By inserting the CMV with HSV TK promoter into the plasmid pBlue-cGH which contained cGH gene, a chimeric expression vector was constructed for transgenic fish. After the vector was transferred into fertilized eggs of...By inserting the CMV with HSV TK promoter into the plasmid pBlue-cGH which contained cGH gene, a chimeric expression vector was constructed for transgenic fish. After the vector was transferred into fertilized eggs of crucian carp by means of microinjection, the transgenic crucian carp was obtained. The result of PCR detection showed that the growth hormone gene of the exogenous carp was integrated into the genome of crucian carp, and the transgenic crucian carp displayed enhancement of its growth.展开更多
Design method and procedures of computer-generated hologram （CGH） used for aspheric test are in- troduced in detail. For CGH phase calculation, virtual medium which has zero refractive index at given wavelength is u...Design method and procedures of computer-generated hologram （CGH） used for aspheric test are in- troduced in detail. For CGH phase calculation, virtual medium which has zero refractive index at given wavelength is used to model ideal aspheric wavefront. Reflective Fresnel zones located in a ring area concentric to the CGH structure is designed to reduce or eliminate alignment errors. Substrate figure error, pattern distortion, etching and duty cycle variations that influence the reconstructed wavefront are quantitatively analyzed in theory and corresponding error equations are obtained to guide the tolerance distribution during CGH fabricating. A design example is given and the uncertainty of t achieves λ20.展开更多
文摘By inserting the CMV with HSV TK promoter into the plasmid pBlue-cGH which contained cGH gene, a chimeric expression vector was constructed for transgenic fish. After the vector was transferred into fertilized eggs of crucian carp by means of microinjection, the transgenic crucian carp was obtained. The result of PCR detection showed that the growth hormone gene of the exogenous carp was integrated into the genome of crucian carp, and the transgenic crucian carp displayed enhancement of its growth.
基金the Ministerial Level Foundation (4645103030401)
文摘Design method and procedures of computer-generated hologram （CGH） used for aspheric test are in- troduced in detail. For CGH phase calculation, virtual medium which has zero refractive index at given wavelength is used to model ideal aspheric wavefront. Reflective Fresnel zones located in a ring area concentric to the CGH structure is designed to reduce or eliminate alignment errors. Substrate figure error, pattern distortion, etching and duty cycle variations that influence the reconstructed wavefront are quantitatively analyzed in theory and corresponding error equations are obtained to guide the tolerance distribution during CGH fabricating. A design example is given and the uncertainty of t achieves λ20.
