In the field of nerve repair,one major challenge is the formation of neuroma.However,reports on both the promotion of nerve regeneration and prevention of traumatic neuroma in the clinical settings are rare in the fie...In the field of nerve repair,one major challenge is the formation of neuroma.However,reports on both the promotion of nerve regeneration and prevention of traumatic neuroma in the clinical settings are rare in the field of nerve repair.One of the reasons could be the insufficiency in the follow-up system.We have conducted 33 cases of nerve repair using PRGD/PDLLA/b-TCP conduit without any sign of adverse reaction,especially no neuroma formation.Among them,we have selected two cases as representatives to report in this article.The first case was a patient with an upper limb nerve wound was bridged by PRGD/PDLLA/b-TCP conduit and a plate fixation was given.After nearly 3-years’follow-up,the examination results demonstrated that nerve regeneration effect was very good.When the reoperation was performed to remove the steel plate we observed a uniform structure of the regenerated nerve without the formation of neuroma,and to our delight,the implanted conduit was completely degraded 23 months after the implantation.The second case had an obsolete nerve injury with neuroma formation.After removal of the neuroma,the nerve was bridged by PRGD/PDLLA/b-TCP conduit.Follow-up examinations showed that the structure and functional recovery were improved gradually in the 10-month follow-up;no end-enlargement and any other abnormal reaction associated with the characteristic of neuroma were found.Based on our 33-case studies,we have concluded that PRGD/PDLLA/b-TCP nerve conduit could both promote nerve regeneration and prevent neuroma formation;therefore,it is a good alternative for peripheral nerve repair.展开更多
The combination between biphasic calcium phosphate(BCP)and the osteomimetic porous microstructure obtained via freeze casting is hoped to achieve excellent bone regeneration,while the effects of HA and b-TCP ratio cha...The combination between biphasic calcium phosphate(BCP)and the osteomimetic porous microstructure obtained via freeze casting is hoped to achieve excellent bone regeneration,while the effects of HA and b-TCP ratio changes on the degradation and biological performance of the BCP scaffolds with this unique microstructure need to be determined.Here,we prepared the osteomimetic BCP scaffolds with different HA/b-TCP ratios(HA30/b-TCP70,HA50/b-TCP50,HA70/b-TCP30)and the effects of different HA/b-TCPHA/b-TCP ratios on the degradation and biological performance were studied in vitro and vivo.These BCP scaffolds with different HA/b-TCP ratios exhibited similar microstructure,mechanical performance,and protein absorption capability,while HA70/b-TCP30 BCP scaffolds showed an advisable degradation rate.Study in vitro confirmed the bio-compatibility and promotion on the proliferation,differentiation of MG63 cells in the porous osteomimetic BCP scaffolds with a HA/b-TCP ratio at 30:70.Implantation experiments also showed that the porous osteomimetic BCP scaffolds with a HA/b-TCP ratio at 30:70 had excellent bone regeneration capacity and proper degradation rate compatible with bone growth.These results reveal that the porous osteomimetic BCP scaffold with a HA/b-TCP ratio at 30:70 is a potential candidate of biodegradable bone substitutes used for bone repair.展开更多
基金This work was supported by the National Basic Research Program of China(973 Program,No.2011CB606205)National Natural Science Foundation of China(No.51403168,81190133 and 51172172)+1 种基金Natural Science Foundation of Hubei Province(No.2013CFB354)Key grant Project of Chinese Ministry of Education(No.313041).
文摘In the field of nerve repair,one major challenge is the formation of neuroma.However,reports on both the promotion of nerve regeneration and prevention of traumatic neuroma in the clinical settings are rare in the field of nerve repair.One of the reasons could be the insufficiency in the follow-up system.We have conducted 33 cases of nerve repair using PRGD/PDLLA/b-TCP conduit without any sign of adverse reaction,especially no neuroma formation.Among them,we have selected two cases as representatives to report in this article.The first case was a patient with an upper limb nerve wound was bridged by PRGD/PDLLA/b-TCP conduit and a plate fixation was given.After nearly 3-years’follow-up,the examination results demonstrated that nerve regeneration effect was very good.When the reoperation was performed to remove the steel plate we observed a uniform structure of the regenerated nerve without the formation of neuroma,and to our delight,the implanted conduit was completely degraded 23 months after the implantation.The second case had an obsolete nerve injury with neuroma formation.After removal of the neuroma,the nerve was bridged by PRGD/PDLLA/b-TCP conduit.Follow-up examinations showed that the structure and functional recovery were improved gradually in the 10-month follow-up;no end-enlargement and any other abnormal reaction associated with the characteristic of neuroma were found.Based on our 33-case studies,we have concluded that PRGD/PDLLA/b-TCP nerve conduit could both promote nerve regeneration and prevent neuroma formation;therefore,it is a good alternative for peripheral nerve repair.
基金This study was financially supported by the National Natural Science Foundation of China(No.81571021).
文摘The combination between biphasic calcium phosphate(BCP)and the osteomimetic porous microstructure obtained via freeze casting is hoped to achieve excellent bone regeneration,while the effects of HA and b-TCP ratio changes on the degradation and biological performance of the BCP scaffolds with this unique microstructure need to be determined.Here,we prepared the osteomimetic BCP scaffolds with different HA/b-TCP ratios(HA30/b-TCP70,HA50/b-TCP50,HA70/b-TCP30)and the effects of different HA/b-TCPHA/b-TCP ratios on the degradation and biological performance were studied in vitro and vivo.These BCP scaffolds with different HA/b-TCP ratios exhibited similar microstructure,mechanical performance,and protein absorption capability,while HA70/b-TCP30 BCP scaffolds showed an advisable degradation rate.Study in vitro confirmed the bio-compatibility and promotion on the proliferation,differentiation of MG63 cells in the porous osteomimetic BCP scaffolds with a HA/b-TCP ratio at 30:70.Implantation experiments also showed that the porous osteomimetic BCP scaffolds with a HA/b-TCP ratio at 30:70 had excellent bone regeneration capacity and proper degradation rate compatible with bone growth.These results reveal that the porous osteomimetic BCP scaffold with a HA/b-TCP ratio at 30:70 is a potential candidate of biodegradable bone substitutes used for bone repair.
