The objective of this study was to determine the optimal proportions of plant growth regulators for growth and non-tube rapid propagation of Cymbidium tortisepalum var. longibracteatum;seedlings were utilized as the m...The objective of this study was to determine the optimal proportions of plant growth regulators for growth and non-tube rapid propagation of Cymbidium tortisepalum var. longibracteatum;seedlings were utilized as the material. The effects of various combinations and concentrations of 6-benzylaminopurine (6-BA), gibberellic acid (GA_(3)), and naphthaleneacetic acid (NAA) on growth and non-tube rapid propagation were assessed through a single-factor testing and response surface methodology. The results indicated that 6-BA at 60 mg/L, GA_(3) at 150 mg/L, and NAA at 30 mg/L were the most effective concentrations for promoting leaf buds formation in the single-factor analysis. Response surface methodology clarified the sensitivity of the proliferation rate of lateral buds to the three factors, with 6-BA being the most influential, followed by GA_(3) and NAA. The increase in leaf area was most significantly influenced by NAA, then GA_(3), and least by 6-BA, while the increase in plant height was most responsive to GA_(3), followed by 6-BA, and then NAA. The ideal concentrations of plant growth regulators were established as 6-BA at 43 mg/L, GA_(3) at 169 mg/L, and NAA at 36 mg/L. Under these conditions, the lateral bud number per plant was 2.78, with a leaf area increment of 2.87 cm2 and a plant height increment of 2.67 cm.展开更多
The infrageneric classification currently in use for Cymbidium is based on gross morphology, with emphasis on the number of pollinia and state of fusion between lip and column. The sequences of nrDNA regions of 27...The infrageneric classification currently in use for Cymbidium is based on gross morphology, with emphasis on the number of pollinia and state of fusion between lip and column. The sequences of nrDNA regions of 27 species and 3 cultivars of Cymbidium and 3 outgroup species ( Eulophia graminea, Geodorum densiflorum, Amitostigma pinguiculum) were analyzed using PCR amplification and direct DNA sequencing. The phylogenetic trees generated from maximum parsimony analysis, however, show that the existing division among three subgenera (subgen. Cymbidium , subgen. Cyperorchis and subgen. Jensoa ) should be evaluated with more data. Subgenus Cyperorchis was not a monophyletic group, with the unexpected nesting of C. dayanum (subgen. Cymbidium ) within it; subgenus Jensoa also appeared paraphyletic, with C. lancifolium being the sister group to the remainder of the genus; species of subgen. Cymbidium appeared polyphyletic, being split into several clades and intermixed with the main subgen. Cyperorchis and subgen. Jensoa clades, respectively. However, because of the insufficiency of informative characters of ITS sequences, some of the clades identified, especially the major lineages of Cymbidium , received relatively low support; sectional delimitations were also not clear within each subgenus. Further study is needed for achieving a robust phylogeny of Cymbidium .展开更多
Effects of two media and fertilizer levels on the growth of Cymbidium hybridium were studied. Results demonstrated that peanut\|hull mixed sand (abbreviated as PH/S=1/1) medium had higher total porosity and larger...Effects of two media and fertilizer levels on the growth of Cymbidium hybridium were studied. Results demonstrated that peanut\|hull mixed sand (abbreviated as PH/S=1/1) medium had higher total porosity and larger air space and lower water holding capacity in comparison with sphagnum (abbreviated as SP) medium. Leaf growth rate, chlorophyll contents, fresh weight and photosynthetic rate change along with the changes of nitrogen concentrations of both SP and PH/S media. According to the investigation,when the nitrogen concentration was 222 75?mg·L -1 , the photosynthetic rate of SP\|medium reached the highest. The practical nitrogen content was 180 00?mg·L -1 for PH/S medium . The maximum of flower quantity (number per spike) was gotten by treating of N/P 2O 5/K 2O=10/30/20 on PH/S medium, and N/P 2O 5/K 2O=0/23/29 treatment was the lowest on PH/S medium. The differences in small flowers, stem length and flower quantity among the two media and the three fertilization methods reached significant at 0 05 level,respectively.展开更多
[Objective] The aim of the research was to establish asymbiotic germination and low-temperature in vitro conservation technique system of Cymbidium dayanum by using plant tissue culture technique to realize its rapid ...[Objective] The aim of the research was to establish asymbiotic germination and low-temperature in vitro conservation technique system of Cymbidium dayanum by using plant tissue culture technique to realize its rapid propagation and long-term conservation in vitro. [Method] With mature seeds of C. dayanum as explants, different media were selected to establish asymbiotic germination technique system. With protocorms as materials, conservation, resumptive proliferation and plant regeneration conditions were selected to establish low-temperature in vitro conservation technique system preliminarily. [Result] Mature seeds of C. dayanum could germinate after cultured 90 days on MS media as well as ＂Hyponex 1＂ media. The germination rate reached more than 98%. Protocorms inoculated in ＂Hyponex 1＂ media could be conserved continuously at 5 ℃ in dark for more than 18 months and the survival rate could reach 90%. Conserved protocorms could realize resumptive preliferation culture both on 1/2 MS and ＂Hyponex 1＂ media. The seed- ling-strengthening and rooting media were 1/2 MS media. [Conclusion] This research provided practical basis for in vitro conservation and rapid propagation of C. dayanum germplasm resource.展开更多
An intermediate between Cymbidium lancifolium Hook. and C.ensifolium (L.) Sw. is described as a new hybrid of them: C.× oblancifolium I. J. Liu et S. C. Chen. It is widespread in southern Sic huan Province in so...An intermediate between Cymbidium lancifolium Hook. and C.ensifolium (L.) Sw. is described as a new hybrid of them: C.× oblancifolium I. J. Liu et S. C. Chen. It is widespread in southern Sic huan Province in southwestern China . After being introduced into cultivation, it remains steady in its character an d indicates remarkable growth. It also exhibits great aesthetic values.展开更多
[Objective] This study was conducted to screen the best fertilization mode for potted Cymbidium sinense ＇Qihei＇. [Method] According to the contents of the N, P and K elements in C. sinense ＇Qihei＇ and the main fer...[Objective] This study was conducted to screen the best fertilization mode for potted Cymbidium sinense ＇Qihei＇. [Method] According to the contents of the N, P and K elements in C. sinense ＇Qihei＇ and the main fertilization modes used by producers and related literature information, the effects of 8 kinds of fertilization models on the growth of C. sinense ＇Qihei＇ were studied. Twenty six morphologic and physiological indexes of C. sinense ＇Qihei＇ growth were measured. [Result] Significant differences were shown on 21 indexes. [Conclusion] Based on the 26 in- dexes, the best fertilization mode included the steps of applying 14-14-14 （N-P20^- K20） slow-release fertilizer particles （8 g per bag） once in April, July and October, respectively; drip-applying 800 times of 30-10-10 water-soluble fertilizer once every half a month from April to September; and drip-applying 800 times of 15-5-30 wa- ter-soluble fertilizer （150 ml） once every half a month from October to December.展开更多
[Objective] By using the genomic DNA of Cymbidium faberi Rolfe as template,the factors that affect the result of ISSR-PCR reaction system were researched and the optimal system was established.[Method] The genomic DNA...[Objective] By using the genomic DNA of Cymbidium faberi Rolfe as template,the factors that affect the result of ISSR-PCR reaction system were researched and the optimal system was established.[Method] The genomic DNA was extracted from C.faberi Rolfe with method of modified CTAB.Different factors which affected ISSR amplification reaction were optimized.[Result] High-quality genomic DNA was obtained from C.faberi Rolfe.And the optimal reaction system was as follows：25 μl amplification reactions system contained 2.5 μl 10 × PCR buffer,2.0 mmol/L MgCl2,60 ng template DNA,160 μmol/L dNTPs,1.25 U Taq DNA polymerase,0.4 μmol/L ISSR primer and 15.85 μl ddH2O.The optimal amplification procedures were pre-denaturing for 5 min at 94 ℃,followed by 40 cycles of denaturing for 30 s at 94 ℃,annealing for 30 s at a temperature of 2-3 ℃ lower than melting temperature of each primer pair,extension for 50 s at 72 ℃.Then extension step of 7 min at 72 ℃ was performed.[Conclusion] The optimal system could provide a favorable basis for further study on genetic diversity of C.faberi Rolfe by using ISSR molecular marker technique.展开更多
基金funded by the Sichuan Science and Technology Program“Research on Synergistic Relationship and Reintroduction Technology of C.tortisepalum var.longibracteatum and Endophytic Mycorrhizal Fungi in Eastern Sichuan”(2017JY0132)Longshan Academic Talent Research Supporting Program of SWUST(17LZX521,18LZX522).
文摘The objective of this study was to determine the optimal proportions of plant growth regulators for growth and non-tube rapid propagation of Cymbidium tortisepalum var. longibracteatum;seedlings were utilized as the material. The effects of various combinations and concentrations of 6-benzylaminopurine (6-BA), gibberellic acid (GA_(3)), and naphthaleneacetic acid (NAA) on growth and non-tube rapid propagation were assessed through a single-factor testing and response surface methodology. The results indicated that 6-BA at 60 mg/L, GA_(3) at 150 mg/L, and NAA at 30 mg/L were the most effective concentrations for promoting leaf buds formation in the single-factor analysis. Response surface methodology clarified the sensitivity of the proliferation rate of lateral buds to the three factors, with 6-BA being the most influential, followed by GA_(3) and NAA. The increase in leaf area was most significantly influenced by NAA, then GA_(3), and least by 6-BA, while the increase in plant height was most responsive to GA_(3), followed by 6-BA, and then NAA. The ideal concentrations of plant growth regulators were established as 6-BA at 43 mg/L, GA_(3) at 169 mg/L, and NAA at 36 mg/L. Under these conditions, the lateral bud number per plant was 2.78, with a leaf area increment of 2.87 cm2 and a plant height increment of 2.67 cm.
文摘The infrageneric classification currently in use for Cymbidium is based on gross morphology, with emphasis on the number of pollinia and state of fusion between lip and column. The sequences of nrDNA regions of 27 species and 3 cultivars of Cymbidium and 3 outgroup species ( Eulophia graminea, Geodorum densiflorum, Amitostigma pinguiculum) were analyzed using PCR amplification and direct DNA sequencing. The phylogenetic trees generated from maximum parsimony analysis, however, show that the existing division among three subgenera (subgen. Cymbidium , subgen. Cyperorchis and subgen. Jensoa ) should be evaluated with more data. Subgenus Cyperorchis was not a monophyletic group, with the unexpected nesting of C. dayanum (subgen. Cymbidium ) within it; subgenus Jensoa also appeared paraphyletic, with C. lancifolium being the sister group to the remainder of the genus; species of subgen. Cymbidium appeared polyphyletic, being split into several clades and intermixed with the main subgen. Cyperorchis and subgen. Jensoa clades, respectively. However, because of the insufficiency of informative characters of ITS sequences, some of the clades identified, especially the major lineages of Cymbidium , received relatively low support; sectional delimitations were also not clear within each subgenus. Further study is needed for achieving a robust phylogeny of Cymbidium .
文摘Effects of two media and fertilizer levels on the growth of Cymbidium hybridium were studied. Results demonstrated that peanut\|hull mixed sand (abbreviated as PH/S=1/1) medium had higher total porosity and larger air space and lower water holding capacity in comparison with sphagnum (abbreviated as SP) medium. Leaf growth rate, chlorophyll contents, fresh weight and photosynthetic rate change along with the changes of nitrogen concentrations of both SP and PH/S media. According to the investigation,when the nitrogen concentration was 222 75?mg·L -1 , the photosynthetic rate of SP\|medium reached the highest. The practical nitrogen content was 180 00?mg·L -1 for PH/S medium . The maximum of flower quantity (number per spike) was gotten by treating of N/P 2O 5/K 2O=10/30/20 on PH/S medium, and N/P 2O 5/K 2O=0/23/29 treatment was the lowest on PH/S medium. The differences in small flowers, stem length and flower quantity among the two media and the three fertilization methods reached significant at 0 05 level,respectively.
基金Research supported by national science and technology basic conditions platform program(2005DKA21000-5-63).~~
文摘[Objective] The aim of the research was to establish asymbiotic germination and low-temperature in vitro conservation technique system of Cymbidium dayanum by using plant tissue culture technique to realize its rapid propagation and long-term conservation in vitro. [Method] With mature seeds of C. dayanum as explants, different media were selected to establish asymbiotic germination technique system. With protocorms as materials, conservation, resumptive proliferation and plant regeneration conditions were selected to establish low-temperature in vitro conservation technique system preliminarily. [Result] Mature seeds of C. dayanum could germinate after cultured 90 days on MS media as well as ＂Hyponex 1＂ media. The germination rate reached more than 98%. Protocorms inoculated in ＂Hyponex 1＂ media could be conserved continuously at 5 ℃ in dark for more than 18 months and the survival rate could reach 90%. Conserved protocorms could realize resumptive preliferation culture both on 1/2 MS and ＂Hyponex 1＂ media. The seed- ling-strengthening and rooting media were 1/2 MS media. [Conclusion] This research provided practical basis for in vitro conservation and rapid propagation of C. dayanum germplasm resource.
文摘An intermediate between Cymbidium lancifolium Hook. and C.ensifolium (L.) Sw. is described as a new hybrid of them: C.× oblancifolium I. J. Liu et S. C. Chen. It is widespread in southern Sic huan Province in southwestern China . After being introduced into cultivation, it remains steady in its character an d indicates remarkable growth. It also exhibits great aesthetic values.
基金Supported by Science and Technology Planning Project of Guangdong Province(2012A020602036)
文摘[Objective] This study was conducted to screen the best fertilization mode for potted Cymbidium sinense ＇Qihei＇. [Method] According to the contents of the N, P and K elements in C. sinense ＇Qihei＇ and the main fertilization modes used by producers and related literature information, the effects of 8 kinds of fertilization models on the growth of C. sinense ＇Qihei＇ were studied. Twenty six morphologic and physiological indexes of C. sinense ＇Qihei＇ growth were measured. [Result] Significant differences were shown on 21 indexes. [Conclusion] Based on the 26 in- dexes, the best fertilization mode included the steps of applying 14-14-14 （N-P20^- K20） slow-release fertilizer particles （8 g per bag） once in April, July and October, respectively; drip-applying 800 times of 30-10-10 water-soluble fertilizer once every half a month from April to September; and drip-applying 800 times of 15-5-30 wa- ter-soluble fertilizer （150 ml） once every half a month from October to December.
基金Supported by a Program from the State Environmental Protection Administration(20061A0013)~~
文摘[Objective] By using the genomic DNA of Cymbidium faberi Rolfe as template,the factors that affect the result of ISSR-PCR reaction system were researched and the optimal system was established.[Method] The genomic DNA was extracted from C.faberi Rolfe with method of modified CTAB.Different factors which affected ISSR amplification reaction were optimized.[Result] High-quality genomic DNA was obtained from C.faberi Rolfe.And the optimal reaction system was as follows：25 μl amplification reactions system contained 2.5 μl 10 × PCR buffer,2.0 mmol/L MgCl2,60 ng template DNA,160 μmol/L dNTPs,1.25 U Taq DNA polymerase,0.4 μmol/L ISSR primer and 15.85 μl ddH2O.The optimal amplification procedures were pre-denaturing for 5 min at 94 ℃,followed by 40 cycles of denaturing for 30 s at 94 ℃,annealing for 30 s at a temperature of 2-3 ℃ lower than melting temperature of each primer pair,extension for 50 s at 72 ℃.Then extension step of 7 min at 72 ℃ was performed.[Conclusion] The optimal system could provide a favorable basis for further study on genetic diversity of C.faberi Rolfe by using ISSR molecular marker technique.
