目的:探讨中药复方白香丹胶囊(BXD)对经前期综合征(premenstrual syndrome,PMS)肝气逆证模型大鼠海马中γ-氨基丁酸A受体(GABAAR)β2亚基蛋白分布模式及表达水平的影响。方法:阴道涂片镜检法和旷场实验筛选合适大鼠,随机分为正常组、肝...目的:探讨中药复方白香丹胶囊(BXD)对经前期综合征(premenstrual syndrome,PMS)肝气逆证模型大鼠海马中γ-氨基丁酸A受体(GABAAR)β2亚基蛋白分布模式及表达水平的影响。方法:阴道涂片镜检法和旷场实验筛选合适大鼠,随机分为正常组、肝逆组和肝逆给药组(BXD给药剂量10 g.kg-1.d-1,给药5 d);电刺激法复制PMS肝气逆证模型,同时采用宏观行为学观察和旷场实验来评价模型;免疫荧光技术和蛋白印迹技术(Western Blot)测定大鼠海马神经元中GABAA R β2亚基蛋白分布模式及表达水平。结果:与正常组相比,肝逆组大鼠旷场实验得分明显增加,海马神经元GABAAR较集中分布于组织边缘,β2亚基蛋白表达水平显著升高(P<0.01);而造模同时给予BXD后,大鼠旷场实验得分明显减少,海马神经元GABAA R分布模式无明显变化,而β2亚基蛋白表达水平显著降低(P<0.05)。结论:大鼠海马神经元GABAA R β2亚基蛋白表达水平上调可能是与PMS肝气逆证密切相关的重要微观机制之一;中药复方BXD可对GABAA R β2亚基蛋白表达水平变化起到纠正作用。展开更多
This work successfully used model rats with Pre-Menstrual Syndrome (PMS) liver-qi invasion in the early de-velopment days to detect the Metabolic and Behavioral Patterns and their reversal by a Chinese traditional for...This work successfully used model rats with Pre-Menstrual Syndrome (PMS) liver-qi invasion in the early de-velopment days to detect the Metabolic and Behavioral Patterns and their reversal by a Chinese traditional for-mula. Our aim is to verify the reliability of PMS liver-qi invasion rat model and explore some micro- mecha-nism of the syndrome of the liver failing to maintain the normal flow of qi. 30 rats with estrous cycles not in accepting time were selected and divided randomly into three groups: the normal control group, PMS liver-qi invasion model group and PMS liver-qi invasion medication-administered group. Emotional stimulation and multiple factors combination were used to prepare the PMS liver-qi invasion model. Baixiangdan Capsules (a Chinese traditional formula) were administered to rats to interfere with the PMS liver-qi invasion mode. Open-field test was used to explore behavioral aspects of the model. Urine samples, from the three groups, were collected and analyzed with UPLC-Q-TOF method to detect changes in metabolites related to liver func-tions. In the open-field experiment, the crossing scores, rearing scores and open-field experiment total scores of rats in the PMS liver-qi invasion model group increased remarkably (P < 0.05) compared with the scores of the normal control group, the tendency was retrieved remarkably after medications (P < 0.05). Metabolic finger-prints between the PMS liver-qi invasion model group and the normal control group had also distinguished changes through principal component analysis, and an evident restoration trend occurred after Baixiangdan Capsules administration. Taken together, behavioral and metabolic patterns can differentiate the PMS liver-qi invasion rat models from the normal rats. Our results identified potential biological markers that might reflect metabolic pathologies associated with PMS liver-qi invasion.展开更多
文摘目的:探讨中药复方白香丹胶囊(BXD)对经前期综合征(premenstrual syndrome,PMS)肝气逆证模型大鼠海马中γ-氨基丁酸A受体(GABAAR)β2亚基蛋白分布模式及表达水平的影响。方法:阴道涂片镜检法和旷场实验筛选合适大鼠,随机分为正常组、肝逆组和肝逆给药组(BXD给药剂量10 g.kg-1.d-1,给药5 d);电刺激法复制PMS肝气逆证模型,同时采用宏观行为学观察和旷场实验来评价模型;免疫荧光技术和蛋白印迹技术(Western Blot)测定大鼠海马神经元中GABAA R β2亚基蛋白分布模式及表达水平。结果:与正常组相比,肝逆组大鼠旷场实验得分明显增加,海马神经元GABAAR较集中分布于组织边缘,β2亚基蛋白表达水平显著升高(P<0.01);而造模同时给予BXD后,大鼠旷场实验得分明显减少,海马神经元GABAA R分布模式无明显变化,而β2亚基蛋白表达水平显著降低(P<0.05)。结论:大鼠海马神经元GABAA R β2亚基蛋白表达水平上调可能是与PMS肝气逆证密切相关的重要微观机制之一;中药复方BXD可对GABAA R β2亚基蛋白表达水平变化起到纠正作用。
文摘This work successfully used model rats with Pre-Menstrual Syndrome (PMS) liver-qi invasion in the early de-velopment days to detect the Metabolic and Behavioral Patterns and their reversal by a Chinese traditional for-mula. Our aim is to verify the reliability of PMS liver-qi invasion rat model and explore some micro- mecha-nism of the syndrome of the liver failing to maintain the normal flow of qi. 30 rats with estrous cycles not in accepting time were selected and divided randomly into three groups: the normal control group, PMS liver-qi invasion model group and PMS liver-qi invasion medication-administered group. Emotional stimulation and multiple factors combination were used to prepare the PMS liver-qi invasion model. Baixiangdan Capsules (a Chinese traditional formula) were administered to rats to interfere with the PMS liver-qi invasion mode. Open-field test was used to explore behavioral aspects of the model. Urine samples, from the three groups, were collected and analyzed with UPLC-Q-TOF method to detect changes in metabolites related to liver func-tions. In the open-field experiment, the crossing scores, rearing scores and open-field experiment total scores of rats in the PMS liver-qi invasion model group increased remarkably (P < 0.05) compared with the scores of the normal control group, the tendency was retrieved remarkably after medications (P < 0.05). Metabolic finger-prints between the PMS liver-qi invasion model group and the normal control group had also distinguished changes through principal component analysis, and an evident restoration trend occurred after Baixiangdan Capsules administration. Taken together, behavioral and metabolic patterns can differentiate the PMS liver-qi invasion rat models from the normal rats. Our results identified potential biological markers that might reflect metabolic pathologies associated with PMS liver-qi invasion.
