OBJECTIVE: To investigate the effects of electroacupuncture(EA) at the Guanyuan(CV 4) or Sanyinjiao(SP 6) acupoints on the hypothalamus-pituitary-ovary(HPO) axis and spatial learning and memory in female mice.METHODS:...OBJECTIVE: To investigate the effects of electroacupuncture(EA) at the Guanyuan(CV 4) or Sanyinjiao(SP 6) acupoints on the hypothalamus-pituitary-ovary(HPO) axis and spatial learning and memory in female mice.METHODS: Nine-month-old female mice with senescence-accelerated mouse prone 8(SAMP8)were divided into three groups: the disease model,EA-Guanyuan and EA-Sanyinjiao groups. Concurrently, 9-month old female mice with senescence-accelerated mouse resistance 1(SAMR1)were set as the control model group. The two treatment groups were given the same pattern of EA stimulation. Gonadotropin-releasing hormone, luteinizing hormone, follicle-stimulating hormone(FSH) and Serum estradiol levels in the Hypothalamus-pituitary-ovary axis were assessed by enzyme-linked immunosorbent assay to determinethe HPO axis function level. Spatial learning and memory were assessed by the Morris Water Maze(MWM) test.RESULTS:(a) HPO axis: compared with the control model group, the disease model group displayed a decrease in E2 levels(P < 0.01), and an increase in Gn RH, LH and FSH levels(P < 0.01). E2 levels were increased in EA treatment groups compared with the disease model group(P < 0.05). In contrast,Gn RH and LH and FSH levels were reduced(P <0.05). EA-Sanyinjiao group was superior than EA-Guanyuan group on increasing E2 and declining Gn RH levels(P < 0.01).(b) The MWM test demonstrated that the response latency in the EA-Sanyinjiao treatment group declined from day 2 to day5 compared with the disease model group(P <0.05), whereas the EA-Guanyuan treatment group showed no significant difference.CONCLUSION: EA can regulate hormone(E2, FSH,LH, Gn RH) levels in the HPO axis and the spatial learning and memory ability in female SAMP8 mice. Moreover, this effect may have been more pronounced in the EA-Sanyinjiao group than the EA-Guanyuan group. The underlying mechanism of the EA-induced changes may be related to gonadal hormone shifts in the HPO axis, followed by an improvement in spatial learning and memory.展开更多
目的观察艾灸关元穴对自然衰老雌性KM小鼠衰老相关行为学、肠道菌群及沉默信息调节因子1(silent information regulator of transcription 1,SIRT1)相关通路蛋白的影响。方法将16只SPF级78周龄雌性KM小鼠随机分为保健灸组和对照组,每组...目的观察艾灸关元穴对自然衰老雌性KM小鼠衰老相关行为学、肠道菌群及沉默信息调节因子1(silent information regulator of transcription 1,SIRT1)相关通路蛋白的影响。方法将16只SPF级78周龄雌性KM小鼠随机分为保健灸组和对照组,每组8只。保健灸组予温和灸关元穴,每次10分钟,隔日一次,持续30次;对照组对应放入固定器中固定不干预。干预结束后进行转棒疲劳实验和Morris水迷宫实验,搜集新鲜粪便进行肠道菌群16S rRNA高通量测序。每组随机抽取3只小鼠取材,免疫组化法检测海马组织中SIRT1、抑癌蛋白p53(tumor protein p53,P53)、过氧化物酶体增殖物激活受体α(peroxisome proliferator-activated receptor-α,PPAR-α)、过氧化物酶体增殖物激活受体γ(peroxisome proliferator-activated receptor-γ,PPAR-γ)蛋白表达。继续饲养其余小鼠至全部死亡,记录生存期。结果与对照组相比,保健灸组转棒时间无明显差异(P>0.05),水迷宫实验目标象限潜伏期延长(P<0.05);保健灸组肠道菌群多样性增加,毛螺菌科(Lachnoclostridium)、罗氏菌属(Roseburia)、另支菌属(Alistipes)等显著升高;SIRT1表达量增加(P<0.05),P53表达量降低(P<0.05),PPAR-α、PPAR-γ无明显差异(P>0.05)。两组大鼠生存期无明显差异(P>0.05)。结论艾灸关元穴可以提升小鼠学习记忆能力,改善肠道菌群结构,可能通过上调SIRT1的表达,抑制P53介导的细胞凋亡达到改善衰老状态的目的。展开更多
基金the National Science Foundation of China(the Experimental Research on Acupuncture for Prevention and Treatment Alzheimer's Disease Based on the Adjustment Imbalances of NIM,No.81072768)
文摘OBJECTIVE: To investigate the effects of electroacupuncture(EA) at the Guanyuan(CV 4) or Sanyinjiao(SP 6) acupoints on the hypothalamus-pituitary-ovary(HPO) axis and spatial learning and memory in female mice.METHODS: Nine-month-old female mice with senescence-accelerated mouse prone 8(SAMP8)were divided into three groups: the disease model,EA-Guanyuan and EA-Sanyinjiao groups. Concurrently, 9-month old female mice with senescence-accelerated mouse resistance 1(SAMR1)were set as the control model group. The two treatment groups were given the same pattern of EA stimulation. Gonadotropin-releasing hormone, luteinizing hormone, follicle-stimulating hormone(FSH) and Serum estradiol levels in the Hypothalamus-pituitary-ovary axis were assessed by enzyme-linked immunosorbent assay to determinethe HPO axis function level. Spatial learning and memory were assessed by the Morris Water Maze(MWM) test.RESULTS:(a) HPO axis: compared with the control model group, the disease model group displayed a decrease in E2 levels(P < 0.01), and an increase in Gn RH, LH and FSH levels(P < 0.01). E2 levels were increased in EA treatment groups compared with the disease model group(P < 0.05). In contrast,Gn RH and LH and FSH levels were reduced(P <0.05). EA-Sanyinjiao group was superior than EA-Guanyuan group on increasing E2 and declining Gn RH levels(P < 0.01).(b) The MWM test demonstrated that the response latency in the EA-Sanyinjiao treatment group declined from day 2 to day5 compared with the disease model group(P <0.05), whereas the EA-Guanyuan treatment group showed no significant difference.CONCLUSION: EA can regulate hormone(E2, FSH,LH, Gn RH) levels in the HPO axis and the spatial learning and memory ability in female SAMP8 mice. Moreover, this effect may have been more pronounced in the EA-Sanyinjiao group than the EA-Guanyuan group. The underlying mechanism of the EA-induced changes may be related to gonadal hormone shifts in the HPO axis, followed by an improvement in spatial learning and memory.
文摘目的观察艾灸关元穴对自然衰老雌性KM小鼠衰老相关行为学、肠道菌群及沉默信息调节因子1(silent information regulator of transcription 1,SIRT1)相关通路蛋白的影响。方法将16只SPF级78周龄雌性KM小鼠随机分为保健灸组和对照组,每组8只。保健灸组予温和灸关元穴,每次10分钟,隔日一次,持续30次;对照组对应放入固定器中固定不干预。干预结束后进行转棒疲劳实验和Morris水迷宫实验,搜集新鲜粪便进行肠道菌群16S rRNA高通量测序。每组随机抽取3只小鼠取材,免疫组化法检测海马组织中SIRT1、抑癌蛋白p53(tumor protein p53,P53)、过氧化物酶体增殖物激活受体α(peroxisome proliferator-activated receptor-α,PPAR-α)、过氧化物酶体增殖物激活受体γ(peroxisome proliferator-activated receptor-γ,PPAR-γ)蛋白表达。继续饲养其余小鼠至全部死亡,记录生存期。结果与对照组相比,保健灸组转棒时间无明显差异(P>0.05),水迷宫实验目标象限潜伏期延长(P<0.05);保健灸组肠道菌群多样性增加,毛螺菌科(Lachnoclostridium)、罗氏菌属(Roseburia)、另支菌属(Alistipes)等显著升高;SIRT1表达量增加(P<0.05),P53表达量降低(P<0.05),PPAR-α、PPAR-γ无明显差异(P>0.05)。两组大鼠生存期无明显差异(P>0.05)。结论艾灸关元穴可以提升小鼠学习记忆能力,改善肠道菌群结构,可能通过上调SIRT1的表达,抑制P53介导的细胞凋亡达到改善衰老状态的目的。
