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Injective Envelopes of a Hilbert C~*Module
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作者 许天周 蔡涛 李炳照 《Journal of Beijing Institute of Technology》 EI CAS 2001年第2期119-124,共6页
As in homology, the notion of injectivity is introduced in the category whose objects are Hilbert C * module over a C * algebra and whose morphism are bounded module operators. The definition of injective envelope... As in homology, the notion of injectivity is introduced in the category whose objects are Hilbert C * module over a C * algebra and whose morphism are bounded module operators. The definition of injective envelopes of an extension of a Hilbert C * modules over a C * algebra is introduced, and is characterized in terms of the injectivity and essence. It is shown that every Hilbert C * module has a unique (up to H isometrics) injective envelope if it exists. It is also shown that an extension of a Hilbert C * module is an injective envelope if and only if it is an injective and essential extension. Moreover, every Hilbert C * module over a W * algebra has a unique (up to H isometrics) injective envelope and the injective envelope of a Hilbert C * module H is maximal essential extension of H . 展开更多
关键词 c * algebra Hilbert c * module injective envelope
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Crystallization Modulation and Holistic Passivation Enables Efficient Two‑Terminal Perovskite/CuIn(Ga)Se_(2)Tandem Solar Cells
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作者 Cong Geng Kuanxiang Zhang +7 位作者 Changhua Wang Chung Hsien Wu Jiwen Jiang Fei Long Liyuan Han Qifeng Han Yi‑Bing Cheng Yong Peng 《Nano-Micro Letters》 SCIE EI CAS 2025年第1期205-215,共11页
Two-terminal(2-T)perovskite(PVK)/CuIn(Ga)Se_(2)(CIGS)tandem solar cells(TSCs)have been considered as an ideal tandem cell because of their best bandgap matching regarding to Shockley–Queisser(S–Q)limits.However,the ... Two-terminal(2-T)perovskite(PVK)/CuIn(Ga)Se_(2)(CIGS)tandem solar cells(TSCs)have been considered as an ideal tandem cell because of their best bandgap matching regarding to Shockley–Queisser(S–Q)limits.However,the nature of the irregular rough morphology of commercial CIGS prevents people from improving tandem device performances.In this paper,D-homoserine lactone hydrochloride is proven to improve coverage of PVK materials on irregular rough CIGS surfaces and also passivate bulk defects by modulating the growth of PVK crystals.In addition,the minority carriers near the PVK/C60 interface and the incompletely passivated trap states caused interface recombination.A surface reconstruction with 2-thiopheneethylammonium iodide and N,N-dimethylformamide assisted passivates the defect sites located at the surface and grain boundaries.Meanwhile,LiF is used to create this field effect,repelling hole carriers away from the PVK and C60 interface and thus reducing recombination.As a result,a 2-T PVK/CIGS tandem yielded a power conversion efficiency of 24.6%(0.16 cm^(2)),one of the highest results for 2-T PVK/CIGS TSCs to our knowledge.This validation underscores the potential of our methodology in achieving superior performance in PVK/CIGS tandem solar cells. 展开更多
关键词 PVK/cIGs Tscs Irregular rough surfaces modulating the growth PVK/c60 interface recombination
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Multilevel NAND Flash Memories with Superposition Modulation:A Non-Orthogonal Multi-User Communication Perspective
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作者 Zhou Xuan Ma Zheng +2 位作者 Zhou Yi Tang Xiaohu Fan Pingzhi 《China Communications》 2025年第3期132-147,共16页
In this work,we propose a comprehensive theoretical framework for the multilevel NAND(NOT AND logic)flash memory,built upon the modified Student’s t distribution where the distortion of the threshold voltage caused b... In this work,we propose a comprehensive theoretical framework for the multilevel NAND(NOT AND logic)flash memory,built upon the modified Student’s t distribution where the distortion of the threshold voltage caused by the random telegraph noise,cell-to-cell interference and data retention noise are jointly considered.Based on the superposition modulation,we build a non-orthogonal multiuser communication model where a linear mapping is conducted between the verify voltages and binary antipodal symbols.Aimed at improving the storage efficiency,we propose an unequal amplitude mapping(UAM)solution by optimizing the weighting coefficients of verify voltages to intelligently adjust the width of each state.Moreover,the uniform storage efficiency region and sum storage efficiency of different labelings with various decoding schemes are discussed.Simulation results validate the effectiveness of our proposed UAM solution where an up to 20.9%storage efficiency gain can be achieved compared to the current used benchmark scheme.In addition,analytical and simulation results also demonstrate that the successive cancellation decoding outperforms other decoding schemes for all labelings. 展开更多
关键词 binary labelings flash memory modified student’s t-based model superposition modulation unequal amplitude mapping
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Gorenstein Homological Dimensions and Auslander Categories with Respect to a Semidualizing Module 被引量:2
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作者 Chunxia ZHANG Limin WANG Zhongkui LIU 《Journal of Mathematical Research with Applications》 CSCD 2013年第3期297-311,共15页
Let R be a commutative noetherian local ring. In this paper, we study Gorenstein projective, injective and flat modules with respect to a semidualizing R-module C, and we give some connections between C-Gorenstein hom... Let R be a commutative noetherian local ring. In this paper, we study Gorenstein projective, injective and flat modules with respect to a semidualizing R-module C, and we give some connections between C-Gorenstein homological dimensions and the Auslander categories of R. 展开更多
关键词 semidualizing modules c-Gorenstein injective modules c-corenstein projective modules c-Gorenstein fiat modules Auslander categories.
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CHAIN CONDITIONS FOR C*-ALGEBRAS COMING FROM HILBERT C*-MODULES 被引量:2
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作者 Mahmood POURGHOLAMHOSSEIN Mohammad ROUZBEHANI Massoud AMINI 《Acta Mathematica Scientia》 SCIE CSCD 2018年第4期1163-1173,共11页
In this paper we define and study chain conditions for Hilbert C*-modules through their C*-algebras of compact operators and discuss their perseverance under Morita equivalence and tensor products. We show that thes... In this paper we define and study chain conditions for Hilbert C*-modules through their C*-algebras of compact operators and discuss their perseverance under Morita equivalence and tensor products. We show that these chain conditions are passed from the C*-algebra to its Hilbert module under certain conditions. We also study chain conditions for Hilbert modules coming from inclusion of C*-algebra with a faithful conditional expectation. 展开更多
关键词 Hilbert c*-module Noetherian and Artinian c*-algebras purely infinite c*-algebras Morita equivalence
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STABILITY OF GENERALIZED DERIVATIONS ON HILBERT C*-MODULES ASSOCIATED WITH A PEXIDERIZED CAUCHY-JENSEN TYPE FUNCTIONAL EQUATION 被引量:2
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作者 Ali Ebadian Ismail Nikoufar +1 位作者 Themistocles M.Rassias Norouz Ghobadipour 《Acta Mathematica Scientia》 SCIE CSCD 2012年第3期1226-1238,共13页
In this article, we introduce the notion of generalized derivations on Hilbert C*-modules. We use a fixed-point method to prove the generalized Hyers-Ulam-Rassias stability associated to the Pexiderized Cauchy-Jensen... In this article, we introduce the notion of generalized derivations on Hilbert C*-modules. We use a fixed-point method to prove the generalized Hyers-Ulam-Rassias stability associated to the Pexiderized Cauchy-Jensen type functional equationrf(x+y/r)+sg(x-y/s)=2h(x)for r, s ∈ R / {0} on Hilbert C*-modules, where f, g, and h are mappings from a Hilbert C*-module M to M. 展开更多
关键词 Hyers-Ulam-Rassias stability Hilbert c*-module generalized derivation fixed point
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A New Characterization on g-frames in Hilbert C^*-Modules 被引量:1
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作者 Xiang Zhong-qi 《Communications in Mathematical Research》 CSCD 2017年第2期129-134,共6页
In this note, we establish a new characterization on g-frames in Hilbert C;-modules from the operator-theoretic point of view, with which we provide a correction to one result recently obtained by Yao(Yao X Y. Some pr... In this note, we establish a new characterization on g-frames in Hilbert C;-modules from the operator-theoretic point of view, with which we provide a correction to one result recently obtained by Yao(Yao X Y. Some properties of g-frames in Hilbert C;-modules(in Chinese). Acta Math. Sinica, 2011, 54(1): 1–8.). 展开更多
关键词 Hilbert c*-module G-FRAME characterization
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<i>S</i>-Injective Modules and Rings 被引量:1
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作者 Nasr A. Zeyada 《Advances in Pure Mathematics》 2014年第1期25-33,共9页
We introduce and investigate the concept of s-injective modules and strongly s-injective modules. New characterizations of SI-rings, GV-rings and pseudo-Frobenius rings are given in terms of s-injectivity of their mod... We introduce and investigate the concept of s-injective modules and strongly s-injective modules. New characterizations of SI-rings, GV-rings and pseudo-Frobenius rings are given in terms of s-injectivity of their modules. 展开更多
关键词 s-Injective module Kasch RING Pseudo FROBENIUs RING
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Characterization of V-modules by Relative Quasi-continuity
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作者 刘仲奎 《Northeastern Mathematical Journal》 CSCD 2001年第2期151-158,共8页
We characterize V modules by relative (quasi )continuity of left R modules in this paper. It is shown that a left R module M is a V module if and only if every finitely cogenerated left R ... We characterize V modules by relative (quasi )continuity of left R modules in this paper. It is shown that a left R module M is a V module if and only if every finitely cogenerated left R module in σ[M] is S ( quasi )continuous if and only if every left R module in σ[M] is S continuous, where S is the set of all simple left R modules. We also show that a left R module M is a locally noetherian V module if and only if every semisimple left R module (in σ[M]) is M injective if and only if every essential extension in σ[M] of every semisimple left R module in σ[M] is S ? 2 (quasi )continuous, where S ? 2 is the set of all semisimple left R modules. 展开更多
关键词 V module s continuous module s quasi continuous module
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Photovoltaic Cells and Modules towards Terawatt Era 被引量:1
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作者 Vitezslav Benda 《Journal of Electronic Science and Technology》 CAS CSCD 2017年第4期351-357,共7页
Progresses in photovoltaic technologies over the past years are evident from the lower costs, the rising efficiency, to the great improvements in system reliability and yield. Cumulative installed power yearly growths... Progresses in photovoltaic technologies over the past years are evident from the lower costs, the rising efficiency, to the great improvements in system reliability and yield. Cumulative installed power yearly growths were on an average more than 40% in the period from 2007 to 2016 and in 2016, the global cumulative photovoltaic power installed has reached 320 GWp. The level 0.5 TWp could be reached before 2020. The production processes in the solar industry still have great potential for optimization both wafer based and thin film technologies. Trends following from the present technology levels are discussed, also taking into account other parts of photovoltaic systems that influence the cost of electrical energy produced. Present developments in the three generations of photovoltaic modules are discussed along with the criteria for the selection of appropriate photovoltaic module manufacturing technologies. The wafer based crystalline silicon(csilicon) technologies have the role of workhorse of present photovoltaic power generation, representing more than 90% of total module production. Further technology improvements have to be implemented without significantly increasing costs per unit, despite the necessarily more complex manufacturing processes involved. The tandem of c-silicon and thin film cells is very promising. Durability may be a limiting factor of this technology due to the dependence of the produced electricity cost on the module service time. 展开更多
关键词 crystalline silicon(c-silicon) cells photovoltaic cells photovoltaic(PV) tandem cells thin film modules
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Modular three-component radical fluoroalkyl-sulfuration of unactivated alkenes
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作者 Gao-feng Yang Zhi Liu +4 位作者 Kai Liu Xiaopeng Wu Chengjian Zhu Weipeng Li Jin Xie 《Chinese Journal of Catalysis》 2025年第2期249-258,共10页
The accompanied forge of C(sp^(3))–S and C(sp^(3))–C(sp^(3))bonds across alkene frameworks serves as a potent strategy to construct biologically important compounds.Here,we report a metal-free,photochemically mediat... The accompanied forge of C(sp^(3))–S and C(sp^(3))–C(sp^(3))bonds across alkene frameworks serves as a potent strategy to construct biologically important compounds.Here,we report a metal-free,photochemically mediated fluoroalkyl-mono/disulfuration of unactivated alkenes with high efficiency and high selectivity.A wide range of terminal and internal alkenes are good coupling partners,affording the expected products in moderate to good yields(>90 examples).The exceedingly mild reaction conditions,exceptional functional group tolerance,broad substrate scope,and the potential for late-stage modifications of pharmaceutical molecules highlight the utility of this method in the preparation of privileged motifs from readily available disulfides,tetrasulfides,and diselenides.Mechanistic studies suggest that a secondary alkyl radical intermediate undergoes efficient homolytic substitution with disulfides,facilitating the modular synthesis of a diverse array of unsymmetrical thioethers. 展开更多
关键词 c(sp^(3))-s bond Unactivated alkenes Photoredox catalysis FLUOROALKYLATION Unsymmetrical thioethers
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The characterization of Γ-modules in terms of fuzzy soft Γ-submodules 被引量:1
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作者 ZHOU Min XIANG Da-jing ZHAN Jian-ming 《Applied Mathematics(A Journal of Chinese Universities)》 SCIE CSCD 2013年第2期217-239,共23页
In this paper,we focus on combining the theories of fuzzy soft sets with Γ-modules,and establishing a new framework for fuzzy soft Γ-submodules.The main contributions of the paper are 3-fold.First,we present the con... In this paper,we focus on combining the theories of fuzzy soft sets with Γ-modules,and establishing a new framework for fuzzy soft Γ-submodules.The main contributions of the paper are 3-fold.First,we present the concepts of(R,S)-bi-Γ-submodules,quasi-Γ-submodules and regular Γ-modules.Meanwhile,some illustrative examples are given to show the rationality of the definitions introduced in this paper.Second,several new kinds of generalized fuzzy soft Γ-submodules are proposed,and related properties and mutual relationships are also investigated.Third,we discover some intrinsic connections between the generalized fuzzy soft Γ-submodules presented in this paper and crisp Γ-submodules,and describe the relationships between regular Γ-modules and the generalized fuzzy soft Γ-submodules presented in this paper. 展开更多
关键词 Fuzzy soft set Γ-module (∈ γ γ q δ)(resp. (■ γ γ δ))-fuzzy soft left R-Γ-submodule(right R-Γ-submodules (R s)-bi-Γ-submodule quasi-Γ-submodule regular Γ-module
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Linear 6通道逻辑/SPI/I^2CμModule隔离器
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《电子产品世界》 2017年第4期80-80,共1页
近日,凌力尔特公司(Linear Technology Corporation)推出6通道SPI/数字或I^2CμModule隔离器LTM2887,该器件面向低电压组件,包括较新的DSP和微处理器。两个经过良好稳压的可调电源轨(高达5V)越过隔离势垒提供大于100mA的负载电... 近日,凌力尔特公司(Linear Technology Corporation)推出6通道SPI/数字或I^2CμModule隔离器LTM2887,该器件面向低电压组件,包括较新的DSP和微处理器。两个经过良好稳压的可调电源轨(高达5V)越过隔离势垒提供大于100mA的负载电流,并具有高达62%的效率。 展开更多
关键词 module I^2c 隔离器 sPI 通道 逻辑 微处理器 负载电流
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Quasi-Modular Preserving Rank One Maps on Hilbert C~*-Modules
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作者 Bin MENG 《Journal of Mathematical Research with Applications》 CSCD 2012年第4期459-468,共10页
In this paper,we characterize a class of quasi-modular maps on Hilbert C~*-modules which map a "rank one" adjointable operator to another rank one operator.
关键词 Hilbert c^*-module coordinate inverse quasi-modular map.
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高碳含硫非调质连杆用钢C70S6精炼渣系热力学计算 被引量:1
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作者 姜晓东 杨乃辉 +5 位作者 张伟 李明林 武永孝 高旭东 刘治权 铁占鹏 《钢铁》 北大核心 2025年第7期251-261,共11页
非调质易切削钢广泛用于汽车等交通运输行业,C70S6钢是发动机胀断连杆用最具代表性的非调质易切削钢,开发并优化C70S6钢精炼造渣工艺来保障其产品成分与组织性能稳定可控,具有重要的工业价值和学术意义。钢包精炼渣系是钢包冶金的重要内... 非调质易切削钢广泛用于汽车等交通运输行业,C70S6钢是发动机胀断连杆用最具代表性的非调质易切削钢,开发并优化C70S6钢精炼造渣工艺来保障其产品成分与组织性能稳定可控,具有重要的工业价值和学术意义。钢包精炼渣系是钢包冶金的重要内容,其具有脱氧、脱硫、吸收夹杂物和稳定钢水中氮、硫含量的作用。针对C70S6钢“高碳、高硫、高氮、低铝、低硅”“三高二低”的成分控制目标与特点,基于FactSage热力学软件、KTH硫容量模型(Kth-order sufur capacity model)研究了不同精炼渣成分的特点,提出了满足硫含量控制要求的“变渣”操作工艺,即在LF(ladle furnace)精炼前期造高碱度渣强化扩散脱氧,以弥补其低铝、低硅要求造成的出钢沉淀脱氧能力不足,并为后期增氮创造了良好条件;而在精炼后期VD(vacuum degassing)阶段造低碱度渣增硫,以满足其因切削性要求保硫的目的。研究表明,钙铝比和碱度是精炼渣熔化性能的主要影响因素。C70S6钢渣系在高效脱氧脱硫阶段的目标是控制渣系碱度为3~6、MgO质量分数为4%~6%、钙铝比为1.5~2.2,渣系熔点低、流动性好,且对铝脱氧产生的Al_(2)O_(3)类夹杂物有较好的吸收和改性效果;而在保硫阶段的目标是渣系控制渣系碱度为2.0~3.5、MgO质量分数为4%~6%、钙铝比为2.3~3.3,具有较好的对钢水增硫保硫性能。此研究结果对高碳含硫非调质钢在不同阶段冶炼过程中调整渣系组元以达到钢水化学成分和洁净度的控制具有指导意义。 展开更多
关键词 c70s6钢 胀断连杆 精炼 渣系 高碱度脱氧 低碱度保硫 易切削钢 钢包
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基于PC SDK的智能装配工作站远程监控系统研究与设计 被引量:1
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作者 梁盈富 赵豆 祝战科 《机械制造与自动化》 2025年第1期131-134,共4页
以智能装配工作站为研究对象,运用机器人控制器的PC SDK接口对工作站系统进行二次开发,研究了智能装配工作站的网络架构,并在C/S架构下设计工业机器人智能装配工作站远程监控方案,开发了智能装配工作站远程监控系统。实验结果表明:该系... 以智能装配工作站为研究对象,运用机器人控制器的PC SDK接口对工作站系统进行二次开发,研究了智能装配工作站的网络架构,并在C/S架构下设计工业机器人智能装配工作站远程监控方案,开发了智能装配工作站远程监控系统。实验结果表明:该系统能够有效完成对工作站运行状态的远程监控,实现对机器人远程操作及运行状态的获取。 展开更多
关键词 Pc sDK c/s架构 远程监控 智能装配工作站
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不同NIHSS评分缺血性脑卒中患者血清CatS VILIP-1 CysC水平变化与疾病转归的相关性 被引量:1
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作者 刘义锋 余洋 +3 位作者 刘少哲 张东焕 孙孟 温昌明 《中国实用神经疾病杂志》 2025年第2期223-227,共5页
目的探讨不同NIHSS评分缺血性脑卒中(CIS)患者血清组织蛋白酶S(CatS)、视锥蛋白样蛋白-1(VILIP-1)、胱抑素C(CysC)水平变化及其与疾病转归的相关性。方法选取2021-01—2023-01南阳市中心医院收治的143例CIS患者为研究对象,根据入院时NI... 目的探讨不同NIHSS评分缺血性脑卒中(CIS)患者血清组织蛋白酶S(CatS)、视锥蛋白样蛋白-1(VILIP-1)、胱抑素C(CysC)水平变化及其与疾病转归的相关性。方法选取2021-01—2023-01南阳市中心医院收治的143例CIS患者为研究对象,根据入院时NIHSS评分分为低分组(43例,NIHSS评分<4分)、中分组(68例,NIHSS评分4~15分)和高分组(32例,NIHSS评分>15分)。比较3组入院时血清CatS、VILIP-1、CysC水平,分析其与NIHSS评分的相关性,比较不同疾病转归患者血清各指标水平,偏回归分析CIS患者转归不良的影响因素,ROC分析各指标联合检测对CIS患者转归不良的预测价值。结果入院时低分组血清CatS、VILIP-1、CysC水平分别为(53.18±6.74)ng/L、(4.57±1.38)μg/L、(1.08±0.32)mg/L,低于中分组[(76.53±9.25)ng/L、(9.63±2.44)μg/L、(1.74±0.39)mg/L]和高分组[(98.44±8.61)ng/L、(16.72±3.55)μg/L、(2.53±0.47)mg/L],各指标水平与NIHSS评分均呈正相关(P<0.05)。治疗1周、2周后转归不良患者血清CatS、VILIP-1、CysC水平[治疗1周后:(73.35±8.44)ng/L、(9.25±2.44)μg/L、(1.73±0.33)mg/L;治疗2周后:(70.96±9.02)ng/L、(8.74±2.13)μg/L、(1.71±0.30)mg/L]高于转归良好患者[治疗1周后:(55.87±6.29)ng/L、(6.15±2.03)μg/L、(1.01±0.31)mg/L;治疗2周后:(31.95±6.24)ng/L、(2.86±0.74)μg/L、(0.64±0.19)mg/L](P<0.05)。治疗1周、治疗2周后血清CatS、VILIP-1、CysC水平为CIS患者转归不良的危险因素,联合检测预测CIS患者转归不良的AUC分别为0.784、0.838。结论血清CatS、VILIP-1、CysC水平与CIS患者神经功能损伤程度及疾病转归密切相关,联合检测可作为临床判断神经功能损伤程度、预测转归情况的有效依据。 展开更多
关键词 缺血性脑卒中 疾病转归 组织蛋白酶s 视锥蛋白样蛋白-1 胱抑素c 血清
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C.S.刘易斯的莎士比亚评论及其批评史意义
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作者 辛雅敏 《山东师范大学学报(社会科学版)》 北大核心 2025年第6期34-43,共10页
作为文学批评家的C.S.刘易斯曾在20世纪30年代末40年代初公开发表过两篇莎士比亚评论文章,其中一篇反驳了当时依然流行的人物性格分析批评,另一篇则总结了莎士比亚乃至整个伊丽莎白时代戏剧语言艺术的特点,即文体上的“变奏”方法。如... 作为文学批评家的C.S.刘易斯曾在20世纪30年代末40年代初公开发表过两篇莎士比亚评论文章,其中一篇反驳了当时依然流行的人物性格分析批评,另一篇则总结了莎士比亚乃至整个伊丽莎白时代戏剧语言艺术的特点,即文体上的“变奏”方法。如果将其置于更宏大的批评史视野,刘易斯的两篇莎士比亚评论论文不仅反映了20世纪上半叶莎评风向的改变,而且对正在发生的批评观念变革起到了一定程度的推动作用。一方面,刘易斯的观点暗合了正在兴起的剑桥派批评和新批评等形式主义批评,因此也汇入了时代文学观念变革的洪流;而另一方面,他对阅读体验的重视又让其游离于主流的形式主义批评之外。但无论如何,刘易斯的莎士比亚评论都应该被视为英国文学批评史的有机组成部分。 展开更多
关键词 c.s.刘易斯 莎士比亚 文学批评
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温度对Hastelloy C-276镍基合金离子碳氮共渗组织与性能影响
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作者 曹驰 张春华 +2 位作者 陈志林 张翔 张果林 《材料科学与工艺》 北大核心 2025年第2期27-34,共8页
探究温度对镍基合金Hastelloy C-276渗层显微组织、力学性能、摩擦磨损性能以及耐蚀性能的影响规律,以期获得硬度、耐磨性和耐蚀性优良的表面改性层。使用等离子碳氮共渗技术在不同温度(395、425、455、485℃)下制备表面改性层,镍基合金... 探究温度对镍基合金Hastelloy C-276渗层显微组织、力学性能、摩擦磨损性能以及耐蚀性能的影响规律,以期获得硬度、耐磨性和耐蚀性优良的表面改性层。使用等离子碳氮共渗技术在不同温度(395、425、455、485℃)下制备表面改性层,镍基合金C-276经离子碳氮共渗后,渗层厚度从6.5到20.0μm不等,表面硬度较原始合金均有不同程度提高。当处理温度≤395℃时生成的S相层较薄;随着处理温度升高,生成较厚的S相层和CrN硬质相,获得优良的耐磨性;进一步提升处理温度至485℃时,基体表面出现贫Cr,致使合金耐蚀性降低;当处理温度<450℃时样品表现出比基体更好的耐蚀性能。镍基合金Hastelloy C-276在425、455℃温度下进行碳氮共渗,可获得硬度、耐磨性和耐蚀性相对优良的表面改性层。 展开更多
关键词 碳氮共渗 Hastelloy c-276镍基合金 s 温度 耐磨性
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血小板表面C1s蛋白表达检测方法的建立及初步应用
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作者 叶军 卢慧敏 +5 位作者 朱剑锋 花慧莲 许新 杨义力 孟超 沙敏 《临床检验杂志》 2025年第11期830-835,共6页
目的建立血小板表面C1s蛋白的流式细胞术检测方法,并初步探讨其在原发免疫性血小板减少症(ITP)辅助诊断中的潜在应用价值。方法以C1s蛋白偶联2μm羧基化磁珠制备C1s磁珠,并将C1s磁珠作为质控品,采用(FITC)标记的C1s抗体作为检测抗体,建... 目的建立血小板表面C1s蛋白的流式细胞术检测方法,并初步探讨其在原发免疫性血小板减少症(ITP)辅助诊断中的潜在应用价值。方法以C1s蛋白偶联2μm羧基化磁珠制备C1s磁珠,并将C1s磁珠作为质控品,采用(FITC)标记的C1s抗体作为检测抗体,建立血小板表面C1s蛋白表达的流式细胞术检测方法,并评价该方法的分析内、分析间精密度和线性;比较ITP组、非ITP血小板减少组与健康对照组血小板表面C1s蛋白表达水平。结果光镜观察显示,未偶联C1s蛋白的羧基化磁珠(空白磁珠)与C1s磁珠两者分散均一,无聚团现象。荧光显微镜下可见C1s磁珠呈现明显黄绿色荧光,而空白磁珠无荧光信号。所建立的流式检测方法精密度良好,低、中、高浓度C1s磁珠的分析内变异系数(CV)值分别为7.02%、7.12%、3.91%;分析间CV值分别为13.49%、6.15%、0.78%。线性良好,决定系数(R2)=0.9988。临床样本血小板表面C1s检测结果显示,ITP组C1s阳性血小板的比例(2.56±0.79)%显著高于健康对照组(0.23±0.18)%和非ITP血小板减少疾病对照组(0.22±0.10)%,差异均有统计学意义(P均<0.05)。结论成功建立了一种稳定可靠的血小板表面C1s蛋白流式细胞术检测方法,初步证实ITP患者血小板表面C1s表达显著升高,提示其可能作为ITP潜在的辅助诊断标志物。 展开更多
关键词 补体c1s 磁珠 血小板 原发免疫性血小板减少症 流式细胞术
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