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Applying Fuzzy Matter-Element Model Based on AHP to Evaluating Bids of Water Saving Irrigation Project 被引量:4
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作者 PANFeng FUQiang +1 位作者 LIANGChuan LIUDong 《Journal of Northeast Agricultural University(English Edition)》 CAS 2003年第2期147-151,共5页
Owing to overcoming the characteristics that there are many economic and technical indexes which are fuzzy and incompatibility to each other in evaluating investment project,a new method is proposed.The method is base... Owing to overcoming the characteristics that there are many economic and technical indexes which are fuzzy and incompatibility to each other in evaluating investment project,a new method is proposed.The method is based on the matter-element analysis and combined with the concepts of fuzzy mathematics,which is called the method of fuzzy matter-element analysis.It constructs the compound fuzzy matter element with the investment projects,evaluation factors and their fuzzy value.Through establishing the best subjection degree (fuzzy value),complex fuzzy matter element of relational coefficient and weight aggregation of fuzzy matter-element model,the writer achieves on optimum order of the investment projects according to the calculated relational degree and finds the best project.In this paper,the calculation of weight adopts the analytical hierarchy process method(AHP).Through the actual example,it shows that the model is simple and its calculation is reliable.It is very significant for the engineering evaluated bid and investment decision. 展开更多
关键词 fuzzy matter element water saving irrigation evaluating bid AHP relational degree
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Detection Tool for Unbalanced Bids
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作者 Babak Nikpour Ahmed Senouci Neil Eldin 《Open Journal of Civil Engineering》 2017年第3期409-422,共14页
Unbalanced bidding is a cash flow management strategy that is recognized as an illegal/disqualifying practice by public owners;and unethical practice by most private owners. This practice provides the awarded bidder w... Unbalanced bidding is a cash flow management strategy that is recognized as an illegal/disqualifying practice by public owners;and unethical practice by most private owners. This practice provides the awarded bidder with unjustified advantages at the expense of the owner. Unfortunately, limited tools and techniques are currently available to identify and detect unbalanced bids during the evaluation process. This paper presents an innovative detection tool to identify unbalanced bids in unit price contracts during the bid evaluation process. The proposed technique develops BMDI graphs to visualize total markup variation patterns during the project lifetime to detect unbalanced bids. The proposed method also uses Monte Carlo simulation to take in consideration the impact of cost uncertainties and risks. An illustrative example was presented to show the capabilities and features of the proposed method in determining the status of submitted bids during the evaluation process. 展开更多
关键词 Unbalanced BIDDING DETECTION BID Evaluation Construction PROJECTS Mon-te-Carlo Simulation
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Reinforcement Learning Based Bidding Method with High-dimensional Bids in Electricity Markets
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作者 Jinyu Liu Hongye Guo +4 位作者 Yun Li Qinghu Tang Fuquan Huang Tunan Chen Haiwang Zhong 《Journal of Modern Power Systems and Clean Energy》 2025年第4期1373-1382,共10页
Over the past decade,bidding in electricity mar kets has attracted widespread attention.Reinforcement learning(RL)has been widely used for electricity market bidding as a powerful artificial intelligence(AI)tool to ma... Over the past decade,bidding in electricity mar kets has attracted widespread attention.Reinforcement learning(RL)has been widely used for electricity market bidding as a powerful artificial intelligence(AI)tool to make decisions under real-world uncertainties.However,current RL-based bidding methods mostly employ low-dimensional bids(LDBs),which sig nificantly diverge from the N price-power pairs commonly used in current electricity markets.The N-pair bid format is denoted as high-dimensional bid(HDB)format,which has not been ful ly integrated into the existing RL-based bidding methods.The loss of flexibility of current RL-based bidding methods could greatly limit the bidding profits and make it difficult to address the increasing uncertainties caused by renewable energy genera tion.In this paper,we propose a framework for fully utilizing HDBs in RL-based bidding methods.First,we employ a special type of neural network called the neural network supply func tion(NNSF)to generate HDBs in the form of N price-power pairs.Second,we embed the NNSF into a Markov decision pro cess(MDP)to make it compatible with most existing RL algo rithms.Finally,the experiments on energy storage systems(ES Ss)in the Pennsylvania-New Jersey-Maryland(PJM)real-time electricity market show that the proposed bidding method with HDBs can increase the bidding flexibility,thereby increasing the profits of state-of-the-art RL-based bidding methods. 展开更多
关键词 Electricity market artificial intelligence energy management neural network reinforcement learning highdimensional bid(HDB)
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人脐带间充质干细胞来源外泌体通过抑制Bid改善急性心肌梗死后铁死亡损伤
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作者 孙琅 彭伟 +6 位作者 沈晗 余云生 陈一欢 陈磊 丁英龙 陈月秋 沈振亚 《江苏大学学报(医学版)》 2025年第5期401-409,共9页
目的:探讨人脐带间充质干细胞来源的外泌体(human umbilical cord mesenchymal stem cells derived exosomes, hucMSC-Exo)对急性心肌梗死(acute myocardial infarction, AMI)的治疗作用及潜在的机制。方法:分离培养人脐带间充质干细胞(... 目的:探讨人脐带间充质干细胞来源的外泌体(human umbilical cord mesenchymal stem cells derived exosomes, hucMSC-Exo)对急性心肌梗死(acute myocardial infarction, AMI)的治疗作用及潜在的机制。方法:分离培养人脐带间充质干细胞(human umbilical cord mesenchymal stem cells, hucMSCs)并从培养液中提取外泌体。结扎小鼠左冠状动脉前降支构建AMI模型,将小鼠随机分为Sham组、AMI组、Exo组和Fer-1组,分别向各组缺血心肌边缘区注射PBS、PBS、hucMSC-Exo和经典的铁死亡抑制剂Ferrostatin-1(Fer-1)。通过对应试剂盒检测小鼠心肌组织抗氧化剂谷胱甘肽(GSH)和丙二醛(MDA)含量,并通过DHE探针检测活性氧(reactive oxygen species, ROS)水平,以评估小鼠心肌组织铁死亡严重程度;心脏超声、Masson染色评价小鼠心脏功能和纤维化水平。DMSO、Erastin和Erastin+hucMSC-Exo分别处理HL-1细胞并进行RNA测序,KEGG富集分析和聚类热图分析评估hucMSC-Exo治疗作用的潜在机制。CCK-8法检测hucMSC-Exo或hucMSC-Exo+BI-6C9联合处理后HL-1细胞的活力。qRT-PCR法检测小鼠梗死心肌组织中BH3相互作用结构域死亡激动剂(BH3-interaction domain death agonist,Bid)mRNA表达水平。结果:与AMI组相比,Exo组和Fer-1组小鼠心肌梗死区域MDA、ROS水平显著降低,GSH含量增加,心肌纤维化减轻,心脏功能改善;其中Exo组小鼠相比Fer-1组在AMI后第28天治疗效果更加显著。KEGG富集分析显示被hucMSC-Exo逆转的基因主要富集在p53信号通路;聚类热图分析p53相关基因发现,Erastin组HL-1细胞的Bid基因表达较对照组显著升高,Exo组HL-1细胞的Bid表达水平较Erastin组明显降低。Bid抑制剂BI-6C9处理HL-1细胞后,hucMSC-Exo的治疗作用未明显增加。AMI组小鼠心脏组织Bid mRNA表达水平相比对照组大幅升高,Exo组较AMI组显著降低。结论:hucMSC-Exo能够通过抑制Bid表达减轻AMI后的铁死亡,改善心肌损伤。 展开更多
关键词 急性心肌梗死 人脐带间充质干细胞来源的外泌体 铁死亡 BH3相互作用结构域死亡激动剂(Bid)
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Welcoming the new spring,inspiring the future—New year’s greetings from Journal of Automation and Intelligence
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作者 Yongduan Song 《Journal of Automation and Intelligence》 2025年第1期1-1,F0002,共2页
As time swiftly passes,we find ourselves welcoming another spring with renewed hope and energy.On this occasion of bidding farewell to the old and embracing the new,the editorial team of Journal of Automation and Inte... As time swiftly passes,we find ourselves welcoming another spring with renewed hope and energy.On this occasion of bidding farewell to the old and embracing the new,the editorial team of Journal of Automation and Intelligence(JAI)extends heartfelt gratitude and sincere wishes to all our editorial board members,peer reviewers,authors,readers,and friends from various fields who have supported the journal’s development! 展开更多
关键词 SPRING authors GREETINGS peer reviewers bidding farewell editorial board new year journal automation intelligence
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A risk-aware coordinated trading strategy for load aggregators with energy storage systems in the electricity spot market and demand response market
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作者 Ziyang Xiang Chunyi Huang +2 位作者 Kangping Li Chengmin Wang Pierluigi Siano 《iEnergy》 2025年第1期31-42,共12页
The demand response(DR)market,as a vital complement to the electricity spot market,plays a key role in evoking user-side regulation capability to mitigate system-level supply‒demand imbalances during extreme events.Wh... The demand response(DR)market,as a vital complement to the electricity spot market,plays a key role in evoking user-side regulation capability to mitigate system-level supply‒demand imbalances during extreme events.While the DR market offers the load aggregator(LA)additional profitable opportunities beyond the electricity spot market,it also introduces new trading risks due to the significant uncertainty in users’behaviors.Dispatching energy storage systems(ESSs)is an effective means to enhance the risk management capabilities of LAs;however,coordinating ESS operations with dual-market trading strategies remains an urgent challenge.To this end,this paper proposes a novel systematic risk-aware coordinated trading model for the LA in concurrently participating in the day-ahead electricity spot market and DR market,which incorporates the capacity allocation mechanism of ESS based on market clearing rules to jointly formulate bidding and pricing decisions for the dual market.First,the intrinsic coupling characteristics of the LA participating in the dual market are analyzed,and a joint optimization framework for formulating bidding and pricing strategies that integrates ESS facilities is proposed.Second,an uncertain user response model is developed based on price‒response mechanisms,and actual market settlement rules accounting for under-and over-responses are employed to calculate trading revenues,where possible revenue losses are quantified via conditional value at risk.Third,by imposing these terms and the capacity allocation mechanism of ESS,the risk-aware stochastic coordinated trading model of the LA is built,where the bidding and pricing strategies in the dual model that trade off risk and profit are derived.The simulation results of a case study validate the effectiveness of the proposed trading strategy in controlling trading risk and improving the trading income of the LA. 展开更多
关键词 Load aggregators demand response energy storage incentive pricing bidding strategy trading risk.
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Foreign Dominance and Local Resilience in Infrastructure Development:Evidence from Sarawak,East Malaysia
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作者 Yongqiang Wu Jinqiao Ling 《Journal of Architectural Research and Development》 2025年第5期69-83,共15页
As the Sarawak state government accelerates infrastructure development to stimulate economic growth,it has attracted numerous foreign construction enterprises-particularly Chinese firms-entering the market through low... As the Sarawak state government accelerates infrastructure development to stimulate economic growth,it has attracted numerous foreign construction enterprises-particularly Chinese firms-entering the market through low-cost bidding.However,this foreign-dominated model,while improving efficiency,has triggered local enterprise marginalization,industrial chain fragmentation,payment delays,and project setbacks,undermining the intended economic spillover effects of public investment.Grounded in policy analysis and field evidence within a dependency theory framework,this study evaluates the structural impacts of foreign participation on Sarawak’s local economy,uncovers governance gaps,and proposes strategies to enhance local economic resilience under open-market conditions.The findings reveal that foreign contractors(especially Chinese enterprises),despite their capital and technological advantages in dominating mega-projects,exhibit pervasive shortcomings:inadequate local participation(falling far below the 40%policy target),deficient knowledge transfer(local firms hold only 6%of green technology patents),significant value leakage(foreign profit repatriation dominated project value flows),and widespread execution delays/cost overruns due to underestimation of tropical climate-geological risks,institutional maladaptation,and resource misallocation.Drawing on experiences from the Philippines,Indonesia,and Vietnam,the study proposes systemic reforms:introducing a Low-Bid Risk Evaluation System(LRES),enforcing a mandatory Local Participation Index(LPI),establishing a Smart-Contract-Based Digital Payment Chain System(DPCS)to protect subcontractors,and requiring pre-bid local adaptation training for foreign contractors.These recommendations aim to construct“embedded institutional resilience,”transforming policy intent into executable governance mechanisms to ensure infrastructure investment genuinely serves Sarawak’s inclusive and sustainable growth. 展开更多
关键词 Foreign construction firms Local enterprise participation Low-cost bidding Industrial chain fragmentation Dependency theory Institutional capacity Economic resilience
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EXPO BIDS ADIEU
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作者 LI LI 《Beijing Review》 2010年第45期18-21,共4页
World Expo in Shanghai concludes amid praise and increased communication between China and the rest of the world The 184-day 2010 World Expo in Shanghai came to an end with a spectacular closing ceremony on the evenin... World Expo in Shanghai concludes amid praise and increased communication between China and the rest of the world The 184-day 2010 World Expo in Shanghai came to an end with a spectacular closing ceremony on the evening of October 31, which was attended by a host of world leaders. The first Expo staged in a 展开更多
关键词 EXPO bids ADIEU World
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Watchdog Barks At Big Bids
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作者 TAN WEI 《Beijing Review》 2008年第12期32-33,共2页
Turmoil caused by major refinancing plans of listed companies in the mainland stock market leads regulators to vow stricter approval processes On March 5,shareholders of Ping An Insurance(Group)Co.of China Ltd. (Ping ... Turmoil caused by major refinancing plans of listed companies in the mainland stock market leads regulators to vow stricter approval processes On March 5,shareholders of Ping An Insurance(Group)Co.of China Ltd. (Ping An)passed a refinancing plan of 120 billion yuan($17 billion)at its first interim meeting of the year with an overwhelming 92 percent in favor. 展开更多
关键词 NASDAQ Watchdog Barks At Big bids
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促凋亡蛋白Bid/C1q对酮病奶牛脂肪细胞凋亡及炎症的影响
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作者 李晴 孙晗冰 +2 位作者 向念 许秋实 徐闯 《中国兽医学报》 CSCD 北大核心 2024年第11期2477-2482,共6页
奶牛酮病经常伴随着脂肪组织中凋亡及炎症的反应。为了研究促凋亡蛋白Bid对酮病奶牛脂肪细胞凋亡的影响,本研究通过TUNEL染色技术对脂肪组织进行染色,观察酮病奶牛脂肪组织凋亡变化。体内试验中,通过Western blot检测健康奶牛及酮病奶... 奶牛酮病经常伴随着脂肪组织中凋亡及炎症的反应。为了研究促凋亡蛋白Bid对酮病奶牛脂肪细胞凋亡的影响,本研究通过TUNEL染色技术对脂肪组织进行染色,观察酮病奶牛脂肪组织凋亡变化。体内试验中,通过Western blot检测健康奶牛及酮病奶牛脂肪组织中凋亡相关因子Bid、Bax、C-Caspase-3、Bcl-2及炎症标志因子C1q、IL-1β、IL-10和IL-6的蛋白表达。体外试验中,本研究通过体外培养原代牛脂肪细胞抑制Bid,并添加异丙肾上腺素(ISO)构建脂肪细胞脂解模型,采用Western blot技术检测脂肪细胞中凋亡相关分子及炎症相关分子的蛋白表达水平。TUNEL染色结果显示:与健康奶牛相比,酮病奶牛组脂肪组织明显发生了凋亡,酮病奶牛脂肪组织中的促凋亡相关因子Bid、Bax和C-Caspase-3及促炎症标志因子TNF-α、IL-1β、和IL-6的蛋白表达均显著升高,补体C1q、抗凋亡因子Bcl-2及抗炎症因子IL-10显著降低。体外结果显示:与对照组相比,ISO组处理的脂肪细胞凋亡和炎症相关因子蛋白表达水平明显升高;而添加Bid抑制剂处理组脂肪细胞凋亡和炎症相关因子蛋白表达水平明显降低。上述结果显示,抑制Bid可以缓解ISO对脂肪细胞的凋亡及炎症反应。进一步明确了Bid/C1q对酮病奶牛脂肪组织及细胞凋亡及炎症的调控作用。 展开更多
关键词 奶牛酮病 脂肪细胞 BID 凋亡 炎症
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Construction of the“Micro-Ideological and Political”Model for Ideological and Political Teaching Reform in Bidding and Contract Management Courses
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作者 Dongchun Xia 《Journal of Contemporary Educational Research》 2024年第11期326-332,共7页
In the context of deepening educational reforms,the significance of ideological and political education has become increasingly prominent,emerging as a crucial aspect of enhancing students’comprehensive qualities.To ... In the context of deepening educational reforms,the significance of ideological and political education has become increasingly prominent,emerging as a crucial aspect of enhancing students’comprehensive qualities.To ensure the orderly progress of ideological and political education in courses,it is essential to actively promote the integration of ideological and political elements within coursework.This integration aims to align professional course education with ideological and political education,fostering a collaborative educational model.Therefore,in the teaching of bidding and contract management courses,constructing a“micro-ideological and political”teaching model has emerged as a critical component.The implementation of this model facilitates the seamless integration of bidding and contract management coursework with ideological and political education,thereby elevating the teaching quality of these courses.Additionally,it leverages the synergistic role of coursework and ideology in educating students,ultimately enhancing the quality and level of talent cultivation.Hence,this paper proposes strategies for constructing a“microideological and political”model within the context of ideological and political teaching reforms in bidding and contract management courses,inviting discussion and exchange on this topic. 展开更多
关键词 Bidding and contract management courses Ideological and political elements “Micro-ideological and political”model
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促凋亡蛋白Bid诱导肝细胞凋亡的机制 被引量:12
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作者 白莉 曹传平 +1 位作者 张映辉 毛高平 《中国生物化学与分子生物学报》 CAS CSCD 北大核心 2004年第5期670-674,共5页
为研究促凋亡蛋白Bid对肝细胞凋亡过程中的调节机制 ,在体内和体外分别用TNF α或抗Fas抗体诱导小鼠肝细胞凋亡 .免疫荧光染色观察Bax转位和构象变化 ;采用ELISA检测caspase 3和 8的活性 ;Western印迹测定Bid和Bax的裂解活化及Bax的转... 为研究促凋亡蛋白Bid对肝细胞凋亡过程中的调节机制 ,在体内和体外分别用TNF α或抗Fas抗体诱导小鼠肝细胞凋亡 .免疫荧光染色观察Bax转位和构象变化 ;采用ELISA检测caspase 3和 8的活性 ;Western印迹测定Bid和Bax的裂解活化及Bax的转位和插入 .结果显示 :TNF α或抗Fas抗体通过激活Bid导致Bax转位和构象变化 ,使Bax得以插入线粒体膜诱导肝细胞凋亡 .阻断Bid的作用 ,则Bax的转位和插入明显被削弱 ,肝细胞的凋亡受到抑制 .提示由死亡受体诱导的肝细胞调亡可能受Bid调节 ,Bax转位和插入依赖于Bid . 展开更多
关键词 细胞调亡 BID 肝细胞 转位 插入
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学龄儿童的抑郁 被引量:33
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作者 郭兰婷 单友荷 +1 位作者 周在东 江晓云 《中国心理卫生杂志》 CSSCI CSCD 北大核心 1998年第3期151-154,共4页
322名10~12岁小学五、六年级学生填写了Bulevue抑郁量表(BID)。抑郁症状的出现率3.4%~11.5%,有自杀观念者5.9%。自杀观念与总分、心境恶劣、自卑和自杀、攻击性行为和社会交往4个因子的相关程度最... 322名10~12岁小学五、六年级学生填写了Bulevue抑郁量表(BID)。抑郁症状的出现率3.4%~11.5%,有自杀观念者5.9%。自杀观念与总分、心境恶劣、自卑和自杀、攻击性行为和社会交往4个因子的相关程度最高。322人中10人(3.1%)为抑郁症检出者,临床表现与成人抑郁症相似,其中8人有自杀观念。结果提示抑郁情绪、自杀观念、抑郁症在学龄儿童中的出现率较高,应加强预防和治疗。 展开更多
关键词 抑郁症 儿童 BID
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白英水提物对胃癌SGC-7901细胞凋亡与基因bcl-xl/bid表达的影响 被引量:13
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作者 余乐涵 许宝华 +3 位作者 吴剑 李华 涂硕 万福生 《中成药》 CAS CSCD 北大核心 2008年第12期1744-1748,共5页
目的:观察白英水提物对胃癌SGC-7901细胞凋亡及基因bcl-xl、bid表达的影响。方法:常规法制备白英水提物,实验分正常对照组、白英处理组。白英处理组加入白英水提物终浓度分别为12.5mg/mL、25mg/mL和50mg/mL。流式细胞仪检测细胞凋亡,半... 目的:观察白英水提物对胃癌SGC-7901细胞凋亡及基因bcl-xl、bid表达的影响。方法:常规法制备白英水提物,实验分正常对照组、白英处理组。白英处理组加入白英水提物终浓度分别为12.5mg/mL、25mg/mL和50mg/mL。流式细胞仪检测细胞凋亡,半定量RT-PCR和免疫化学分析细胞中bcl-xl、bid基因表达。结果:与正常对照组比较,白英处理组SGC-7901细胞增殖有显著性下降,细胞凋亡显著性增多(P<0.05);基因bcl-xl mRNA和Bcl-xl蛋白表达降低、bidmR-NA和Bid蛋白表达升高(P<0.05),并随白英浓度增加而加强。结论:白英水提物能诱导人胃癌SGC-7901细胞凋亡和抑制增殖,且有一定的量效关系,其作用机制可能与调控bcl-xl、bid基因表达有关。 展开更多
关键词 白英 SGC-7901细胞 凋亡 bcl—xl/bid 基因表达
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FAST HUGS BID理念在ICU机械通气患者中的应用效果分析 被引量:6
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作者 梁园园 权明桃 +3 位作者 傅小云 游恩丽 张华 李静 《重庆医科大学学报》 CAS CSCD 北大核心 2015年第4期638-640,共3页
目的:探讨FAST HUGS BID理念在ICU机械通气患者中的应用效果。方法:将收入我科的气管插管行机械通气并计划性拔管成功患者,按照入科时间分为对照组和试验组,对照组采用危重患者护理常规,试验组在护理常规基础上,引入FAST HUGS BID理念,... 目的:探讨FAST HUGS BID理念在ICU机械通气患者中的应用效果。方法:将收入我科的气管插管行机械通气并计划性拔管成功患者,按照入科时间分为对照组和试验组,对照组采用危重患者护理常规,试验组在护理常规基础上,引入FAST HUGS BID理念,比较2组患者气管插管的非计划性拔管率及平均留置时间。结果:对照组患者气管插管的非计划性拔管率为1.73%,平均留置时间(7.59±6.06)d,试验组患者气管插管的非计划性拔管率0.93%,平均留置时间(5.04±4.03)d。结论:FAST HUGS BID理念降低了气管插管患者的非计划性拔管率,明显缩短了气管插管的平均留置时间,提高了ICU重症患者的监护质量,是一种科学、有效的管理方法。 展开更多
关键词 FAST HUGS BID ICU 机械通气 效果
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截短型人Bid融合蛋白对HeLa细胞的促凋亡作用 被引量:8
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作者 裘秀春 于翠娟 +6 位作者 许彦鸣 贾林涛 曹云新 王智 王成济 范清宇 杨安钢 《第四军医大学学报》 北大核心 2003年第21期1963-1966,共4页
目的 :观察截短型人bid及其N端融合蛋白的表达对HeLa细胞的促凋亡作用 .方法 :通过反转录PCR方法获取人全长bid基因 ,再经PCR方法克隆得到截短型bid(trun catedbid ,tbid)基因及其N端融合蛋白基因插入pcDNA3真核表达载体 ,脂质体法转染H... 目的 :观察截短型人bid及其N端融合蛋白的表达对HeLa细胞的促凋亡作用 .方法 :通过反转录PCR方法获取人全长bid基因 ,再经PCR方法克隆得到截短型bid(trun catedbid ,tbid)基因及其N端融合蛋白基因插入pcDNA3真核表达载体 ,脂质体法转染HeLa细胞 ,通过间接免疫荧光 ,电子显微镜以及流式细胞仪等方法观察被转染细胞的生长及凋亡状况 .结果 :成功获得人bid基因 ,构建了tbid基因及tbid与绿脓杆菌外毒素 (PE)转膜结构域部分肽段的融合蛋白基因的真核表达载体 (pcDNA3 tbid6 1 ,pcDNA3 PE tbid6 1 ) .与对照组相比 ,在转染后 1 2~ 4 8h内两种基因的表达均导致HeLa细胞发生凋亡 ,且两者活性相当 .结论 展开更多
关键词 截短型人Bid融合蛋白 细胞凋亡 HELA细胞 促凋亡作用 bid基因 基因表达
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截短型人bid基因的克隆、表达和促凋亡作用的研究 被引量:6
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作者 裘秀春 于翠娟 +6 位作者 孟艳玲 许彦鸣 赵晶 金明 王成济 范清宇 杨安钢 《细胞与分子免疫学杂志》 CAS CSCD 北大核心 2004年第1期19-22,共4页
目的 :探讨截短型bid(truncatedbid ,tbid)基因的表达对Hela细胞的促进凋亡活性。方法 :用RT PCR法克隆人全长bid基因 ,测序正确后 ,通过PCR截去编码N末端 6 0个氨基酸残基的基因 ,而获得tbid基因。将其克隆入含绿色荧光蛋白(GFP)基因... 目的 :探讨截短型bid(truncatedbid ,tbid)基因的表达对Hela细胞的促进凋亡活性。方法 :用RT PCR法克隆人全长bid基因 ,测序正确后 ,通过PCR截去编码N末端 6 0个氨基酸残基的基因 ,而获得tbid基因。将其克隆入含绿色荧光蛋白(GFP)基因的真核表达载体pIRES2 EGFP中 ,用脂质体法转染Hela细胞。通过荧光显微镜、电子显微镜观察和TUNEL检测法 ,检测目的基因的表达对转染细胞的形态及生长状况的影响。结果 :成功地构建了tbid基因的真核表达载体。以其转染Hela细胞后 ,tbid基因在细胞中得到表达 ,随后引起细胞荧光强度下降 ,生长状况不良甚至死亡。电镜观察及TUNEL检测的结果显示 ,许多细胞呈典型的凋亡特征。结论 展开更多
关键词 bid基因 细胞凋亡 绿色荧光蛋白 HELA细胞
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枸杞丹参对RCS大鼠视网膜组织中Bcl-2、Bid表达的影响 被引量:8
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作者 彭俊 王英 +5 位作者 蒋鹏飞 刘家琪 潘坤 周亚莎 徐剑 彭清华 《世界科学技术-中医药现代化》 CSCD 北大核心 2020年第4期1166-1175,共10页
目的观察枸杞加丹参对视网膜色素变性大鼠视网膜Bcl-2、Bid表达的影响。方法实验对象分五组。空白组:RCS(rdy+/+,p+/+)大鼠,8只,雌雄均4只,灌胃生理盐水;将RCS(rdy-/-,p-/-)雌性及雄性大鼠均随机分为4组,分别为:模型组、枸杞组、丹参组... 目的观察枸杞加丹参对视网膜色素变性大鼠视网膜Bcl-2、Bid表达的影响。方法实验对象分五组。空白组:RCS(rdy+/+,p+/+)大鼠,8只,雌雄均4只,灌胃生理盐水;将RCS(rdy-/-,p-/-)雌性及雄性大鼠均随机分为4组,分别为:模型组、枸杞组、丹参组和枸杞加丹参组(杞参组),每组雌雄均4只,共8只,分别给予灌胃生理盐水、生理盐水、枸杞、丹参、枸杞加丹参。灌胃28天后,免疫组化、Real-time PCR、WB检测各组RCS大鼠视网膜Bcl-2、Bid的相对表达量。结果①免疫组化检测显示:RCS(rdy-/-,p-/-)杞参组大鼠视网膜上Bcl-2蛋白阳性表达较RCS(rdy-/-,p-/-)大鼠模型组明显增多;RCS(rdy-/-,p-/-)杞参组大鼠视网膜上Bid蛋白阳性表达较RCS(rdy-/-,p-/-)大鼠模型组明显减少,但较RCS(rdy+/+,p+/+)大鼠空白组多。②RT-qPCR检测显示:RCS(rdy-/-,p-/-)杞参组Bcl-2 mRNA相对表达量明显高于RCS(rdy-/-,p-/-)模型组,差异有统计学意义(P<0.05),且接近RCS(rdy+/+,p+/+)大鼠空白对照组。RCS(rdy-/-,p-/-)杞参组Bid mRNA相对表达量明显低于RCS(rdy-/-,p-/-)模型组,差异有统计学意义(P<0.05),且接近RCS(rdy+/+,p+/+)大鼠空白对照组。③WB检测显示:RCS(rdy-/-,p-/-)杞参组Bcl-2蛋白相对表达量明显高于RCS(rdy-/-,p-/-)模型组,差异有统计学意义(P<0.05),且接近RCS(rdy+/+,p+/+)大鼠空白对照组。RCS(rdy-/-,p-/-)杞参组Bid蛋白相对表达量明显低于RCS(rdy-/-,p-/-)模型组,差异有统计学意义(P<0.05),且接近RCS(rdy+/+,p+/+)大鼠空白对照组。结论视网膜色素变性经典动物模型RCS(rdy-/-,p-/-)大鼠视网膜组织中Bcl-2表达下降、Bid表达上升;枸杞加丹参灌胃促进RCS(rdy-/-,p-/-)大鼠视网膜抗凋亡因子Bcl-2表达上调,同时,下调促凋亡因子Bid表达,在抗线粒体凋亡途径上减轻视网膜色素变性大鼠的视网膜细胞凋亡,从而保护视功能。 展开更多
关键词 视网膜色素变性 RCS大鼠 BCL-2 BID
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细胞凋亡过程中Bid蛋白在活细胞内的动态分布 被引量:9
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作者 陈同生 王进军 +1 位作者 邢达 王方 《激光生物学报》 CAS CSCD 2005年第4期279-282,共4页
当细胞暴露于凋亡诱导因子如肿瘤坏死因子(tumornecrosisfactor-α,TNF-α)等的环境中,被激活的凋亡酶Caspase8将Bid蛋白切割成两个片断,随后其C-端片段转移到线粒体上诱发细胞色素c的释放,最终引起细胞凋亡。虽然关于Bid蛋白的研究已... 当细胞暴露于凋亡诱导因子如肿瘤坏死因子(tumornecrosisfactor-α,TNF-α)等的环境中,被激活的凋亡酶Caspase8将Bid蛋白切割成两个片断,随后其C-端片段转移到线粒体上诱发细胞色素c的释放,最终引起细胞凋亡。虽然关于Bid蛋白的研究已经取得了很大进展,但是Bid蛋白是如何转移到线粒体以及如何引起细胞色素c释放等许多问题尚未十分明了。为了进一步对Bid蛋白的生物学行为进行研究,特别是在无损伤、活细胞生理条件下,本实验采用了荧光蛋白标记和荧光成像技术对凋亡过程中Bid蛋白在活细胞内分布的动态过程进行了初步研究。 展开更多
关键词 Bid蛋白 凋亡 活细胞 荧光蛋白
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Bcl-2家族蛋白在维生素E琥珀酸酯诱导人胃癌细胞凋亡中的作用 被引量:4
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作者 张晓华 苑林宏 +2 位作者 单毓娟 张岭 吴坤 《营养学报》 CAS CSCD 北大核心 2007年第1期31-34,共4页
目的:研究Bcl-2家族蛋白在维生素E琥珀酸酯(RRR-α-tocopheryl succinate,α-TOS;vitamin E succinate,VES)诱导人胃癌SGC-7901细胞凋亡线粒体途径中的作用。方法:采用噻唑蓝(MTT)法测定VES对SGC-7901细胞的半数抑制浓度(IC50值);吖啶橙... 目的:研究Bcl-2家族蛋白在维生素E琥珀酸酯(RRR-α-tocopheryl succinate,α-TOS;vitamin E succinate,VES)诱导人胃癌SGC-7901细胞凋亡线粒体途径中的作用。方法:采用噻唑蓝(MTT)法测定VES对SGC-7901细胞的半数抑制浓度(IC50值);吖啶橙/溴化乙啶(AO/EB)染色观察细胞凋亡;Mito Tracker Red CMXRos染色观察线粒体膜电位(ΔΨm)的改变;Western Blot法检测不同剂量VES对人胃癌SGC-7901细胞Bid、Bax、Bcl-2蛋白表达和细胞色素C(cytochrome C,Cyt C)蛋白表达与定位的影响。结果:VES对SGC-7901细胞的IC50值为101.45μg/ml;VES可引起SGC-7901细胞发生凋亡和线粒体膜电位下降;并引起Cyt C蛋白在细胞内重新定位、Bid蛋白剪切活化、Bax蛋白表达增加和Bcl-2蛋白表达减少。结论:VES可抑制SGC-7901细胞的生长,并通过线粒体途径诱导凋亡,其机制可能是通过剪切活化Bid蛋白、上调Bax/Bcl-2相对水平来实现的。 展开更多
关键词 维生素E琥珀酸酯 胃癌 BID BAX Bol-2 CYT C
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