both caspase-3 and 8 and the intracellular Ca^2＋ are elevated in the process of cell apoptosis in BCML-TA299 breast cancer tissues. These changes may play important roles in the occurrence and development of breast cancer; b） Variation in the route of IFN-α-2b administration can produce different responses in the expression of caspase-3 and 8 and the concentration of Ca^2＋ in apoptotic tumor cells.
