Objeclive:To evaluate the larvicidal activity of the tuber extracts isolated from two species of Cyclamen（Cyxltimen mimbile Hildebr.and Cyclamen alpinum Dammann ex.Sprcnger） against Culex pipiens L.（Diptera:Culici...Objeclive:To evaluate the larvicidal activity of the tuber extracts isolated from two species of Cyclamen（Cyxltimen mimbile Hildebr.and Cyclamen alpinum Dammann ex.Sprcnger） against Culex pipiens L.（Diptera:Culicidae）.Methods:Twenty young（first-second） or older（third- fourth） instars of tile Culex pipiens were exposed to various concentrations（100 to I 000 ppm） of the extracts.Mortality was recorded after 12-,24-,48-.72- and 96-h of exposure.Results:We determined that the young larval stages were more susceptible to the extracts in comparison with the older larval stages.A comparison of LC<sub>90</sub> and LC<sub>90</sub> values has shown that Cyclamen mimbile was more active than Cyclamen alpinum.Conclusions:This study is the first to report on the larvicidal activity of the extracts of Cyclamen species against Culex pipiens.More studies are needed to isolate and identify the active components involved,their mode of action,and effects on other mosquito and pest species.展开更多
Five kinds of agricultural castoffs, such as sawdust and powder of coconut coir, were used as growing media to substitute the peat moss for potted Cyclamen percicum. It showed that most of the substitute media could f...Five kinds of agricultural castoffs, such as sawdust and powder of coconut coir, were used as growing media to substitute the peat moss for potted Cyclamen percicum. It showed that most of the substitute media could fit for the growth of the root system without disturbing the spatial development of the root. The root activities of the plants in substitute media were all higher or not significantly lower than that in the contrast peat moss （PM）. The substitute media might not make adverse effects on the biosynthesis of chlorophyll of C. percicum leaves, and the nutrient components contained in the substitute media itself was none of the content of mineral elements in the leaves. The morphological indexes of C. percicum plants growing in the substitute media except in the powder of coconut coir （PCC） were all better than those in the contrast PM. The synthetically evaluation index of the plant showed that the sawdust, the powder of maize core, the powder of soybean stalk, the powder of peanut hull could take place the peat moss totally in C. percicum industrialization cultivation.展开更多
Cyclamen leaves and petioles explants were cultured on MS media supplemented with different concentrations of 2, 4-dichlorophenoxyacetic acid (2, 4-D) or 1-naphthaleneacetic acid (NAA) to induce callus. The effect of ...Cyclamen leaves and petioles explants were cultured on MS media supplemented with different concentrations of 2, 4-dichlorophenoxyacetic acid (2, 4-D) or 1-naphthaleneacetic acid (NAA) to induce callus. The effect of 2, 4-D on shoot regeneration was also studied. Either in media containing 2, 4-D or in media containing NAA, callus was observed, but the quality or quantity of callus induced by 2, 4-D or NAA were different. The callus induced by 2, 4-D was white, compact and having powerful multiplication capacity. The callus was inclined to browning then was poorly organogenetic. While the callus induced by NAA was yellowish in appearance. It was pultaceous and proliferated bradytelicly. The callus usually can give rise to many shoots. But the frequency of inducing callus of 2, 4-D is higher than that of NAA. The regenerative plantlets derived from the callus respectively induced by 2, 4-D or NAA were transferred into rooting medium. The frequency of rooting were no difference.展开更多
In order to explore the heredity of leaf veins of Cyclamen Hederifolium and to breed excellent varieties, selling measurement for six types of different leaf veins were carried out and the genetie constitutions of lea...In order to explore the heredity of leaf veins of Cyclamen Hederifolium and to breed excellent varieties, selling measurement for six types of different leaf veins were carried out and the genetie constitutions of leaf veins were studied according to the separation conditions of their progenies. The results showed that the inbred progenies of B or M types were B or M types with a percentage of 100% while the progenies of F, H, X and L types had character segregations. The separa- tion law illustrated that leaf veins of Cyclamen Hederifolium were eontrolled by minor multiple genes and each locus was consisted of a pair of alleles, RL of Rn. RL was responsible for the green phenotype of leaves and RB was responsible for the silvery white phenotype of leaves, leaves were deep green when the genotype of the locus was RLRL ; leaves were green when the genotype was RLRB ; leaves were silvery white when the genotype was RBRB. The aggregafive pattern of each locus formed different leaf vein types. The gene control modes of leaf vein variations were the basis for the breeding of excellent varieties of Cyclamen Hederifolium.展开更多
In this study, antioxidant, cytotoxic, larvicidal, antimicrobial and anthelmintic effects and phenolic contents of ethanol, methanol and acetone extracts of leaf and tuber parts of Cyclamen alpinum were investigated. ...In this study, antioxidant, cytotoxic, larvicidal, antimicrobial and anthelmintic effects and phenolic contents of ethanol, methanol and acetone extracts of leaf and tuber parts of Cyclamen alpinum were investigated. DPPH, ABTS, β-carotene assays were carried out in antioxidant activity and total phenolic and flavonoid contents were tested in determination assay. 9 phenolic contents were determined by HPLC. Artemia salina was used in the cytotoxic effect. Larvicidal effect was investigated against Culex pipiens. Disc diffusion method was used in antimicrobial effect. The tuber part was found to be more toxic than the leaf part in the anthelmintic activity assay. The highest value obtained from the antioxidant activity experiment was observed with value of 86.73 ± 0.16 (%) in DPPH assay. The lowest LC50 value in larvicidal effect was determined 0.151 mg/mL after 72 hours. Consequently, there is need for further studies on the Cyclamen alpinum, which has the ability to fight against diseases such as cancer, cardiovascular diseases, etc.展开更多
为建立适宜的仙客来耐热评价体系并筛选耐热性种质,本研究选取20个主栽仙客来品种,进行每日4 h 40℃高温胁迫处理,在胁迫0、5、10、15 d后系统测定形态与生理生化指标,通过相关性分析、隶属函数分析、聚类分析和逐步回归分析及多指标建...为建立适宜的仙客来耐热评价体系并筛选耐热性种质,本研究选取20个主栽仙客来品种,进行每日4 h 40℃高温胁迫处理,在胁迫0、5、10、15 d后系统测定形态与生理生化指标,通过相关性分析、隶属函数分析、聚类分析和逐步回归分析及多指标建模等方法综合评价品种耐热性。结果表明:高温胁迫显著改变植株表型及生理参数,品种间响应模式存在显著差异(P<0.05);隶属函数分析显示耐热性综合评价值(D值)变异范围为0.271~0.695,其中CP16(D=0.695)耐热性最优,CP6(D=0.271)最敏感;聚类分析将供试品种分为3类:耐热型(CP16)、中间型(CP2、CP3、CP7等13个品种)及敏感型(CP1、CP4、CP5、CP6、CP18、CP20);通过多元回归分析,建立了仙客来品种的耐热性评价模型,筛选出5个评价指标(叶宽、叶片含水率、Pro、SOD、Fv/Fo)。本研究建立的评价体系为仙客来耐热品种选育提供了理论依据与技术支撑。展开更多
To identify the theoretic foundation for maintaining and improving high quality varieties and breeding new F1 hybrids, flower color segregation occurred in the offspring in both self-pollination and cross- pollination...To identify the theoretic foundation for maintaining and improving high quality varieties and breeding new F1 hybrids, flower color segregation occurred in the offspring in both self-pollination and cross- pollination of varieties of different colors were investigated. The main results indicate that the heredity of Cyclamen persicum color follows both quantitative and qualitative hereditary regularity. All genes can be classified as two groups of the throat-gene and coronal-gene occupying different spots on the chromosome. The colored gene is the dominant gene, while the white gene is the recessive one. The gene groups and their quantity determine the color of cyclamen persicum. And sometimes the throat- gene and the coronal-gene take a recombination, forming a new type of flower.展开更多
基金the Scientific Projects Administration Unit of Akdeniz University.Turkey for providing financial support
文摘Objeclive:To evaluate the larvicidal activity of the tuber extracts isolated from two species of Cyclamen（Cyxltimen mimbile Hildebr.and Cyclamen alpinum Dammann ex.Sprcnger） against Culex pipiens L.（Diptera:Culicidae）.Methods:Twenty young（first-second） or older（third- fourth） instars of tile Culex pipiens were exposed to various concentrations（100 to I 000 ppm） of the extracts.Mortality was recorded after 12-,24-,48-.72- and 96-h of exposure.Results:We determined that the young larval stages were more susceptible to the extracts in comparison with the older larval stages.A comparison of LC<sub>90</sub> and LC<sub>90</sub> values has shown that Cyclamen mimbile was more active than Cyclamen alpinum.Conclusions:This study is the first to report on the larvicidal activity of the extracts of Cyclamen species against Culex pipiens.More studies are needed to isolate and identify the active components involved,their mode of action,and effects on other mosquito and pest species.
文摘Five kinds of agricultural castoffs, such as sawdust and powder of coconut coir, were used as growing media to substitute the peat moss for potted Cyclamen percicum. It showed that most of the substitute media could fit for the growth of the root system without disturbing the spatial development of the root. The root activities of the plants in substitute media were all higher or not significantly lower than that in the contrast peat moss （PM）. The substitute media might not make adverse effects on the biosynthesis of chlorophyll of C. percicum leaves, and the nutrient components contained in the substitute media itself was none of the content of mineral elements in the leaves. The morphological indexes of C. percicum plants growing in the substitute media except in the powder of coconut coir （PCC） were all better than those in the contrast PM. The synthetically evaluation index of the plant showed that the sawdust, the powder of maize core, the powder of soybean stalk, the powder of peanut hull could take place the peat moss totally in C. percicum industrialization cultivation.
文摘Cyclamen leaves and petioles explants were cultured on MS media supplemented with different concentrations of 2, 4-dichlorophenoxyacetic acid (2, 4-D) or 1-naphthaleneacetic acid (NAA) to induce callus. The effect of 2, 4-D on shoot regeneration was also studied. Either in media containing 2, 4-D or in media containing NAA, callus was observed, but the quality or quantity of callus induced by 2, 4-D or NAA were different. The callus induced by 2, 4-D was white, compact and having powerful multiplication capacity. The callus was inclined to browning then was poorly organogenetic. While the callus induced by NAA was yellowish in appearance. It was pultaceous and proliferated bradytelicly. The callus usually can give rise to many shoots. But the frequency of inducing callus of 2, 4-D is higher than that of NAA. The regenerative plantlets derived from the callus respectively induced by 2, 4-D or NAA were transferred into rooting medium. The frequency of rooting were no difference.
基金Supported by"Introduction of Germplasm Resources and the Distant Hybridization Techniques of Cyclamen Hederifolium"of"948"Introduced Project of State Forestry Bureau(2013-4-42)
文摘In order to explore the heredity of leaf veins of Cyclamen Hederifolium and to breed excellent varieties, selling measurement for six types of different leaf veins were carried out and the genetie constitutions of leaf veins were studied according to the separation conditions of their progenies. The results showed that the inbred progenies of B or M types were B or M types with a percentage of 100% while the progenies of F, H, X and L types had character segregations. The separa- tion law illustrated that leaf veins of Cyclamen Hederifolium were eontrolled by minor multiple genes and each locus was consisted of a pair of alleles, RL of Rn. RL was responsible for the green phenotype of leaves and RB was responsible for the silvery white phenotype of leaves, leaves were deep green when the genotype of the locus was RLRL ; leaves were green when the genotype was RLRB ; leaves were silvery white when the genotype was RBRB. The aggregafive pattern of each locus formed different leaf vein types. The gene control modes of leaf vein variations were the basis for the breeding of excellent varieties of Cyclamen Hederifolium.
文摘In this study, antioxidant, cytotoxic, larvicidal, antimicrobial and anthelmintic effects and phenolic contents of ethanol, methanol and acetone extracts of leaf and tuber parts of Cyclamen alpinum were investigated. DPPH, ABTS, β-carotene assays were carried out in antioxidant activity and total phenolic and flavonoid contents were tested in determination assay. 9 phenolic contents were determined by HPLC. Artemia salina was used in the cytotoxic effect. Larvicidal effect was investigated against Culex pipiens. Disc diffusion method was used in antimicrobial effect. The tuber part was found to be more toxic than the leaf part in the anthelmintic activity assay. The highest value obtained from the antioxidant activity experiment was observed with value of 86.73 ± 0.16 (%) in DPPH assay. The lowest LC50 value in larvicidal effect was determined 0.151 mg/mL after 72 hours. Consequently, there is need for further studies on the Cyclamen alpinum, which has the ability to fight against diseases such as cancer, cardiovascular diseases, etc.
文摘为建立适宜的仙客来耐热评价体系并筛选耐热性种质,本研究选取20个主栽仙客来品种,进行每日4 h 40℃高温胁迫处理,在胁迫0、5、10、15 d后系统测定形态与生理生化指标,通过相关性分析、隶属函数分析、聚类分析和逐步回归分析及多指标建模等方法综合评价品种耐热性。结果表明:高温胁迫显著改变植株表型及生理参数,品种间响应模式存在显著差异(P<0.05);隶属函数分析显示耐热性综合评价值(D值)变异范围为0.271~0.695,其中CP16(D=0.695)耐热性最优,CP6(D=0.271)最敏感;聚类分析将供试品种分为3类:耐热型(CP16)、中间型(CP2、CP3、CP7等13个品种)及敏感型(CP1、CP4、CP5、CP6、CP18、CP20);通过多元回归分析,建立了仙客来品种的耐热性评价模型,筛选出5个评价指标(叶宽、叶片含水率、Pro、SOD、Fv/Fo)。本研究建立的评价体系为仙客来耐热品种选育提供了理论依据与技术支撑。
基金The project was funded by Hebei Forestry Bureau (0008261)
文摘To identify the theoretic foundation for maintaining and improving high quality varieties and breeding new F1 hybrids, flower color segregation occurred in the offspring in both self-pollination and cross- pollination of varieties of different colors were investigated. The main results indicate that the heredity of Cyclamen persicum color follows both quantitative and qualitative hereditary regularity. All genes can be classified as two groups of the throat-gene and coronal-gene occupying different spots on the chromosome. The colored gene is the dominant gene, while the white gene is the recessive one. The gene groups and their quantity determine the color of cyclamen persicum. And sometimes the throat- gene and the coronal-gene take a recombination, forming a new type of flower.
