Objective:To investigate the potential efficacy and safety of Lutai Danshen Baishao granules(LDBG)for treating female melasma associated with kidney deficiency and blood stasis patterns.Methods:A randomized,double-bli...Objective:To investigate the potential efficacy and safety of Lutai Danshen Baishao granules(LDBG)for treating female melasma associated with kidney deficiency and blood stasis patterns.Methods:A randomized,double-blind,placebo-controlled trial was conducted at the Third Central Hospital of Tianjin,China from March to December 2023.A total of 110 female patients with melasma linked to kidney deficiency and blood stasis were enrolled and treated with either LDBG or a placebo twice daily for 60 days.Efficacy was assessed through measures such as the total melasma area,reduced melasma area,reduction rate of melasma area,melasma color score,Melasma Area and Severity Index(MASI)score,and traditional Chinese medicine(TCM)symptom score scale.Safety assessments included routine blood and biochemical tests.Results:Participants in both groups were aged 52-63 years,with no significant differences.After the 2-month intervention,the total melasma area decreased in both groups;however,a greater reduction was observed in the test group[462.50 mm^(2)(12.81%)vs.100.00 mm2(3.11%),P<.001].Moreover,LDBG treatment significantly reduced the MASI and melasma color scores in the test group(P<.05).The total TCM symptom evaluation score significantly decreased(test group:6.00 vs.placebo group:7.00,P=.001),with significant relief in symptoms such as improvement in dark lips,nails,and waist soreness in the test group,compared with that in the placebo group(P<.05).Within-group comparisons revealed that TCM syndrome was significantly alleviated in the test group(P<.05).Conclusion:LDBG intervention shows promising effectiveness in reducing female melasma and alleviating TCM syndromes.展开更多
OBJECTIVE:To elucidate the potential molecular mechanisms of Baishao(Radix Paeoniae Alba)(APR)and Gancao(Radix Glycyrrhizae)(GR)in the treatment of major depressive disorder(MDD).METHODS:Based on the network pharmacol...OBJECTIVE:To elucidate the potential molecular mechanisms of Baishao(Radix Paeoniae Alba)(APR)and Gancao(Radix Glycyrrhizae)(GR)in the treatment of major depressive disorder(MDD).METHODS:Based on the network pharmacology strategy,the therapeutic targets of APR-GR for MDD are predicted,differentially expressed genes from the Integrated Gene Expression database for MDD patients.Topological networks are constructed,Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathways are enriched,their pharmacological potential molecular mechanisms are discussed,and molecular docking analysis is performed to further motivate compositional and target interactions.Finally,the CUMS mouse model is used for validation.RESULTS:Based on the pharmacological network analysis,17 candidate genes were identified,including muscarinic acetylcholine receptor M1(CHRM1),muscarinic acetylcholine receptor M2(CHRM2),β2-adrenergic receptor(ADRB2),adrenergicα1A receptor(ADRA1A)and 5-hydroxytryptamine transfer protein(SLC6A4),etc.which are primarily involved in reactive oxygen species metabolism,neural response,oxidative stress response and other biological processes.Further analysis revealed that these targets are closely related to Ca^(2+),cyclic adenosine monophosphate,etc.,and exhibit optimal binding sites after molecular docking.Finally,in vivo experiments were performed and it was found that APR-GR significantly improved depression-like behavior and hippocampal impairment in mouse models,increasing brain levels of 5-hydroxytryptamine,dopamine and norepinephrine and decreasing serum levels of corticotropin releasing hormone,corticosterone and adreno cortico tropic hormone,while upregulating the expression of CHRM1,CHRM2 and ADRA1A in the hippocampus and downregulating the expression of SLC6A4 and ADRB2.CNCLUSION:This research sheds light on the potential molecular mechanism of APR-GR to improve MDD.展开更多
Objective To evaluate the difference between Baishao(Paeoniae Radix Alba,PRA)and Chishao(Paeoniae Radix Rubra,PRR)from different regions based on the characteristic spectra of amino acids(AAs).Methods Fingerprints of ...Objective To evaluate the difference between Baishao(Paeoniae Radix Alba,PRA)and Chishao(Paeoniae Radix Rubra,PRR)from different regions based on the characteristic spectra of amino acids(AAs).Methods Fingerprints of the 21 standard AAs were established using O-phthalaldehyde-9-fluorenylmethyl chloroformate(OPA-FMOC)pre-column derivation method.The AA components in PRA and PRR were characterized qualitatively and quantitatively,and different AAs were screened using pattern recognition technology.Results Twelve AAs were identified in both PRA and PRR.Meanwhile,aspartic acid,glutamic acid,arginine,alanine,and gamma-aminobutyric acid were screened as characteristic components,and their concentrations could effectively distinguish PRA from PRR.Conclusion The established characterization method,which is based on the characteristic spectra of AAs,is accurate,efficient,and sensitive and can effectively distinguish between PRA and PRR from different producing areas,thus providing a reference for the overall characterization and evaluation of Chinese medicinal materials.展开更多
目的:基于网络药理学方法和分子对接技术探讨白芍-熟地黄-当归-天麻药物组合治疗帕金森病(Parkinson's disease,PD)的作用机制。方法:通过HERB数据库筛选白芍-熟地黄-当归-天麻主要活性成分和作用靶点,利用GeneCards、DrugBank、Dis...目的:基于网络药理学方法和分子对接技术探讨白芍-熟地黄-当归-天麻药物组合治疗帕金森病(Parkinson's disease,PD)的作用机制。方法:通过HERB数据库筛选白芍-熟地黄-当归-天麻主要活性成分和作用靶点,利用GeneCards、DrugBank、DisGeNET、OMIM数据库检索PD相关靶点,取两者交集,并通过Cytoscape软件绘制“药物-成分-交集靶点-疾病”网络图筛选活性成分。基于STRING数据库构建蛋白质相互作用(protein-protein interaction,PPI)网络。筛选关键靶点,利用DAVID数据库进行基因本体(gene ontology,GO)功能和京都基因与基因组百科全书(kyoto encyclopedia of genes and genomes,KEGG)通路富集分析,对活性成分与关键靶点进行分子对接。结果:获得白芍59个、熟地黄21个、当归125个、天麻17个主要活性成分,对应1288个作用靶点,与PD交集靶点390个,筛选出4-乙基苯甲醛、顺式-异丁香酚、野菰酸、叶下珠脂素、4-乙基间苯二酚等为核心活性成分,PPI网络拓扑分析提示HSP90AA1、Akt1、JUN、CTNNB1、ESR1等为关键靶点。白芍-熟地黄-当归-天麻治疗PD的信号通路主要富集于脂质和动脉粥样硬化通路、癌症通路、神经活性配体-受体相互作用通路、环腺苷酸信号通路、流体剪切应力与动脉粥样硬化通路、糖尿病并发症中的晚期糖基化终末产物-晚期糖基化终末产物受体(advanced glycation end products-receptor for advanced glycation end products,AGE-RAGE)信号通路、钙信号通路、化学致癌-受体激活通路等。分子对接显示核心成分4-乙基苯甲醛、顺式-异丁香酚、野菰酸、叶下珠脂素、4-乙基间苯二酚等与核心靶点HSP90AA1、JUN、CTNNB1、ESR1等对接程度良好。结论:白芍-熟地黄-当归-天麻中4-乙基苯甲醛、顺式-异丁香酚、野菰酸、叶下珠脂素、4-乙基间苯二酚等有效成分可能通过作用于HSP90AA1、JUN、CTNNB1、ESR1等靶点调节多条信号通路,达到治疗PD的作用。展开更多
基金funded by the National Key Research and Development Plan of the Traditional Chinese Medicine Modernization Research Key Project(2018YFC1706800).
文摘Objective:To investigate the potential efficacy and safety of Lutai Danshen Baishao granules(LDBG)for treating female melasma associated with kidney deficiency and blood stasis patterns.Methods:A randomized,double-blind,placebo-controlled trial was conducted at the Third Central Hospital of Tianjin,China from March to December 2023.A total of 110 female patients with melasma linked to kidney deficiency and blood stasis were enrolled and treated with either LDBG or a placebo twice daily for 60 days.Efficacy was assessed through measures such as the total melasma area,reduced melasma area,reduction rate of melasma area,melasma color score,Melasma Area and Severity Index(MASI)score,and traditional Chinese medicine(TCM)symptom score scale.Safety assessments included routine blood and biochemical tests.Results:Participants in both groups were aged 52-63 years,with no significant differences.After the 2-month intervention,the total melasma area decreased in both groups;however,a greater reduction was observed in the test group[462.50 mm^(2)(12.81%)vs.100.00 mm2(3.11%),P<.001].Moreover,LDBG treatment significantly reduced the MASI and melasma color scores in the test group(P<.05).The total TCM symptom evaluation score significantly decreased(test group:6.00 vs.placebo group:7.00,P=.001),with significant relief in symptoms such as improvement in dark lips,nails,and waist soreness in the test group,compared with that in the placebo group(P<.05).Within-group comparisons revealed that TCM syndrome was significantly alleviated in the test group(P<.05).Conclusion:LDBG intervention shows promising effectiveness in reducing female melasma and alleviating TCM syndromes.
基金Supported by Jilin Provincial Department of Science and Technology project:Exploring the Material Basis and Action Pathways of Baihu Tang's Antipyretic Effect based on Omics Technology(20240602036RC)。
文摘OBJECTIVE:To elucidate the potential molecular mechanisms of Baishao(Radix Paeoniae Alba)(APR)and Gancao(Radix Glycyrrhizae)(GR)in the treatment of major depressive disorder(MDD).METHODS:Based on the network pharmacology strategy,the therapeutic targets of APR-GR for MDD are predicted,differentially expressed genes from the Integrated Gene Expression database for MDD patients.Topological networks are constructed,Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathways are enriched,their pharmacological potential molecular mechanisms are discussed,and molecular docking analysis is performed to further motivate compositional and target interactions.Finally,the CUMS mouse model is used for validation.RESULTS:Based on the pharmacological network analysis,17 candidate genes were identified,including muscarinic acetylcholine receptor M1(CHRM1),muscarinic acetylcholine receptor M2(CHRM2),β2-adrenergic receptor(ADRB2),adrenergicα1A receptor(ADRA1A)and 5-hydroxytryptamine transfer protein(SLC6A4),etc.which are primarily involved in reactive oxygen species metabolism,neural response,oxidative stress response and other biological processes.Further analysis revealed that these targets are closely related to Ca^(2+),cyclic adenosine monophosphate,etc.,and exhibit optimal binding sites after molecular docking.Finally,in vivo experiments were performed and it was found that APR-GR significantly improved depression-like behavior and hippocampal impairment in mouse models,increasing brain levels of 5-hydroxytryptamine,dopamine and norepinephrine and decreasing serum levels of corticotropin releasing hormone,corticosterone and adreno cortico tropic hormone,while upregulating the expression of CHRM1,CHRM2 and ADRA1A in the hippocampus and downregulating the expression of SLC6A4 and ADRB2.CNCLUSION:This research sheds light on the potential molecular mechanism of APR-GR to improve MDD.
基金We thank for the funding support from the Guangdong Provincial Key Laboratory of Chinese Medicine for Prevention and Treatment of Refractory Chronic Diseases(No.2018B030322012).
文摘Objective To evaluate the difference between Baishao(Paeoniae Radix Alba,PRA)and Chishao(Paeoniae Radix Rubra,PRR)from different regions based on the characteristic spectra of amino acids(AAs).Methods Fingerprints of the 21 standard AAs were established using O-phthalaldehyde-9-fluorenylmethyl chloroformate(OPA-FMOC)pre-column derivation method.The AA components in PRA and PRR were characterized qualitatively and quantitatively,and different AAs were screened using pattern recognition technology.Results Twelve AAs were identified in both PRA and PRR.Meanwhile,aspartic acid,glutamic acid,arginine,alanine,and gamma-aminobutyric acid were screened as characteristic components,and their concentrations could effectively distinguish PRA from PRR.Conclusion The established characterization method,which is based on the characteristic spectra of AAs,is accurate,efficient,and sensitive and can effectively distinguish between PRA and PRR from different producing areas,thus providing a reference for the overall characterization and evaluation of Chinese medicinal materials.
文摘目的:基于网络药理学方法和分子对接技术探讨白芍-熟地黄-当归-天麻药物组合治疗帕金森病(Parkinson's disease,PD)的作用机制。方法:通过HERB数据库筛选白芍-熟地黄-当归-天麻主要活性成分和作用靶点,利用GeneCards、DrugBank、DisGeNET、OMIM数据库检索PD相关靶点,取两者交集,并通过Cytoscape软件绘制“药物-成分-交集靶点-疾病”网络图筛选活性成分。基于STRING数据库构建蛋白质相互作用(protein-protein interaction,PPI)网络。筛选关键靶点,利用DAVID数据库进行基因本体(gene ontology,GO)功能和京都基因与基因组百科全书(kyoto encyclopedia of genes and genomes,KEGG)通路富集分析,对活性成分与关键靶点进行分子对接。结果:获得白芍59个、熟地黄21个、当归125个、天麻17个主要活性成分,对应1288个作用靶点,与PD交集靶点390个,筛选出4-乙基苯甲醛、顺式-异丁香酚、野菰酸、叶下珠脂素、4-乙基间苯二酚等为核心活性成分,PPI网络拓扑分析提示HSP90AA1、Akt1、JUN、CTNNB1、ESR1等为关键靶点。白芍-熟地黄-当归-天麻治疗PD的信号通路主要富集于脂质和动脉粥样硬化通路、癌症通路、神经活性配体-受体相互作用通路、环腺苷酸信号通路、流体剪切应力与动脉粥样硬化通路、糖尿病并发症中的晚期糖基化终末产物-晚期糖基化终末产物受体(advanced glycation end products-receptor for advanced glycation end products,AGE-RAGE)信号通路、钙信号通路、化学致癌-受体激活通路等。分子对接显示核心成分4-乙基苯甲醛、顺式-异丁香酚、野菰酸、叶下珠脂素、4-乙基间苯二酚等与核心靶点HSP90AA1、JUN、CTNNB1、ESR1等对接程度良好。结论:白芍-熟地黄-当归-天麻中4-乙基苯甲醛、顺式-异丁香酚、野菰酸、叶下珠脂素、4-乙基间苯二酚等有效成分可能通过作用于HSP90AA1、JUN、CTNNB1、ESR1等靶点调节多条信号通路,达到治疗PD的作用。
