Objective： The aim of the study was to investigate inhibitory effects of breast-cancer metastasis suppressor 1 protein （BRMS1） on primary tumor growth and metastasis of human gastric cancer （GC） cells in nude mic...Objective： The aim of the study was to investigate inhibitory effects of breast-cancer metastasis suppressor 1 protein （BRMS1） on primary tumor growth and metastasis of human gastric cancer （GC） cells in nude mice. Methods： We compared the expression of BRMS1 in the primary gastric tumor and metastatic gastric tumor by immunohistochemistry. Expression of BRMS1 also was detected in the GC cells by RT-PCR and Western blot. Three groups of cultured human GC cell line SGC-7901, were maintained： transfected cells with pcDNA3.1（-）B/myc-BRMS1; negative control cells with pcD- NA3.1/myc-his（-）B; and blank control ceils without any transfection. Histologically intact samples of the cells, maintained by passage in the subcutis of nude mice, were transplanted orthotopically into stomach walls of nude mice to establish a nude mouse model of human gastric carcinoma. Their primary tumor growth and metastasis were then observed. Results： The expression of BRMS1 was markedly stronger in the primary gastric tumor compared with metastatic gastric tumor. We also detected BRMS1 gene and protein in the gastric cancer cell tines. Numbers of metastasis tumors significantly differed among mice infected with transfected cells, with negative controls and with blank controls （4.38 ± 0.60, 7.75 ± 0.59, and 7.63± 0.65, respectively; P 〈 0.05）. However, there were no significant differences in the size of orthotopic tumors among mice infected with transfected, negative control and blank control cells [（12.02 ± 0.70）, （12.71 ± 0.63） and （12.89 ± 0.71） mm, respectively; P 〉 0.05]. Conclusion： BRMS1 suppresses metastasis of GC cells, but does not inhibit growth of gastric tumors.展开更多
世界卫生组织(World Health Organization,WHO)国际癌症研究机构最新研究结果显示,乳腺癌是女性发病率最高的恶性肿瘤,严重危害女性健康,手术是治疗乳腺癌的主要方式之一。近年来,腔镜/机器人乳腺手术技术越来越受到广大医师和患者的青...世界卫生组织(World Health Organization,WHO)国际癌症研究机构最新研究结果显示,乳腺癌是女性发病率最高的恶性肿瘤,严重危害女性健康,手术是治疗乳腺癌的主要方式之一。近年来,腔镜/机器人乳腺手术技术越来越受到广大医师和患者的青睐,但手术方法多样、效果不一,为规范腔镜/机器人乳腺手术技术和方法,促进腔镜/机器人乳腺手术的推广和应用,更好地服务于广大乳腺疾病患者,中国乳腺腔镜/机器人手术临床研究协作组制定该指南,详细阐述腔镜/机器人乳腺手术相关的技术和设备、手术方法和技巧、围手术期管理及并发症处理、远期随访与结局等,旨在为乳腺疾病防控和诊治领域医务工作者提供指导和依据。展开更多
基金Supported by a grant from the Jiangxi Provincial Natural Science Foundation of China (No. 0640063)
文摘Objective： The aim of the study was to investigate inhibitory effects of breast-cancer metastasis suppressor 1 protein （BRMS1） on primary tumor growth and metastasis of human gastric cancer （GC） cells in nude mice. Methods： We compared the expression of BRMS1 in the primary gastric tumor and metastatic gastric tumor by immunohistochemistry. Expression of BRMS1 also was detected in the GC cells by RT-PCR and Western blot. Three groups of cultured human GC cell line SGC-7901, were maintained： transfected cells with pcDNA3.1（-）B/myc-BRMS1; negative control cells with pcD- NA3.1/myc-his（-）B; and blank control ceils without any transfection. Histologically intact samples of the cells, maintained by passage in the subcutis of nude mice, were transplanted orthotopically into stomach walls of nude mice to establish a nude mouse model of human gastric carcinoma. Their primary tumor growth and metastasis were then observed. Results： The expression of BRMS1 was markedly stronger in the primary gastric tumor compared with metastatic gastric tumor. We also detected BRMS1 gene and protein in the gastric cancer cell tines. Numbers of metastasis tumors significantly differed among mice infected with transfected cells, with negative controls and with blank controls （4.38 ± 0.60, 7.75 ± 0.59, and 7.63± 0.65, respectively; P 〈 0.05）. However, there were no significant differences in the size of orthotopic tumors among mice infected with transfected, negative control and blank control cells [（12.02 ± 0.70）, （12.71 ± 0.63） and （12.89 ± 0.71） mm, respectively; P 〉 0.05]. Conclusion： BRMS1 suppresses metastasis of GC cells, but does not inhibit growth of gastric tumors.
文摘世界卫生组织(World Health Organization,WHO)国际癌症研究机构最新研究结果显示,乳腺癌是女性发病率最高的恶性肿瘤,严重危害女性健康,手术是治疗乳腺癌的主要方式之一。近年来,腔镜/机器人乳腺手术技术越来越受到广大医师和患者的青睐,但手术方法多样、效果不一,为规范腔镜/机器人乳腺手术技术和方法,促进腔镜/机器人乳腺手术的推广和应用,更好地服务于广大乳腺疾病患者,中国乳腺腔镜/机器人手术临床研究协作组制定该指南,详细阐述腔镜/机器人乳腺手术相关的技术和设备、手术方法和技巧、围手术期管理及并发症处理、远期随访与结局等,旨在为乳腺疾病防控和诊治领域医务工作者提供指导和依据。
