Root and shoot growth of Brassica campestris L. and B. juncea increased significantly （P 〈 0.01） with enhanced Ca2＋ treatment along with 60 mM NaCl in the root medium. The maximum fresh mass of shoot and root in B...Root and shoot growth of Brassica campestris L. and B. juncea increased significantly （P 〈 0.01） with enhanced Ca2＋ treatment along with 60 mM NaCl in the root medium. The maximum fresh mass of shoot and root in B.juncea was recorded at 10 mM Ca2＋ concentration. The relative growth rate of shoot of both species reached its maximum at 8 mM of Ca2＋ concentration. Average rate of Ca2＋ intake （lCa） was higher in B. juncea than 6. campestris. In B. juncea, the average transport of Ca2＋ to shoot increased by 19%, 38%, 119%, 125% and 169% compared with the control. Furthermore specific utilization rate of Ca2＋ was higher in B. juncea than B. campestris. In B. campestris it increased by 9%, 32%, 41% and 59% at 4, 6, 8, and 10 mM of calcium in comparison to 2 mM Ca2＋ treatment. At 4, 6, 8 and 10 mM of Ca2＋ application, the increase in the leaf area ratio was 10,17, 23 and 30%, respectively. In the shoot and root portions of B. campestris and B.juncea, Ca2＋ had a linear relationship with potassium and sulfur, whereas it was in antagonism with sodium ion.展开更多
以茎瘤芥(Brassica juncea Coss.var.tumida Tsen et Lee)黑斑病病株为试材,对黑斑病菌5.8 S rDNA及其侧翼ITS区序列进行克隆、测序和比对分析。结果表明,5个供试病菌碱基序列同芸薹链格孢的碱基序列相似度达到99.68%,不存在大于3 bp的...以茎瘤芥(Brassica juncea Coss.var.tumida Tsen et Lee)黑斑病病株为试材,对黑斑病菌5.8 S rDNA及其侧翼ITS区序列进行克隆、测序和比对分析。结果表明,5个供试病菌碱基序列同芸薹链格孢的碱基序列相似度达到99.68%,不存在大于3 bp的碱基差异;而与甘蓝链格孢和萝卜链格孢的碱基差异较明显,均存在大于3 bp的碱基差异,且存在大量的缺失片段。初步确定引起茎瘤芥黑斑病的病原菌为芸薹链格孢。展开更多
为了深入研究调控芥菜开花信号整合子的两个核心转录因子Flowering Locus C(FLC)和SHOTR VEGETATIVE PHASE(SVP)的作用机理和进行人工调控,以芥菜‘QJ’为材料,采用RT-PCR技术分别扩增FLC和SVP的编码序列,构建原核表达质粒pET43.1a-FLC...为了深入研究调控芥菜开花信号整合子的两个核心转录因子Flowering Locus C(FLC)和SHOTR VEGETATIVE PHASE(SVP)的作用机理和进行人工调控,以芥菜‘QJ’为材料,采用RT-PCR技术分别扩增FLC和SVP的编码序列,构建原核表达质粒pET43.1a-FLC、pET43.1a-SVP,转化宿主菌大肠杆菌BL21,通过SDS-PAGE检测该蛋白的表达。利用免疫共沉淀原理及pET43.1a-FLC、pET43.1a-SVP融合蛋白序列中的6×His标签能与Ni+结合的特点,对芥菜FLC与SVP的相互作用进行SDS-PAGE检测。结果表明:FLC与SVP能在体外相互作用并形成复合体,为深入分析FLC与SVP间的作用机理以及探讨其与下游开花信号整合子元件的相互作用提供了理论和技术基础。展开更多
文摘Root and shoot growth of Brassica campestris L. and B. juncea increased significantly （P 〈 0.01） with enhanced Ca2＋ treatment along with 60 mM NaCl in the root medium. The maximum fresh mass of shoot and root in B.juncea was recorded at 10 mM Ca2＋ concentration. The relative growth rate of shoot of both species reached its maximum at 8 mM of Ca2＋ concentration. Average rate of Ca2＋ intake （lCa） was higher in B. juncea than 6. campestris. In B. juncea, the average transport of Ca2＋ to shoot increased by 19%, 38%, 119%, 125% and 169% compared with the control. Furthermore specific utilization rate of Ca2＋ was higher in B. juncea than B. campestris. In B. campestris it increased by 9%, 32%, 41% and 59% at 4, 6, 8, and 10 mM of calcium in comparison to 2 mM Ca2＋ treatment. At 4, 6, 8 and 10 mM of Ca2＋ application, the increase in the leaf area ratio was 10,17, 23 and 30%, respectively. In the shoot and root portions of B. campestris and B.juncea, Ca2＋ had a linear relationship with potassium and sulfur, whereas it was in antagonism with sodium ion.
文摘以茎瘤芥(Brassica juncea Coss.var.tumida Tsen et Lee)黑斑病病株为试材,对黑斑病菌5.8 S rDNA及其侧翼ITS区序列进行克隆、测序和比对分析。结果表明,5个供试病菌碱基序列同芸薹链格孢的碱基序列相似度达到99.68%,不存在大于3 bp的碱基差异;而与甘蓝链格孢和萝卜链格孢的碱基差异较明显,均存在大于3 bp的碱基差异,且存在大量的缺失片段。初步确定引起茎瘤芥黑斑病的病原菌为芸薹链格孢。
文摘为了深入研究调控芥菜开花信号整合子的两个核心转录因子Flowering Locus C(FLC)和SHOTR VEGETATIVE PHASE(SVP)的作用机理和进行人工调控,以芥菜‘QJ’为材料,采用RT-PCR技术分别扩增FLC和SVP的编码序列,构建原核表达质粒pET43.1a-FLC、pET43.1a-SVP,转化宿主菌大肠杆菌BL21,通过SDS-PAGE检测该蛋白的表达。利用免疫共沉淀原理及pET43.1a-FLC、pET43.1a-SVP融合蛋白序列中的6×His标签能与Ni+结合的特点,对芥菜FLC与SVP的相互作用进行SDS-PAGE检测。结果表明:FLC与SVP能在体外相互作用并形成复合体,为深入分析FLC与SVP间的作用机理以及探讨其与下游开花信号整合子元件的相互作用提供了理论和技术基础。
