AIM: To characterize the biochemical and immunological properties of an experimental ISCOMS vaccine prepared from a novel therapeutic polypeptide based on T cell epitopes of HBsAg, and a heptatis B-ISCOMS was prepared...AIM: To characterize the biochemical and immunological properties of an experimental ISCOMS vaccine prepared from a novel therapeutic polypeptide based on T cell epitopes of HBsAg, and a heptatis B-ISCOMS was prepared and investigated. METHODS: An immunostimulating complexes(ISCOMS)-based vaccine containing a novel therapeutic hepatitis B polypeptide was prepared by dialysis method, and its formation was visualized by electron microscopy and biochemically verified by SDS-polyacrylamide gel electrophoresis. Amount of the peptide within ISCOMS was determined by Bradford assay, and specific CTL response was detected by ELISPOT assay. RESULTS: Typical cage-like structures of submicroparticle with a diameter of about 40nm were observed by electron microscopy. Results from Bradford assay showed that the level of peptide incorporation was about 0.33g.L(-1). At the paralleled position close to the sixth band of the molecular weight marker(3480kDa) a clear band was shown in SDS-PAGE analysis, indicating successful incorporation of polypeptide into ISCOMS. It is suggested that ISCOMS delivery system could efficiently improve the immunogenicity of polypeptide and elicit specific immune responses in vivo by the results of ELISPOT assay, which showed that IFN-gamma producing cells(specific CTL responses) were increased(spots of ISCOMS-treated group: 47+/-5, n =3; control group: 5+/-2, n =3). CONCLUSION: ISCOMS-based hepatitis B polypeptide vaccine is successfully constructed and it induces a higher CTL response compared with short polypeptides vaccine in vivo.展开更多
AIM: To establish clone cells with different metastatic potential for the study of metastasis-related mechanisms. METHODS: Cloning procedure was performed on parental hepatocellular carcinoma (HCC) cell line MHCC97, a...AIM: To establish clone cells with different metastatic potential for the study of metastasis-related mechanisms. METHODS: Cloning procedure was performed on parental hepatocellular carcinoma (HCC) cell line MHCC97, and biological characteristics of the target clones selected by in vivo screening were studied. RESULTS: Two clones with high (MHCC97-H) and low (MHCC97-L) metastatic potential were isolated from the parent cell line. Compared with MHCC97-L, MHCC97-H had smaller cell size (average cell diameter 43 microm vs 50 microm) and faster in vitro and in vivo growth rate (tumor cell doubling time was 34.2h vs 60.0h). The main ranges of chromosomes were 55-58 in MHCC97-H and 57-62 in MHCC97-L. Boyden chamber in vitro invasion assay demonstrated that the number of penetrating cells through the artificial basement membrane was (37.5 +/- 11.0) cells/field for MHCC97-H vs (17.7 +/- 6.3)/field for MHCC97-L. The proportions of cells in G0-G1 phase, S phase, and G2-M phase for MHCC97-H/MHCC97-L were 0.56/0.65, 0.28/0.25 and 0.16/0.10, respectively, as measured by flow cytometry. The serum AFP levels in nude mice 5wk after orthotopic implantation of tumor tissue were (246 +/- 66) microg.L(-1) for MHCC97-H and (91 +/- 66) microg.L(-1) for MHCC97-L. The pulmonary metastatic rate was 100% (10/10) vs 40% (4/10). CONCLUSION: Two clones of the same genetic background but with different biological behaviors were established, which could be valuable models for investigation on HCC metastasis.展开更多
As an emerging two-dimensional (2D) nanomaterial, single layer ora few layers of molybdenum disulfide (MoS_2) has drawn significant attention in the past decade. In the present work, we report a strategy in direct cre...As an emerging two-dimensional (2D) nanomaterial, single layer ora few layers of molybdenum disulfide (MoS_2) has drawn significant attention in the past decade. In the present work, we report a strategy in direct creating polymer brushes on MoS_2 surfaces via S—C bond in the presence of UV light. The modification of MoS_2 nanosheets can be achieved bilaterally or homolaterally by performing the UVgrafting polymerization on exfoliated MoS_2 nanosheets or a single layer of MoS_2 deposited on a silicon substrate. A series of vinyl monomers including methyl methacrylate (MMA), styrene (St) and 2-isopropenyl-2-oxazoline (IPOx) could be applied to this approach, leading to the formation of poly (methyl methacrylate) (PMMA), polystyrene (PS) and poly (2-isopropenyl-2-oxazoline) (PIPOx) brushes.AFM, IR, and XPS characterizations indicate the successful formation of homogeneous brush layers on MoS_2 surfaces. The polymer brushes modified MoS_2 may found potential applications in photo dynamic therapy and sensing technologies.展开更多
南海北部东沙海区是天然气水合物有利远景区,关于其甲烷厌氧氧化作用(AOM)的沉积记录研究相对较少,不利于探讨东沙海区甲烷渗漏与该海区气候环境演化的耦合关系。本研究通过对南海北部东沙海区D7和DSF两根沉积柱的总硫含量(TS)、硫碳比...南海北部东沙海区是天然气水合物有利远景区,关于其甲烷厌氧氧化作用(AOM)的沉积记录研究相对较少,不利于探讨东沙海区甲烷渗漏与该海区气候环境演化的耦合关系。本研究通过对南海北部东沙海区D7和DSF两根沉积柱的总硫含量(TS)、硫碳比值(S/C)、正构烷烃、甘油二烷基甘油四醚(GDGTs)类化合物的分析,重建了东沙海区自11.3 ka BP以来甲烷厌氧氧化作用的沉积记录和海水表层温度(SST)。两根沉积柱的TS、S/C值、GDGT-0、古菌醇和甲烷指数值(MI)的剖面变化表明东沙海区在6.4~7.5 ka BP和0.75~1.3 ka BP时发生了AOM作用,在4.0~4.2kaBP时可能发生了AOM作用,存在甲烷渗漏现象。由TEX;重建的SST显示该海区早全新世SST持续上升,晚全新世SST呈下降趋势,推测自11.3 ka BP以来东沙海区存在小范围的甲烷渗漏,但释放的甲烷并没有导致该海区表层水体温度显著升高。展开更多
基金the National Natural Science Foundation of China,No.39789010
文摘AIM: To characterize the biochemical and immunological properties of an experimental ISCOMS vaccine prepared from a novel therapeutic polypeptide based on T cell epitopes of HBsAg, and a heptatis B-ISCOMS was prepared and investigated. METHODS: An immunostimulating complexes(ISCOMS)-based vaccine containing a novel therapeutic hepatitis B polypeptide was prepared by dialysis method, and its formation was visualized by electron microscopy and biochemically verified by SDS-polyacrylamide gel electrophoresis. Amount of the peptide within ISCOMS was determined by Bradford assay, and specific CTL response was detected by ELISPOT assay. RESULTS: Typical cage-like structures of submicroparticle with a diameter of about 40nm were observed by electron microscopy. Results from Bradford assay showed that the level of peptide incorporation was about 0.33g.L(-1). At the paralleled position close to the sixth band of the molecular weight marker(3480kDa) a clear band was shown in SDS-PAGE analysis, indicating successful incorporation of polypeptide into ISCOMS. It is suggested that ISCOMS delivery system could efficiently improve the immunogenicity of polypeptide and elicit specific immune responses in vivo by the results of ELISPOT assay, which showed that IFN-gamma producing cells(specific CTL responses) were increased(spots of ISCOMS-treated group: 47+/-5, n =3; control group: 5+/-2, n =3). CONCLUSION: ISCOMS-based hepatitis B polypeptide vaccine is successfully constructed and it induces a higher CTL response compared with short polypeptides vaccine in vivo.
基金Supportod ty the State Key Basic Research Program Grant G1998051211 the Fund for Leading Specialty of Shanghai Metropolitan Bureau of Public Health.
文摘AIM: To establish clone cells with different metastatic potential for the study of metastasis-related mechanisms. METHODS: Cloning procedure was performed on parental hepatocellular carcinoma (HCC) cell line MHCC97, and biological characteristics of the target clones selected by in vivo screening were studied. RESULTS: Two clones with high (MHCC97-H) and low (MHCC97-L) metastatic potential were isolated from the parent cell line. Compared with MHCC97-L, MHCC97-H had smaller cell size (average cell diameter 43 microm vs 50 microm) and faster in vitro and in vivo growth rate (tumor cell doubling time was 34.2h vs 60.0h). The main ranges of chromosomes were 55-58 in MHCC97-H and 57-62 in MHCC97-L. Boyden chamber in vitro invasion assay demonstrated that the number of penetrating cells through the artificial basement membrane was (37.5 +/- 11.0) cells/field for MHCC97-H vs (17.7 +/- 6.3)/field for MHCC97-L. The proportions of cells in G0-G1 phase, S phase, and G2-M phase for MHCC97-H/MHCC97-L were 0.56/0.65, 0.28/0.25 and 0.16/0.10, respectively, as measured by flow cytometry. The serum AFP levels in nude mice 5wk after orthotopic implantation of tumor tissue were (246 +/- 66) microg.L(-1) for MHCC97-H and (91 +/- 66) microg.L(-1) for MHCC97-L. The pulmonary metastatic rate was 100% (10/10) vs 40% (4/10). CONCLUSION: Two clones of the same genetic background but with different biological behaviors were established, which could be valuable models for investigation on HCC metastasis.
基金financial support of this work from the Department of Science and Technology of Jilin Province (Nos. 20180101196JC and 20180101170JC) is greatly acknowledgedthe support of Youth Innovation Promotion Association CAS (No. 2016207)
文摘As an emerging two-dimensional (2D) nanomaterial, single layer ora few layers of molybdenum disulfide (MoS_2) has drawn significant attention in the past decade. In the present work, we report a strategy in direct creating polymer brushes on MoS_2 surfaces via S—C bond in the presence of UV light. The modification of MoS_2 nanosheets can be achieved bilaterally or homolaterally by performing the UVgrafting polymerization on exfoliated MoS_2 nanosheets or a single layer of MoS_2 deposited on a silicon substrate. A series of vinyl monomers including methyl methacrylate (MMA), styrene (St) and 2-isopropenyl-2-oxazoline (IPOx) could be applied to this approach, leading to the formation of poly (methyl methacrylate) (PMMA), polystyrene (PS) and poly (2-isopropenyl-2-oxazoline) (PIPOx) brushes.AFM, IR, and XPS characterizations indicate the successful formation of homogeneous brush layers on MoS_2 surfaces. The polymer brushes modified MoS_2 may found potential applications in photo dynamic therapy and sensing technologies.
文摘南海北部东沙海区是天然气水合物有利远景区,关于其甲烷厌氧氧化作用(AOM)的沉积记录研究相对较少,不利于探讨东沙海区甲烷渗漏与该海区气候环境演化的耦合关系。本研究通过对南海北部东沙海区D7和DSF两根沉积柱的总硫含量(TS)、硫碳比值(S/C)、正构烷烃、甘油二烷基甘油四醚(GDGTs)类化合物的分析,重建了东沙海区自11.3 ka BP以来甲烷厌氧氧化作用的沉积记录和海水表层温度(SST)。两根沉积柱的TS、S/C值、GDGT-0、古菌醇和甲烷指数值(MI)的剖面变化表明东沙海区在6.4~7.5 ka BP和0.75~1.3 ka BP时发生了AOM作用,在4.0~4.2kaBP时可能发生了AOM作用,存在甲烷渗漏现象。由TEX;重建的SST显示该海区早全新世SST持续上升,晚全新世SST呈下降趋势,推测自11.3 ka BP以来东沙海区存在小范围的甲烷渗漏,但释放的甲烷并没有导致该海区表层水体温度显著升高。
