B2-ordered Fe3Al single crystals with various orientations were deformed in tension at room temperature in vacuum. The shape of shear stress-strain curves and work hardening rates were found to be strongly dependent o...B2-ordered Fe3Al single crystals with various orientations were deformed in tension at room temperature in vacuum. The shape of shear stress-strain curves and work hardening rates were found to be strongly dependent on the orientation. The formation of the five different work hardening stages were considered to be related to the number of operative slip systems, the effect of secondary slip systems and the dissociation of the twofold superdislocation. Stage I is an easy glide stage corresponding to single slip. Stage II, with high hardening rate, often corresponds to the existence of conjugate slip systems. Stage III, with relatively low hardening rate, corresponds to the weaker hardening of secondary slip systems. Stage IV, with the highest hardening rate, is not only related to multiple slip but also the dissociation of twofold superdislocations and the moving of superpartials with an antiphase boundary (APB) trap. Stages V, with a negative hardening rate, may be caused by the cross slip of single dissociated superdislocation. The number of stages and the work hardening rate of the same stage were also found to change significantly, when the tensile orientation lies in different orientation regions.展开更多
Strokes include both ischemic stroke,which is mediated by a blockade or reduction in the blood supply to the brain,and hemorrhagic stroke,which comprises intracerebral hemorrhage and subarachnoid hemorrhage and is cha...Strokes include both ischemic stroke,which is mediated by a blockade or reduction in the blood supply to the brain,and hemorrhagic stroke,which comprises intracerebral hemorrhage and subarachnoid hemorrhage and is characterized by bleeding within the brain.Stroke is a lifethreatening cerebrovascular condition characterized by intricate pathophysiological mechanisms,including oxidative stress,inflammation,mitochondrial dysfunction,and neuronal injury.Critical transcription factors,such as nuclear factor erythroid 2-related factor 2 and nuclear factor kappa B,play central roles in the progression of stroke.Nuclear factor erythroid 2-related factor 2 is sensitive to changes in the cellular redox status and is crucial in protecting cells against oxidative damage,inflammatory responses,and cytotoxic agents.It plays a significant role in post-stroke neuroprotection and repair by influencing mitochondrial function,endoplasmic reticulum stress,and lysosomal activity and regulating metabolic pathways and cytokine expression.Conversely,nuclear factor-kappa B is closely associated with mitochondrial dysfunction,the generation of reactive oxygen species,oxidative stress exacerbation,and inflammation.Nuclear factor-kappa B contributes to neuronal injury,apoptosis,and immune responses following stroke by modulating cell adhesion molecules and inflammatory mediators.The interplay between these pathways,potentially involving crosstalk among various organelles,significantly influences stroke pathophysiology.Advancements in single-cell sequencing and spatial transcriptomics have greatly improved our understanding of stroke pathogenesis and offer new opportunities for the development of targeted,individualized,cell typespecific treatments.In this review,we discuss the mechanisms underlying the involvement of nuclear factor erythroid 2-related factor 2 and nuclear factor-kappa B in both ischemic and hemorrhagic stroke,with an emphasis on their roles in oxidative stress,inflammation,and neuroprotection.展开更多
实验建立了高效液相色谱法检测丹田降脂丸中23-乙酰泽泻醇B等3种成分含量的测定方法。Capcell Pak UG C_(18)色谱柱分离;检测波长:葡糖苷、23-乙酰泽泻醇B和淫羊藿苷检测波长分别设定为319nm、208nm和271nm;柱温:28℃;进样体积:10μL;...实验建立了高效液相色谱法检测丹田降脂丸中23-乙酰泽泻醇B等3种成分含量的测定方法。Capcell Pak UG C_(18)色谱柱分离;检测波长:葡糖苷、23-乙酰泽泻醇B和淫羊藿苷检测波长分别设定为319nm、208nm和271nm;柱温:28℃;进样体积:10μL;以乙腈为流动相A,1.0%磷酸溶液为流动相B,梯度洗脱,洗脱方法:0~5min 13%A,5~15min 13%~45%A,15~24min 45%~86%A,24~30min 86%A,30~31min 13%A,30~35min 13%A;外标法定量检测。结果表明,葡糖苷、23-乙酰泽泻醇B和淫羊藿苷在0.1896~18.96、0.0983~9.83和0.0981~9.81μg/mL范围内线性良好;平均回收率分别为99.83%、99.46%和99.14%;精密度和重复性RSD(n=6)均在5.0%以内;供试品溶液在24h内稳定。该方法检测时间短、操作简单,可以用于丹田降脂丸的质量控制。展开更多
基金This work was supported by the China Research and Development Fund (No. 59681005)
文摘B2-ordered Fe3Al single crystals with various orientations were deformed in tension at room temperature in vacuum. The shape of shear stress-strain curves and work hardening rates were found to be strongly dependent on the orientation. The formation of the five different work hardening stages were considered to be related to the number of operative slip systems, the effect of secondary slip systems and the dissociation of the twofold superdislocation. Stage I is an easy glide stage corresponding to single slip. Stage II, with high hardening rate, often corresponds to the existence of conjugate slip systems. Stage III, with relatively low hardening rate, corresponds to the weaker hardening of secondary slip systems. Stage IV, with the highest hardening rate, is not only related to multiple slip but also the dissociation of twofold superdislocations and the moving of superpartials with an antiphase boundary (APB) trap. Stages V, with a negative hardening rate, may be caused by the cross slip of single dissociated superdislocation. The number of stages and the work hardening rate of the same stage were also found to change significantly, when the tensile orientation lies in different orientation regions.
基金supported by grants from the Zhejiang Provincial TCM Science and Technology Plan Project,No.2023ZL156(to YH)Ningbo Top Medical and Health Research Program,No.2022020304(to XG)+1 种基金the Natural Science Foundation of Ningbo,No.2023J019(to YH)Key Laboratory of Precision Medicine for Atherosclerotic Diseases of Zhejiang Province,No.2022E10026(to YH)。
文摘Strokes include both ischemic stroke,which is mediated by a blockade or reduction in the blood supply to the brain,and hemorrhagic stroke,which comprises intracerebral hemorrhage and subarachnoid hemorrhage and is characterized by bleeding within the brain.Stroke is a lifethreatening cerebrovascular condition characterized by intricate pathophysiological mechanisms,including oxidative stress,inflammation,mitochondrial dysfunction,and neuronal injury.Critical transcription factors,such as nuclear factor erythroid 2-related factor 2 and nuclear factor kappa B,play central roles in the progression of stroke.Nuclear factor erythroid 2-related factor 2 is sensitive to changes in the cellular redox status and is crucial in protecting cells against oxidative damage,inflammatory responses,and cytotoxic agents.It plays a significant role in post-stroke neuroprotection and repair by influencing mitochondrial function,endoplasmic reticulum stress,and lysosomal activity and regulating metabolic pathways and cytokine expression.Conversely,nuclear factor-kappa B is closely associated with mitochondrial dysfunction,the generation of reactive oxygen species,oxidative stress exacerbation,and inflammation.Nuclear factor-kappa B contributes to neuronal injury,apoptosis,and immune responses following stroke by modulating cell adhesion molecules and inflammatory mediators.The interplay between these pathways,potentially involving crosstalk among various organelles,significantly influences stroke pathophysiology.Advancements in single-cell sequencing and spatial transcriptomics have greatly improved our understanding of stroke pathogenesis and offer new opportunities for the development of targeted,individualized,cell typespecific treatments.In this review,we discuss the mechanisms underlying the involvement of nuclear factor erythroid 2-related factor 2 and nuclear factor-kappa B in both ischemic and hemorrhagic stroke,with an emphasis on their roles in oxidative stress,inflammation,and neuroprotection.
文摘实验建立了高效液相色谱法检测丹田降脂丸中23-乙酰泽泻醇B等3种成分含量的测定方法。Capcell Pak UG C_(18)色谱柱分离;检测波长:葡糖苷、23-乙酰泽泻醇B和淫羊藿苷检测波长分别设定为319nm、208nm和271nm;柱温:28℃;进样体积:10μL;以乙腈为流动相A,1.0%磷酸溶液为流动相B,梯度洗脱,洗脱方法:0~5min 13%A,5~15min 13%~45%A,15~24min 45%~86%A,24~30min 86%A,30~31min 13%A,30~35min 13%A;外标法定量检测。结果表明,葡糖苷、23-乙酰泽泻醇B和淫羊藿苷在0.1896~18.96、0.0983~9.83和0.0981~9.81μg/mL范围内线性良好;平均回收率分别为99.83%、99.46%和99.14%;精密度和重复性RSD(n=6)均在5.0%以内;供试品溶液在24h内稳定。该方法检测时间短、操作简单,可以用于丹田降脂丸的质量控制。
