Established in 2019,the Shandong Port Group(SPG)comprises four port groups(Qingdao Port,Rizhao Port,Yantai Port,and Bohaiwan Port)and 12 business segments.SPG connects 3,345 kilometers of coastline within Shandong Pro...Established in 2019,the Shandong Port Group(SPG)comprises four port groups(Qingdao Port,Rizhao Port,Yantai Port,and Bohaiwan Port)and 12 business segments.SPG connects 3,345 kilometers of coastline within Shandong Province.Its cargo throughput has consistently ranked first globally for many years,and its container volume growth ranks second globally,forming a port cluster covering the entire industrial chain.展开更多
Alzheimer’s disease(AD)is the most common form of dementia characterized pathologically by the deposition of amyloid plaques and hyperphosphorylated tau containing neurofibrillary tangles.The disease presents clinica...Alzheimer’s disease(AD)is the most common form of dementia characterized pathologically by the deposition of amyloid plaques and hyperphosphorylated tau containing neurofibrillary tangles.The disease presents clinically with progressive memory loss and disruption of cognitive function.Currently,there is no cure for AD;recent advances in the therapeutics aimed at clearing the amyloid protein from the brain have led to potential disease stabilization,however,this does not prevent eventual disease progression(Cummings et al.,2024).展开更多
Objective:Triple-negative breast cancer(TNBC)is highly aggressive and lacks an effective targeted therapy.This study aimed to elucidate the functions and possible mechanisms of action of zinc finger miz-type containin...Objective:Triple-negative breast cancer(TNBC)is highly aggressive and lacks an effective targeted therapy.This study aimed to elucidate the functions and possible mechanisms of action of zinc finger miz-type containing 2(ZMIZ2)and minichromosome maintenance complex component 3(MCM3)in TNBC progression.Methods:The relationship between ZMIZ2 expression and clinical characteristics of TNBC was investigated.In vitro and in vivo experiments were performed to investigate the role of ZMIZ2 dysregulation in TNBC cell malignant behaviors.The regulatory relationship between ZMIZ2 and MCM3 was also explored.Transcriptome sequencing was performed to elucidate possible mechanisms underlying the ZMIZ2/MCM3 axis in TNBC.Results:High ZMIZ2 expression levels were associated with the malignant degree of TNBC.ZMIZ2 overexpression promoted TNBC cell proliferation,migration,and invasion;inhibited apoptosis;and induced G1 phase cell cycle arrest,whereas knockdown of ZMIZ2 had the opposite effect.ZMIZ2 directly targeted and positively regulated MCM3 expression.MCM3 knockdown reversed the effect of ZMIZ2 overexpression on TNBC tumor growth both in vitro and in vivo.High MCM3 expression levels were linked to the degree of malignancy and poor prognosis in TNBC.The differentially expressed genes associated with the ZMIZ2/MCM3 axis were significantly enriched in multiple pathways,such as the mitogen-activated protein kinase(MAPK),mechanistic target of rapamycin(mTOR),Wnt,and Ras signaling pathways,as verified by The Cancer Genome Atlas data.Conclusions:ZMIZ2 and MCM3 were highly expressed in TNBC.ZMIZ2 promoted the development by positively regulating MCM3 expression.Key pathways,such as the Ras/MAPK,phosphatidylinositol 3-kinase(PI3K)/protein kinase B(AKT)/mTOR,and Wnt signaling pathways,may be key downstreammechanisms.展开更多
Mesenchymal stem cells(MSCs),which are mechanosensitive cells,mediate the cells crosstalk in response to mechanical force,thereby playing a crucial role in bone homeostasis.Migrasomes serve as an important mediator fo...Mesenchymal stem cells(MSCs),which are mechanosensitive cells,mediate the cells crosstalk in response to mechanical force,thereby playing a crucial role in bone homeostasis.Migrasomes serve as an important mediator for cellular communication.However,whether the mechanical stimulus regulates the biology and property of migrasomes on bone metabolism remains unknown.This study shows that mechanical stimulus could promote MSCs to synthesize and secrete migrasomes,which could significantly alleviate chronic infectious bone destruction in periodontal tissue by inhibiting osteoclastic differentiation of macrophage and reestablishing local immune microenvironment.Mechanistically,miR-29b-3p is more abundant in migrasomes from mechanical force stimulated MSCs than in control ones.MiR-29b-3p blocks the activation of pyrin domain containing protein 3(NLRP3)and mitochondrial DNA(mtDNA)release by directly targeting on Tet1.Thus,mechanical sensing migrasomes inhibit osteoclast differentiation to alleviate inflammation induced bone destruction.These findings reveal that the mechanical stimulus controls the formation and properties of migrasomes,which provides a new biotechnological strategy for chronic infectious bone destruction intervention.展开更多
Recently,Tongling Nonferrous Metals Group Co.,Ltd.issued its'Social Responsibility Report 2017'.In 2017,Tongling Nonferrous Metals actively responded to the grim and complicated international and domestic situ...Recently,Tongling Nonferrous Metals Group Co.,Ltd.issued its'Social Responsibility Report 2017'.In 2017,Tongling Nonferrous Metals actively responded to the grim and complicated international and domestic situations,and made every effort to overcome difficulties such as rising rigidity of production costs and increasing pressure on safety展开更多
The ongoing development of small molecule drugs underscores the urgent need for novel excipients to formulate poorly soluble drug candidates.Cucurbit[7]uril(CB[7])possesses high binding affinities for a variety of mol...The ongoing development of small molecule drugs underscores the urgent need for novel excipients to formulate poorly soluble drug candidates.Cucurbit[7]uril(CB[7])possesses high binding affinities for a variety of molecular vips.However,its moderate water solubility limits broader application.Here we report the synthesis of three CB[7]derivatives M1-M3 by modifying an average of 4.2,5.5,and 5.9 sulfonatopropoxy groups onto their"equator"carbons.Compared to CB[7],their water-solubility increased by at least 26.6-,23.6-,and 19.2-fold,respectively,while the maximum tolerated doses(MTD)of M1 and M2 improved by 2.5-and 2.3-fold.Phase solubility diagram studies demonstrate that M1 and M2 significantly enhance the water-solubility of eighteen poorly soluble drugs.In vivo experiments in rat complete Freund's arthritis reveal that M1 not only improves the anti-inflammatory efficacy of indomethacin by up to 52%,but also substantially reduces its side effect of gastric ulcer.展开更多
Maize(Zea mays),which is a vital source of food,feed,and energy feedstock globally,has significant potential for higher yields.However,environmental stress conditions,including drought and salt stress,severely restric...Maize(Zea mays),which is a vital source of food,feed,and energy feedstock globally,has significant potential for higher yields.However,environmental stress conditions,including drought and salt stress,severely restrict maize plant growth and development,leading to great yield losses.Leucine-rich repeat receptor-like kinases(LRR-RLKs)function in biotic and abiotic stress responses in the model plant Arabidopsis(Arabidopsis thaliana),but their roles in abiotic stress responses in maize are not entirely understood.In this study,we determine that the LRR-RLK ZmMIK2,a homolog of the Arabidopsis LRR-RK MALE DISCOVERER 1(MDIS1)-INTERACTING RECEPTOR LIKE KINASE 2(MIK2),functions in resistance to both drought and salt stress in maize.Zmmik2 plants exhibit enhanced resistance to both stresses,whereas overexpressing ZmMIK2 confers the opposite phenotypes.Furthermore,we identify C2-DOMAIN-CONTAINING PROTEIN 1(ZmC2DP1),which interacts with the intracellular region of ZmMIK2.Notably,that region of ZmMIK2 mediates the phosphorylation of ZmC2DP1,likely by increasing its stability.Both ZmMIK2 and ZmC2DP1 are mainly expressed in roots.As with ZmMIK2,knockout of ZmC2DP1 enhances resistance to both drought and salt stress.We conclude that ZmMIK2-ZmC2DP1 acts as a negative regulatory module in maize drought-and salt-stress responses.展开更多
The cavity characteristics in liquid-filled containers caused by high-velocity impacts represent an important area of research in hydrodynamic ram phenomena.The dynamic expansion of the cavity induces liquid pressure ...The cavity characteristics in liquid-filled containers caused by high-velocity impacts represent an important area of research in hydrodynamic ram phenomena.The dynamic expansion of the cavity induces liquid pressure variations,potentially causing catastrophic damage to the container.Current studies mainly focus on non-deforming projectiles,such as fragments,with limited exploration of shaped charge jets.In this paper,a uniquely experimental system was designed to record cavity profiles in behind-armor liquid-filled containers subjected to shaped charge jet impacts.The impact process was then numerically reproduced using the explicit simulation program ANSYS LS-DYNA with the Structured Arbitrary Lagrangian-Eulerian(S-ALE)solver.The formation mechanism,along with the dimensional and shape evolution of the cavity was investigated.Additionally,the influence of the impact kinetic energy of the jet on the cavity characteristics was analyzed.The findings reveal that the cavity profile exhibits a conical shape,primarily driven by direct jet impact and inertial effects.The expansion rates of both cavity length and maximum radius increase with jet impact kinetic energy.When the impact kinetic energy is reduced to 28.2 kJ or below,the length-to-diameter ratio of the cavity ultimately stabilizes at approximately 7.展开更多
The functional and structural integrity of the blood-brain barrier is crucial in maintaining homeostasis in the brain microenvironment;however,the molecular mechanisms underlying the formation and function of the bloo...The functional and structural integrity of the blood-brain barrier is crucial in maintaining homeostasis in the brain microenvironment;however,the molecular mechanisms underlying the formation and function of the blood-brain barrier remain poorly understood.The major facilitator superfamily domain containing 2A has been identified as a key regulator of blood-brain barrier function.It plays a critical role in promoting and maintaining the formation and functional stability of the blood-brain barrier,in addition to the transport of lipids,such as docosahexaenoic acid,across the blood-brain barrier.Furthermore,an increasing number of studies have suggested that major facilitator superfamily domain containing 2A is involved in the molecular mechanisms of blood-brain barrier dysfunction in a variety of neurological diseases;however,little is known regarding the mechanisms by which major facilitator superfamily domain containing 2A affects the blood-brain barrier.This paper provides a comprehensive and systematic review of the close relationship between major facilitator superfamily domain containing 2A proteins and the blood-brain barrier,including their basic structures and functions,cross-linking between major facilitator superfamily domain containing 2A and the blood-brain barrier,and the in-depth studies on lipid transport and the regulation of blood-brain barrier permeability.This comprehensive systematic review contributes to an in-depth understanding of the important role of major facilitator superfamily domain containing 2A proteins in maintaining the structure and function of the blood-brain barrier and the research progress to date.This will not only help to elucidate the pathogenesis of neurological diseases,improve the accuracy of laboratory diagnosis,and optimize clinical treatment strategies,but it may also play an important role in prognostic monitoring.In addition,the effects of major facilitator superfamily domain containing 2A on blood-brain barrier leakage in various diseases and the research progress on cross-blood-brain barrier drug delivery are summarized.This review may contribute to the development of new approaches for the treatment of neurological diseases.展开更多
Kubernetes has become the dominant container orchestration platform,withwidespread adoption across industries.However,its default pod-to-pod communicationmechanism introduces security vulnerabilities,particularly IP s...Kubernetes has become the dominant container orchestration platform,withwidespread adoption across industries.However,its default pod-to-pod communicationmechanism introduces security vulnerabilities,particularly IP spoofing attacks.Attackers can exploit this weakness to impersonate legitimate pods,enabling unauthorized access,lateral movement,and large-scale Distributed Denial of Service(DDoS)attacks.Existing security mechanisms such as network policies and intrusion detection systems introduce latency and performance overhead,making them less effective in dynamic Kubernetes environments.This research presents PodCA,an eBPF-based security framework designed to detect and prevent IP spoofing in real time while minimizing performance impact.PodCA integrates with Kubernetes’Container Network Interface(CNI)and uses eBPF to monitor and validate packet metadata at the kernel level.It maintains a container network mapping table that tracks pod IP assignments,validates packet legitimacy before forwarding,and ensures network integrity.If an attack is detected,PodCA automatically blocks spoofed packets and,in cases of repeated attempts,terminates compromised pods to prevent further exploitation.Experimental evaluation on an AWS Kubernetes cluster demonstrates that PodCA detects and prevents spoofed packets with 100%accuracy.Additionally,resource consumption analysis reveals minimal overhead,with a CPU increase of only 2–3%per node and memory usage rising by 40–60 MB.These results highlight the effectiveness of eBPF in securing Kubernetes environments with low overhead,making it a scalable and efficient security solution for containerized applications.展开更多
Biodiversity has always been valued by ecology,Diversity is generally believed to lead to stability,and biodiversity is an important condition for ecosystems to maintain health.So what factors are related to forest ec...Biodiversity has always been valued by ecology,Diversity is generally believed to lead to stability,and biodiversity is an important condition for ecosystems to maintain health.So what factors are related to forest ecosystem biodiversity,and what kind of forest structure affects and determines the size of biodiversity.This study proposes the concept of contained uniformity based on the theory of uniformity,and uses the convergence of contained uniformity to obtain the judgment model of the forest station pattern type.At the same time,it proposes the concept of forest ecosystem distance diversity,and uses the judgment model of the stand pattern type to derive the mathematical definition of forest ecosystem distance diversity.Combining the ecological characteristics of different stand patterns and measurement indicators of forest ecosystem biodiversity,the connection between forest ecosystem distance diversity and biodiversity is derived,and this is used as an indicator to evaluate forest ecosystem biodiversity.Forest ecosystem distance diversity,as an indicator of biodiversity,can not only conduct early assessment and prediction of the biodiversity of immature forests(forests in the early succession or recovery stage),but also provide a quantitative basis for forest structure optimization.Ultimately realize the sustainable development of forestry production and operation and biodiversity protection.展开更多
The population growth in Ghana has assumed an alarming rate.The provision of urban infrastructure and housing has however not been commensurate with the demand especially in housing,thus the acute housing deficit.The ...The population growth in Ghana has assumed an alarming rate.The provision of urban infrastructure and housing has however not been commensurate with the demand especially in housing,thus the acute housing deficit.The idea of using shipping containers as a building component is by no means new in the Accra Metropolis as most shipping containers are re-constructed architecturally and used for temporary accommodation needs like storage,make-shift shops,emergency shelters and site offices.The concept of using these shipping containers as modular building components in architecture however,is still foreign to building practitioners and the nation at large.This research paper set out to use the containers not for luxury apartments but to harness the merits of availability,low-cost of resource,speed of construction and structural stability of the International Standard Organization(ISO)shipping containers in addressing the housing deficiency problem in the nation by meeting the basic need of shelter.Based on the hypothesis of being a cheaper alternative to the concrete and sand-crete blocks,which is the main construction technology used now,similar house types of both technologies were compared to ascertain the variation as part of the methodology for this research.The methodology also included literature reviews and case studies.The scope of this study was limited to 2-bedroom single-storey and multi-storey house types in Accra,the capital city of Ghana.In the final analysis however,this research proved that the container house is not cheaper than the traditional blockwork and concrete construction method is and is better used in temporary accommodation,in situations where time is essential.展开更多
Formation control in multi-agent systems has become a critical area of interest due to its wide-ranging applications in robotics,autonomous transportation,and surveillance.While various studies have explored distribut...Formation control in multi-agent systems has become a critical area of interest due to its wide-ranging applications in robotics,autonomous transportation,and surveillance.While various studies have explored distributed cooperative control,this review focuses on the theoretical foundations and recent developments in formation control strategies.The paper categorizes and analyzes key formation types,including formation maintenance,group or cluster formation,bipartite formations,event-triggered formations,finite-time convergence,and constrained formations.A significant portion of the review addresses formation control under constrained dynamics,presenting both modelbased and model-free approaches that consider practical limitations such as actuator bounds,communication delays,and nonholonomic constraints.Additionally,the paper discusses emerging trends,including the integration of eventdriven mechanisms and AI-enhanced coordination strategies.Comparative evaluations highlight the trade-offs among various methodologies regarding scalability,robustness,and real-world feasibility.Practical implementations are reviewed across diverse platforms,and the review identifies the current achievements and unresolved challenges in the field.The paper concludes by outlining promising research directions,such as adaptive control for dynamic environments,energy-efficient coordination,and using learning-based control under uncertainty.This review synthesizes the current state of the art and provides a road map for future investigation,making it a valuable reference for researchers and practitioners aiming to advance formation control in multi-agent systems.展开更多
In a cloud environment,graphics processing units(GPUs)are the primary devices used for high-performance computation.They exploit flexible resource utilization,a key advantage of cloud environments.Multiple users share...In a cloud environment,graphics processing units(GPUs)are the primary devices used for high-performance computation.They exploit flexible resource utilization,a key advantage of cloud environments.Multiple users share GPUs,which serve as coprocessors of central processing units(CPUs)and are activated only if tasks demand GPU computation.In a container environment,where resources can be shared among multiple users,GPU utilization can be increased by minimizing idle time because the tasks of many users run on a single GPU.However,unlike CPUs and memory,GPUs cannot logically multiplex their resources.Additionally,GPU memory does not support over-utilization:when it runs out,tasks will fail.Therefore,it is necessary to regulate the order of execution of concurrently running GPU tasks to avoid such task failures and to ensure equitable GPU sharing among users.In this paper,we propose a GPU task execution order management technique that controls GPU usage via time-based containers.The technique seeks to ensure equal GPU time among users in a container environment to prevent task failures.In the meantime,we use a deferred processing method to prevent GPU memory shortages when GPU tasks are executed simultaneously and to determine the execution order based on the GPU usage time.As the order of GPU tasks cannot be externally adjusted arbitrarily once the task commences,the GPU task is indirectly paused by pausing the container.In addition,as container pause/unpause status is based on the information about the available GPU memory capacity,overuse of GPU memory can be prevented at the source.As a result,the strategy can prevent task failure and the GPU tasks can be experimentally processed in appropriate order.展开更多
Liquid-filled containers(LFC)are widely used to store and transport petroleum,chemical reagents,and other resources.As an important target of military strikes and terrorist bombings,LFC are vulnerable to blast waves a...Liquid-filled containers(LFC)are widely used to store and transport petroleum,chemical reagents,and other resources.As an important target of military strikes and terrorist bombings,LFC are vulnerable to blast waves and fragments.To explore the protective effect of polyurea elastomer on LFC,the damage characteristics of polyurea coated liquid-filled container(PLFC)under the combined loading of blast shock wave and fragments were studied experimentally.The microstructure of the polyurea layer was observed by scanning electron microscopy,and the fracture and self-healing phenomena were analyzed.The simulation approach was used to explain the combined blast-and fragments-induced on the PLFC in detail.Finally,the effects of shock wave and fragment alone and in combination on the damage of PLFC were comprehensively compared.Results showed that the polyurea reduces the perforation rate of the fragment to the LFC,and the self-healing phenomenon could also reduce the liquid loss rate inside the container.The polyurea reduces the degree of depression in the center of the LFC,resulting in a decrease in the distance between adjacent fragments penetrating the LFC,and an increase in the probability of transfixion and fracture between holes.Under the close-in blast,the detonation shock wave reached the LFC before the fragment.Polyurea does not all have an enhanced effect on the protection of LFC.The presence of internal water enhances the anti-blast performance of the container,and the hydrodynamic ram(HRAM)formed by the fragment impacting the water aggravated the plastic deformation of the container.The combined action has an enhancement effect on the deformation of the LFC.The depth of the container depression was 27%higher than that of the blast shock wave alone;thus,it cannot be simply summarized as linear superposition.展开更多
Mongolia is a landlocked country with limited infrastructure and high dependence on the Xingang Tianjin port in China for imports. This research examines the potential impacts of establishing a dry port in Zamyn-Uud, ...Mongolia is a landlocked country with limited infrastructure and high dependence on the Xingang Tianjin port in China for imports. This research examines the potential impacts of establishing a dry port in Zamyn-Uud, Mongolia, utilizing a system dynamics modeling approach via Vensim software. The study evaluates transportation time, costs, inflation, and logistics performance index improvements, revealing that the establishment of the dry port can reduce transportation costs and delays significantly while enhancing economic growth. The findings offer actionable insights for policymakers and stakeholders in addressing logistical inefficiencies and fostering sustainable development in landlocked regions.展开更多
Compacted sand-bentonite mixtures are used as waste containment barriers(e.g.landfill liners and bentonite buffers for nuclear waste)to restrict contaminant transport.The potential for enhanced chemical containment of...Compacted sand-bentonite mixtures are used as waste containment barriers(e.g.landfill liners and bentonite buffers for nuclear waste)to restrict contaminant transport.The potential for enhanced chemical containment of compacted sand-bentonite mixtures due to semipermeable membrane behavior has also been demonstrated.However,the extent to which membrane behavior persists in the presence of highly concentrated chemical solutions,which have been shown to degrade membrane behavior in bentonite-based barriers,remains largely unknown.Moreover,the limiting(threshold)salt concentrations at which membrane behavior of compacted sand-bentonite mixtures is effectively destroyed have not been evaluated.Accordingly,this study quantified the limiting membrane behavior of two duplicated specimens of compacted sand-bentonite mixture comprising 15%sodium bentonite(by dry mass)by determining the limiting salt concentrations at which measurable membrane behavior was eliminated.The specimens were exposed to increasingly higher source concentrations,Cot,of boundary monovalent salt solutions(KCl and NaCl)until measured values of the membrane efficiency coefficient,ω,were effectively zero.Overall,ωdecreased from an average of 0.032 to zero as Cot increased from 160 mmol/L KCl to 3.27 mol/L NaCl,resulting in limiting threshold salt concentrations for the two tests between 1.63 mol/L and 3.27 mol/L NaCl,which are significantly higher than those at which measurable membrane behavior has previously been demonstrated.展开更多
Virtualization is an indispensable part of the cloud for the objective of deploying different virtual servers over the same physical layer.However,the increase in the number of applications executing on the repositori...Virtualization is an indispensable part of the cloud for the objective of deploying different virtual servers over the same physical layer.However,the increase in the number of applications executing on the repositories results in increased overload due to the adoption of cloud services.Moreover,the migration of applications on the cloud with optimized resource allocation is a herculean task even though it is employed for minimizing the dilemma of allocating resources.In this paper,a Fire Hawk Optimization enabled Deep Learning Scheme(FHOEDLS)is proposed for minimizing the overload and optimizing the resource allocation on the hybrid cloud container architecture for migrating interoperability based applications This FHOEDLS achieves the load prediction through the utilization of deep CNN-GRU-AM model for attaining resource allocation and better migration of applications.It specifically adopted the Fire Hawk Optimization Algorithm(FHOA)for optimizing the parameters that influence the factors that aid in better interoperable application migration with improved resource allocation and minimized overhead.It considered the factors of resource capacity,transmission cost,demand,and predicted load into account during the formulation of the objective function utilized for resource allocation and application migration.The cloud simulation of this FHOEDLS is achieved using a container,Virtual Machine(VM),and Physical Machine(PM).The results of this proposed FHOEDLS confirmed a better resource capability of 0.418 and a minimized load of 0.0061.展开更多
Unlike other major crops,little research has been performed on tomato to reduce the generation time for speed breeding.We evaluated several agronomic treatments to reduce the generation time of tomato in the‘M82'...Unlike other major crops,little research has been performed on tomato to reduce the generation time for speed breeding.We evaluated several agronomic treatments to reduce the generation time of tomato in the‘M82'(determinate)and‘Moneymaker'(indeterminate)varieties and evaluated the best combination in conjunction with embryo rescue.Five container sizes with volumes of 0.2 L(XS),0.45 L(S),0.8 L(M),1.3 L(L),and6 L(XL),were evaluated in the first experiment under the autumn cycle.We found that plants grown in XL containers exhibited better development and required less time from sowing to anthesis(DSA)and from anthesis to fruit ripening(DAR).In the second experiment,using XL containers in the autumn-winter cycle,we evaluated the effects of cold priming at the cotyledonary stage,water stress,P supplementation,and K supplementation on generation time.Compared to the control,we found that cold priming significantly reduced the number of leaves,plant height to first the inflorescence,and DSA(2.7 d),whereas K supplementation reduced the DAR(8.8 d).In contrast,water stress and P supplementation did not significantly affect any of the measured traits like DAR,DSA or fruit set.To validate these data,in a third experiment with XL containers in the spring-summer cycle,the combination of cold priming and K supplementation was tested,confirming the significant effect of this combination on the reduction of generation time(2.9 d for DSA and 3.9 d for DAR)compared to the control.Embryo rescue during the cell expansion cycle(average of 22.0 d and 23.3 d after anthesis for‘M82'and‘Moneymaker',respectively)allowed the shortening of the generation time by 8.7 d in‘M82'and 11.6 d in‘Moneymaker'compared to the in planta fruit ripening.The combination of agronomic treatments with embryo rescue can effectively increase the number of generations per year from three to four for speed breeding of tomato.展开更多
文摘Established in 2019,the Shandong Port Group(SPG)comprises four port groups(Qingdao Port,Rizhao Port,Yantai Port,and Bohaiwan Port)and 12 business segments.SPG connects 3,345 kilometers of coastline within Shandong Province.Its cargo throughput has consistently ranked first globally for many years,and its container volume growth ranks second globally,forming a port cluster covering the entire industrial chain.
基金funded by Wellcome 4ward North(Ref:216340/Z/19/Z)ARUK Yorkshire Network Centre Small Grant Scheme,ARUK Preparatory Clinical Fellowship scheme(Ref:ARUK-PCRF2016A-1)+3 种基金Academy of Medical Sciences Starter Grants for Clinical Lecturers Scheme(Ref:SGL028\1097),Parkinson’s UK(Ref:F1301)Michael J Fox Foundation(Ref:005021),Australian Research Council(CE200100012)European Union Seventh Framework Programme(Ref:FP7/2007-2013)under grant agreement no.601055the NIHR Sheffield Biomedical Research Centre award(NIHR 203321)(to SMB).
文摘Alzheimer’s disease(AD)is the most common form of dementia characterized pathologically by the deposition of amyloid plaques and hyperphosphorylated tau containing neurofibrillary tangles.The disease presents clinically with progressive memory loss and disruption of cognitive function.Currently,there is no cure for AD;recent advances in the therapeutics aimed at clearing the amyloid protein from the brain have led to potential disease stabilization,however,this does not prevent eventual disease progression(Cummings et al.,2024).
基金supported by the Jilin Province Health Science and Technology Ability Improvement Project(2023JL057).
文摘Objective:Triple-negative breast cancer(TNBC)is highly aggressive and lacks an effective targeted therapy.This study aimed to elucidate the functions and possible mechanisms of action of zinc finger miz-type containing 2(ZMIZ2)and minichromosome maintenance complex component 3(MCM3)in TNBC progression.Methods:The relationship between ZMIZ2 expression and clinical characteristics of TNBC was investigated.In vitro and in vivo experiments were performed to investigate the role of ZMIZ2 dysregulation in TNBC cell malignant behaviors.The regulatory relationship between ZMIZ2 and MCM3 was also explored.Transcriptome sequencing was performed to elucidate possible mechanisms underlying the ZMIZ2/MCM3 axis in TNBC.Results:High ZMIZ2 expression levels were associated with the malignant degree of TNBC.ZMIZ2 overexpression promoted TNBC cell proliferation,migration,and invasion;inhibited apoptosis;and induced G1 phase cell cycle arrest,whereas knockdown of ZMIZ2 had the opposite effect.ZMIZ2 directly targeted and positively regulated MCM3 expression.MCM3 knockdown reversed the effect of ZMIZ2 overexpression on TNBC tumor growth both in vitro and in vivo.High MCM3 expression levels were linked to the degree of malignancy and poor prognosis in TNBC.The differentially expressed genes associated with the ZMIZ2/MCM3 axis were significantly enriched in multiple pathways,such as the mitogen-activated protein kinase(MAPK),mechanistic target of rapamycin(mTOR),Wnt,and Ras signaling pathways,as verified by The Cancer Genome Atlas data.Conclusions:ZMIZ2 and MCM3 were highly expressed in TNBC.ZMIZ2 promoted the development by positively regulating MCM3 expression.Key pathways,such as the Ras/MAPK,phosphatidylinositol 3-kinase(PI3K)/protein kinase B(AKT)/mTOR,and Wnt signaling pathways,may be key downstreammechanisms.
基金supported by the National Key Research and Development Program of China(No.2022YFA1105800(R.L.Y.))Clinical Medicine Plus X-Young Scholars Project of Peking University(No.2025PKULCXQ015(R.L.Y.))+2 种基金Research Foundation of Peking University School and Hospital of Stomatology(No.PKUSS20230103)the Fundamental Research Funds for the Central Universities-Peking University Clinical Scientist Training Program(No.L232107(R.L.Y.))Beijing Municipal Natural Science Foundation-Haidian Original Innovation Joint Fund(Nos.L222001(Xiaomo Liu)and L232107(Jie Shi)).
文摘Mesenchymal stem cells(MSCs),which are mechanosensitive cells,mediate the cells crosstalk in response to mechanical force,thereby playing a crucial role in bone homeostasis.Migrasomes serve as an important mediator for cellular communication.However,whether the mechanical stimulus regulates the biology and property of migrasomes on bone metabolism remains unknown.This study shows that mechanical stimulus could promote MSCs to synthesize and secrete migrasomes,which could significantly alleviate chronic infectious bone destruction in periodontal tissue by inhibiting osteoclastic differentiation of macrophage and reestablishing local immune microenvironment.Mechanistically,miR-29b-3p is more abundant in migrasomes from mechanical force stimulated MSCs than in control ones.MiR-29b-3p blocks the activation of pyrin domain containing protein 3(NLRP3)and mitochondrial DNA(mtDNA)release by directly targeting on Tet1.Thus,mechanical sensing migrasomes inhibit osteoclast differentiation to alleviate inflammation induced bone destruction.These findings reveal that the mechanical stimulus controls the formation and properties of migrasomes,which provides a new biotechnological strategy for chronic infectious bone destruction intervention.
文摘Recently,Tongling Nonferrous Metals Group Co.,Ltd.issued its'Social Responsibility Report 2017'.In 2017,Tongling Nonferrous Metals actively responded to the grim and complicated international and domestic situations,and made every effort to overcome difficulties such as rising rigidity of production costs and increasing pressure on safety
基金National Natural Science Foundation of China(Nos.21921003 and 22201293)the National Key R&D Program of China(No.2023YFC3503400)for financial support。
文摘The ongoing development of small molecule drugs underscores the urgent need for novel excipients to formulate poorly soluble drug candidates.Cucurbit[7]uril(CB[7])possesses high binding affinities for a variety of molecular vips.However,its moderate water solubility limits broader application.Here we report the synthesis of three CB[7]derivatives M1-M3 by modifying an average of 4.2,5.5,and 5.9 sulfonatopropoxy groups onto their"equator"carbons.Compared to CB[7],their water-solubility increased by at least 26.6-,23.6-,and 19.2-fold,respectively,while the maximum tolerated doses(MTD)of M1 and M2 improved by 2.5-and 2.3-fold.Phase solubility diagram studies demonstrate that M1 and M2 significantly enhance the water-solubility of eighteen poorly soluble drugs.In vivo experiments in rat complete Freund's arthritis reveal that M1 not only improves the anti-inflammatory efficacy of indomethacin by up to 52%,but also substantially reduces its side effect of gastric ulcer.
基金supported by the National Key Research and Development Program of China(2021YFD1200703 and 2022YFF1001602)the National Science Foundation of China(32272024 and 32171940)+2 种基金the Pinduoduo-China Agricultural University Research Fund(PC2023B01001)the Chinese Universities Scientific Fund(2022TC142)the 2115 Talent Development Program of China Agricultural University。
文摘Maize(Zea mays),which is a vital source of food,feed,and energy feedstock globally,has significant potential for higher yields.However,environmental stress conditions,including drought and salt stress,severely restrict maize plant growth and development,leading to great yield losses.Leucine-rich repeat receptor-like kinases(LRR-RLKs)function in biotic and abiotic stress responses in the model plant Arabidopsis(Arabidopsis thaliana),but their roles in abiotic stress responses in maize are not entirely understood.In this study,we determine that the LRR-RLK ZmMIK2,a homolog of the Arabidopsis LRR-RK MALE DISCOVERER 1(MDIS1)-INTERACTING RECEPTOR LIKE KINASE 2(MIK2),functions in resistance to both drought and salt stress in maize.Zmmik2 plants exhibit enhanced resistance to both stresses,whereas overexpressing ZmMIK2 confers the opposite phenotypes.Furthermore,we identify C2-DOMAIN-CONTAINING PROTEIN 1(ZmC2DP1),which interacts with the intracellular region of ZmMIK2.Notably,that region of ZmMIK2 mediates the phosphorylation of ZmC2DP1,likely by increasing its stability.Both ZmMIK2 and ZmC2DP1 are mainly expressed in roots.As with ZmMIK2,knockout of ZmC2DP1 enhances resistance to both drought and salt stress.We conclude that ZmMIK2-ZmC2DP1 acts as a negative regulatory module in maize drought-and salt-stress responses.
基金financial support from the National Natural Science Foundation of China(Grant No.11572159).
文摘The cavity characteristics in liquid-filled containers caused by high-velocity impacts represent an important area of research in hydrodynamic ram phenomena.The dynamic expansion of the cavity induces liquid pressure variations,potentially causing catastrophic damage to the container.Current studies mainly focus on non-deforming projectiles,such as fragments,with limited exploration of shaped charge jets.In this paper,a uniquely experimental system was designed to record cavity profiles in behind-armor liquid-filled containers subjected to shaped charge jet impacts.The impact process was then numerically reproduced using the explicit simulation program ANSYS LS-DYNA with the Structured Arbitrary Lagrangian-Eulerian(S-ALE)solver.The formation mechanism,along with the dimensional and shape evolution of the cavity was investigated.Additionally,the influence of the impact kinetic energy of the jet on the cavity characteristics was analyzed.The findings reveal that the cavity profile exhibits a conical shape,primarily driven by direct jet impact and inertial effects.The expansion rates of both cavity length and maximum radius increase with jet impact kinetic energy.When the impact kinetic energy is reduced to 28.2 kJ or below,the length-to-diameter ratio of the cavity ultimately stabilizes at approximately 7.
基金supported by the National Natural Science Foundation of China,No.82104412(to TD)Shaanxi Provincial Key R&D Program,No.2023-YBSF-165(to TD)+1 种基金the Natural Science Foundation of Shaanxi Department of Science and Technology,No.2018JM7022(to FM)Shaanxi Provincial Key Industry Chain Project,No.2021ZDLSF04-11(to PW)。
文摘The functional and structural integrity of the blood-brain barrier is crucial in maintaining homeostasis in the brain microenvironment;however,the molecular mechanisms underlying the formation and function of the blood-brain barrier remain poorly understood.The major facilitator superfamily domain containing 2A has been identified as a key regulator of blood-brain barrier function.It plays a critical role in promoting and maintaining the formation and functional stability of the blood-brain barrier,in addition to the transport of lipids,such as docosahexaenoic acid,across the blood-brain barrier.Furthermore,an increasing number of studies have suggested that major facilitator superfamily domain containing 2A is involved in the molecular mechanisms of blood-brain barrier dysfunction in a variety of neurological diseases;however,little is known regarding the mechanisms by which major facilitator superfamily domain containing 2A affects the blood-brain barrier.This paper provides a comprehensive and systematic review of the close relationship between major facilitator superfamily domain containing 2A proteins and the blood-brain barrier,including their basic structures and functions,cross-linking between major facilitator superfamily domain containing 2A and the blood-brain barrier,and the in-depth studies on lipid transport and the regulation of blood-brain barrier permeability.This comprehensive systematic review contributes to an in-depth understanding of the important role of major facilitator superfamily domain containing 2A proteins in maintaining the structure and function of the blood-brain barrier and the research progress to date.This will not only help to elucidate the pathogenesis of neurological diseases,improve the accuracy of laboratory diagnosis,and optimize clinical treatment strategies,but it may also play an important role in prognostic monitoring.In addition,the effects of major facilitator superfamily domain containing 2A on blood-brain barrier leakage in various diseases and the research progress on cross-blood-brain barrier drug delivery are summarized.This review may contribute to the development of new approaches for the treatment of neurological diseases.
基金partially supported by Asia Pacific University of Technology&Innovation(APU)Bukit Jalil,Kuala Lumpur,MalaysiaThe funding body had no role in the study design,data collection,analysis,interpretation,or writing of the manuscript.
文摘Kubernetes has become the dominant container orchestration platform,withwidespread adoption across industries.However,its default pod-to-pod communicationmechanism introduces security vulnerabilities,particularly IP spoofing attacks.Attackers can exploit this weakness to impersonate legitimate pods,enabling unauthorized access,lateral movement,and large-scale Distributed Denial of Service(DDoS)attacks.Existing security mechanisms such as network policies and intrusion detection systems introduce latency and performance overhead,making them less effective in dynamic Kubernetes environments.This research presents PodCA,an eBPF-based security framework designed to detect and prevent IP spoofing in real time while minimizing performance impact.PodCA integrates with Kubernetes’Container Network Interface(CNI)and uses eBPF to monitor and validate packet metadata at the kernel level.It maintains a container network mapping table that tracks pod IP assignments,validates packet legitimacy before forwarding,and ensures network integrity.If an attack is detected,PodCA automatically blocks spoofed packets and,in cases of repeated attempts,terminates compromised pods to prevent further exploitation.Experimental evaluation on an AWS Kubernetes cluster demonstrates that PodCA detects and prevents spoofed packets with 100%accuracy.Additionally,resource consumption analysis reveals minimal overhead,with a CPU increase of only 2–3%per node and memory usage rising by 40–60 MB.These results highlight the effectiveness of eBPF in securing Kubernetes environments with low overhead,making it a scalable and efficient security solution for containerized applications.
基金funded by Research on Intelligent Control System of Variable Fertilization of Deep Application Liquid Fertilizer(GXKS2022GKY003)the Key Laboratory of Smart Agriculture Technology,Guangxi Science and Technology Normal University(GXKSKYPT2024008)+1 种基金Key Laboratory of Detection Technology for Sugarcane and Peanut Leaf Spot Disease,Guangxi Science and Technology Normal University(GXKSKYPT2025008)Research on the Application of Uniformity Theory and 3D Virtual Forest Phase in Harbin Normal University’s Science and Technology Innovation Climbing Program Funding Project(XKB202415)。
文摘Biodiversity has always been valued by ecology,Diversity is generally believed to lead to stability,and biodiversity is an important condition for ecosystems to maintain health.So what factors are related to forest ecosystem biodiversity,and what kind of forest structure affects and determines the size of biodiversity.This study proposes the concept of contained uniformity based on the theory of uniformity,and uses the convergence of contained uniformity to obtain the judgment model of the forest station pattern type.At the same time,it proposes the concept of forest ecosystem distance diversity,and uses the judgment model of the stand pattern type to derive the mathematical definition of forest ecosystem distance diversity.Combining the ecological characteristics of different stand patterns and measurement indicators of forest ecosystem biodiversity,the connection between forest ecosystem distance diversity and biodiversity is derived,and this is used as an indicator to evaluate forest ecosystem biodiversity.Forest ecosystem distance diversity,as an indicator of biodiversity,can not only conduct early assessment and prediction of the biodiversity of immature forests(forests in the early succession or recovery stage),but also provide a quantitative basis for forest structure optimization.Ultimately realize the sustainable development of forestry production and operation and biodiversity protection.
文摘The population growth in Ghana has assumed an alarming rate.The provision of urban infrastructure and housing has however not been commensurate with the demand especially in housing,thus the acute housing deficit.The idea of using shipping containers as a building component is by no means new in the Accra Metropolis as most shipping containers are re-constructed architecturally and used for temporary accommodation needs like storage,make-shift shops,emergency shelters and site offices.The concept of using these shipping containers as modular building components in architecture however,is still foreign to building practitioners and the nation at large.This research paper set out to use the containers not for luxury apartments but to harness the merits of availability,low-cost of resource,speed of construction and structural stability of the International Standard Organization(ISO)shipping containers in addressing the housing deficiency problem in the nation by meeting the basic need of shelter.Based on the hypothesis of being a cheaper alternative to the concrete and sand-crete blocks,which is the main construction technology used now,similar house types of both technologies were compared to ascertain the variation as part of the methodology for this research.The methodology also included literature reviews and case studies.The scope of this study was limited to 2-bedroom single-storey and multi-storey house types in Accra,the capital city of Ghana.In the final analysis however,this research proved that the container house is not cheaper than the traditional blockwork and concrete construction method is and is better used in temporary accommodation,in situations where time is essential.
基金supported in part by the National Natural Science Foundation of China under Grant 6237319in part by the Postgraduate Research and Practice Innovation Program of Jiangsu Province under Grant KYCX230479.
文摘Formation control in multi-agent systems has become a critical area of interest due to its wide-ranging applications in robotics,autonomous transportation,and surveillance.While various studies have explored distributed cooperative control,this review focuses on the theoretical foundations and recent developments in formation control strategies.The paper categorizes and analyzes key formation types,including formation maintenance,group or cluster formation,bipartite formations,event-triggered formations,finite-time convergence,and constrained formations.A significant portion of the review addresses formation control under constrained dynamics,presenting both modelbased and model-free approaches that consider practical limitations such as actuator bounds,communication delays,and nonholonomic constraints.Additionally,the paper discusses emerging trends,including the integration of eventdriven mechanisms and AI-enhanced coordination strategies.Comparative evaluations highlight the trade-offs among various methodologies regarding scalability,robustness,and real-world feasibility.Practical implementations are reviewed across diverse platforms,and the review identifies the current achievements and unresolved challenges in the field.The paper concludes by outlining promising research directions,such as adaptive control for dynamic environments,energy-efficient coordination,and using learning-based control under uncertainty.This review synthesizes the current state of the art and provides a road map for future investigation,making it a valuable reference for researchers and practitioners aiming to advance formation control in multi-agent systems.
基金supported by“Regional Innovation Strategy(RIS)”through the National Research Foundation of Korea(NRF)funded by the Ministry of Education(MOE)(2023RIS-009).
文摘In a cloud environment,graphics processing units(GPUs)are the primary devices used for high-performance computation.They exploit flexible resource utilization,a key advantage of cloud environments.Multiple users share GPUs,which serve as coprocessors of central processing units(CPUs)and are activated only if tasks demand GPU computation.In a container environment,where resources can be shared among multiple users,GPU utilization can be increased by minimizing idle time because the tasks of many users run on a single GPU.However,unlike CPUs and memory,GPUs cannot logically multiplex their resources.Additionally,GPU memory does not support over-utilization:when it runs out,tasks will fail.Therefore,it is necessary to regulate the order of execution of concurrently running GPU tasks to avoid such task failures and to ensure equitable GPU sharing among users.In this paper,we propose a GPU task execution order management technique that controls GPU usage via time-based containers.The technique seeks to ensure equal GPU time among users in a container environment to prevent task failures.In the meantime,we use a deferred processing method to prevent GPU memory shortages when GPU tasks are executed simultaneously and to determine the execution order based on the GPU usage time.As the order of GPU tasks cannot be externally adjusted arbitrarily once the task commences,the GPU task is indirectly paused by pausing the container.In addition,as container pause/unpause status is based on the information about the available GPU memory capacity,overuse of GPU memory can be prevented at the source.As a result,the strategy can prevent task failure and the GPU tasks can be experimentally processed in appropriate order.
基金supported by the National Natural Science Foundation of China(Grant Nos.12102480,52278543 and 51978660)Natural Science Foundation of Jiangsu Province(Grant No.BK20231489)。
文摘Liquid-filled containers(LFC)are widely used to store and transport petroleum,chemical reagents,and other resources.As an important target of military strikes and terrorist bombings,LFC are vulnerable to blast waves and fragments.To explore the protective effect of polyurea elastomer on LFC,the damage characteristics of polyurea coated liquid-filled container(PLFC)under the combined loading of blast shock wave and fragments were studied experimentally.The microstructure of the polyurea layer was observed by scanning electron microscopy,and the fracture and self-healing phenomena were analyzed.The simulation approach was used to explain the combined blast-and fragments-induced on the PLFC in detail.Finally,the effects of shock wave and fragment alone and in combination on the damage of PLFC were comprehensively compared.Results showed that the polyurea reduces the perforation rate of the fragment to the LFC,and the self-healing phenomenon could also reduce the liquid loss rate inside the container.The polyurea reduces the degree of depression in the center of the LFC,resulting in a decrease in the distance between adjacent fragments penetrating the LFC,and an increase in the probability of transfixion and fracture between holes.Under the close-in blast,the detonation shock wave reached the LFC before the fragment.Polyurea does not all have an enhanced effect on the protection of LFC.The presence of internal water enhances the anti-blast performance of the container,and the hydrodynamic ram(HRAM)formed by the fragment impacting the water aggravated the plastic deformation of the container.The combined action has an enhancement effect on the deformation of the LFC.The depth of the container depression was 27%higher than that of the blast shock wave alone;thus,it cannot be simply summarized as linear superposition.
文摘Mongolia is a landlocked country with limited infrastructure and high dependence on the Xingang Tianjin port in China for imports. This research examines the potential impacts of establishing a dry port in Zamyn-Uud, Mongolia, utilizing a system dynamics modeling approach via Vensim software. The study evaluates transportation time, costs, inflation, and logistics performance index improvements, revealing that the establishment of the dry port can reduce transportation costs and delays significantly while enhancing economic growth. The findings offer actionable insights for policymakers and stakeholders in addressing logistical inefficiencies and fostering sustainable development in landlocked regions.
文摘Compacted sand-bentonite mixtures are used as waste containment barriers(e.g.landfill liners and bentonite buffers for nuclear waste)to restrict contaminant transport.The potential for enhanced chemical containment of compacted sand-bentonite mixtures due to semipermeable membrane behavior has also been demonstrated.However,the extent to which membrane behavior persists in the presence of highly concentrated chemical solutions,which have been shown to degrade membrane behavior in bentonite-based barriers,remains largely unknown.Moreover,the limiting(threshold)salt concentrations at which membrane behavior of compacted sand-bentonite mixtures is effectively destroyed have not been evaluated.Accordingly,this study quantified the limiting membrane behavior of two duplicated specimens of compacted sand-bentonite mixture comprising 15%sodium bentonite(by dry mass)by determining the limiting salt concentrations at which measurable membrane behavior was eliminated.The specimens were exposed to increasingly higher source concentrations,Cot,of boundary monovalent salt solutions(KCl and NaCl)until measured values of the membrane efficiency coefficient,ω,were effectively zero.Overall,ωdecreased from an average of 0.032 to zero as Cot increased from 160 mmol/L KCl to 3.27 mol/L NaCl,resulting in limiting threshold salt concentrations for the two tests between 1.63 mol/L and 3.27 mol/L NaCl,which are significantly higher than those at which measurable membrane behavior has previously been demonstrated.
文摘Virtualization is an indispensable part of the cloud for the objective of deploying different virtual servers over the same physical layer.However,the increase in the number of applications executing on the repositories results in increased overload due to the adoption of cloud services.Moreover,the migration of applications on the cloud with optimized resource allocation is a herculean task even though it is employed for minimizing the dilemma of allocating resources.In this paper,a Fire Hawk Optimization enabled Deep Learning Scheme(FHOEDLS)is proposed for minimizing the overload and optimizing the resource allocation on the hybrid cloud container architecture for migrating interoperability based applications This FHOEDLS achieves the load prediction through the utilization of deep CNN-GRU-AM model for attaining resource allocation and better migration of applications.It specifically adopted the Fire Hawk Optimization Algorithm(FHOA)for optimizing the parameters that influence the factors that aid in better interoperable application migration with improved resource allocation and minimized overhead.It considered the factors of resource capacity,transmission cost,demand,and predicted load into account during the formulation of the objective function utilized for resource allocation and application migration.The cloud simulation of this FHOEDLS is achieved using a container,Virtual Machine(VM),and Physical Machine(PM).The results of this proposed FHOEDLS confirmed a better resource capability of 0.418 and a minimized load of 0.0061.
基金funded by the European Commission H2020 Research and Innovation Programme through the HARNESSTOM innovation action(Grant No.101000716)Grant CIPROM/2021/020(project SOLECO)funded by Conselleria d’Innovació,Universitats,Ciència i Societat Digital(Generalitat Valenciana,Spain)Pietro Gramazio received a post-doctoral fellowship(Grant No.RYC2021-031999-I)funded by MCIN/AEI/10.13039/501100011033 and by“European Union NextGenerationEU/PRTR”。
文摘Unlike other major crops,little research has been performed on tomato to reduce the generation time for speed breeding.We evaluated several agronomic treatments to reduce the generation time of tomato in the‘M82'(determinate)and‘Moneymaker'(indeterminate)varieties and evaluated the best combination in conjunction with embryo rescue.Five container sizes with volumes of 0.2 L(XS),0.45 L(S),0.8 L(M),1.3 L(L),and6 L(XL),were evaluated in the first experiment under the autumn cycle.We found that plants grown in XL containers exhibited better development and required less time from sowing to anthesis(DSA)and from anthesis to fruit ripening(DAR).In the second experiment,using XL containers in the autumn-winter cycle,we evaluated the effects of cold priming at the cotyledonary stage,water stress,P supplementation,and K supplementation on generation time.Compared to the control,we found that cold priming significantly reduced the number of leaves,plant height to first the inflorescence,and DSA(2.7 d),whereas K supplementation reduced the DAR(8.8 d).In contrast,water stress and P supplementation did not significantly affect any of the measured traits like DAR,DSA or fruit set.To validate these data,in a third experiment with XL containers in the spring-summer cycle,the combination of cold priming and K supplementation was tested,confirming the significant effect of this combination on the reduction of generation time(2.9 d for DSA and 3.9 d for DAR)compared to the control.Embryo rescue during the cell expansion cycle(average of 22.0 d and 23.3 d after anthesis for‘M82'and‘Moneymaker',respectively)allowed the shortening of the generation time by 8.7 d in‘M82'and 11.6 d in‘Moneymaker'compared to the in planta fruit ripening.The combination of agronomic treatments with embryo rescue can effectively increase the number of generations per year from three to four for speed breeding of tomato.
