Background Broiler chickens are most vulnerable immediately after hatching due to their immature immune systems,making them susceptible to infectious diseases.The yolk plays an important role in early immune defence b...Background Broiler chickens are most vulnerable immediately after hatching due to their immature immune systems,making them susceptible to infectious diseases.The yolk plays an important role in early immune defence by showing relevant antioxidant and passive immunity capabilities during broiler embryonic development.The immunomodulatory effects of phytogenic compound carvacrol have been widely reported.After in ovo delivery in the amniotic fluid during embryonic development carvacrol is known to migrate to the yolk sac.However,it is unknown whether carvacrol in the yolk could enhance defence responsiveness in the yolk sac.Therefore,the aim of this study was to improve early immune function in chicken embryos,and it was hypothesized that in ovo delivery of carvacrol would result in immunomodulatory effects in the yolk sac,potentially improving post-hatch resilience.Methods On embryonic day(E)17.5,either a saline(control)or carvacrol solution was injected into the amniotic fluid.Yolk sac tissue samples were collected at E19.5,and transcriptomic analyses using RNA sequencing were performed,following functional enrichment analyses comparing the control(saline)and carvacrol-injected groups.Results The results showed that 268 genes were upregulated and 174 downregulated in the carvacrol group compared to the control(P<0.05;logFC<-0.5 or log FC>0.5).Functional analyses of these differentially expressed genes,using KEGG,REACTOME,and Gene Ontology databases,showed enrichment of several immune-related pathways.This included the pathways‘Antimicrobial peptides’(P=0.001)and‘Chemoattractant activity’(P=0.004),amongst others.Moreover,the‘NOD-like receptor signaling’pathway was enriched(P=0.002).Antimicrobial peptides are part of the innate immune defence and are amongst the molecules produced after the nucleotide oligomeriza-tion domain(NOD)-like receptor pathway activation.While these responses may be associated with an inflammatory reaction to an exogenous threat,they could also indicate that in ovo delivery of carvacrol could prepare the newly hatched chick against bacterial pathogens by potentially promoting antimicrobial peptide production through acti-vation of NOD-like receptor signaling in the yolk sac.Conclusion In conclusion,these findings suggest that in ovo delivery of carvacrol has the potential to enhance anti-pathogenic and pro-inflammatory responses in the yolk sac via upregulation of antimicrobial peptides,and NOD-like receptor pathways.展开更多
Practical application of carvacrol in different fields including foods and biopesticides has been limited due to its instability and water insolubility.In this work,carvacrol encapsulated Pickering emulsion is develop...Practical application of carvacrol in different fields including foods and biopesticides has been limited due to its instability and water insolubility.In this work,carvacrol encapsulated Pickering emulsion is developed by using polymeric Janus nanoparticles as the stabilizer.To achieve this,dumbbell-shaped polymeric nanoparticles composed of two spheres of shellac and polylactic acid(PLA)are firstly prepared via co-precipitation in a rotating packed bed reactor,followed by grafting of chitooligosaccharides(COS)onto shellac to synthesis amphiphilic Janus nanoparticles(PLA/shellac-COS).Pickering emulsions with typical oil-in-water,bi-continuous structure and water-in-oil characteristics are produced by configuring carvacrol emulsions with different oil-to-water ratios.The stability of emulsions with 5%carvacrol content stabilized by 0.5% PLA/shellac-COS nanoparticles were more stable when compared to those prepared by shellac nanoparticles and PLA/shellac nanoparticles.After stored for one month,the carvacrol encapsulated Pickering emulsions maintained a high zeta potential of-43.8 mV,with no significant changes in particle size.These preliminary studies illustrated that polymeric Janus nanoparticles synthesized by co-precipitation in a rotating packed bed are promising particles for Pickering emulsions and related work in the future.展开更多
Background： In the post-antibiotic era, essential oils （EO） are promising alternatives to growth-promoting antibiotics. The aim of the present study was to investigate the antibacterial activities of an EO product ...Background： In the post-antibiotic era, essential oils （EO） are promising alternatives to growth-promoting antibiotics. The aim of the present study was to investigate the antibacterial activities of an EO product and its components thymol and carvacrol in vitro, and the efficacy of EO to control Clostridium perffingens challenge in broiler chickens. Results： The in vitro minimum inhibitory concentration assay showed strong antibacterial activity of the EO product, thymol, and carvacrol against pathogenic Escherichia col~, C. perffingens, and Salmonella strains, and weak activity towards beneficial Lactobacillus strains. Besides, an additive effect was observed between thymol and carvacrol. The in vivo study was carried out with 448 male broiler chicks following a 4 x 2 factorial arrangement to test the effects of EO supplementation （0, 60, 120, or 240 mg/kg EO in wheat-based diet）, pathogen challenge （with or without oral gavage of C. perfr/ngens from day 14 to day 20） and their interactions. Each treatment consisted of eight replicate pens （seven birds/pen）. The challenge led to macroscopic gut lesions, and resulted in a significant increase in ileal populations of C. perffingens and Eschefichia subgroup （P ≤ 0.05） on day 21. Dietary EO supplementation did not influence C. perfringens numbers, but linearly alleviated intestinal lesions on day 21 and 28 （P= 0.010 and 0.036, respectively）, and decreased Eschefichia populations in ileum with increased EO dosages （P = 0.027 and 0.071 for day 21 and 28, respectively）. For caecum, EO quadratically influenced Lactobacillus populations on day 21 （P = 0.002）, and linearly decreased the numbers of total bacteria and Eschefichio on day 28 （P = 0.026 and 0.060, respectively）. Mean thymol and carvacrol concentrations in the small intestine were 0.21 and 0.20 μg/g in intestinal digesta （wet weight）, respectively, for birds fed 60 mg/kg EO, and 0.80 and 0.71 μg/g, respectively, for birds fed 240 mg/kg EO. Conclusions： These results indicated that dietary EO supplementation could affect intestinal microbiota and alleviate intestinal lesions in broilers, which may contribute in controlling C. perffingens infection in broiler chickens.展开更多
Background:Over the past years,antibiotic growth promoter had been restricted in animal husbandry production in many countries because of antimicrobial resistance and foodborne antibiotic residues.However,the problems...Background:Over the past years,antibiotic growth promoter had been restricted in animal husbandry production in many countries because of antimicrobial resistance and foodborne antibiotic residues.However,the problems of poor intestinal health and low growth efficiency of piglets have not been solved completely in an antibiotic-free diet,and it is urgent to explore alternatives to antimicrobial growth promoters.Methods:Here,a total of 532 weaned pigs were assigned to one of 4 treatments,the low amino acid(AA)level diet(d 1 to d 14 is 1.35%,d 15 to d 42 is 1.25%)(Low AA),the low AA level diet supplementation with a carvacrol-thymol blend(50 mg carvacrol and 50 mg thymol/kg of diet)(CB)(Low AA+CB),the high AA level diet(d 1 to d 14 is 1.50%,d 15 to d 42 is 1.40%)(High AA),and the high AA level diet supplementation with a CB(High AA+CB),respectively.Then we measured growth performance and intestinal health indicators of weaned pigs.Results:Results showed that high AA level significantly reduced plasma urea nitrogen,plasma Interleukin-6(IL-6)and fecal lipocalin-2 contents(P<0.05),significantly increased the relative abundance of fecal Lactobacillus and Enterococcus,and had a trend to increase the fecal secretory immunoglobulin A(sIgA)and mucin 2(MUC 2)contents(P<0.05)in piglets,thereby alleviating the diarrhea of piglets and reducing the feed conversion ratio(FCR)of piglets during d 1~14 after weaning.Dietary supplementation with CB significantly increased the activity of plasma antioxidant enzymes T-SOD and GSH-px(P<0.05),while significantly reduced plasma malondialdehyde(MDA),plasma interleukin-1β(IL-1β),plasma endotoxin and D-lactic acid contents(P<0.05).Meanwhile,CB significantly decreased fecal lipocalin-2 contents and the abundance of fecal Escherichia coli(P<0.05).Thus,we hypothesis that dietary supplementation with CB significantly increased the average daily gain(ADG)of piglets(P<0.05)during d 1~14 after weaning through promoting intestinal health.Conclusion:These results suggest that high AA level and dietary supplementation with CB improved the growth performance of weaned pigs in an antibiotic-free diet by improving AA metabolism and intestinal antioxidant capacity.展开更多
Objective:To unravel the mechanism of anti-inflammatory activity of carvacrol in D-galactosamine(D-GalN)-induced hepatotoxic rats.Methods:The mRNA and protein expression levels of tumor necrosis factor-alpha(TNF-α).i...Objective:To unravel the mechanism of anti-inflammatory activity of carvacrol in D-galactosamine(D-GalN)-induced hepatotoxic rats.Methods:The mRNA and protein expression levels of tumor necrosis factor-alpha(TNF-α).interleukin-6[IL-6).inducible nitric oxide synthase(iNOS),cyelooxy genase-2(COX-2) and nuclear faclor kappa-B(NF-κB) were assayed by semi-quantitative reverse transcriptase polymerase chain reaction(RTPCR) and western blot analysis.Results:We found that the mRNA and protein expressions of TNF-α. IL-6,iNOS,COX-2 and NF-κB were significanlly up-regulated in D-galactosamine induced hepatotoxic rats and treatment with carvacrol significantly down-regulated the expressions of these genes showing the mechanism behind the anti-inflammatory activity of carvacrol. Conclusions:All above results reveal that the carvacrol well known anti-inflammatory activities in D-galactosamine induced hepatotoxic rats.展开更多
Medicinal plants are rich in nutrients and phytochemicals which prevent and treat a wide range of ailments.Accumulating experimental studies exhibit that some bioactive ingredients extracted from medicinal plants have...Medicinal plants are rich in nutrients and phytochemicals which prevent and treat a wide range of ailments.Accumulating experimental studies exhibit that some bioactive ingredients extracted from medicinal plants have suitable therapeutic effects on hepatic and renal injuries.This review focuses on the hepato-and reno-protective effects of thymoquinone,crocin,and carvacrol.The relevant literature was retrieved from PubMed,Scopus,Web of Science,and Google Scholar databases from the beginning of 2015 until the end of November 2021.According to the scientific evidence,the considered phytochemicals in this review have been applied with useful therapeutic effects on hepatic and renal damage.These therapeutic effects were mainly mediated through the amelioration of oxidative stress,suppression of inflammatory responses,and inhibition of apoptosis.Intracellular signaling pathways linked to nuclear factor kappa B(NF-κB),adenosine monophosphate-activated protein kinase,c-jun N-terminal kinase,and extracellular signal-regulated kinase 1/2 and Toll-like receptors are the most important pathways targeted by these phytochemicals.Up-regulation of transcription factor Nrf2 and down-regulation of transforming growth factor-beta 1 by these natural compounds also contribute to the alleviation of hepatic and renal injuries.展开更多
Nine new sulfonamides derived from carvacrol were prepared through a reaction between 4-hydroxy-2-isopropyl-5-methyl benzene sulfonyl chloride with various amines in excellent yields (76% - 92%). The sulfonamides were...Nine new sulfonamides derived from carvacrol were prepared through a reaction between 4-hydroxy-2-isopropyl-5-methyl benzene sulfonyl chloride with various amines in excellent yields (76% - 92%). The sulfonamides were characterized using spectrometric and spectroscopic methods. Among these compounds, three derivatives showed excellent results in antibacterial activity against resistant S. aureus strains, with MIC values ranging from 3.9 to 62.50 ppm. The sulfonamide derivative of 4-methylaniline (SULF-1) had the best performance for all tested strains of bacteria (MIC = 3.9 to 15.62 ppm). Furthermore, the sulfonamide derivative of 4-fluoro aniline (SULF-3), which also presented promising results, was found to have a synergistic effect when combined with tetracycline and partial synergistic effect when combined with ampicillin, exhibiting an FIC index between 0.50 and 0.75. The sulfonamide derivative of 4-methylaniline had a synergistic effect in combination with erythromycin exhibiting an FIC index of 0.37. Carvacrol in association with the antibiotics tested did not have a synergistic effect.展开更多
The aim of this study was to elucidate the mode of action of two antimicrobial compounds, nisin, which is hydrophilic, and the lipophilic compound carvacrol. For that purpose, both antimicrobials were loaded into Oil-...The aim of this study was to elucidate the mode of action of two antimicrobial compounds, nisin, which is hydrophilic, and the lipophilic compound carvacrol. For that purpose, both antimicrobials were loaded into Oil-in-/Water nanoemulsions, and their antimicrobial activity against Bacillus subtilis was investigated. The experimental results indicated that the O/W nanoemulsions loaded with both, nisin and carvacrol, having Sauter mean diameter （d3,2） of around 120 nm, were considerably stable under the conditions investigated （up to 1 week storage at room temperature）. The interfacial tension between nisin aqueous solutions and soybean oil could be reduced up to 12 mN/m, as compared to that of pure water and soybean oil （21 mN/m）, so that demonstrating the potential of nisin to be used as emulsifier. The results obtained for O/W nanoemulsions loaded with both antimicrobial compounds indicated a reduction of nearly two log cycles （around 100 times） on B. subtilis population upon incubation for 24 h, compared to the blank （10 mM phosphate buffer）.展开更多
The product of plant essential oil, oregano carvacrol, was added in the diet of broiler, to study the effect of oregano carvacrol on growth performance of broilers. Using single factor test, 190 663 individuals of hea...The product of plant essential oil, oregano carvacrol, was added in the diet of broiler, to study the effect of oregano carvacrol on growth performance of broilers. Using single factor test, 190 663 individuals of healthy AA broilers at 0 day old were randomly divided into two groups, eight repeats in control group and four repeats in experimental group. The broilers in control group were fed with basal diet （containing 10 mg/kg cnramycin and 60 mg/kg salinomycin sodium）, and those in experimental groups were fed with the basal diet adding with 100 mg/kg oregano carvacrol. Compared with control group, the daily gain of broilers in exper- imental group was increased noticeably by 3.41 g/broiler （P 〈 0. 05） ; the slaughter quality was increased remarkably by 143 g/broiler （P 〈 0.05 ） ; the survival rate was increased significantly by 3.01% （ P 〈 0.05 ） ; and the feed gain ratio was reduced markedly by 0.08 （ P 〈 0.05 ）. The paper provided a theoretical basis for application of oregano carvacrol in broiler production.展开更多
Background:Zataria multiflora and carvacrol showed various pharmacological prop-erties including anti-inflammatory and anti-oxidant effects.However,up to now no studies have explored its potential benefits in ameliora...Background:Zataria multiflora and carvacrol showed various pharmacological prop-erties including anti-inflammatory and anti-oxidant effects.However,up to now no studies have explored its potential benefits in ameliorating sepsis-induced aortic and cardiac injury.Thus,this study aimed to investigate the effects of Z.multiflora and carvacrol on nitric oxide(NO)and oxidative stress indicators in lipopolysaccharide(LPS)-induced aortic and cardiac injury.Methods:Adult male Wistar rats were assigned to:Control,lipopolysaccharide(LPS)(1 mg/kg,intraperitoneal(i.p.)),and Z.multiflora hydro-ethanolic extract(ZME,50–200 mg/kg,oral)-and carvacrol(25–100 mg/kg,oral)-treated groups.LPS was in-jected daily for 14 days.Treatment with ZME and carvacrol started 3 days before LPS administration and treatment continued during LPS administration.At the end of the study,the levels of malondialdehyde(MDA),NO,thiols,and antioxidant enzymes were evaluated.Results:Our findings showed a significant reduction in the levels of superoxide dis-mutase(SOD),catalase(CAT),and thiols in the LPS group,which were restored by ZME and carvacrol.Furthermore,ZME and carvacrol decreased MDA and NO in car-diac and aortic tissues of LPS-injected rats.Conclusions:The results suggest protective effects of ZME and carvacrol on LPS-induced cardiovascular injury via improved redox hemostasis and attenuated NO pro-duction.However,additional studies are needed to elucidate the effects of ZME and its constituents on inflammatory responses mediated by LPS.展开更多
The present study investigated the effects of phytoadditive carvacrol on growth performance, feed utilization, hematological, non-specific immune and serum biochemical parameters in rainbow trout (Oncorhynchus mykiss)...The present study investigated the effects of phytoadditive carvacrol on growth performance, feed utilization, hematological, non-specific immune and serum biochemical parameters in rainbow trout (Oncorhynchus mykiss). In trial, 240 rainbow trout which had average weight of 10.79 ± 0.57 was used. Fish were divided into four groups before being fed for 60 days with 0, 1 (C1), 3 (C3) or 5 (C5) g/kg of carvacrol. There were no particular differences in the growth performance, feed utilization, whole body composition, hematological parameters between the experimental group and the control group. However, as compared to control group, a significant increase of serum lysozyme activity was seen in groups receiving feed containing C3 and C5 groups after 30 days. The myeloperoxidase activity in groups (C1 and C3) was significantly higher on the 30th day of sampling. Myeloperoxidase activity of C5 treated group was significantly higher on the 60th day in comparison with the control group. Serum glucose levels significantly decreased on 60th day for C1, C3 and C5 treated groups. The serum total protein, globulin and triglyceride levels of C3 treated groups was significantly higher on the 60th day in comparison with the control and other carvacrol treated groups. The level of serum cholesterol in C5 treated groups was significantly lower on the 30th day in comparison with the control and other carvacrol treated groups. The results of the present study indicated that dietary supplementation of 3 g/kg of carvacrol in commercial diets could improve some non-specific and biochemical status in rainbow trout.展开更多
The virulence factors including biofilm and virulence proteins in Listeria monocytogenes(L.monocytogenes)threatens human health.Herein,the inhibitory mechanisms of carvacrol(CAR)on the biofilm formation and virulence ...The virulence factors including biofilm and virulence proteins in Listeria monocytogenes(L.monocytogenes)threatens human health.Herein,the inhibitory mechanisms of carvacrol(CAR)on the biofilm formation and virulence production in L.monocytogenes were explored.It was found the minimum biofilm inhibition concentration of CAR was 0.8 mg/mL.CAR could effectively inhibit bacterial biofilm formation by reduction of extracellular polymeric substance,including extracellular polysaccharides,proteins,and DNA.CAR could interfere with the bacterial swarming and swimming motilities,thereby hindering biofilm development.Hemolytic capability of Listeriolysin O was significantly decreased from 85.75%to 28.13%after treatment with CAR at the minimum inhibitory concentration(MIC).Transcriptomic analysis revealed exposure to 1/2 MIC of CAR resulted in 969 differentially expressed genes,with downregulation of virulence factor-related genes.CAR might bind to AgrB within the quorum sensing system,thereby affecting the biofilm formation regulation and virulence expression.In addition,CAR exhibited efficient inhibitory effects on the biofilm formation on different food contacting materials and vegetable surfaces.These findings provided new insights into biofilm-inhibition and virulence-reduction mechanisms of CAR on L.monocytogenes,suggesting potential applications in controlling L.monocytogenes biofilm contamination in food industries.展开更多
The use of natural products as antibiotic adjuvants to enhance efficacy and mitigate resistance is increasingly recog-nized as a promising strategy.This study explored five novel synergistic antimicrobial combinations...The use of natural products as antibiotic adjuvants to enhance efficacy and mitigate resistance is increasingly recog-nized as a promising strategy.This study explored five novel synergistic antimicrobial combinations(SACs)of carvac-rol(CARV)and three already identified SACs of thymol(THY)with chloramphenicol,gentamicin,and streptomycin against Staphylococcus aureus and Acinetobacter baumannii,critical WHO-listed pathogens,and investigated their mechanisms of action and resistance-prevention capabilities.Despite being isomers,CARV and THY exhibited distinct synergistic effects and fractional inhibitory concentration index(FICI)values depending on the antibiotic and bac-terial species.The SACs significantly reduced the required antibiotic dose by 4-to 16-fold,with FICI values ranging from 0.25 to 0.5.Growth kinetics revealed that SACs completely inhibited planktonic bacterial growth,outperform-ing antibiotics alone.Additionally,the SACs demonstrated efficacy in both inhibiting and eradicating biofilms of S.aureus and A.baumannii.Resistance development studies highlighted that neither THY nor CARV induced resistance in these pathogens.Moreover,SACs combining aminoglycosides with THY reduced the emergence of resistance in A.baumannii by up to 32-fold.In S.aureus,THY mitigated gentamicin resistance by 16-fold.CARV exhibited similar,albeit slightly less potent,effects.Mechanistic investigations revealed that THY and CARV exert antimicrobial action by multiple mechanisms,includ-ing bacterial membrane depolarization and disruption,efflux pump inhibition,disrupting ATP metabolism and miti-gating oxidative stress induced by antibiotics.These findings highlight the potential of SACs to enhance antibiotic efficacy while preventing resistance,positioning them as strong candidates for innovative antimicrobial therapies against multidrug-resistant pathogens.展开更多
In this study,carvacrol nanoemulsion(CN)was prepared using ultrasonic emulsification and the synergistic bactericidal effect of ultrasound(US)and CN on Listeria monocytogenes and its mechanism were investigated.The re...In this study,carvacrol nanoemulsion(CN)was prepared using ultrasonic emulsification and the synergistic bactericidal effect of ultrasound(US)and CN on Listeria monocytogenes and its mechanism were investigated.The results showed that the combined treatment of US and CN had a significant(P<0.05)synergistic effect on the inactivation of L.monocytogenes,with synergistic values(SV)of 1.345 and 1.325,respectively.US+CN disrupts mononucleosis cell membranes and increases their permeability,as demonstrated by confocal laser scanning microscopy,flow cytometry and cell content release assays.N-phenyl-1-naphthylamine,reactive oxygen species and malondialdehyde assays showed that the combined treatment accelerated cellular oxidative stress and peroxidation,thereby affecting membrane lipid homeostasis.Field emission scanning electron microscopy showed that US+CN caused cell membranes to dry out and crumble.In addition,US+CN effectively kill L.monocytogenes on the surface of cabbages,reduce the weight loss of cabbages during storage,and had no significant(P>0.05)effect on its color and hardness.This study demonstrated that US+CN has the potential to inactivate foodborne pathogens and improve the safety of fresh produce,providing theoretical possibilities for its application.展开更多
The contamination of L.monocytogenes on different foods mainly achieves by the formation of biofilm,and L.monocytogenes biofilm poses great risks to food industry.Hence,exploring the safe and nontoxic substances to in...The contamination of L.monocytogenes on different foods mainly achieves by the formation of biofilm,and L.monocytogenes biofilm poses great risks to food industry.Hence,exploring the safe and nontoxic substances to interfere and destroy L.monocytogenes biofilm is of great concern.In this research,carvacrol was selected as the antibiofilm substance to investigate its effect on the three-dimensional structure,extracellular polymer matrix,aggregation,adhesion and Agr quorum sensing system of L.monocytogenes biofilm.The results revealed that L.monocytogenes showed the greatest biofilm formation ability in 5 days cultivation on stainless steel sheet.The minimum biofilm inhibition concentration and minimum biofilm elimination concentration of carvacrol against L.monocytogenes biofilm were 0.4 and 0.8 mg/mL.SEM and fluorescent pictures demonstrated that carvacrol could destroy and eliminate polymer matrix of L.monocytogenes biofilm.Furthermore,carvacrol could impact the swimming motility and aggregation ability of L.monocytogenes to inhibit the adhesion and initial formation of biofilm.Finally,carvacrol also could effectively inhibit the secretion and production of exopolysaccharide and proteins,downregulating the gene expression and binding with signaling proteins in Agr quorum sensing system to interfere the formation of biofilm.In conclusion,carvacrol could be applied as the ideal antibiofilm substance to inhibit and destroy L.monocytogenes biofilm in food industry.展开更多
Objective:To entrap carvacrol(CAR)in bovine serum albumin nanoparticles(BSANPs)to form CAR-loaded BSANPs(CAR@BSANPs)and to explore the anti-cancer effects in breast adenocarcinoma cells(MCF-7 cells)treated with CAR an...Objective:To entrap carvacrol(CAR)in bovine serum albumin nanoparticles(BSANPs)to form CAR-loaded BSANPs(CAR@BSANPs)and to explore the anti-cancer effects in breast adenocarcinoma cells(MCF-7 cells)treated with CAR and CAR@BSANPs.Methods:A desolvation method was used to synthesize BSANPs and CAR@BSANPs.The BSANPs and CAR@BSANPs were characterized by several physicochemical methods,including visual observation,high-resolution field emission scanning electron microscopy,Fourier transform infrared spectroscopy,and high-performance liquid chromatography.MCF-7 cells were used and analyzed after 24 h of exposure to CAR and CAR@BSANPs at half-maximal inhibitory concentration.The anti-proliferative,apoptotic,reactive oxygen species(ROS),and nitric oxide(NO)scavenging activity as well as gene expression analysis were investigated by the cell viability assay,phase-contrast microscopy,2',7'-dichlorofluorescein-diacetate assay,Griess-Illosvoy colorimetric assay,and quantitative real-time polymerase chain reaction,respectively.Results:CAR and CAR@BSANPs showed anti-proliferative,apoptotic,ROS generation,and NO scavenging effects on MCF-7 cells.Expression profile of B-cell lymphoma 2-like 11(BCL2L11),vascular endothelial growth factor A(VEGFA),hypoxia inducible factor factor-1α(HIF1A),BCL2L11/apoptosis regulator(BAX),and BCL2L11/Bcl2 homologous antagonist/killer 1(BAK1)ratios revealed downregulated genes;and BAX,BAK1,and CASP8 were upregulated by CAR and CAR@BSANPs treatment.In vitro anticancer assays of the CAR and CAR@BSANPs showed that CAR@BSANPs demonstrated higher therapeutic efficacy in the MCF-7 cells than CAR.Conclusions:CAR and CAR@BSANPs affect gene expression and may subsequently reduce the growth and proliferation of the MCF-7 cells.Molecular targeting of regulatory genes of the MCF-7 cells with CAR and CAR@BSANPs may be an effective therapeutic strategy against breast cancer.展开更多
Poor prognosis is associated with oral squamous cell carcinoma(OSCC),an aggressive form of malignant tumor.1 This study aimed to investigate the pharmacological effects of carvacrol on OSCC by targeting the tumor-asso...Poor prognosis is associated with oral squamous cell carcinoma(OSCC),an aggressive form of malignant tumor.1 This study aimed to investigate the pharmacological effects of carvacrol on OSCC by targeting the tumor-associated antigen FGFR1.As a key survival biomarker in OSCC,FGFR1 plays a crucial role in malignant transformation.Carvacrol,a specific FGFR1 inhibitor,induces its degradation via the ubiquitin-proteasome pathway.展开更多
Objective:To examine the role of carvacrol in modulating PI3 K/AKT signaling involved in human breast cancer pathogenesis using in vitro experimental model MCF-7 cells.Methods:MTT and lactate dehydrogenase assays were...Objective:To examine the role of carvacrol in modulating PI3 K/AKT signaling involved in human breast cancer pathogenesis using in vitro experimental model MCF-7 cells.Methods:MTT and lactate dehydrogenase assays were performed with cells treated with different doses of carvacrol(0–250μmol/L)at different time points(24 and 48 h).The nuclear morphology was assessed in MCF-7 cells with propidium iodide(PI)and acridine orange/ethidium bromide(AO/EB)staining and analyzed by fluorescence microscopy.Events like cell cycle arrest and apoptosis were observed by flow cytometric analysis and expressions of p-Rb,cyclin D1,cyclin-dependent kinase 4(CDK4),CDK6,Bax,Bcl-2,PI3 K/p-AKT were analyzed by immunoblot.Results:Carvacrol significantly reduced cell viability with the half maximal inhibitory concentration value of 200μmol/L at 24 and 48 h(P<0.05).importantly,there was a significant increase in the accumulation of the G0/G1 phase upon treatment with carvacrol in MCF-7 cells(P<0.05 or P<0.01).A remarkable decrease in protein expressions of p-Rb,cyclin D1,CDK4 and CDK6 denoted cell cycle arrest(P<0.05 or P<0.01).In addition,carvacrol treatment significantly inhibited PI3 K/p-AKT protein expressions leading to induction of apoptosis mediated by decreased Bcl2 and increased Bax protein expressions.Further,Annexin V/PI staining by FACS analysis,dual staining by AO/EB and PI staining studies suggested induction of apoptosis by carvacrol through PI3 K/Akt signaling pathway in MCF-7 cells.Conclusion:Carvacrol significantly inhibited the breast cancer MCF-7 cell proliferation and induced apoptosis via suppressing PI3/AKT signaling pathway.展开更多
基金support by AgriFutures Australia’s Chicken Meat Program[grant number PRJ-011584]is gratefully acknowledged.
文摘Background Broiler chickens are most vulnerable immediately after hatching due to their immature immune systems,making them susceptible to infectious diseases.The yolk plays an important role in early immune defence by showing relevant antioxidant and passive immunity capabilities during broiler embryonic development.The immunomodulatory effects of phytogenic compound carvacrol have been widely reported.After in ovo delivery in the amniotic fluid during embryonic development carvacrol is known to migrate to the yolk sac.However,it is unknown whether carvacrol in the yolk could enhance defence responsiveness in the yolk sac.Therefore,the aim of this study was to improve early immune function in chicken embryos,and it was hypothesized that in ovo delivery of carvacrol would result in immunomodulatory effects in the yolk sac,potentially improving post-hatch resilience.Methods On embryonic day(E)17.5,either a saline(control)or carvacrol solution was injected into the amniotic fluid.Yolk sac tissue samples were collected at E19.5,and transcriptomic analyses using RNA sequencing were performed,following functional enrichment analyses comparing the control(saline)and carvacrol-injected groups.Results The results showed that 268 genes were upregulated and 174 downregulated in the carvacrol group compared to the control(P<0.05;logFC<-0.5 or log FC>0.5).Functional analyses of these differentially expressed genes,using KEGG,REACTOME,and Gene Ontology databases,showed enrichment of several immune-related pathways.This included the pathways‘Antimicrobial peptides’(P=0.001)and‘Chemoattractant activity’(P=0.004),amongst others.Moreover,the‘NOD-like receptor signaling’pathway was enriched(P=0.002).Antimicrobial peptides are part of the innate immune defence and are amongst the molecules produced after the nucleotide oligomeriza-tion domain(NOD)-like receptor pathway activation.While these responses may be associated with an inflammatory reaction to an exogenous threat,they could also indicate that in ovo delivery of carvacrol could prepare the newly hatched chick against bacterial pathogens by potentially promoting antimicrobial peptide production through acti-vation of NOD-like receptor signaling in the yolk sac.Conclusion In conclusion,these findings suggest that in ovo delivery of carvacrol has the potential to enhance anti-pathogenic and pro-inflammatory responses in the yolk sac via upregulation of antimicrobial peptides,and NOD-like receptor pathways.
基金financial support from the National Natural Science Foundation of China(22278027)the Beijing Natural Science Foundation(2232013)。
文摘Practical application of carvacrol in different fields including foods and biopesticides has been limited due to its instability and water insolubility.In this work,carvacrol encapsulated Pickering emulsion is developed by using polymeric Janus nanoparticles as the stabilizer.To achieve this,dumbbell-shaped polymeric nanoparticles composed of two spheres of shellac and polylactic acid(PLA)are firstly prepared via co-precipitation in a rotating packed bed reactor,followed by grafting of chitooligosaccharides(COS)onto shellac to synthesis amphiphilic Janus nanoparticles(PLA/shellac-COS).Pickering emulsions with typical oil-in-water,bi-continuous structure and water-in-oil characteristics are produced by configuring carvacrol emulsions with different oil-to-water ratios.The stability of emulsions with 5%carvacrol content stabilized by 0.5% PLA/shellac-COS nanoparticles were more stable when compared to those prepared by shellac nanoparticles and PLA/shellac nanoparticles.After stored for one month,the carvacrol encapsulated Pickering emulsions maintained a high zeta potential of-43.8 mV,with no significant changes in particle size.These preliminary studies illustrated that polymeric Janus nanoparticles synthesized by co-precipitation in a rotating packed bed are promising particles for Pickering emulsions and related work in the future.
基金supported by Novus International Company(Missouri,USA)the earmarked fund for China Agricultural Research Systems(CARS-42)
文摘Background： In the post-antibiotic era, essential oils （EO） are promising alternatives to growth-promoting antibiotics. The aim of the present study was to investigate the antibacterial activities of an EO product and its components thymol and carvacrol in vitro, and the efficacy of EO to control Clostridium perffingens challenge in broiler chickens. Results： The in vitro minimum inhibitory concentration assay showed strong antibacterial activity of the EO product, thymol, and carvacrol against pathogenic Escherichia col~, C. perffingens, and Salmonella strains, and weak activity towards beneficial Lactobacillus strains. Besides, an additive effect was observed between thymol and carvacrol. The in vivo study was carried out with 448 male broiler chicks following a 4 x 2 factorial arrangement to test the effects of EO supplementation （0, 60, 120, or 240 mg/kg EO in wheat-based diet）, pathogen challenge （with or without oral gavage of C. perfr/ngens from day 14 to day 20） and their interactions. Each treatment consisted of eight replicate pens （seven birds/pen）. The challenge led to macroscopic gut lesions, and resulted in a significant increase in ileal populations of C. perffingens and Eschefichia subgroup （P ≤ 0.05） on day 21. Dietary EO supplementation did not influence C. perfringens numbers, but linearly alleviated intestinal lesions on day 21 and 28 （P= 0.010 and 0.036, respectively）, and decreased Eschefichia populations in ileum with increased EO dosages （P = 0.027 and 0.071 for day 21 and 28, respectively）. For caecum, EO quadratically influenced Lactobacillus populations on day 21 （P = 0.002）, and linearly decreased the numbers of total bacteria and Eschefichio on day 28 （P = 0.026 and 0.060, respectively）. Mean thymol and carvacrol concentrations in the small intestine were 0.21 and 0.20 μg/g in intestinal digesta （wet weight）, respectively, for birds fed 60 mg/kg EO, and 0.80 and 0.71 μg/g, respectively, for birds fed 240 mg/kg EO. Conclusions： These results indicated that dietary EO supplementation could affect intestinal microbiota and alleviate intestinal lesions in broilers, which may contribute in controlling C. perffingens infection in broiler chickens.
基金supported by Hubei Province Science and Technology Innovation Major Project(2019ABA081,2021BBA083)China Agriculture Research System of MOF and MARA.
文摘Background:Over the past years,antibiotic growth promoter had been restricted in animal husbandry production in many countries because of antimicrobial resistance and foodborne antibiotic residues.However,the problems of poor intestinal health and low growth efficiency of piglets have not been solved completely in an antibiotic-free diet,and it is urgent to explore alternatives to antimicrobial growth promoters.Methods:Here,a total of 532 weaned pigs were assigned to one of 4 treatments,the low amino acid(AA)level diet(d 1 to d 14 is 1.35%,d 15 to d 42 is 1.25%)(Low AA),the low AA level diet supplementation with a carvacrol-thymol blend(50 mg carvacrol and 50 mg thymol/kg of diet)(CB)(Low AA+CB),the high AA level diet(d 1 to d 14 is 1.50%,d 15 to d 42 is 1.40%)(High AA),and the high AA level diet supplementation with a CB(High AA+CB),respectively.Then we measured growth performance and intestinal health indicators of weaned pigs.Results:Results showed that high AA level significantly reduced plasma urea nitrogen,plasma Interleukin-6(IL-6)and fecal lipocalin-2 contents(P<0.05),significantly increased the relative abundance of fecal Lactobacillus and Enterococcus,and had a trend to increase the fecal secretory immunoglobulin A(sIgA)and mucin 2(MUC 2)contents(P<0.05)in piglets,thereby alleviating the diarrhea of piglets and reducing the feed conversion ratio(FCR)of piglets during d 1~14 after weaning.Dietary supplementation with CB significantly increased the activity of plasma antioxidant enzymes T-SOD and GSH-px(P<0.05),while significantly reduced plasma malondialdehyde(MDA),plasma interleukin-1β(IL-1β),plasma endotoxin and D-lactic acid contents(P<0.05).Meanwhile,CB significantly decreased fecal lipocalin-2 contents and the abundance of fecal Escherichia coli(P<0.05).Thus,we hypothesis that dietary supplementation with CB significantly increased the average daily gain(ADG)of piglets(P<0.05)during d 1~14 after weaning through promoting intestinal health.Conclusion:These results suggest that high AA level and dietary supplementation with CB improved the growth performance of weaned pigs in an antibiotic-free diet by improving AA metabolism and intestinal antioxidant capacity.
文摘Objective:To unravel the mechanism of anti-inflammatory activity of carvacrol in D-galactosamine(D-GalN)-induced hepatotoxic rats.Methods:The mRNA and protein expression levels of tumor necrosis factor-alpha(TNF-α).interleukin-6[IL-6).inducible nitric oxide synthase(iNOS),cyelooxy genase-2(COX-2) and nuclear faclor kappa-B(NF-κB) were assayed by semi-quantitative reverse transcriptase polymerase chain reaction(RTPCR) and western blot analysis.Results:We found that the mRNA and protein expressions of TNF-α. IL-6,iNOS,COX-2 and NF-κB were significanlly up-regulated in D-galactosamine induced hepatotoxic rats and treatment with carvacrol significantly down-regulated the expressions of these genes showing the mechanism behind the anti-inflammatory activity of carvacrol. Conclusions:All above results reveal that the carvacrol well known anti-inflammatory activities in D-galactosamine induced hepatotoxic rats.
文摘Medicinal plants are rich in nutrients and phytochemicals which prevent and treat a wide range of ailments.Accumulating experimental studies exhibit that some bioactive ingredients extracted from medicinal plants have suitable therapeutic effects on hepatic and renal injuries.This review focuses on the hepato-and reno-protective effects of thymoquinone,crocin,and carvacrol.The relevant literature was retrieved from PubMed,Scopus,Web of Science,and Google Scholar databases from the beginning of 2015 until the end of November 2021.According to the scientific evidence,the considered phytochemicals in this review have been applied with useful therapeutic effects on hepatic and renal damage.These therapeutic effects were mainly mediated through the amelioration of oxidative stress,suppression of inflammatory responses,and inhibition of apoptosis.Intracellular signaling pathways linked to nuclear factor kappa B(NF-κB),adenosine monophosphate-activated protein kinase,c-jun N-terminal kinase,and extracellular signal-regulated kinase 1/2 and Toll-like receptors are the most important pathways targeted by these phytochemicals.Up-regulation of transcription factor Nrf2 and down-regulation of transforming growth factor-beta 1 by these natural compounds also contribute to the alleviation of hepatic and renal injuries.
文摘Nine new sulfonamides derived from carvacrol were prepared through a reaction between 4-hydroxy-2-isopropyl-5-methyl benzene sulfonyl chloride with various amines in excellent yields (76% - 92%). The sulfonamides were characterized using spectrometric and spectroscopic methods. Among these compounds, three derivatives showed excellent results in antibacterial activity against resistant S. aureus strains, with MIC values ranging from 3.9 to 62.50 ppm. The sulfonamide derivative of 4-methylaniline (SULF-1) had the best performance for all tested strains of bacteria (MIC = 3.9 to 15.62 ppm). Furthermore, the sulfonamide derivative of 4-fluoro aniline (SULF-3), which also presented promising results, was found to have a synergistic effect when combined with tetracycline and partial synergistic effect when combined with ampicillin, exhibiting an FIC index between 0.50 and 0.75. The sulfonamide derivative of 4-methylaniline had a synergistic effect in combination with erythromycin exhibiting an FIC index of 0.37. Carvacrol in association with the antibiotics tested did not have a synergistic effect.
文摘The aim of this study was to elucidate the mode of action of two antimicrobial compounds, nisin, which is hydrophilic, and the lipophilic compound carvacrol. For that purpose, both antimicrobials were loaded into Oil-in-/Water nanoemulsions, and their antimicrobial activity against Bacillus subtilis was investigated. The experimental results indicated that the O/W nanoemulsions loaded with both, nisin and carvacrol, having Sauter mean diameter （d3,2） of around 120 nm, were considerably stable under the conditions investigated （up to 1 week storage at room temperature）. The interfacial tension between nisin aqueous solutions and soybean oil could be reduced up to 12 mN/m, as compared to that of pure water and soybean oil （21 mN/m）, so that demonstrating the potential of nisin to be used as emulsifier. The results obtained for O/W nanoemulsions loaded with both antimicrobial compounds indicated a reduction of nearly two log cycles （around 100 times） on B. subtilis population upon incubation for 24 h, compared to the blank （10 mM phosphate buffer）.
文摘The product of plant essential oil, oregano carvacrol, was added in the diet of broiler, to study the effect of oregano carvacrol on growth performance of broilers. Using single factor test, 190 663 individuals of healthy AA broilers at 0 day old were randomly divided into two groups, eight repeats in control group and four repeats in experimental group. The broilers in control group were fed with basal diet （containing 10 mg/kg cnramycin and 60 mg/kg salinomycin sodium）, and those in experimental groups were fed with the basal diet adding with 100 mg/kg oregano carvacrol. Compared with control group, the daily gain of broilers in exper- imental group was increased noticeably by 3.41 g/broiler （P 〈 0. 05） ; the slaughter quality was increased remarkably by 143 g/broiler （P 〈 0.05 ） ; the survival rate was increased significantly by 3.01% （ P 〈 0.05 ） ; and the feed gain ratio was reduced markedly by 0.08 （ P 〈 0.05 ）. The paper provided a theoretical basis for application of oregano carvacrol in broiler production.
基金All experimental procedures pursued the relevant guidelines and regulations of the National Institute of Health Guide for the Care and Use of Laboratory Animals(NIH Publications No.80-23,revised 1978)were approved by the Ethics Committee of Mashhad University of Medical Sciences,Iran(IR.MUMS.fm.REC.1397.139).
文摘Background:Zataria multiflora and carvacrol showed various pharmacological prop-erties including anti-inflammatory and anti-oxidant effects.However,up to now no studies have explored its potential benefits in ameliorating sepsis-induced aortic and cardiac injury.Thus,this study aimed to investigate the effects of Z.multiflora and carvacrol on nitric oxide(NO)and oxidative stress indicators in lipopolysaccharide(LPS)-induced aortic and cardiac injury.Methods:Adult male Wistar rats were assigned to:Control,lipopolysaccharide(LPS)(1 mg/kg,intraperitoneal(i.p.)),and Z.multiflora hydro-ethanolic extract(ZME,50–200 mg/kg,oral)-and carvacrol(25–100 mg/kg,oral)-treated groups.LPS was in-jected daily for 14 days.Treatment with ZME and carvacrol started 3 days before LPS administration and treatment continued during LPS administration.At the end of the study,the levels of malondialdehyde(MDA),NO,thiols,and antioxidant enzymes were evaluated.Results:Our findings showed a significant reduction in the levels of superoxide dis-mutase(SOD),catalase(CAT),and thiols in the LPS group,which were restored by ZME and carvacrol.Furthermore,ZME and carvacrol decreased MDA and NO in car-diac and aortic tissues of LPS-injected rats.Conclusions:The results suggest protective effects of ZME and carvacrol on LPS-induced cardiovascular injury via improved redox hemostasis and attenuated NO pro-duction.However,additional studies are needed to elucidate the effects of ZME and its constituents on inflammatory responses mediated by LPS.
基金the Canakkale Onsekiz Mart University Research Fund for financial assistance(the project number COMU BAP FDK-2013-102)and Adnan COBAN for providing research facilities.
文摘The present study investigated the effects of phytoadditive carvacrol on growth performance, feed utilization, hematological, non-specific immune and serum biochemical parameters in rainbow trout (Oncorhynchus mykiss). In trial, 240 rainbow trout which had average weight of 10.79 ± 0.57 was used. Fish were divided into four groups before being fed for 60 days with 0, 1 (C1), 3 (C3) or 5 (C5) g/kg of carvacrol. There were no particular differences in the growth performance, feed utilization, whole body composition, hematological parameters between the experimental group and the control group. However, as compared to control group, a significant increase of serum lysozyme activity was seen in groups receiving feed containing C3 and C5 groups after 30 days. The myeloperoxidase activity in groups (C1 and C3) was significantly higher on the 30th day of sampling. Myeloperoxidase activity of C5 treated group was significantly higher on the 60th day in comparison with the control group. Serum glucose levels significantly decreased on 60th day for C1, C3 and C5 treated groups. The serum total protein, globulin and triglyceride levels of C3 treated groups was significantly higher on the 60th day in comparison with the control and other carvacrol treated groups. The level of serum cholesterol in C5 treated groups was significantly lower on the 30th day in comparison with the control and other carvacrol treated groups. The results of the present study indicated that dietary supplementation of 3 g/kg of carvacrol in commercial diets could improve some non-specific and biochemical status in rainbow trout.
基金Supporting Project number(PNURSP 2025R31)Princess Nourah bint Abdulrahman University,Riyadh,Saudi Arabiathe Deanship of Scientific Research(DSR)at King Faisal University under project no.[KFU250265].
文摘The virulence factors including biofilm and virulence proteins in Listeria monocytogenes(L.monocytogenes)threatens human health.Herein,the inhibitory mechanisms of carvacrol(CAR)on the biofilm formation and virulence production in L.monocytogenes were explored.It was found the minimum biofilm inhibition concentration of CAR was 0.8 mg/mL.CAR could effectively inhibit bacterial biofilm formation by reduction of extracellular polymeric substance,including extracellular polysaccharides,proteins,and DNA.CAR could interfere with the bacterial swarming and swimming motilities,thereby hindering biofilm development.Hemolytic capability of Listeriolysin O was significantly decreased from 85.75%to 28.13%after treatment with CAR at the minimum inhibitory concentration(MIC).Transcriptomic analysis revealed exposure to 1/2 MIC of CAR resulted in 969 differentially expressed genes,with downregulation of virulence factor-related genes.CAR might bind to AgrB within the quorum sensing system,thereby affecting the biofilm formation regulation and virulence expression.In addition,CAR exhibited efficient inhibitory effects on the biofilm formation on different food contacting materials and vegetable surfaces.These findings provided new insights into biofilm-inhibition and virulence-reduction mechanisms of CAR on L.monocytogenes,suggesting potential applications in controlling L.monocytogenes biofilm contamination in food industries.
文摘The use of natural products as antibiotic adjuvants to enhance efficacy and mitigate resistance is increasingly recog-nized as a promising strategy.This study explored five novel synergistic antimicrobial combinations(SACs)of carvac-rol(CARV)and three already identified SACs of thymol(THY)with chloramphenicol,gentamicin,and streptomycin against Staphylococcus aureus and Acinetobacter baumannii,critical WHO-listed pathogens,and investigated their mechanisms of action and resistance-prevention capabilities.Despite being isomers,CARV and THY exhibited distinct synergistic effects and fractional inhibitory concentration index(FICI)values depending on the antibiotic and bac-terial species.The SACs significantly reduced the required antibiotic dose by 4-to 16-fold,with FICI values ranging from 0.25 to 0.5.Growth kinetics revealed that SACs completely inhibited planktonic bacterial growth,outperform-ing antibiotics alone.Additionally,the SACs demonstrated efficacy in both inhibiting and eradicating biofilms of S.aureus and A.baumannii.Resistance development studies highlighted that neither THY nor CARV induced resistance in these pathogens.Moreover,SACs combining aminoglycosides with THY reduced the emergence of resistance in A.baumannii by up to 32-fold.In S.aureus,THY mitigated gentamicin resistance by 16-fold.CARV exhibited similar,albeit slightly less potent,effects.Mechanistic investigations revealed that THY and CARV exert antimicrobial action by multiple mechanisms,includ-ing bacterial membrane depolarization and disruption,efflux pump inhibition,disrupting ATP metabolism and miti-gating oxidative stress induced by antibiotics.These findings highlight the potential of SACs to enhance antibiotic efficacy while preventing resistance,positioning them as strong candidates for innovative antimicrobial therapies against multidrug-resistant pathogens.
基金supported by the National Natural Science Foundation of China(32272445)the Shenzhen Natural Science Foundation(JCYJ20230807111500001 and JCYJ20240813151818024)+1 种基金the Natural Science Foundation of Guangdong Province of China(2023A1515012728)the Deanship of Scientific Research(DSR)at King Faisal University under project no KFU250674.
文摘In this study,carvacrol nanoemulsion(CN)was prepared using ultrasonic emulsification and the synergistic bactericidal effect of ultrasound(US)and CN on Listeria monocytogenes and its mechanism were investigated.The results showed that the combined treatment of US and CN had a significant(P<0.05)synergistic effect on the inactivation of L.monocytogenes,with synergistic values(SV)of 1.345 and 1.325,respectively.US+CN disrupts mononucleosis cell membranes and increases their permeability,as demonstrated by confocal laser scanning microscopy,flow cytometry and cell content release assays.N-phenyl-1-naphthylamine,reactive oxygen species and malondialdehyde assays showed that the combined treatment accelerated cellular oxidative stress and peroxidation,thereby affecting membrane lipid homeostasis.Field emission scanning electron microscopy showed that US+CN caused cell membranes to dry out and crumble.In addition,US+CN effectively kill L.monocytogenes on the surface of cabbages,reduce the weight loss of cabbages during storage,and had no significant(P>0.05)effect on its color and hardness.This study demonstrated that US+CN has the potential to inactivate foodborne pathogens and improve the safety of fresh produce,providing theoretical possibilities for its application.
基金supported by National Natural Science Foundation of China(Grant no.32202004)the Program for Scientific Research Innovation Team of Young Scholar in Colleges and Universities of Shandong Province(Grant no.2023KJ241)+1 种基金Shandong Provincial Natural Science Foundation(Grant no.ZR2021QC214)the Taif University Researchers Supporting Project(Grant no.TURSP-HC2024/12),Saudi Arabia.
文摘The contamination of L.monocytogenes on different foods mainly achieves by the formation of biofilm,and L.monocytogenes biofilm poses great risks to food industry.Hence,exploring the safe and nontoxic substances to interfere and destroy L.monocytogenes biofilm is of great concern.In this research,carvacrol was selected as the antibiofilm substance to investigate its effect on the three-dimensional structure,extracellular polymer matrix,aggregation,adhesion and Agr quorum sensing system of L.monocytogenes biofilm.The results revealed that L.monocytogenes showed the greatest biofilm formation ability in 5 days cultivation on stainless steel sheet.The minimum biofilm inhibition concentration and minimum biofilm elimination concentration of carvacrol against L.monocytogenes biofilm were 0.4 and 0.8 mg/mL.SEM and fluorescent pictures demonstrated that carvacrol could destroy and eliminate polymer matrix of L.monocytogenes biofilm.Furthermore,carvacrol could impact the swimming motility and aggregation ability of L.monocytogenes to inhibit the adhesion and initial formation of biofilm.Finally,carvacrol also could effectively inhibit the secretion and production of exopolysaccharide and proteins,downregulating the gene expression and binding with signaling proteins in Agr quorum sensing system to interfere the formation of biofilm.In conclusion,carvacrol could be applied as the ideal antibiofilm substance to inhibit and destroy L.monocytogenes biofilm in food industry.
文摘Objective:To entrap carvacrol(CAR)in bovine serum albumin nanoparticles(BSANPs)to form CAR-loaded BSANPs(CAR@BSANPs)and to explore the anti-cancer effects in breast adenocarcinoma cells(MCF-7 cells)treated with CAR and CAR@BSANPs.Methods:A desolvation method was used to synthesize BSANPs and CAR@BSANPs.The BSANPs and CAR@BSANPs were characterized by several physicochemical methods,including visual observation,high-resolution field emission scanning electron microscopy,Fourier transform infrared spectroscopy,and high-performance liquid chromatography.MCF-7 cells were used and analyzed after 24 h of exposure to CAR and CAR@BSANPs at half-maximal inhibitory concentration.The anti-proliferative,apoptotic,reactive oxygen species(ROS),and nitric oxide(NO)scavenging activity as well as gene expression analysis were investigated by the cell viability assay,phase-contrast microscopy,2',7'-dichlorofluorescein-diacetate assay,Griess-Illosvoy colorimetric assay,and quantitative real-time polymerase chain reaction,respectively.Results:CAR and CAR@BSANPs showed anti-proliferative,apoptotic,ROS generation,and NO scavenging effects on MCF-7 cells.Expression profile of B-cell lymphoma 2-like 11(BCL2L11),vascular endothelial growth factor A(VEGFA),hypoxia inducible factor factor-1α(HIF1A),BCL2L11/apoptosis regulator(BAX),and BCL2L11/Bcl2 homologous antagonist/killer 1(BAK1)ratios revealed downregulated genes;and BAX,BAK1,and CASP8 were upregulated by CAR and CAR@BSANPs treatment.In vitro anticancer assays of the CAR and CAR@BSANPs showed that CAR@BSANPs demonstrated higher therapeutic efficacy in the MCF-7 cells than CAR.Conclusions:CAR and CAR@BSANPs affect gene expression and may subsequently reduce the growth and proliferation of the MCF-7 cells.Molecular targeting of regulatory genes of the MCF-7 cells with CAR and CAR@BSANPs may be an effective therapeutic strategy against breast cancer.
基金supported by the National Natural Science Foundation of China(No.81902701 to Y.Li,82103649 to H.Zhang)lthe Natural Science Foundation of Liaoning Province,China(No.20180530037,2023JH2/20200036 to Y.Li,2022-MS-183 to W.Dai).
文摘Poor prognosis is associated with oral squamous cell carcinoma(OSCC),an aggressive form of malignant tumor.1 This study aimed to investigate the pharmacological effects of carvacrol on OSCC by targeting the tumor-associated antigen FGFR1.As a key survival biomarker in OSCC,FGFR1 plays a crucial role in malignant transformation.Carvacrol,a specific FGFR1 inhibitor,induces its degradation via the ubiquitin-proteasome pathway.
基金support in the form of Senior Research Fellowship (SRF)。
文摘Objective:To examine the role of carvacrol in modulating PI3 K/AKT signaling involved in human breast cancer pathogenesis using in vitro experimental model MCF-7 cells.Methods:MTT and lactate dehydrogenase assays were performed with cells treated with different doses of carvacrol(0–250μmol/L)at different time points(24 and 48 h).The nuclear morphology was assessed in MCF-7 cells with propidium iodide(PI)and acridine orange/ethidium bromide(AO/EB)staining and analyzed by fluorescence microscopy.Events like cell cycle arrest and apoptosis were observed by flow cytometric analysis and expressions of p-Rb,cyclin D1,cyclin-dependent kinase 4(CDK4),CDK6,Bax,Bcl-2,PI3 K/p-AKT were analyzed by immunoblot.Results:Carvacrol significantly reduced cell viability with the half maximal inhibitory concentration value of 200μmol/L at 24 and 48 h(P<0.05).importantly,there was a significant increase in the accumulation of the G0/G1 phase upon treatment with carvacrol in MCF-7 cells(P<0.05 or P<0.01).A remarkable decrease in protein expressions of p-Rb,cyclin D1,CDK4 and CDK6 denoted cell cycle arrest(P<0.05 or P<0.01).In addition,carvacrol treatment significantly inhibited PI3 K/p-AKT protein expressions leading to induction of apoptosis mediated by decreased Bcl2 and increased Bax protein expressions.Further,Annexin V/PI staining by FACS analysis,dual staining by AO/EB and PI staining studies suggested induction of apoptosis by carvacrol through PI3 K/Akt signaling pathway in MCF-7 cells.Conclusion:Carvacrol significantly inhibited the breast cancer MCF-7 cell proliferation and induced apoptosis via suppressing PI3/AKT signaling pathway.
