This study aimed to explore an analysis method for baicalin in Scutellaria baicalensis Georgi and make a reference for the analysis of the medicinal value of S. baicalensis cultivated in Jizhou District. The content o...This study aimed to explore an analysis method for baicalin in Scutellaria baicalensis Georgi and make a reference for the analysis of the medicinal value of S. baicalensis cultivated in Jizhou District. The content of baicalin in S. baicalensis cultivated in Jizhou District was determined using high-performance liquid chromatography( HPLC) to judge whether it met the requirements of Chinese Pharmacopoeia; and according to the requirements of the Chinese Pharmacopoeia of 2015 edition,the contents of heavy metals were determined by ICP-MS. The results showed that the contents of baicalin,Cr,Cu,As,Cd and Pb in S. baicalensis planted in Jizhou District were 14. 625 05%,11. 360,12. 830,0. 062 315,0. 025 715 and 0. 176 65 μg/g,respectively. The content of baicalin in S. baicalensis planted in Jizhou District was much higher than the standard( ≥ 9. 0%) of Chinese Pharmacopoeia of 2015 edition,and the contents of heavy metals were also below the limit( ≤20/1000 000) required by Chinese Pharmacopoeia of 2015 edition.展开更多
目的:建立高效液相色谱法同时测定三黄微丸中盐酸小檗碱、黄芩苷和大黄素的含量。方法:采用Supersil AQ C_(18)(4.6mm×250mm,5μm)色谱柱,采用乙腈-0.25mol/L磷酸缓冲液梯度洗脱,流速为1.0mL/min,检测波长284nm,柱温30℃,进样量5μ...目的:建立高效液相色谱法同时测定三黄微丸中盐酸小檗碱、黄芩苷和大黄素的含量。方法:采用Supersil AQ C_(18)(4.6mm×250mm,5μm)色谱柱,采用乙腈-0.25mol/L磷酸缓冲液梯度洗脱,流速为1.0mL/min,检测波长284nm,柱温30℃,进样量5μL。结果:盐酸小檗碱、黄芩苷和大黄素在质量浓度范围内线性关系良好,各有效成分相关系数均为r^(2)>0.999,平均回收率分别为99.45%、99.10%和98.80%,RSD均小于2.0%(n=6)。三黄微丸中有效成分的平均含量为0.0801~0.6457mg·g^(-1)(n=3)。结论:本方法简便、准确,适用于三黄微丸的质量控制。展开更多
基金Supported by Tianjin Science and Technology Plan Project(16PTZSTG-00020)Talents Project of Tianjin Agricultural University.Tianjin Science and Technology Plan Project(17JCYBJC29800)Various Talent Funding Project of Tianjin Agricultural University(J01009030702)
文摘This study aimed to explore an analysis method for baicalin in Scutellaria baicalensis Georgi and make a reference for the analysis of the medicinal value of S. baicalensis cultivated in Jizhou District. The content of baicalin in S. baicalensis cultivated in Jizhou District was determined using high-performance liquid chromatography( HPLC) to judge whether it met the requirements of Chinese Pharmacopoeia; and according to the requirements of the Chinese Pharmacopoeia of 2015 edition,the contents of heavy metals were determined by ICP-MS. The results showed that the contents of baicalin,Cr,Cu,As,Cd and Pb in S. baicalensis planted in Jizhou District were 14. 625 05%,11. 360,12. 830,0. 062 315,0. 025 715 and 0. 176 65 μg/g,respectively. The content of baicalin in S. baicalensis planted in Jizhou District was much higher than the standard( ≥ 9. 0%) of Chinese Pharmacopoeia of 2015 edition,and the contents of heavy metals were also below the limit( ≤20/1000 000) required by Chinese Pharmacopoeia of 2015 edition.
文摘目的:建立高效液相色谱法同时测定三黄微丸中盐酸小檗碱、黄芩苷和大黄素的含量。方法:采用Supersil AQ C_(18)(4.6mm×250mm,5μm)色谱柱,采用乙腈-0.25mol/L磷酸缓冲液梯度洗脱,流速为1.0mL/min,检测波长284nm,柱温30℃,进样量5μL。结果:盐酸小檗碱、黄芩苷和大黄素在质量浓度范围内线性关系良好,各有效成分相关系数均为r^(2)>0.999,平均回收率分别为99.45%、99.10%和98.80%,RSD均小于2.0%(n=6)。三黄微丸中有效成分的平均含量为0.0801~0.6457mg·g^(-1)(n=3)。结论:本方法简便、准确,适用于三黄微丸的质量控制。
