Bud dormancy is a complex physiological process of perennial woody plants living in temperate regions,and it can be affected by various phytohormones.Cytokinin oxidase/dehydrogenases(CKXs)are a group of enzymes essent...Bud dormancy is a complex physiological process of perennial woody plants living in temperate regions,and it can be affected by various phytohormones.Cytokinin oxidase/dehydrogenases(CKXs)are a group of enzymes essential for maintaining cytokinin homeostasis,yet a comprehensive analysis of these enzymes in peach remains lacking.Here,a total of 51 CKX members from different species,including six from peach,eleven from apple,nine from poplar,seven from Arabidopsis,eight from strawberry,and ten from rice,were identified using the Simple HMM Search tool of TBtools and a BLASTP program and classified into four groups using phylogenetic analysis.Conserved motif and gene structure analysis of these 51 CKX members showed that 10 conserved motifs were identified,and each CKX gene contained at least two introns.Cis-element analysis of PpCKXs showed that all PpCKX genes have light-responsive elements and at least one hormone-responsive element.The changed relative expression levels of six PpCKX genes in peach buds from endodormancy to bud-break were observed by qRT-PCR.Among them,the expression trend of PpCKX6 was almost opposite that of PpEBB1,a positive bud-break regulator in woody plants,around the bud-break stage.Y1H,EMSA,and dual-luciferase assays indicated that PpEBB1negatively regulated PpCKX6 through direct binding to a GCC box-like element located in the promoter region of PpCKX6.In addition,a transient assay showed that overexpression of PpCKX6 delayed the bud-break of peach.These results indicate that the PpCKX genes play an essential role in the dormancy-regrowth process,and Pp CKX6may act downstream of PpEBB1 directly to regulate the bud-break process,which further improves the hormoneregulatory network of dormancy-regrowth of woody plants,and provides new insights for molecular breeding and genetic engineering of peach.展开更多
Cytokinin oxidase/dehydrogenase(CKX; EC.1.5.99.12) regulates cytokinin(CK) level in plants and plays an essential role in CK regulatory processes. CKX proteins are encoded by a small gene family with a varying number ...Cytokinin oxidase/dehydrogenase(CKX; EC.1.5.99.12) regulates cytokinin(CK) level in plants and plays an essential role in CK regulatory processes. CKX proteins are encoded by a small gene family with a varying number of members in different plants. In spite of their physiological importance, systematic analyses of SiCKX genes in foxtail millet have not yet been examined. In this paper, we report the genome wide isolation and characterization of SiCKXs using bioinformatic methods. A total of 11 members of the family were identified in the foxtail millet genome. SiCKX genes were distributed in seven chromosomes(chromosome 1, 3, 4, 5, 6, 7, and 11). The coding sequences of all the SiCKX genes were disrupted by introns, with numbers varying from one to four. These genes expanded in the genome mainly due to segmental duplication events. Multiple alignment and motif display results showed that all SiCKX proteins share FAD- and CK-binding domains. Putative cis-elements involved in Ca2+-response, abiotic stress response, light and circadian rhythm regulation, disease resistance and seed development were present in the promoters of SiCKX genes. Expression data mining suggested that SiCKX genes have diverse expression patterns. Real-time PCR analysis indicated that all 11 SiCKX genes were up-regulated in embryos under 6-BA treatment, and some were NaCl or PEG inducible. Collectively, these results provide molecular insights into CKX research in plants.展开更多
Cytokinins are a class of phytohormones that promote cell division and differentiation and are thought to affect plant immunity to multiple pathogens.However,a comprehensive analysis of cytokinin dehydrogenase/oxidase...Cytokinins are a class of phytohormones that promote cell division and differentiation and are thought to affect plant immunity to multiple pathogens.However,a comprehensive analysis of cytokinin dehydrogenase/oxidase(CKX)family genes in cabbage has not been reported.In this study,a total of 36 CKX genes were identified using a genome-wide search method.Phylogenetic analysis classified these genes into three groups.They were distributed unevenly across nine chromosomes in B.oleracea,and 15 of them did not contain any introns.The results of colinearity analysis showed that 36 CKX gene in Arabidopsis was present in several copies in the Brassica oleracea genome.An analysis of cisacting elements indicated that all genes possessed at least one stress or hormone responsive cis-acting element.A heatmap of CKX gene expression showed the patterns of expression of these genes in various tissues and organs.Three genes(Bol028363,Bol031036 and Bol018140)were relatively highly expressed in all of the investigated tissues under normal conditions,showing the expression profile of housekeeping genes.Generally,the expression patterns of CKX genes in Jingfeng 1 and Xiangan 336 were quite different under the same treatment.Notably,three genes(Bol020547,Bol028392 and Bol045724)were significantly down-regulated and up-regulated in the susceptible and resistant material,respectively,after inoculation,which may indicate their crucial roles in resistance to clubroot disease.The results provide insights for better understanding the roles of CKX genes in the B.oleracea–P.brassicae interaction.展开更多
基金supported by the National Natural Science Foundation of China(32302509)the Weifang University Doctoral Research Launch Fund,China(2022BS18)the National Natural Science Foundation of China(32072518)。
文摘Bud dormancy is a complex physiological process of perennial woody plants living in temperate regions,and it can be affected by various phytohormones.Cytokinin oxidase/dehydrogenases(CKXs)are a group of enzymes essential for maintaining cytokinin homeostasis,yet a comprehensive analysis of these enzymes in peach remains lacking.Here,a total of 51 CKX members from different species,including six from peach,eleven from apple,nine from poplar,seven from Arabidopsis,eight from strawberry,and ten from rice,were identified using the Simple HMM Search tool of TBtools and a BLASTP program and classified into four groups using phylogenetic analysis.Conserved motif and gene structure analysis of these 51 CKX members showed that 10 conserved motifs were identified,and each CKX gene contained at least two introns.Cis-element analysis of PpCKXs showed that all PpCKX genes have light-responsive elements and at least one hormone-responsive element.The changed relative expression levels of six PpCKX genes in peach buds from endodormancy to bud-break were observed by qRT-PCR.Among them,the expression trend of PpCKX6 was almost opposite that of PpEBB1,a positive bud-break regulator in woody plants,around the bud-break stage.Y1H,EMSA,and dual-luciferase assays indicated that PpEBB1negatively regulated PpCKX6 through direct binding to a GCC box-like element located in the promoter region of PpCKX6.In addition,a transient assay showed that overexpression of PpCKX6 delayed the bud-break of peach.These results indicate that the PpCKX genes play an essential role in the dormancy-regrowth process,and Pp CKX6may act downstream of PpEBB1 directly to regulate the bud-break process,which further improves the hormoneregulatory network of dormancy-regrowth of woody plants,and provides new insights for molecular breeding and genetic engineering of peach.
基金supported by the project of the Modern Seed Industry Enterprise Science and Technology Development of Shandong Province, China (SDKJ2012QF003)
文摘Cytokinin oxidase/dehydrogenase(CKX; EC.1.5.99.12) regulates cytokinin(CK) level in plants and plays an essential role in CK regulatory processes. CKX proteins are encoded by a small gene family with a varying number of members in different plants. In spite of their physiological importance, systematic analyses of SiCKX genes in foxtail millet have not yet been examined. In this paper, we report the genome wide isolation and characterization of SiCKXs using bioinformatic methods. A total of 11 members of the family were identified in the foxtail millet genome. SiCKX genes were distributed in seven chromosomes(chromosome 1, 3, 4, 5, 6, 7, and 11). The coding sequences of all the SiCKX genes were disrupted by introns, with numbers varying from one to four. These genes expanded in the genome mainly due to segmental duplication events. Multiple alignment and motif display results showed that all SiCKX proteins share FAD- and CK-binding domains. Putative cis-elements involved in Ca2+-response, abiotic stress response, light and circadian rhythm regulation, disease resistance and seed development were present in the promoters of SiCKX genes. Expression data mining suggested that SiCKX genes have diverse expression patterns. Real-time PCR analysis indicated that all 11 SiCKX genes were up-regulated in embryos under 6-BA treatment, and some were NaCl or PEG inducible. Collectively, these results provide molecular insights into CKX research in plants.
基金supported by the Youth Science Fund Project(Grant No. 31801876)。
文摘Cytokinins are a class of phytohormones that promote cell division and differentiation and are thought to affect plant immunity to multiple pathogens.However,a comprehensive analysis of cytokinin dehydrogenase/oxidase(CKX)family genes in cabbage has not been reported.In this study,a total of 36 CKX genes were identified using a genome-wide search method.Phylogenetic analysis classified these genes into three groups.They were distributed unevenly across nine chromosomes in B.oleracea,and 15 of them did not contain any introns.The results of colinearity analysis showed that 36 CKX gene in Arabidopsis was present in several copies in the Brassica oleracea genome.An analysis of cisacting elements indicated that all genes possessed at least one stress or hormone responsive cis-acting element.A heatmap of CKX gene expression showed the patterns of expression of these genes in various tissues and organs.Three genes(Bol028363,Bol031036 and Bol018140)were relatively highly expressed in all of the investigated tissues under normal conditions,showing the expression profile of housekeeping genes.Generally,the expression patterns of CKX genes in Jingfeng 1 and Xiangan 336 were quite different under the same treatment.Notably,three genes(Bol020547,Bol028392 and Bol045724)were significantly down-regulated and up-regulated in the susceptible and resistant material,respectively,after inoculation,which may indicate their crucial roles in resistance to clubroot disease.The results provide insights for better understanding the roles of CKX genes in the B.oleracea–P.brassicae interaction.
