OBJECTIVE:To investigate the underlying mechanism of Bailian (Radix Ampelopsis Japonicae,BL) extract action on colorectal cancer (CRC).METHODS:We explored the involvement of β-catenin signaling on the anti-CRC effect...OBJECTIVE:To investigate the underlying mechanism of Bailian (Radix Ampelopsis Japonicae,BL) extract action on colorectal cancer (CRC).METHODS:We explored the involvement of β-catenin signaling on the anti-CRC effects of an BL ethanolic extract (BLE) in cell models by using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay,immunofluorescent staining,luciferase assay,Western blot analysis and real-time quantitative polymerase chain reaction analysis.Anti-CRC compounds were quantified by high performance liquid chromatography.RESULTS:The contents of gallic acid,catechin,and epicatechin in the BLE were 0.23,1.25,and 0.18 g/kg,respectively.BLE-mediated cytotoxic and apoptotic effects were accompanied by lowered β-catenin/Tcf transcriptional activity,reduced β-catenin nuclear localization,and downregulated protein and mRNA levels of both β-catenin and molecules regulated by β-catenin.CONCLUSION:The mechanism underpinning the anti-CRC effects of BLE may involve inhibition of β-catenin signaling.Further studies are necessary to establish the role of β-catenin signaling in the action of BLE-mediated anti-CRC effects.展开更多
In order to extend the forecasting period of flood and improve the accuracy of flood forecasting,this paper took Bailian River Reservoir which located in Huanggang City of Hubei Province as an example and carried out ...In order to extend the forecasting period of flood and improve the accuracy of flood forecasting,this paper took Bailian River Reservoir which located in Huanggang City of Hubei Province as an example and carried out basin flood simulation and forecasting by coupling the quantitative precipitation forecasting products of numerical forecast operation model of Institute of Heavy Rain in Wuhan(WRF)and the European Center for Medium-range Weather Forecasts(ECMWF)with the three water sources Xin an River model.The experimental results showed that the spatiotemporal distribution of rainfall predicted by EC is closer to the actual situation compared to WRF;the efficiency coefficient and peak time difference of EC used for flood forecasting are comparable to WRF,but the average relative error of flood peaks is about 14%smaller than WRF.Overall,the precipitation forecasting products of the two numerical models can be used for flood forecasting in the Bailian River basin.Some forecasting indicators have certain reference value,and there is still significant room for improvement in the forecasting effects of the two models.展开更多
基金Supported by Food and Health Bureau of Hong Kong:Elucidating the Involvement of IL-17-IL-6-STAT3 Axis in the anti-melanoma Effects of a Herbal Formula Comprising Flos Sophorae and Flos Lonicerae(No.HMRF14150571)Science,Technology and Innovation Commission of Shenzhen:anti-colorectal Cancer Effects of Bailian(Radix Ampelopsis Japonicae)extract(No.JCYJ20140807091945050)+3 种基金Exploring the Mechanisms for the anti-rheumatoid Rrthritis Effects of Chinese Medicinal Herb Based on Targeting the TLR4 Signaling Pathway(No.JCYJ20160229210327924)The Research Grants Council of Hong Kong:Elucidating the role of TLR4/STAT3 Signaling in the Antimelanoma Effects of Atractylenolide Ⅱ(No.12125116)The National Natural Science Foundation of China:Exploring the let-7/IGF1R-related Mechanism of Action for the Anti-melanoma Effects of a Herbal formula Huai Hua Jin Yin Jiu(No.81673649)Guangdong Natural Science Foundation:Evaluating the Involvement of miR-34b in the Anti-melanoma Action of Si-Jun-Zi-Tang(No.2016A030313007)
文摘OBJECTIVE:To investigate the underlying mechanism of Bailian (Radix Ampelopsis Japonicae,BL) extract action on colorectal cancer (CRC).METHODS:We explored the involvement of β-catenin signaling on the anti-CRC effects of an BL ethanolic extract (BLE) in cell models by using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay,immunofluorescent staining,luciferase assay,Western blot analysis and real-time quantitative polymerase chain reaction analysis.Anti-CRC compounds were quantified by high performance liquid chromatography.RESULTS:The contents of gallic acid,catechin,and epicatechin in the BLE were 0.23,1.25,and 0.18 g/kg,respectively.BLE-mediated cytotoxic and apoptotic effects were accompanied by lowered β-catenin/Tcf transcriptional activity,reduced β-catenin nuclear localization,and downregulated protein and mRNA levels of both β-catenin and molecules regulated by β-catenin.CONCLUSION:The mechanism underpinning the anti-CRC effects of BLE may involve inhibition of β-catenin signaling.Further studies are necessary to establish the role of β-catenin signaling in the action of BLE-mediated anti-CRC effects.
基金Supported by Open Project Fund of China Meteorological Administration Basin Heavy Rainfall Key Laboratory(2023BHR-Y26)Innovation Project Fund of Wuhan Metropolitan Area Meteorological Joint Science and Technology(WHCSQY202305)+1 种基金Innovation and Development Special Project of China Meteorological Administration(CXFZ2022J019)Project of Huanggang Meteorological Bureau's Scientific Research(2022Y02).
文摘In order to extend the forecasting period of flood and improve the accuracy of flood forecasting,this paper took Bailian River Reservoir which located in Huanggang City of Hubei Province as an example and carried out basin flood simulation and forecasting by coupling the quantitative precipitation forecasting products of numerical forecast operation model of Institute of Heavy Rain in Wuhan(WRF)and the European Center for Medium-range Weather Forecasts(ECMWF)with the three water sources Xin an River model.The experimental results showed that the spatiotemporal distribution of rainfall predicted by EC is closer to the actual situation compared to WRF;the efficiency coefficient and peak time difference of EC used for flood forecasting are comparable to WRF,but the average relative error of flood peaks is about 14%smaller than WRF.Overall,the precipitation forecasting products of the two numerical models can be used for flood forecasting in the Bailian River basin.Some forecasting indicators have certain reference value,and there is still significant room for improvement in the forecasting effects of the two models.
