HighlightsA novel conjugative plasmid pHJ90-cfr carrying the multiresistance gene cfr was characterized in Proteus vulgaris.A new IS5-family member,ISPmi4,was identified for the first time.Both plasmids and ICEs were ...HighlightsA novel conjugative plasmid pHJ90-cfr carrying the multiresistance gene cfr was characterized in Proteus vulgaris.A new IS5-family member,ISPmi4,was identified for the first time.Both plasmids and ICEs were vital mobile genetic elements for horizontal transmission of cfr gene in Proteus species.展开更多
This paper introduces a groundbreaking synthesis of fundamental quantum mechanics with the Advanced Observer Model (AOM), presenting a unified framework that reimagines the construction of reality. AOM highlights the ...This paper introduces a groundbreaking synthesis of fundamental quantum mechanics with the Advanced Observer Model (AOM), presenting a unified framework that reimagines the construction of reality. AOM highlights the pivotal role of the observer in shaping reality, where classical notions of time, space, and energy are reexamined through the quantum lens. By engaging with key quantum equations—such as the Schrödinger equation, Heisenberg uncertainty principle, and Dirac equation—the paper demonstrates how AOM unifies the probabilistic nature of quantum mechanics with the determinism of classical physics. Central to this exploration is the Sequence of Quantum States (SQS) and Constant Frame Rate (CFR), which align with concepts like quantum superposition, entanglement, and wave function collapse. The model’s implications extend to how observers perceive reality, proposing that interference patterns between wave functions form the foundation of observable phenomena. By offering a fresh perspective on the interplay between determinacy and indeterminacy, AOM lays a robust theoretical foundation for future inquiry into quantum physics and the philosophy of consciousness.展开更多
This paper introduces the Advanced Observer Model (AOM), a novel framework that integrates classical mechanics, quantum mechanics, and relativity through the observer’s role in constructing reality. Central to the AO...This paper introduces the Advanced Observer Model (AOM), a novel framework that integrates classical mechanics, quantum mechanics, and relativity through the observer’s role in constructing reality. Central to the AOM is the Static Configuration/Dynamic Configuration (SC/DC) conjugate, which examines physical systems through the interaction between static spatial configurations and dynamic quantum states. The model introduces a Constant Frame Rate (CFR) to quantize time perception, providing a discrete model for time evolution in quantum systems. By modifying the Schrödinger equation with CFR, the AOM bridges quantum and classical physics, offering a unified interpretation where classical determinism and quantum uncertainty coexist. A key feature of the AOM is its energy scaling model, where energy grows exponentially with spatial dimensionality, following the relationshipE∝(π)n. This dimensional scaling connects the discrete time perception of the observer with both quantum and classical energy distributions, providing insights into the nature of higher-dimensional spaces. Additionally, the AOM posits that spacetime curvature arises from quantum interactions, shaped by the observer’s discrete time perception. The model emphasizes the observer’s consciousness as a co-creator of reality, offering new approaches to understanding the quantum-classical transition. While speculative, the AOM opens new avenues for addressing foundational questions in quantum mechanics, relativity, dimensionality, and the nature of reality.展开更多
目的探讨利奈唑胺非敏感粪肠球菌相关耐药机制及同源性。方法收集2014年5月至2015年9月临床分离利奈唑胺非敏感粪肠球菌11株,采用E-test复核菌株对利奈唑胺、万古霉素、替考拉宁的最低抑菌浓度（MIC）;PCR扩增和测序检测cfr基因、23S r ...目的探讨利奈唑胺非敏感粪肠球菌相关耐药机制及同源性。方法收集2014年5月至2015年9月临床分离利奈唑胺非敏感粪肠球菌11株,采用E-test复核菌株对利奈唑胺、万古霉素、替考拉宁的最低抑菌浓度（MIC）;PCR扩增和测序检测cfr基因、23S r RNA第5功能区以及L3、L4核糖体位点基因;脉冲场凝胶电泳（PFGE）分析菌株同源性。结果 11株粪肠球菌对利奈唑胺MIC范围为4～48 mg/L,其中耐药7株,中介4株,对万古霉素和替考拉宁均敏感;均未检出cfr基因,在23S r RNA第5功能区及核糖体位点均未检出突变;PFGE显示各菌株电泳条带差异较大,以A克隆最多。结论造成我院粪肠球菌对利奈唑胺敏感性降低机制不明,不存在克隆株的播散。展开更多
基金supported by the National Key Research and Development Program of China(2022YFF0710505)the Central Public-interest Scientific Institution Basal Research Fund,China(1610302022001)。
文摘HighlightsA novel conjugative plasmid pHJ90-cfr carrying the multiresistance gene cfr was characterized in Proteus vulgaris.A new IS5-family member,ISPmi4,was identified for the first time.Both plasmids and ICEs were vital mobile genetic elements for horizontal transmission of cfr gene in Proteus species.
文摘This paper introduces a groundbreaking synthesis of fundamental quantum mechanics with the Advanced Observer Model (AOM), presenting a unified framework that reimagines the construction of reality. AOM highlights the pivotal role of the observer in shaping reality, where classical notions of time, space, and energy are reexamined through the quantum lens. By engaging with key quantum equations—such as the Schrödinger equation, Heisenberg uncertainty principle, and Dirac equation—the paper demonstrates how AOM unifies the probabilistic nature of quantum mechanics with the determinism of classical physics. Central to this exploration is the Sequence of Quantum States (SQS) and Constant Frame Rate (CFR), which align with concepts like quantum superposition, entanglement, and wave function collapse. The model’s implications extend to how observers perceive reality, proposing that interference patterns between wave functions form the foundation of observable phenomena. By offering a fresh perspective on the interplay between determinacy and indeterminacy, AOM lays a robust theoretical foundation for future inquiry into quantum physics and the philosophy of consciousness.
文摘This paper introduces the Advanced Observer Model (AOM), a novel framework that integrates classical mechanics, quantum mechanics, and relativity through the observer’s role in constructing reality. Central to the AOM is the Static Configuration/Dynamic Configuration (SC/DC) conjugate, which examines physical systems through the interaction between static spatial configurations and dynamic quantum states. The model introduces a Constant Frame Rate (CFR) to quantize time perception, providing a discrete model for time evolution in quantum systems. By modifying the Schrödinger equation with CFR, the AOM bridges quantum and classical physics, offering a unified interpretation where classical determinism and quantum uncertainty coexist. A key feature of the AOM is its energy scaling model, where energy grows exponentially with spatial dimensionality, following the relationshipE∝(π)n. This dimensional scaling connects the discrete time perception of the observer with both quantum and classical energy distributions, providing insights into the nature of higher-dimensional spaces. Additionally, the AOM posits that spacetime curvature arises from quantum interactions, shaped by the observer’s discrete time perception. The model emphasizes the observer’s consciousness as a co-creator of reality, offering new approaches to understanding the quantum-classical transition. While speculative, the AOM opens new avenues for addressing foundational questions in quantum mechanics, relativity, dimensionality, and the nature of reality.
文摘目的探讨利奈唑胺非敏感粪肠球菌相关耐药机制及同源性。方法收集2014年5月至2015年9月临床分离利奈唑胺非敏感粪肠球菌11株,采用E-test复核菌株对利奈唑胺、万古霉素、替考拉宁的最低抑菌浓度（MIC）;PCR扩增和测序检测cfr基因、23S r RNA第5功能区以及L3、L4核糖体位点基因;脉冲场凝胶电泳（PFGE）分析菌株同源性。结果 11株粪肠球菌对利奈唑胺MIC范围为4～48 mg/L,其中耐药7株,中介4株,对万古霉素和替考拉宁均敏感;均未检出cfr基因,在23S r RNA第5功能区及核糖体位点均未检出突变;PFGE显示各菌株电泳条带差异较大,以A克隆最多。结论造成我院粪肠球菌对利奈唑胺敏感性降低机制不明,不存在克隆株的播散。
