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高粱中FoMV诱导的基因沉默体系的构建
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作者 张丽 袁兴帅 +1 位作者 李淑玲 邵玉涛 《分子植物育种》 北大核心 2025年第5期1464-1468,共5页
遗传转化技术是研究基因功能的重要方法,然而高粱中遗传转化体系尚不成熟,病毒介导的基因沉默体系(VIGS)将为研究高粱基因功能提供一条便利可行的途径。本研究以狗尾草花叶病毒(FoMV)为基础,以高粱品种‘BTx623’为研究材料,利用FoMV-s... 遗传转化技术是研究基因功能的重要方法,然而高粱中遗传转化体系尚不成熟,病毒介导的基因沉默体系(VIGS)将为研究高粱基因功能提供一条便利可行的途径。本研究以狗尾草花叶病毒(FoMV)为基础,以高粱品种‘BTx623’为研究材料,利用FoMV-sg载体沉默八氢番茄红素脱氢酶(PDS)基因,构建病毒诱导的基因沉默体系。注射烟草14 d后病毒扩增良好,将烟草叶片研磨并涂抹二叶期高粱幼苗,涂抹28 d后,FoMV-sg-PDS组出现明显白化表型;提取叶片RNA,利用RT-PCR的方法进行SbPDS基因沉默效果检验,结果表明,与对照(Mock)和空载(FoMV-sg)相比,FoMV-sg-PDS组中SbPDS基因条带明显变弱,发生了沉默反应。本研究初步构建了高粱中病毒介导的基因沉默体系,可用于高粱相关基因的功能研究。 展开更多
关键词 高粱(Sorghum bicolor) 狗尾草花叶病毒载体 SbPDS基因 基因沉默体系
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Effective removal of fluoride and arsenic from groundwater via integrated biosorption and membrane ultrafiltration
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作者 Bukke Vani Mannem Hymavathi +2 位作者 Swayampakula Kalyani Nivedita Sahu Sundergopal Sridhar 《Water Science and Engineering》 2025年第1期30-40,共11页
Fluoride(F^(-))and arsenic,present as As(Ⅲ)and As(Ⅴ),are widespread toxins in groundwater across India,as well as in other countries or regions like Pakistan,China,Kenya,Africa,Thailand,and Latin America.Their prese... Fluoride(F^(-))and arsenic,present as As(Ⅲ)and As(Ⅴ),are widespread toxins in groundwater across India,as well as in other countries or regions like Pakistan,China,Kenya,Africa,Thailand,and Latin America.Their presence in water resources poses significant environmental and health risks,including fluorosis and arsenicosis.To address this issue,this study developed an integrated process combining biosorbents and ultrafiltration(UF)for the removal of F^(-),As,and turbidity from contaminated water.Laboratory-scale adsorption experiments were conducted using low-cost biosorbents with different biosorbent dosages,specifically Moringa oleifera seed powder(MSP)and sorghum bicolor husk(SBH),along with sand as a binding medium.F^(-)and As concentrations ranging from 2 to 10 mg/L and 3 to 12 mg/L,respectively,were investigated.Biosorbents and their different combinations were tested to determine their efficacy in removing dissolved F^(-)and As.The results showed that a blend of 10-g/L MSP with SBH achieved the highest F^(-)(97.20%)and As(78.63%)removal efficiencies.Subsequent treatment with a UF membrane effectively reduced turbidity and colloidal impurities in the treated water,achieving a maximum turbidity removal efficiency of 95.40%.Equilibrium kinetic and isotherm models were employed to analyze the experimental data,demonstrating good fit.Preliminary cost analysis indicated that the hybrid technology is economically viable and suitable for the separation of hazardous contaminants from aqueous solutions.This study underscores the potential of inexpensive biosorption technologies in providing clean and safe drinking water,particularly in industrial,rural,and urban areas. 展开更多
关键词 FLUORIDE ARSENIC BIOSORBENTS Moringa oleifera Sorghum bicolor husk SAND Membrane treatment
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Genome-Wide Identification and Functional Characterization of UGT Gene Family in Sorghum bicolor with Insights into SbUGT12’s Role in C4 Photosynthesis
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作者 Wenxiang Zhang Wenning Cui +8 位作者 Juan Huang Zhangen Lu Kuijing Liang LingbaoWang ShanshanWei Liran Shi Huifen Li Xiaoli Guo Jianhui Ma 《Phyton-International Journal of Experimental Botany》 2025年第12期3893-3912,共20页
UDP-glycosyltransferases(UGTs)play essential roles in plant secondary metabolism and stress responses,yet their composition and functions in Sorghum bicolor,a model C4 plant,remain inadequately characterized.This stud... UDP-glycosyltransferases(UGTs)play essential roles in plant secondary metabolism and stress responses,yet their composition and functions in Sorghum bicolor,a model C4 plant,remain inadequately characterized.This study identified 196 SbUGT genes distributed across all 10 chromosomes and classified them into 16 subfamilies(A–P)through phylogenetic analysis.Among these,61.2%were intronless,and 10 conserved motifs,including the UGT-specific PSPG box,were identified.Synteny analysis using MCScanX revealed 12 segmental duplication events and conserved syntenic relationships with other Poaceae species(rice,maize,and barley).Promoter analysis uncovered 125 distinct cis-acting elements,predominantly associated with stress and hormone responses,as well as MYB/MYC binding sites.Weighted gene co-expression network analysis(WGCNA)indicated that genes in cluster C2 were highly expressed in leaves and correlated with the C4 photosynthetic pathway.Within this cluster,SbUGT12 was identified as a hub gene,demonstrating strong binding affinity to UDP-glucose and forming a co-expression network with key C4 photosynthetic genes.Molecular docking further confirmed its binding capacity with four C4-related compounds.These findings provide insights into the evolution and function of the SbUGT family and suggest a regulatory role for SbUGT12 in C4 photosynthesis,offering genetic resources for improving stress tolerance and photosynthetic efficiency in sorghum. 展开更多
关键词 Sorghum bicolor SbUGTs genome-wide analysis tissue-specific expression cis-element analysis WGCNA C4 photosynthesis molecular docking
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High color quality laser-driven white lighting enabled by reflective bicolor phosphor-in-glass film converter
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作者 Yun Mou Ben Tian +6 位作者 Xin Liu Jiuzhou Zhao Xinyuan Wang Deyi Chen Jian Xu Xinzhong Wang Yang Peng 《Journal of Rare Earths》 2025年第9期1844-1851,共8页
All-inorganic reflective phosphor-in-glass film(PiGF) converter has garnered widespread attentions for high brightness laser-driven white lighting,while its poor color quality and low luminescence stability have been ... All-inorganic reflective phosphor-in-glass film(PiGF) converter has garnered widespread attentions for high brightness laser-driven white lighting,while its poor color quality and low luminescence stability have been inevitable roadblocks.Herein,the bicolor PiGF containing green-emitting Y3Al3.08Ga1.92O12:Ce3+(YAGG) and red-emitting CaAlSiN_(3):Eu^(2+)(CASN) phosphors bonded on Al2O3substrate was prepared for enabling high color quality laser-driven white lighting in reflective configuration.The bicolor PiGF has high quantum efficiency and good structure stability.By optimizing the CASN content,PiGF thickness and Al_(2)O_(3) content,the reflective bicolor PiGF based white laser diode(LD)displays good luminescence performance with a luminous flux of 451.5 lm and a luminous efficacy of142.3 lm/W and high color quality with a color rendering index(CRI) of 85.3 and a correlated color temperature(CCT) of 5177 K under the incident laser power of 3.15 W,and still has excellent luminescence and color stabilities(CRI and CCT) under the continuous laser excitation of 5.61 W,attributed to the good thermal conductivity and high reflectivity of Al_(2)O_(3) substrate and scattering enhancement effect of Al_(2)O_(3) particles.It can be foreseen that the reflective bicolor PiGF converter provides a promising strategy for enabling high quality laser-driven white lighting. 展开更多
关键词 Laser-driven white lighting Phosphor-in-glass film Bicolor reflective phosphor converter Color quality Optical performance Rare earths
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能源作物资源现状与发展前景 被引量:92
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作者 谢光辉 郭兴强 +3 位作者 王鑫 丁荣娥 胡林 程序 《资源科学》 CSSCI CSCD 北大核心 2007年第5期74-80,共7页
能源作物是生物质能源发展的基础环节,本文就国内外能源作物研究现状与前景进行综合述评。分析了能源作物的基本概念和分类,明确了适合中国国情的能源作物概念,归纳了当前淀粉与糖料作物、油脂作物和木质纤维素作物三类能源作物研究与... 能源作物是生物质能源发展的基础环节,本文就国内外能源作物研究现状与前景进行综合述评。分析了能源作物的基本概念和分类,明确了适合中国国情的能源作物概念,归纳了当前淀粉与糖料作物、油脂作物和木质纤维素作物三类能源作物研究与应用现状。重点介绍了当前几种受国内外重视的新型能源作物甜高粱(Sorghum bicolor)、木薯(Manihot esculenta)、柳枝稷(Panicum virgatum)、芒属植物(Miscanthusspp.)和柳属植物(Salixspp.)的发展现状和前景。从土地的承载能力和对环境的影响方面分析了中国发展发展能源作物的存在的问题与前景,明确提出,中国要坚持利用边际性土地、与生态恢复相结合发展能源作物的原则。指出中国能源作物种类筛选及品种改良至关重要,选择目标上应重视高光效、多年生、以收获营养器官为收获对象和高度抗逆等特征,以期形成具有地区适应性且收获季节多样化的系列能源作物种类与品种。 展开更多
关键词 能源作物 甜高粱(Sorghum bicolor) 木薯(Manihot esculenta) 柳枝稷(Panicum virgatum) 芒属 (Miscanthus SPP ) 柳属(Salix spp.)
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高粱受精过程及其经历的时间 被引量:5
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作者 李冉俐 申家恒 +2 位作者 贾媛 李伟 王黎明 《作物学报》 CAS CSCD 北大核心 2009年第12期2234-2242,共9页
应用常规石蜡切片技术,对高粱花粉管生长途径、双受精过程及其经历的时间进行研究。结果表明,高粱开花即授粉,花粉随即萌发,花粉管进入羽状柱头分支结构的细胞间隙,向下生长于花柱至子房顶部引导组织的细胞间隙中;进入子房腔后,在子房... 应用常规石蜡切片技术,对高粱花粉管生长途径、双受精过程及其经历的时间进行研究。结果表明,高粱开花即授粉,花粉随即萌发,花粉管进入羽状柱头分支结构的细胞间隙,向下生长于花柱至子房顶部引导组织的细胞间隙中;进入子房腔后,在子房壁与外珠被之间的缝隙中向珠孔方向生长;花粉管破坏一个助细胞后,在卵细胞与中央细胞之间的空隙中释放连续的球状内容物;两个精核分别进入卵细胞和中央细胞分别与卵核和极核融合。受精过程经历的时间为开花后0.5~3h左右,花粉管破坏一个助细胞进入胚囊,并释放精子;1~3h精核与极核融合形成初生胚乳核,精卵融合形成合子;4h左右初生胚乳核分裂;14h左右合子分裂。4~14h为合子静止期,该期为物理、化学诱变及花粉管通道法转基因技术实施的合适时间。 展开更多
关键词 高粱(Sorghum BICOLOR L.Moench) 花粉管生长途径 受精过程 受精经历时间 合子静止期
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Comparative Study on Four Methods for Quick Extraction of Sorghum Genomic DNA 被引量:3
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作者 高建明 夏卜咸 +5 位作者 杨洪 曲荣桂 桂枝 罗峰 裴忠有 孙守钧 《Agricultural Science & Technology》 CAS 2011年第5期686-687,744,共3页
[Objective] This study was to find out a quick,simple,and low-cost method for the extraction of sorghum genomic DNA.[Method] Four plant genomic DNA extraction methods based on CTAB,including liquid nitrogen grinding m... [Objective] This study was to find out a quick,simple,and low-cost method for the extraction of sorghum genomic DNA.[Method] Four plant genomic DNA extraction methods based on CTAB,including liquid nitrogen grinding method(method I),buffer grinding method(method II),drying grinding method(method III)and directly grinding method(method IV),were used to extract the sorghum genomic DNA from leaves;further the quantity and quality of the yielded DNA were detected by gel electrophoresis,SSR-PCR and SRAP-PCR.[Result] These four methods performed no remarkable difference in DNA product.The method I and method II produced DNA with higher purity and better integrity,which,especially from method I,is effective for SRAP-PCR and SSR-PCR.While the DNA extracted via method III and method IV had less integrality and lower purity,and only effective in SSR-PCR.[Conclusion] Enough amount of sorghum genomic DNA to perform tens of PCR could be quickly extracted using all these four methods.The DNA obtained via method I and method II had a broader application spectrum(SRAP,RAPD,ISSR and SSR)than that via method III and method IV which is only proper for PCR targeting small DNA fragments(SSR). 展开更多
关键词 Sorghum bicolor LEAF Genomic DNA CTAB method Quick extraction
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A New Record of Anguillid Fish in Lancangjiang River,China:Anguilla bicolor 被引量:3
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作者 陈自明 张晓妍 +2 位作者 祁文龙 邓秀梅 肖蘅 《Zoological Research》 CAS CSCD 北大核心 2010年第4期444-445,共2页
One specimen was collected from Lancangjiang River in Jinghong,China on August 1st,2006.It is identified as Anguilla bicolor McClelland,1844,a new record of Anguillidae species occurring in Lancangjiang River in China... One specimen was collected from Lancangjiang River in Jinghong,China on August 1st,2006.It is identified as Anguilla bicolor McClelland,1844,a new record of Anguillidae species occurring in Lancangjiang River in China.It could be distinguished from other Anguilla species by the character as:dorsal-fin origin located above vent vertically.It is an active nocturnal forager,feeding largely on a diet of crustaceans and mollusks. 展开更多
关键词 Anguilla bicolor FISH New species record Lancangjiang River China
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品种和种植密度对高粱主要农艺性状和产量的影响 被引量:9
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作者 韩永亮 崔江慧 +2 位作者 罗河月 方路斌 常金华 《湖北农业科学》 2021年第17期19-22,共4页
为探索冀中南地区高粱[Sorghum bicolor(L.)Moench]种植的适合品种和适宜密度,以河北农业大学选育的品种农大红3号、自选品系矮秆1号、红茅梁6号选系为试验材料,采用双因素完全随机设计,设3个密度处理,对3个品种进行比较试验。结果表明... 为探索冀中南地区高粱[Sorghum bicolor(L.)Moench]种植的适合品种和适宜密度,以河北农业大学选育的品种农大红3号、自选品系矮秆1号、红茅梁6号选系为试验材料,采用双因素完全随机设计,设3个密度处理,对3个品种进行比较试验。结果表明,农大红3号产量显著高于其他品种;种植密度120000株/hm^(2)时,产量水平最高达10270.50 kg/hm^(2)。农大红3号适合冀中南地区种植,密度约120000株/hm^(2)可实现较高产量。 展开更多
关键词 高粱[Sorghum bicolor(L.)Moench] 种植密度 品种 产量 农艺性状
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青贮甜高粱替代青贮玉米对羔羊生产性能和养分消化代谢的影响 被引量:8
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作者 王宏博 李维红 +2 位作者 杨晓玲 高雅琴 席斌 《湖北农业科学》 2020年第16期112-114,125,共4页
为了研究饲用甜高粱[Sorghum bicolor(L.)Moench]青贮替代玉米青贮对羔羊生产性能及养分消化代谢的影响,选取3月龄(28.10±0.32 kg)健康断奶羔羊30只,随机分为3组(Ⅰ为玉米青贮组、Ⅱ为甜高粱玉米青贮组、Ⅲ为甜高粱青贮组),每组10... 为了研究饲用甜高粱[Sorghum bicolor(L.)Moench]青贮替代玉米青贮对羔羊生产性能及养分消化代谢的影响,选取3月龄(28.10±0.32 kg)健康断奶羔羊30只,随机分为3组(Ⅰ为玉米青贮组、Ⅱ为甜高粱玉米青贮组、Ⅲ为甜高粱青贮组),每组10只,隔栏单独进行饲养和消化代谢试验。结果表明,饲用甜高粱青贮替代玉米青贮对羔羊的生产性能无显著影响(P>0.05)。Ⅱ组羔羊对磷的表观消化率极显著高于Ⅲ组(P<0.01),而Ⅰ组羔羊对磷的表观消化率与Ⅱ组差异不显著(P>0.05)。羔羊对玉米青贮和50%甜高粱青贮替代50%玉米青贮的其他养分的表观消化率均未产生显著影响(P>0.05),而对其他营养素未产生显著影响。说明饲用甜高粱青贮可以作为肉羊的优质饲料,50%的饲用甜高粱秸秆和青贮分别替代玉米青贮,可提高N的存留率和羔羊对CP消化率。 展开更多
关键词 饲用青贮甜高粱[Sorghum bicolor(L.)Moench] 青贮玉米 羔羊 生产性能 消化代谢
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高粱SSR标记的筛选及PCR反应程序的优化 被引量:1
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作者 韩芸 高建明 +2 位作者 孙守钧 裴忠有 罗峰 《湖北农业科学》 北大核心 2012年第17期3869-3872,共4页
以BJ-299、Tx622B、W456、忻粱52和Roma 5个高粱[Sorghum bicolor(L.)Moench]品种的基因组DNA为模板进行PCR扩增以筛选SSR分子标记引物。设计不同的退火温度和扩增程序,对250对SSR引物进行筛选和反应程序的优化。结果表明,250对SSR引物... 以BJ-299、Tx622B、W456、忻粱52和Roma 5个高粱[Sorghum bicolor(L.)Moench]品种的基因组DNA为模板进行PCR扩增以筛选SSR分子标记引物。设计不同的退火温度和扩增程序,对250对SSR引物进行筛选和反应程序的优化。结果表明,250对SSR引物中有223对引物扩增成功,大部分引物的退火温度为55~57℃,共筛选出125对在耐盐碱品种BJ-299与盐碱敏感品种Tx622B间表现出多态性的SSR引物。 展开更多
关键词 高粱[Sorghum bicolor(L.) Moench] SSR PCR 引物筛选
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高粱光敏色素A基因的克隆、蛋白结构与长短日照诱导表达模式 被引量:1
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作者 王平 王春语 +2 位作者 赵子良 丛玲 陆晓春 《分子植物育种》 CAS CSCD 北大核心 2020年第6期1748-1754,共7页
光敏色素是一类红光/远红光受体,对植物光形态建成、外部形态和生理功能等方面起着重要的调节作用。为明晰光敏色素A在调控高粱光形态建成发育过程中的作用,本研究比较分析光温敏感程度不同的高粱品种Btx623、Rio、Tx430和LH645的PhyA... 光敏色素是一类红光/远红光受体,对植物光形态建成、外部形态和生理功能等方面起着重要的调节作用。为明晰光敏色素A在调控高粱光形态建成发育过程中的作用,本研究比较分析光温敏感程度不同的高粱品种Btx623、Rio、Tx430和LH645的PhyA基因序列和PHYA蛋白的保守结构域,利用qRT-PCR明确长短日照条件处理下PhyA基因的表达模式。结果表明,Btx623、Rio和Tx430之间PhyA基因序列存在非同义突变,LH645在PhyA基因的第7个外显子上插入了5 bp碱基序列;高粱PhyA蛋白中含有1个PAS-2结构域、1个GAF结构域、1个Phytochrome结构域、2个PAS结构域、1个组氨酸激酶A结构域和1个类似组氨酸激酶的ATP激酶结构;遗传进化树分析表明,单子叶和双子叶植物PhyA蛋白明显聚为2大类,高粱PhyA、谷子PhyA和玉米PhyA1、PhyA2聚为一个亚类,相互间亲缘关系较近,与水稻PhyA同源性相对较远;在短日照(SD)条件下各时期PhyA基因转录水平变化显著,Rio PhyA基因转录水平较Btx623和Tx430变化明显,LH645 PhyA基因转录变化不显著,在长日照(LD)条件下Rio PhyA基因转录水平低于Btx623和Tx430,LH645 PhyA基因转录水平变化不显著。以上结果表明,PhyA受光周期诱导,推测其在高粱光形态建成中起重要调控作用。本研究为进一步解析PhyA基因功能及其在调控高粱光形态建成发育过程中的作用提供理论基础。 展开更多
关键词 高粱(Sorghum bicolor) 光敏色素A 表达模式 长/短日照处理
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调整产业结构,发展甜高梁酒精燃料产业 被引量:7
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作者 黎大爵 《农业工程学报》 EI CAS CSCD 北大核心 2003年第z1期168-171,共4页
随着"汽车时代"的到来,能耗与日俱增,自1993年起,我国已成为石油净进口国,2001年进口7000多万t,占我国石油加工能力的36%,能源安全已面临挑战.由于汽车尾气排放出大量的污染物,使大气中CO2的含量增加,加剧温室效应和导致酸雨... 随着"汽车时代"的到来,能耗与日俱增,自1993年起,我国已成为石油净进口国,2001年进口7000多万t,占我国石油加工能力的36%,能源安全已面临挑战.由于汽车尾气排放出大量的污染物,使大气中CO2的含量增加,加剧温室效应和导致酸雨的危险,尾气排出的铅,更遗害无穷.甜高粱(Sorghum bicolor(L.)Moench)具有极高的光合速率,故有"高能植物"之称.在我国,甜高粱的单位面积酒精产量远高于玉米、甜菜和甘蔗,更由于甜高梁耐旱、耐涝、耐盐碱,特别适于我国栽培,加之在甜高粱研究与新品种选育这一领域我国居世界领先地位,开发甜高粱酒精燃料的时机已经成熟,它将对我国产业结构的调整、能源安全、环境保护起举足轻重的作用. 展开更多
关键词 甜高粱 SORGHUM bicolor(L.)Moench 酒精燃料 生物能源 环境保护 产业结构调整
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花叶芋(Caladium bicolor)组培的改进及细胞组织学观察 被引量:1
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作者 李乐攻 黎伶俐 郑建国 《植物学报》 CAS CSCD 1992年第S1期34-34,共1页
花叶芋(Calalium bicolor),又名五彩芋,属天南星科,是一种美丽的观叶植物,原是通过块茎繁殖,但其休眠期长,增殖速度慢,因此,利用组培扩大繁殖,以满足市场需要。关于花叶芋的组培,已有不少报道,但对合适的培养基的筛选,分化的途径都还有... 花叶芋(Calalium bicolor),又名五彩芋,属天南星科,是一种美丽的观叶植物,原是通过块茎繁殖,但其休眠期长,增殖速度慢,因此,利用组培扩大繁殖,以满足市场需要。关于花叶芋的组培,已有不少报道,但对合适的培养基的筛选,分化的途径都还有研究的必要。为此,我们以花叶芋叶柄、叶片为材料进行了初步研究现结果如下:1.愈伤组织的诱导和培养基的改进:所用培养基中在 MS 附加2,4-D1ppm,KT 1ppm、 展开更多
关键词 花叶芋 细胞组织学 BICOLOR 观叶植物 块茎繁殖 愈伤组织 五彩芋 继代 增殖速度 满足市场需要
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New taxa of Berberis (Berberidaceae) with greenish flowers from a biodiversity hotspot in Sichuan Province, China 被引量:3
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作者 Xinhua Li Licun Zhang +2 位作者 Wenhui Li Xiaoming Yin Sen Yuan 《Plant Diversity》 SCIE CAS CSCD 北大核心 2017年第2期94-103,共10页
A new species,Berberis viridiflora X.H.Li,and a new variety,Berberis sanguinea Franch.var.viridisepala X.H.Li,L.C.Zhang & W.H.Li are described and illustrated from Baoxing County,a biodiversity hotspot located on ... A new species,Berberis viridiflora X.H.Li,and a new variety,Berberis sanguinea Franch.var.viridisepala X.H.Li,L.C.Zhang & W.H.Li are described and illustrated from Baoxing County,a biodiversity hotspot located on the eastern edge of Hengduan Mountains in Sichuan Province,Southwest China,Both new taxa resemble B.sanguinea Franch.var.sanguinea,but B.viridiflora differs by the greenish flowers,and the petals being truncate,obtuse,or undulate at apex;while B.sanguinea var.viridisepala differs by the greenish or yellowish green flowers.Morphological features of the pollen grains of B.sanguinea and the two new taxa are revealed by scanning election microscope.B.sanguinea var.sanguinea displays obvious similarities with a sympatric congener,Berberis multiovula T.S.Ying in the morphology of flowers,stems and leaves,especially its ovule number varies greatly from 2 to 9,thus,B.multiovula characterized by the5-ovuled ovary is reduced to a synonymy of B.sanguinea var.sanguinea.On the basis of field surveys and study of herbarium specimens,a total of 16 species and varieties of Berberis are recorded from Baoxing County,and a key is provided.Among the 16 taxa,most phenotypic variations in Chinese Berberis can be displayed,including the habit of plants,most morphological variations of stems,branches and leaves,all types of inflorescences,all color types of flowers,and nearly all types of the shape and color of fruits.Baoxing County and its adjacent Sichuan Giant Panda Sanctuaries possibly constitute an active diversification center of Berberis in eastern Hengduan Mountains of China. 展开更多
关键词 BERBERIS Greenish flowers Purplish red flowers bicolored flowers Ovule number Baoxing County
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Effects of Salt Stress on Root Vitality of Limonium bicolor (Bunge) Kuntze 被引量:2
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作者 林莺 范海 《Agricultural Science & Technology》 CAS 2013年第6期832-834,910,共4页
[Objective] This study aimed to investigate the effects of different salt concentrations on the root vitality of Limonium bicolor (Bunge) Kuntze. [Method] Limonium bicolor (Bunge) Kuntze was treated with 0, 100, 2... [Objective] This study aimed to investigate the effects of different salt concentrations on the root vitality of Limonium bicolor (Bunge) Kuntze. [Method] Limonium bicolor (Bunge) Kuntze was treated with 0, 100, 200 and 400 mmol/L NaCl, respectively. After two weeks, root vitality, biomass and other physiological indicators were measured. [Result] Salt stress had significant influences on the growth of roots. Root vitality of Limonium bicolor increased firstly and reduced gradually with the increase of salt concentration. [Conclusion] The results indicate that Limonium bicolor has certain salt tolerance ability at low salt concentrations; under relatively high salt concentrations, Limonium bicolor roots can maintain high vitality; however, with the continuous increase of salt concentration, Limonium bicolor roots are damaged, with decreasing vitality. 展开更多
关键词 Limonium bicolor (Bunge) Kuntze Salt stress Root vitality
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花叶芋和观音莲抗寒性的初步研究 被引量:7
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作者 刘伟坚 黄映鲜 梁科 《大众科技》 2005年第7期127-128,共2页
在不同的低温胁迫下,对花叶芋(Caladiumbicolor)、观音莲(Alocasiaamazonica)外部形态变化、叶绿素含量进行研究,以确定其抗寒性。结果表明:在10℃时花叶芋和观音莲的伤害度分别为15.51%,27.82%,8℃分别达到26.96%和49.33%,5℃达到45.64... 在不同的低温胁迫下,对花叶芋(Caladiumbicolor)、观音莲(Alocasiaamazonica)外部形态变化、叶绿素含量进行研究,以确定其抗寒性。结果表明:在10℃时花叶芋和观音莲的伤害度分别为15.51%,27.82%,8℃分别达到26.96%和49.33%,5℃达到45.64%和61.35%.观音莲将近死亡。低温下,叶绿素合成受到抑制,含量降低,且观音莲的下降大于花叶芋,所以也说明了观音莲的抗寒性比花叶芋差。 展开更多
关键词 花叶芋(Caladium bicolor) 观音莲(Alocasia amazonica) 荫生植物 抗寒性 叶绿素含量 伤害度
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Exploration and Innovation of Distant Hybridization Germplasm of Spartina,Soughum bicolor and Oryza sativa
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作者 戴晖 陈启康 +3 位作者 田曾元 顾拥建 沙文锋 朱娟 《Agricultural Science & Technology》 CAS 2012年第10期2065-2069,共5页
[Objective] This study aimed to explore the germplasm resources of salt-tolerant Spartina,sweet-stalk Soughum bicolor and high-yield Oryza sativa for innovation of new germplasms of salt-tolerant and sweet-stalk Oryza... [Objective] This study aimed to explore the germplasm resources of salt-tolerant Spartina,sweet-stalk Soughum bicolor and high-yield Oryza sativa for innovation of new germplasms of salt-tolerant and sweet-stalk Oryza sativa with dual purpose of food and forage in sea beach.[Method] From 2009 to 2012,researches on distant hybridization of Spartina,Soughum bicolor and Oryza sativa had been carried out,and the RAPD molecular identification of obtained distant hybridization materials had been conducted.[Result] Experimental evidences of hybrid seed setting,transplanting and economic characters of hybrid materials were obtained;among the 14 hybrid materials for RAPD analysis,5 materials shared common bands with Spartina and Soughum bicolor parents,which were absent in Oryza sativa parent,indicating that the genetic component of Spartina and Soughum bicolor parents has been integrated into the 5 distant hybrids.[Conclusion] This study has important scientific significance in recourse utilization,agricultural efficiency improvement,food security,and cultivation strategy of China,showing broad application prospects. 展开更多
关键词 Spartina × Soughum bicolor × Oryza sativa Distant hybridization Germplasm innovation
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高粱WRKY家族成员鉴定及生物信息学分析 被引量:8
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作者 赵兴奎 范昕琦 +3 位作者 聂萌恩 王海燕 柳青山 段永红 《分子植物育种》 CAS CSCD 北大核心 2020年第13期4170-4181,共12页
WRKY转录因子是调控植物生长发育的重要转录因子,本研究利用高粱基因组数据库(v3.1.1),通过生物信息学分析鉴定出97个高粱WRKY基因家族成员,其CDS序列长度为423~4965 bp,编码氨基酸长度为141~1655 aa。染色体定位发现97个基因不均匀地... WRKY转录因子是调控植物生长发育的重要转录因子,本研究利用高粱基因组数据库(v3.1.1),通过生物信息学分析鉴定出97个高粱WRKY基因家族成员,其CDS序列长度为423~4965 bp,编码氨基酸长度为141~1655 aa。染色体定位发现97个基因不均匀地分布于10条染色体上,其中3号染色体上数目最多,有23个WRKY基因,6号染色体最少,只有5个WRKY基因。根据WRKY保守结构域和锌指结构的特点对97个转录因子进行分类,可将它们分为Ⅰ、Ⅱ和Ⅲ三大类,分别有11个、70个和16个成员。另外对WRKY蛋白保守基序分析发现WRKY结构域有3个保守基序组成,各组成员的保守基序基本一致。部分蛋白的WRKY保守域和锌指结构具有多样性,分析发现部分高粱WRKY蛋白的WRKYGQK结构域中‘Q’突变为‘E’、‘S’和‘K’。系统进化关系显示高粱Ⅱ、Ⅲ类WRKY转录因子类聚在Ⅰ的下级分支上,表示Ⅱ和Ⅲ类可能是由Ⅰ类进化而来的。 展开更多
关键词 高粱(Sorghum bicolor) WRKY转录因子 系统进化 生物信息学分析
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