This review focused on rare earth upconversion nanophosphors (UCNPs), a particular class of emitters whose photoluminescence mechanism is of fundamental difference from that of conventional dyes and semiconductor quan...This review focused on rare earth upconversion nanophosphors (UCNPs), a particular class of emitters whose photoluminescence mechanism is of fundamental difference from that of conventional dyes and semiconductor quantum dots. We in the first section gave a brief summary on a variety of synthetic methodologies developed during the past decades. Instead of presenting an exhaustive reference list, we selected only a few representative examples, illustrating the merits and limits of each involved synthetic route. Then we surveyed the recent progress in the functionalization techniques for these nanomaterials, depicting the modification in microstructures and improvement in properties with respect to the parent nanoparticles. And finally, we emphasized their application in the research fields of biolabeling and energy transfer, narrating their superior performance benefiting from the unique excitation and emission properties.展开更多
[Objectives]The therapeutic potential and action mechanism of Folium Pyrrosiae were analyzed based on the biolabel pattern.[Methods]The chemical components of Folium Pyrrosiae were analyzed by liquid chromatography-ma...[Objectives]The therapeutic potential and action mechanism of Folium Pyrrosiae were analyzed based on the biolabel pattern.[Methods]The chemical components of Folium Pyrrosiae were analyzed by liquid chromatography-mass spectrometry(LC-MS).Ten databases,including Pubchem,CTD,BindingDB,HERB,TCMIP,ETCM,SwissTargetPrediction,SuperPred webserver,TargetNet and SEA,were used in turn to retrieve the targets of related components,and key components were obtained according to the enrichment degree of targets.The obtained targets were imported into the STRING database to obtain PPI information and screen out core targets.The DAVID database was employed to analyze KEGG pathways of core targets and obtain key pathways.A key component-core target-key pathway network of Folium Pyrrosiae was constructed by Cytoscape3.10.1 software.The obtained KEGG pathways were input into the CTD database to predict corresponding diseases,and discussion and analysis were carried out.[Results]Ten key components,30 potential targets and 10 key pathways were screened out,and they participated in many diseases,of which five diseases were mainly analyzed.[Conclusions]Folium Pyrrosiae had the characteristic of multi-component,multi-target and multi-pathway synergistic effect in the treatment of lung cancer,type 2 diabetes,atherosclerosis,liver cancer,prostate cancer and other diseases,and the therapeutic potential and action mechanism of Folium Pyrrosiae were analyzed through the biolabel pattern.This study provides a research basis for further developing new functions of Folium Pyrrosiae.展开更多
Recent advancements in single-molecule biophysics have been driven by breakthroughs in advanced fluorescence microscopy techniques and the development of nextgeneration organic fluorophores.These cutting-edge fluoroph...Recent advancements in single-molecule biophysics have been driven by breakthroughs in advanced fluorescence microscopy techniques and the development of nextgeneration organic fluorophores.These cutting-edge fluorophores,coupled through tailored biolabeling strategies,offer single-molecule brightness,photostability,and phototunability(i.e.,photoswitchable,photoactivatable),contributing to enhancing spatial and temporal imaging resolution for studying biomolecular interactions and dynamics at single-event precision.This review examines the progress made over the past decade in the development of next-generation fluorophores,along with their site-specific labeling methods for proteins,nucleic acids,and biomolecular complexes.It also explores their applications in single-molecule fluorescencebased dynamic structural biology and super-resolution microscopy imaging.Furthermore,it examines ongoing efforts to address challenges associated with fluorophore photostability,photobleaching,and the integration of advanced photophysical and photochemical functionalities.The integration of state-of-the-art fluorophores with advanced labeling strategies aim to deliver complementary correlative data,holding promise for revolutionizing single-molecule biophysics by pushing the boundaries of temporal and spatial imaging resolution to unprecedented limits.展开更多
基金Project supported by the National Natural Science Foundation of China (20821091, 20971005, 20731160001, and 20931160429)Ministry of Science and Technology of China (2006CB601104)
文摘This review focused on rare earth upconversion nanophosphors (UCNPs), a particular class of emitters whose photoluminescence mechanism is of fundamental difference from that of conventional dyes and semiconductor quantum dots. We in the first section gave a brief summary on a variety of synthetic methodologies developed during the past decades. Instead of presenting an exhaustive reference list, we selected only a few representative examples, illustrating the merits and limits of each involved synthetic route. Then we surveyed the recent progress in the functionalization techniques for these nanomaterials, depicting the modification in microstructures and improvement in properties with respect to the parent nanoparticles. And finally, we emphasized their application in the research fields of biolabeling and energy transfer, narrating their superior performance benefiting from the unique excitation and emission properties.
基金Supported by National Natural Science Foundation of China(82160773).
文摘[Objectives]The therapeutic potential and action mechanism of Folium Pyrrosiae were analyzed based on the biolabel pattern.[Methods]The chemical components of Folium Pyrrosiae were analyzed by liquid chromatography-mass spectrometry(LC-MS).Ten databases,including Pubchem,CTD,BindingDB,HERB,TCMIP,ETCM,SwissTargetPrediction,SuperPred webserver,TargetNet and SEA,were used in turn to retrieve the targets of related components,and key components were obtained according to the enrichment degree of targets.The obtained targets were imported into the STRING database to obtain PPI information and screen out core targets.The DAVID database was employed to analyze KEGG pathways of core targets and obtain key pathways.A key component-core target-key pathway network of Folium Pyrrosiae was constructed by Cytoscape3.10.1 software.The obtained KEGG pathways were input into the CTD database to predict corresponding diseases,and discussion and analysis were carried out.[Results]Ten key components,30 potential targets and 10 key pathways were screened out,and they participated in many diseases,of which five diseases were mainly analyzed.[Conclusions]Folium Pyrrosiae had the characteristic of multi-component,multi-target and multi-pathway synergistic effect in the treatment of lung cancer,type 2 diabetes,atherosclerosis,liver cancer,prostate cancer and other diseases,and the therapeutic potential and action mechanism of Folium Pyrrosiae were analyzed through the biolabel pattern.This study provides a research basis for further developing new functions of Folium Pyrrosiae.
基金financial support from the National Natural Science Foundation of China(No.22374075)the Start-up Research Fund of Southeast University(4031002412).
文摘Recent advancements in single-molecule biophysics have been driven by breakthroughs in advanced fluorescence microscopy techniques and the development of nextgeneration organic fluorophores.These cutting-edge fluorophores,coupled through tailored biolabeling strategies,offer single-molecule brightness,photostability,and phototunability(i.e.,photoswitchable,photoactivatable),contributing to enhancing spatial and temporal imaging resolution for studying biomolecular interactions and dynamics at single-event precision.This review examines the progress made over the past decade in the development of next-generation fluorophores,along with their site-specific labeling methods for proteins,nucleic acids,and biomolecular complexes.It also explores their applications in single-molecule fluorescencebased dynamic structural biology and super-resolution microscopy imaging.Furthermore,it examines ongoing efforts to address challenges associated with fluorophore photostability,photobleaching,and the integration of advanced photophysical and photochemical functionalities.The integration of state-of-the-art fluorophores with advanced labeling strategies aim to deliver complementary correlative data,holding promise for revolutionizing single-molecule biophysics by pushing the boundaries of temporal and spatial imaging resolution to unprecedented limits.
