In Candida species,the endoplasmic reticulum(ER)stress response—regulated by the unfolded protein response(UPR)—serves as a critical adaptive mechanism affecting both pathogenicity and antifungal resistance.This rev...In Candida species,the endoplasmic reticulum(ER)stress response—regulated by the unfolded protein response(UPR)—serves as a critical adaptive mechanism affecting both pathogenicity and antifungal resistance.This review aims to synthesize current knowledge on ER stress pathways in Candida glabrata and Candida albicans,highlighting their species-specific adaptations and therapeutic implications.We systematically analyzed peer-reviewed literature on ER stress mechanisms in Candida,focusing on comparative studies of UPR signaling.Emphasis was placed on C.glabrata’s inositol-requiring enzyme 1(IRE1)-dependent Regulated IRE1-Dependent Decay(RIDD)pathway and C.albicans’IRE1/HAC1 and calcium-mediated pathways.Connections to virulence and drug resistance were evaluated through genetic,transcriptomic,and phenotypic evidence.Candida species employ divergent UPR strategies:C.glabrata mitigates ER stress primarily via RIDD-mediated mRNA decay to reduce protein load,while C.albicans enhances folding capacity through HAC1 splicing and calcium homeostasis.These adaptations promote survival in hostile host environments(e.g.,oxidative stress,immune attacks)and are linked to resistance against azoles and echinocandins.Pharmacological disruption of UPR components(e.g.,IRE1 inhibitors)sensitizes Candida to antifungals in experimental models.ER stress response pathways are promising targets for antifungal drug development.Understanding species-specific UPR mechanisms in Candida could guide novel therapies to overcome resistance and improve treatment outcomes.展开更多
BACKGROUND Emerging evidence implicates Candida albicans(C.albicans)in human oncogenesis.Notably,studies have supported its involvement in regulating outcomes in colorectal cancer(CRC).This study investigated the para...BACKGROUND Emerging evidence implicates Candida albicans(C.albicans)in human oncogenesis.Notably,studies have supported its involvement in regulating outcomes in colorectal cancer(CRC).This study investigated the paradoxical role of C.albicans in CRC,aiming to determine whether it promotes or suppresses tumor development,with a focus on the mechanistic basis linked to its metabolic profile.AIM To investigate the dual role of C.albicans in the development and progression of CRC through metabolite profiling and to establish a prognostic model that integrates the microbial and metabolic interactions in CRC,providing insights into potential therapeutic strategies and clinical outcomes.METHODSA prognostic model integrating C. albicans with CRC was developed, incorporating enrichment analysis, immuneinfiltration profiling, survival analysis, Mendelian randomization, single-cell sequencing, and spatial transcriptomics.The effects of the C. albicans metabolite mixture on CRC cells were subsequently validated in vitro. Theprimary metabolite composition was characterized using liquid chromatography-mass spectrometry.RESULTSA prognostic model based on five specific mRNA markers, EHD4, LIME1, GADD45B, TIMP1, and FDFT1, wasestablished. The C. albicans metabolite mixture significantly reduced CRC cell viability. Post-treatment analysisrevealed a significant decrease in gene expression in HT29 cells, while the expression levels of TIMP1, EHD4, andGADD45B were significantly elevated in HCT116 cells. Conversely, LIME1 expression and that of other CRC celllines showed reductions. In normal colonic epithelial cells (NCM460), GADD45B, TIMP1, and FDFT1 expressionlevels were significantly increased, while LIME1 and EHD4 levels were markedly reduced. Following metabolitetreatment, the invasive and migratory capabilities of NCM460, HT29, and HCT116 cells were reduced. Quantitativeanalysis of extracellular ATP post-treatment showed a significant elevation (P < 0.01). The C. albicans metabolitemixture had no effect on reactive oxygen species accumulation in CRC cells but led to a reduction in mitochondrialmembrane potential, increased intracellular lipid peroxidation, and induced apoptosis. Metabolomic profilingrevealed significant alterations, with 516 metabolites upregulated and 531 downregulated.CONCLUSIONThis study introduced a novel prognostic model for CRC risk assessment. The findings suggested that the C.albicans metabolite mixture exerted an inhibitory effect on CRC initiation.展开更多
Background: Vulvovaginal candidiasis (VVC) is a common cause of significant morbidity, affecting millions of women worldwide. It is estimated that approximately 75%of women of childbearing age will have at least one e...Background: Vulvovaginal candidiasis (VVC) is a common cause of significant morbidity, affecting millions of women worldwide. It is estimated that approximately 75%of women of childbearing age will have at least one episode of candidiasis in their lifetime. In the last decades, resistance to azoles has become a public health problem. Although studies on vulvovaginitis have been done, there is lack of VVC studies in our area. The aim of this study was to describe the etiological and resistance profiles of vulvovaginal candidiasis to standard antifungus at the Saint Camille Hospital of Ouagadougou (HOSCO), Burkina Faso. Methods: We conducted a prospective study from January 2018 to December 2022. From vulvovaginal swabs, Candida species were identified using the ChromID® Candida Agar medium and the API® Candida gallery. Antifungal susceptibility testing was performed using Kirby-Bauer agar disk diffusion. Results: A total of 4789 women were sampled. The average age of sexually active women was 27.80+/−6.77 years, with extremes ranging from 15 to 64 years. Vaginal Candida infections accounted for 74.16% of the cases. The 20 - 29 age group was the most affected by vulvovaginal candidiasis. Pregnant women accounted for 28.76% of our study population. Women in the second (2nd) trimester of pregnancy had more Candida infections. Candida albicans was the most isolated species (55.12%), followed by Candida glabrata (27.64%), Candida tropicalis (6.91%), Candida famata (6.67%), Candida krusei (2.56%). All the Candida species isolated showed very high of resistance to Fluconazole (45.2%), Miconazole (23.7%) and Clotrimazole (45.7%). Conclusion: Species-specific antifungal results should always be considered to avoid antifungal resistance associated with vulvovaginal candidiasis. Identifying the causative species using vaginal fungal cultures can help guide therapy and improve outcomes for these patients.展开更多
This study examined the EAG(Electroantennogram)responses of Stilpnotia candida to a mix of host plant volatiles and to provide a foundation for the development of plant-derived attractants.During the peak period of ad...This study examined the EAG(Electroantennogram)responses of Stilpnotia candida to a mix of host plant volatiles and to provide a foundation for the development of plant-derived attractants.During the peak period of adult eclosion,gas chromatography–mass spectrometry analyzed and identified the volatiles emitted by Populus×beijingensis found in Xizang.Based on the preliminary EAG experiments and the GC‒MS results,a blending scheme was developed.EAG and Y-tube-olfactometry were employed to measure the electrophysiological and behavioural responses of unmated males and females 24 h after eclosion 10 blends of volatiles derived from five host plants.The GC–MS analysis revealed 22 volatile compounds from Populus×beijingensis leaves,composed of esters,hydrocarbons,terpenoids,alcohols,phenols,and ether.The results indicated that all 10 blending schemes produced EAG responses in mature S.candida.The concentration thresholds were between 1 and 10μg·μL^(-1),above the optimal concentration,and a corresponding decrease in EAG was observed.According to intergroup comparisons,mature S.candida had more pronounced EAG responses.Under different concentrations,there were significant differences in the EAG from male and female S.candida to each blending scheme.Behavioural response tests indicated that schemes 2,7,and 8 exhibited significantly greater attractiveness to adult S.candida.The combined results from the EAG and behavioral response experiments demonstrated that unmated male and female adult S.candida have varying degrees of sensitivity to the volatile compounds from the 10 blending schemes specific to Xizangan Populus×beijingensis.Schemes 2,7,and 8 showed robust EAG responses and attractive behavioural responses to both male and female adult S.candida.展开更多
A reliable estimation of Candida bloodstream infection prevalence is increasingly important to track changes in Candida species distribution and define burden of ongoing candidemia.A systematic review and meta-analysi...A reliable estimation of Candida bloodstream infection prevalence is increasingly important to track changes in Candida species distribution and define burden of ongoing candidemia.A systematic review and meta-analysis were conducted to estimate candidemia prevalence and identify patterns of Candida species in South-eastern Asia.Systematic electronic-databases literature search was performed on published studies recorded candidemia prevalence in South-Eastern Asia.Using meta-analysis of proportions,the overall pooled prevalences of candidemia by Candida(C.)albicans,C.tropicalis,C.parapsilosis and C.glabrata were calculated as 28.4%(95%CI 24.9-31.8),29.2%(95%CI 24.7-33.7),19.1%(95%CI 14.8-23.4)and 14.0%(95%CI 10.4-17.5),respectively.Based on publication year and country,subgroup analyses were conducted on Candida species to determine heterogeneity source.The findings may not precisely reflect true candidemia prevalence in different countries.Therefore,it highlights continuous need to conduct prevalence studies,assess and monitor growing burden,control effect of potential risk factors and implement regional surveillance programs to prevent further rise.展开更多
Background:Houttuynia cordata injection(HCI),made from fresh Houttuynia cordata,exerts heat-clearing,detoxifying and diuretic effects.It is indicated for various infections including lung abscess,fever,leucorrhea,urin...Background:Houttuynia cordata injection(HCI),made from fresh Houttuynia cordata,exerts heat-clearing,detoxifying and diuretic effects.It is indicated for various infections including lung abscess,fever,leucorrhea,urinary tract infection and carbuncle.Candida albicans is a common opportunistic pathogen in immunocompromised individuals.This pathogenic fungus colonizes in skin,mucosa membrane and digestive tract,potentially progressing from localized mucosal infections to systemic disease.Methods:The minimum inhibitory concentration(MIC)of HCI against C.albicans strain was determined by the microdilution method.The hyphal status of C.albicans was observed after incubation in the liquid medium and evaluated by Gram staining.The biofilm formation ability was measured using XTT reduction assay and assessed by Calcofluor White staining.The expression of virulence-related genes was detected with quantitative RT-PCR.Results:The MIC of HCI against C.albicans strain was determined to be 0.5 g/mL.At this concentration,HCI exhibited inhibitory effects on hyphal formation,as confirmed by both liquid medium observation and Gram staining.HCI at the MIC also effectively inhibited C.albicans biofilm formation,which was verified through XTT reduction assay and Calcofluor White staining.Additionally,gene expression analysis revealed that HCI significantly suppressed the expression of virulence-related genes in C.albicans.Conclusion:HCI demonstrates inhibitory effects on C.albicans growth and biofilm development.It inhibits hyphal formation,affecting the yeast-to-hyphal transition.This study investigated the antifungal effects of HCI,providing potent experimental evidence for its mechanism of action against C.albicans.展开更多
Candida albicans(C.albicans)represents one of the most prevalent opportunistic fungal pathogens in cancer patients.Although the association between C.albicans and cancer has been recognized for decades,the causal rela...Candida albicans(C.albicans)represents one of the most prevalent opportunistic fungal pathogens in cancer patients.Although the association between C.albicans and cancer has been recognized for decades,the causal relationship,whether C.albicans infection is a consequence of cancer or a direct contributor to cancer development-remains a subject of intensive investigation.Recently,the complex interplay between microbes and cancer has garnered significant attention within the scientific community,with growing interest in elucidating the underlying molecular mechanisms.This review systematically examines the biological characteristics of C.albicans,its multifaceted interactions with the host,and its relationship with the intestinal microbiota.Additionally,it provides a comprehensive analysis of the association between C.albicans and the development of various malignancies,with particular emphasis on digestive tract cancers.The review also identifies critical knowledge gaps and apparent contradictions in existing research,highlighting potential avenues for breakthroughs that will advance the efficient and accurate screening,diagnosis,and treatment of cancer.展开更多
Candida albicans(C.abicans),вcommon pathogenic fungus in nature,has enough cæpity to cause Severe brain infection through various mesns under immunocompromised conditions.Currently,stablishing a basic animal dis...Candida albicans(C.abicans),вcommon pathogenic fungus in nature,has enough cæpity to cause Severe brain infection through various mesns under immunocompromised conditions.Currently,stablishing a basic animal disesse model has become the main rsrch tool,which isconducive to simulat ing fungal encephalitis effectively.However,the widely used bloodbarne infection model established by intravenoOus(I.V)injection in mice usually results in systenic infecions but cannot simulate significant bradn inflammation.Here,we developed&fungal en-cephaltis model by intracerebroventriaular(L.C.V)injection af C.albicansto better simulate the significant harm und consequencEs.Compared with I.V,a greater number of colony-for ming units(CFUa)in the brain was induced following I.CV.Magnetic resonane imaging MRM resulta revesled more obvious inflammatinn in the external capsule area of the brain.Menwhile,be havioral experiments with the Y-meze also indicated that abnormal activity behavior further relected significant short-term mamory impairment after I.C.Vof C.albicans.In summary,these studies not onby provide a novel fungal encephalitis model for understanding the pathogenesis mechanism of this disease but also lay a solid foundation for future effective tretment.展开更多
BACKGROUND The oral cavity harbors more than 700 species of bacteria,which play crucial roles in the development of various oral diseases including caries,endodontic infection,periodontal infection,and diverse oral di...BACKGROUND The oral cavity harbors more than 700 species of bacteria,which play crucial roles in the development of various oral diseases including caries,endodontic infection,periodontal infection,and diverse oral diseases.AIM To investigate the antimicrobial action of Cymbopogon Schoenanthus and Pelargonium graveolens essential oils against Streptococcus mutans,Staphylococcus aureus,Candida albicans,Ca.dubliniensis,and Ca.krusei.METHODS Minimum microbicidal concentration was determined following Clinical and Laboratory Standards Institute documents.The synergistic antimicrobial activity was evaluated using the Broth microdilution checkerboard method,and the antibiofilm activity was evaluated with the 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide assay.Data were analyzed by one-way analysis of variance followed by the Tukey post-hoc test(P≤0.05).RESULTS C.schoenanthus and P.graveolens essential oils were as effective as 0.12%chlorhexidine against S.mutans and St.aureus monotypic biofilms after 24 h.After 24 h P.graveolens essential oil at 0.25%was more effective than the nystatin group,and C.schoenanthus essential oil at 0.25%was as effective as the nystatin group.CONCLUSION C.schoenanthus and P.graveolens essential oils are effective against S.mutans,St.aureus,Ca.albicans,Ca.dubliniensis,and Ca.krusei at different concentrations after 5 min and 24 h.展开更多
The following are our views regarding the“letter to the editor”(Helicobacter is preserved in yeast vacuoles!Does Koch’s postulates confirm it?)by Alipour and Gaeini,and the response“letter to the editor”(Candida ...The following are our views regarding the“letter to the editor”(Helicobacter is preserved in yeast vacuoles!Does Koch’s postulates confirm it?)by Alipour and Gaeini,and the response“letter to the editor”(Candida accommodates nonculturable Helicobacter pylori in its vacuole-Koch’s postulates aren’t applicable)by Siavoshi and Saniee.Alipour and Gaeini rejected the methods,results,discussion,and conclusions summarized in a review article by Siavoshi and Saniee.The present article reviews and discusses evidence on the evolutionary adaptation of Helicobacter pylori(H.pylori)to thrive in Candida cell vacuoles and concludes that Candida could act as a Trojan horse,transporting potentially infectious H.pylori into the stomach of humans.展开更多
Objective:Candida albicans is a common fungal pathogen that triggers complex host defense mechanisms,including coordinated innate and adaptive immune responses,to neutralize invading fungi effectively.Exploring the im...Objective:Candida albicans is a common fungal pathogen that triggers complex host defense mechanisms,including coordinated innate and adaptive immune responses,to neutralize invading fungi effectively.Exploring the immune microenvironment has the potential to inform the development of therapeutic strategies for fungal infections.Methods:The study analyzed individual immune cell profiles in peripheral blood mononuclear cells from Candida albicans-infected mice and healthy control mice using single-cell transcriptomics,fluorescence quantitative PCR,and Western blotting.We investigated intergroup differences in the dynamics of immune cell subpopulation infiltration,pathway enrichment,and differentiation during Candida albicans infection.Results:Our findings indicate that infiltration of CD4^(+)naive cells,regulatory T(Treg)cells,and Microtubules(MT)-associated cells increased after infection,along with impaired T cell activity.Notably,CD4^(+) T cells and plasma cells were enhanced after infection,suggesting that antibody production is dependent on T cells.In addition,we screened 6 hub genes,transcription factor forkhead box protein 3(Foxp3),cytotoxic T-lymphocyte associated protein 4(CTLA4),Interleukin 2 Receptor Subunit Beta(Il2rb),Cd28,C-C Motif Chemokine Ligand 5(Ccl5),and Cd27 for alterations associated with CD4^(+) T cell differentiation.Conclusions:These results provide a comprehensive immunological landscape of the mechanisms of Candida albicans infection and greatly advance our understanding of adaptive immunity in fungal infections.展开更多
The gut microbiome plays a key role in the pathogenesis and disease activity of inflammatory bowel disease(IBD).While research has focused on the bacterial microbiome,recent studies have shifted towards host genetics ...The gut microbiome plays a key role in the pathogenesis and disease activity of inflammatory bowel disease(IBD).While research has focused on the bacterial microbiome,recent studies have shifted towards host genetics and host-fungal interactions.The mycobiota is a vital component of the gastrointestinal microbial community and plays a significant role in immune regulation.Among fungi,Candida species,particularly Candida albicans(C.albicans),have been extensively studied due to their dual role as gut commensals and invasive pathogens.Recent findings indicate that various strains of C.albicans exhibit consid-erable differences in virulence factors,impacting IBD's pathophysiology.Intestinal fungal dysbiosis and antifungal mucosal immunity may be associated to IBD,especially Crohn's disease(CD).This article discusses intestinal fungal dysbiosis and antifungal immunity in healthy individuals and CD patients.It discusses factors influencing the mycobiome's role in IBD pathogenesis and highlights significant contributions from the scientific community aimed at enhancing understanding of the mycobiome and encouraging further research and targeted intervention studies on specific fungal populations.Our article also provided insights into a recent study by Wu et al in the World Journal of Gastroenterology regarding the role of the gut microbiota in the pathogenesis of CD.展开更多
Candida auris since it discovery in 2009 is becoming a severe threat to human health due to its very quickly spread, its worldwide high resistance to systemic antifungal drugs. In resource-constrained settings where s...Candida auris since it discovery in 2009 is becoming a severe threat to human health due to its very quickly spread, its worldwide high resistance to systemic antifungal drugs. In resource-constrained settings where several conditions are met for its emergence and spread, this worrisome fungus could cause large hospital and/or community-based outbreaks. This review aimed to summarize the available data on C. auris in Africa focusing on its epidemiology and antifungal resistance profile. Major databases were searched for articles on the epidemiology and antifungal resistance profile of C. auris in Africa. Out of 2,521 articles identified 22 met the inclusion criteria. In Africa, nearly 89% of African countries have no published data on C. auris. The prevalence of C. auris in Africa was 8.74%. The case fatality rate of C. auris infection in Africa was 39.46%. The main C. auris risk factors reported in Africa were cardiovascular disease, renal failure, diabetes, HIV, recent intake of antimicrobial drugs, ICU admissions, surgery, hemodialysis, parenteral nutrition and indwelling devices. Four phylogenetic clades were reported in Africa, namely clades I, II, III and IV. Candida auris showed a pan-African very high resistance rate to fluconazole, moderate resistance to amphotericin B, and high susceptibility to echinocandins. Finally, C. auris clade-specific mutations were observed within the ERG2, ERG3, ERG9, ERG11, FKS1, TAC1b and MRR1 genes in Africa. This systematic review showed the presence of C. auris in the African continent and a worrying unavailability of data on this resilient fungus in most African countries.展开更多
Samaranayake L. Commensal Oral Candida in Asian Cohorts. International Journal of Oral Science, 1 （1）： 2-5, 2009The oral carriage rate of Candida in healthy humans ranges from 40% to 60%. However for a prolonged pe...Samaranayake L. Commensal Oral Candida in Asian Cohorts. International Journal of Oral Science, 1 （1）： 2-5, 2009The oral carriage rate of Candida in healthy humans ranges from 40% to 60%. However for a prolonged period, the oral candidal prevalence in humans was documented essentially using data from studies in the West as their prevalence in inhabitants in different regions of the world,including Asia was not known. Yet, recent reports from a number of studies indicate the quality, quantity and prevalence of oral yeasts differ between Asia and other regions for reason that are still unclear. This mini review on such data from Asian studies on oral carriage of Candida provides another intriguing facet of the behavior of this ubiquitous yeast.展开更多
文摘In Candida species,the endoplasmic reticulum(ER)stress response—regulated by the unfolded protein response(UPR)—serves as a critical adaptive mechanism affecting both pathogenicity and antifungal resistance.This review aims to synthesize current knowledge on ER stress pathways in Candida glabrata and Candida albicans,highlighting their species-specific adaptations and therapeutic implications.We systematically analyzed peer-reviewed literature on ER stress mechanisms in Candida,focusing on comparative studies of UPR signaling.Emphasis was placed on C.glabrata’s inositol-requiring enzyme 1(IRE1)-dependent Regulated IRE1-Dependent Decay(RIDD)pathway and C.albicans’IRE1/HAC1 and calcium-mediated pathways.Connections to virulence and drug resistance were evaluated through genetic,transcriptomic,and phenotypic evidence.Candida species employ divergent UPR strategies:C.glabrata mitigates ER stress primarily via RIDD-mediated mRNA decay to reduce protein load,while C.albicans enhances folding capacity through HAC1 splicing and calcium homeostasis.These adaptations promote survival in hostile host environments(e.g.,oxidative stress,immune attacks)and are linked to resistance against azoles and echinocandins.Pharmacological disruption of UPR components(e.g.,IRE1 inhibitors)sensitizes Candida to antifungals in experimental models.ER stress response pathways are promising targets for antifungal drug development.Understanding species-specific UPR mechanisms in Candida could guide novel therapies to overcome resistance and improve treatment outcomes.
基金Supported by Gansu Province Joint Fund General Program,No.24JRRA878Gansu Provincial Science and Technology Program Project,No.24JRRA1020+2 种基金Gansu Province Key Talent Program,No.2025RCXM006Teaching Research and Reform Program for Postgraduate Education at Gansu University of Traditional Chinese Medicine(GUSTCM),No.YBXM-202406Special Fund for Mentors of“Qihuang Talents”in the First-Level Discipline of Chinese Medicine,No.ZYXKBD-202415。
文摘BACKGROUND Emerging evidence implicates Candida albicans(C.albicans)in human oncogenesis.Notably,studies have supported its involvement in regulating outcomes in colorectal cancer(CRC).This study investigated the paradoxical role of C.albicans in CRC,aiming to determine whether it promotes or suppresses tumor development,with a focus on the mechanistic basis linked to its metabolic profile.AIM To investigate the dual role of C.albicans in the development and progression of CRC through metabolite profiling and to establish a prognostic model that integrates the microbial and metabolic interactions in CRC,providing insights into potential therapeutic strategies and clinical outcomes.METHODSA prognostic model integrating C. albicans with CRC was developed, incorporating enrichment analysis, immuneinfiltration profiling, survival analysis, Mendelian randomization, single-cell sequencing, and spatial transcriptomics.The effects of the C. albicans metabolite mixture on CRC cells were subsequently validated in vitro. Theprimary metabolite composition was characterized using liquid chromatography-mass spectrometry.RESULTSA prognostic model based on five specific mRNA markers, EHD4, LIME1, GADD45B, TIMP1, and FDFT1, wasestablished. The C. albicans metabolite mixture significantly reduced CRC cell viability. Post-treatment analysisrevealed a significant decrease in gene expression in HT29 cells, while the expression levels of TIMP1, EHD4, andGADD45B were significantly elevated in HCT116 cells. Conversely, LIME1 expression and that of other CRC celllines showed reductions. In normal colonic epithelial cells (NCM460), GADD45B, TIMP1, and FDFT1 expressionlevels were significantly increased, while LIME1 and EHD4 levels were markedly reduced. Following metabolitetreatment, the invasive and migratory capabilities of NCM460, HT29, and HCT116 cells were reduced. Quantitativeanalysis of extracellular ATP post-treatment showed a significant elevation (P < 0.01). The C. albicans metabolitemixture had no effect on reactive oxygen species accumulation in CRC cells but led to a reduction in mitochondrialmembrane potential, increased intracellular lipid peroxidation, and induced apoptosis. Metabolomic profilingrevealed significant alterations, with 516 metabolites upregulated and 531 downregulated.CONCLUSIONThis study introduced a novel prognostic model for CRC risk assessment. The findings suggested that the C.albicans metabolite mixture exerted an inhibitory effect on CRC initiation.
文摘Background: Vulvovaginal candidiasis (VVC) is a common cause of significant morbidity, affecting millions of women worldwide. It is estimated that approximately 75%of women of childbearing age will have at least one episode of candidiasis in their lifetime. In the last decades, resistance to azoles has become a public health problem. Although studies on vulvovaginitis have been done, there is lack of VVC studies in our area. The aim of this study was to describe the etiological and resistance profiles of vulvovaginal candidiasis to standard antifungus at the Saint Camille Hospital of Ouagadougou (HOSCO), Burkina Faso. Methods: We conducted a prospective study from January 2018 to December 2022. From vulvovaginal swabs, Candida species were identified using the ChromID® Candida Agar medium and the API® Candida gallery. Antifungal susceptibility testing was performed using Kirby-Bauer agar disk diffusion. Results: A total of 4789 women were sampled. The average age of sexually active women was 27.80+/−6.77 years, with extremes ranging from 15 to 64 years. Vaginal Candida infections accounted for 74.16% of the cases. The 20 - 29 age group was the most affected by vulvovaginal candidiasis. Pregnant women accounted for 28.76% of our study population. Women in the second (2nd) trimester of pregnancy had more Candida infections. Candida albicans was the most isolated species (55.12%), followed by Candida glabrata (27.64%), Candida tropicalis (6.91%), Candida famata (6.67%), Candida krusei (2.56%). All the Candida species isolated showed very high of resistance to Fluconazole (45.2%), Miconazole (23.7%) and Clotrimazole (45.7%). Conclusion: Species-specific antifungal results should always be considered to avoid antifungal resistance associated with vulvovaginal candidiasis. Identifying the causative species using vaginal fungal cultures can help guide therapy and improve outcomes for these patients.
基金supported by the Research and Development of Ecological Control Mechanisms and Application Techniques for Forest Pest and Disease in the North and South Mountains of Lhasa(No.XZ202301ZY0019N)Construction and Comprehensive Service Cap ability Enhancement of Plateau Characteristic Agricultural and Animal Husbandry Science and Technology Small Courtyard(XK202403)。
文摘This study examined the EAG(Electroantennogram)responses of Stilpnotia candida to a mix of host plant volatiles and to provide a foundation for the development of plant-derived attractants.During the peak period of adult eclosion,gas chromatography–mass spectrometry analyzed and identified the volatiles emitted by Populus×beijingensis found in Xizang.Based on the preliminary EAG experiments and the GC‒MS results,a blending scheme was developed.EAG and Y-tube-olfactometry were employed to measure the electrophysiological and behavioural responses of unmated males and females 24 h after eclosion 10 blends of volatiles derived from five host plants.The GC–MS analysis revealed 22 volatile compounds from Populus×beijingensis leaves,composed of esters,hydrocarbons,terpenoids,alcohols,phenols,and ether.The results indicated that all 10 blending schemes produced EAG responses in mature S.candida.The concentration thresholds were between 1 and 10μg·μL^(-1),above the optimal concentration,and a corresponding decrease in EAG was observed.According to intergroup comparisons,mature S.candida had more pronounced EAG responses.Under different concentrations,there were significant differences in the EAG from male and female S.candida to each blending scheme.Behavioural response tests indicated that schemes 2,7,and 8 exhibited significantly greater attractiveness to adult S.candida.The combined results from the EAG and behavioral response experiments demonstrated that unmated male and female adult S.candida have varying degrees of sensitivity to the volatile compounds from the 10 blending schemes specific to Xizangan Populus×beijingensis.Schemes 2,7,and 8 showed robust EAG responses and attractive behavioural responses to both male and female adult S.candida.
文摘A reliable estimation of Candida bloodstream infection prevalence is increasingly important to track changes in Candida species distribution and define burden of ongoing candidemia.A systematic review and meta-analysis were conducted to estimate candidemia prevalence and identify patterns of Candida species in South-eastern Asia.Systematic electronic-databases literature search was performed on published studies recorded candidemia prevalence in South-Eastern Asia.Using meta-analysis of proportions,the overall pooled prevalences of candidemia by Candida(C.)albicans,C.tropicalis,C.parapsilosis and C.glabrata were calculated as 28.4%(95%CI 24.9-31.8),29.2%(95%CI 24.7-33.7),19.1%(95%CI 14.8-23.4)and 14.0%(95%CI 10.4-17.5),respectively.Based on publication year and country,subgroup analyses were conducted on Candida species to determine heterogeneity source.The findings may not precisely reflect true candidemia prevalence in different countries.Therefore,it highlights continuous need to conduct prevalence studies,assess and monitor growing burden,control effect of potential risk factors and implement regional surveillance programs to prevent further rise.
基金sponsored by the projects funded by the Natural Science Foundation(Key project)of University in Anhui province(grant numbers 2023AH050727,2023AH040114,2023AH052278).
文摘Background:Houttuynia cordata injection(HCI),made from fresh Houttuynia cordata,exerts heat-clearing,detoxifying and diuretic effects.It is indicated for various infections including lung abscess,fever,leucorrhea,urinary tract infection and carbuncle.Candida albicans is a common opportunistic pathogen in immunocompromised individuals.This pathogenic fungus colonizes in skin,mucosa membrane and digestive tract,potentially progressing from localized mucosal infections to systemic disease.Methods:The minimum inhibitory concentration(MIC)of HCI against C.albicans strain was determined by the microdilution method.The hyphal status of C.albicans was observed after incubation in the liquid medium and evaluated by Gram staining.The biofilm formation ability was measured using XTT reduction assay and assessed by Calcofluor White staining.The expression of virulence-related genes was detected with quantitative RT-PCR.Results:The MIC of HCI against C.albicans strain was determined to be 0.5 g/mL.At this concentration,HCI exhibited inhibitory effects on hyphal formation,as confirmed by both liquid medium observation and Gram staining.HCI at the MIC also effectively inhibited C.albicans biofilm formation,which was verified through XTT reduction assay and Calcofluor White staining.Additionally,gene expression analysis revealed that HCI significantly suppressed the expression of virulence-related genes in C.albicans.Conclusion:HCI demonstrates inhibitory effects on C.albicans growth and biofilm development.It inhibits hyphal formation,affecting the yeast-to-hyphal transition.This study investigated the antifungal effects of HCI,providing potent experimental evidence for its mechanism of action against C.albicans.
文摘Candida albicans(C.albicans)represents one of the most prevalent opportunistic fungal pathogens in cancer patients.Although the association between C.albicans and cancer has been recognized for decades,the causal relationship,whether C.albicans infection is a consequence of cancer or a direct contributor to cancer development-remains a subject of intensive investigation.Recently,the complex interplay between microbes and cancer has garnered significant attention within the scientific community,with growing interest in elucidating the underlying molecular mechanisms.This review systematically examines the biological characteristics of C.albicans,its multifaceted interactions with the host,and its relationship with the intestinal microbiota.Additionally,it provides a comprehensive analysis of the association between C.albicans and the development of various malignancies,with particular emphasis on digestive tract cancers.The review also identifies critical knowledge gaps and apparent contradictions in existing research,highlighting potential avenues for breakthroughs that will advance the efficient and accurate screening,diagnosis,and treatment of cancer.
基金financially supported by the ResearchInitiation Ftnd Project at Hainan University(Grant Nos.KYQD(ZR)20078 and KYQD(ZR)23179).
文摘Candida albicans(C.abicans),вcommon pathogenic fungus in nature,has enough cæpity to cause Severe brain infection through various mesns under immunocompromised conditions.Currently,stablishing a basic animal disesse model has become the main rsrch tool,which isconducive to simulat ing fungal encephalitis effectively.However,the widely used bloodbarne infection model established by intravenoOus(I.V)injection in mice usually results in systenic infecions but cannot simulate significant bradn inflammation.Here,we developed&fungal en-cephaltis model by intracerebroventriaular(L.C.V)injection af C.albicansto better simulate the significant harm und consequencEs.Compared with I.V,a greater number of colony-for ming units(CFUa)in the brain was induced following I.CV.Magnetic resonane imaging MRM resulta revesled more obvious inflammatinn in the external capsule area of the brain.Menwhile,be havioral experiments with the Y-meze also indicated that abnormal activity behavior further relected significant short-term mamory impairment after I.C.Vof C.albicans.In summary,these studies not onby provide a novel fungal encephalitis model for understanding the pathogenesis mechanism of this disease but also lay a solid foundation for future effective tretment.
文摘BACKGROUND The oral cavity harbors more than 700 species of bacteria,which play crucial roles in the development of various oral diseases including caries,endodontic infection,periodontal infection,and diverse oral diseases.AIM To investigate the antimicrobial action of Cymbopogon Schoenanthus and Pelargonium graveolens essential oils against Streptococcus mutans,Staphylococcus aureus,Candida albicans,Ca.dubliniensis,and Ca.krusei.METHODS Minimum microbicidal concentration was determined following Clinical and Laboratory Standards Institute documents.The synergistic antimicrobial activity was evaluated using the Broth microdilution checkerboard method,and the antibiofilm activity was evaluated with the 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide assay.Data were analyzed by one-way analysis of variance followed by the Tukey post-hoc test(P≤0.05).RESULTS C.schoenanthus and P.graveolens essential oils were as effective as 0.12%chlorhexidine against S.mutans and St.aureus monotypic biofilms after 24 h.After 24 h P.graveolens essential oil at 0.25%was more effective than the nystatin group,and C.schoenanthus essential oil at 0.25%was as effective as the nystatin group.CONCLUSION C.schoenanthus and P.graveolens essential oils are effective against S.mutans,St.aureus,Ca.albicans,Ca.dubliniensis,and Ca.krusei at different concentrations after 5 min and 24 h.
基金Supported by the National Natural Science Foundation of China,No.82260402Basic Research Program of Guizhou Science and Technology Plan,No.ZK[2022]341Foundation of Key Laboratory of Education Department of Guizhou province,No.[2022]019.
文摘The following are our views regarding the“letter to the editor”(Helicobacter is preserved in yeast vacuoles!Does Koch’s postulates confirm it?)by Alipour and Gaeini,and the response“letter to the editor”(Candida accommodates nonculturable Helicobacter pylori in its vacuole-Koch’s postulates aren’t applicable)by Siavoshi and Saniee.Alipour and Gaeini rejected the methods,results,discussion,and conclusions summarized in a review article by Siavoshi and Saniee.The present article reviews and discusses evidence on the evolutionary adaptation of Helicobacter pylori(H.pylori)to thrive in Candida cell vacuoles and concludes that Candida could act as a Trojan horse,transporting potentially infectious H.pylori into the stomach of humans.
基金supported by National Key Research and Development Program of China(2021YFC2301405)Chongqing Talent Program(No.CQYC202003220).
文摘Objective:Candida albicans is a common fungal pathogen that triggers complex host defense mechanisms,including coordinated innate and adaptive immune responses,to neutralize invading fungi effectively.Exploring the immune microenvironment has the potential to inform the development of therapeutic strategies for fungal infections.Methods:The study analyzed individual immune cell profiles in peripheral blood mononuclear cells from Candida albicans-infected mice and healthy control mice using single-cell transcriptomics,fluorescence quantitative PCR,and Western blotting.We investigated intergroup differences in the dynamics of immune cell subpopulation infiltration,pathway enrichment,and differentiation during Candida albicans infection.Results:Our findings indicate that infiltration of CD4^(+)naive cells,regulatory T(Treg)cells,and Microtubules(MT)-associated cells increased after infection,along with impaired T cell activity.Notably,CD4^(+) T cells and plasma cells were enhanced after infection,suggesting that antibody production is dependent on T cells.In addition,we screened 6 hub genes,transcription factor forkhead box protein 3(Foxp3),cytotoxic T-lymphocyte associated protein 4(CTLA4),Interleukin 2 Receptor Subunit Beta(Il2rb),Cd28,C-C Motif Chemokine Ligand 5(Ccl5),and Cd27 for alterations associated with CD4^(+) T cell differentiation.Conclusions:These results provide a comprehensive immunological landscape of the mechanisms of Candida albicans infection and greatly advance our understanding of adaptive immunity in fungal infections.
文摘The gut microbiome plays a key role in the pathogenesis and disease activity of inflammatory bowel disease(IBD).While research has focused on the bacterial microbiome,recent studies have shifted towards host genetics and host-fungal interactions.The mycobiota is a vital component of the gastrointestinal microbial community and plays a significant role in immune regulation.Among fungi,Candida species,particularly Candida albicans(C.albicans),have been extensively studied due to their dual role as gut commensals and invasive pathogens.Recent findings indicate that various strains of C.albicans exhibit consid-erable differences in virulence factors,impacting IBD's pathophysiology.Intestinal fungal dysbiosis and antifungal mucosal immunity may be associated to IBD,especially Crohn's disease(CD).This article discusses intestinal fungal dysbiosis and antifungal immunity in healthy individuals and CD patients.It discusses factors influencing the mycobiome's role in IBD pathogenesis and highlights significant contributions from the scientific community aimed at enhancing understanding of the mycobiome and encouraging further research and targeted intervention studies on specific fungal populations.Our article also provided insights into a recent study by Wu et al in the World Journal of Gastroenterology regarding the role of the gut microbiota in the pathogenesis of CD.
文摘Candida auris since it discovery in 2009 is becoming a severe threat to human health due to its very quickly spread, its worldwide high resistance to systemic antifungal drugs. In resource-constrained settings where several conditions are met for its emergence and spread, this worrisome fungus could cause large hospital and/or community-based outbreaks. This review aimed to summarize the available data on C. auris in Africa focusing on its epidemiology and antifungal resistance profile. Major databases were searched for articles on the epidemiology and antifungal resistance profile of C. auris in Africa. Out of 2,521 articles identified 22 met the inclusion criteria. In Africa, nearly 89% of African countries have no published data on C. auris. The prevalence of C. auris in Africa was 8.74%. The case fatality rate of C. auris infection in Africa was 39.46%. The main C. auris risk factors reported in Africa were cardiovascular disease, renal failure, diabetes, HIV, recent intake of antimicrobial drugs, ICU admissions, surgery, hemodialysis, parenteral nutrition and indwelling devices. Four phylogenetic clades were reported in Africa, namely clades I, II, III and IV. Candida auris showed a pan-African very high resistance rate to fluconazole, moderate resistance to amphotericin B, and high susceptibility to echinocandins. Finally, C. auris clade-specific mutations were observed within the ERG2, ERG3, ERG9, ERG11, FKS1, TAC1b and MRR1 genes in Africa. This systematic review showed the presence of C. auris in the African continent and a worrying unavailability of data on this resilient fungus in most African countries.
文摘Samaranayake L. Commensal Oral Candida in Asian Cohorts. International Journal of Oral Science, 1 （1）： 2-5, 2009The oral carriage rate of Candida in healthy humans ranges from 40% to 60%. However for a prolonged period, the oral candidal prevalence in humans was documented essentially using data from studies in the West as their prevalence in inhabitants in different regions of the world,including Asia was not known. Yet, recent reports from a number of studies indicate the quality, quantity and prevalence of oral yeasts differ between Asia and other regions for reason that are still unclear. This mini review on such data from Asian studies on oral carriage of Candida provides another intriguing facet of the behavior of this ubiquitous yeast.
