摘要
为探究香芹酚乙酸酯(Carvacrol acetate,CAA)对糖尿病脑病(Diabetic encephalopathy,DE)的保护作用,构建了SD大鼠的DE动物模型。将雄性SD大鼠随机分为正常组、糖尿病模型组、胰岛素给药组、依达拉奉给药组以及CAA低、中、高剂量给药组。除正常对照组外,给所有大鼠尾静脉注射链脲佐菌素,以构建糖尿病模型。稳定培养4周后持续给药4周,开展水迷宫实验和旷场实验来检测大鼠记忆能力和运动能力,通过检测活性氧(Reactive oxygen species,ROS)水平、丙二醛(Malondialdehyde,MDA)水平、超氧化物歧化酶(Superoxide dismutase,SOD)活力变化及核因子红细胞-2-相关因子2(Nuclear factor-E2-related factor2,Nrf2)蛋白印迹来分析CAA保护作用与氧化应激的关系。研究结果表明:CAA可以有效改善DE大鼠的记忆能力,提高其运动探索能力,对其神经细胞有保护作用,且这种保护作用具有剂量依赖性,其中高剂量CAA的治疗效果最佳;CAA可以降低ROS和脂质氧化水平,促进Nrf2入核,激活下游抗氧化酶,改善机体氧化应激。
An animal model of Diabetic encephalopathy was established in SD rats to investigate the protective effect of Carvacrol acetate(CAA)on Diabetic encephalopathy.Male SD rats were randomly assigned into the control group,the diabetes model group,the insulin administration group,the edaravone administration group,as well as the low-,medium-,and high-dose CAA administration groups.Streptozotocin was injected into the tail vein of the rats in the diabetes model group and all administration groups to induce diabetes.After the hyperglycemic rats were maintained in a stable state for 4 weeks,corresponding drugs were continuously administered for another 4 weeks.Subsequently,the Morris water maze experiment and the open field test were conducted to evaluate the memory and motor abilities of the rats.The correlation between the protective effect of CAA and oxidative stress was analyzed by investigating the changes in ROS and MDA levels,SOD activity,along with the Western blot of nuclear Nrf2 protein.The results showed that:CAA could effectively enhance the learning and memory functions of rats with Diabetic encephalopathy,improve their motor exploration ability,and exert a protective effect on nerve cells.Moreover,this protective effect was dose-dependent,with the high-dose CAA showing a better treatment effect.CAA was capable of reducing the levels of ROS and lipid oxidation,facilitating the nuclear translocation of Nrf2,activating downstream antioxidant enzymes,and thus improving the body's oxidative stress status.
作者
宋英
熊和
马启壮
SONG Ying;XIONG He;MA Qizhuang(College of Pharmaceutical Science,Zhejiang University of Technology,Hangzhou 310014,China)
出处
《浙江工业大学学报》
北大核心
2026年第2期215-220,共6页
Journal of Zhejiang University of Technology
基金
国家自然科学基金资助项目(82174038)
浙江省自然科学基金资助项目(LD22H090002)。
关键词
糖尿病脑病
氧化应激
香芹酚乙酸酯
Diabetic encephalopathy
oxidative stress
Carvacrol acetate
