Hepatitis C virus(HCV)and hepatitis B virus(HBV)infections are increasingly recognized as significant etiological factors in the pathogenesis of B-cell non-Hodgkin’s lymphomas(B-NHLs).Epidemiological and molecular st...Hepatitis C virus(HCV)and hepatitis B virus(HBV)infections are increasingly recognized as significant etiological factors in the pathogenesis of B-cell non-Hodgkin’s lymphomas(B-NHLs).Epidemiological and molecular studies have demonstrated a consistent association between chronic viral infection and B-NHLs.Multiple pathogenic mechanisms have been implicated in lymphomagenesis,both direct and indirect,including chronic antigenic stimulation,direct infection of B cells,and viral protein-mediated oncogenic signaling,It is likely that a combination of several pathogenic conditions is required to eventually lead to the development of lymphoma.The prevalence of B-cell lymphomas among individuals with chronic HCV or HBV infection presents a complex pathogenetic scenario,given the tumor heterogeneity and variable clinical behavior,and poses therapeutic challenges,due to the partial efficacy of current treatment options.The advent of direct-acting antivirals(DAAs)for HCV and high-genetic barrier nucleos(t)ide analogues(NAs)for HBV has improved patient outcomes.In indolent HCV-associated B-NHLs,antiviral therapy with DAAs alone often achieves sustained virologic response and may lead to lymphoma regression.Conversely,aggressive subtypes like diffuse large B-cell lymphomas require combination treatment with immunochemotherapy.In the setting of HBV-associated lymphomas,antiviral prophylaxis with potent NAs(e.g.,entecavir or tenofovir)is essential to prevent HBV reactivation during rituximab-containing chemotherapy regimen.The integration of antiviral and anticancer therapies has been shown to enhance survival outcomes while mitigating hepatic toxicity.A comprehensive understanding of the biological interplay between chronic viral infection and B-cell transformation is critical for optimizing diagnostic and therapeutic strategies.Aim of this viewpoint is to provide evidence that early viral detection and prompt management remain the most effective strategies to improve survival rates and to reduce treatment-related morbidity in these patients.展开更多
The development of digital construction management is an important initiative to promote the digital transformation of the construction industry. But the attention to the regional differences in the development level ...The development of digital construction management is an important initiative to promote the digital transformation of the construction industry. But the attention to the regional differences in the development level of digital construction management in China from the industrial level is still relatively scarce. In this paper, the combination assignment method, Dagum’s Gini coefficient and Kernel density estimation method, are used to explore the regional differences and their dynamic evolution trends of China’s digital construction management development level. The study finds that the overall development level in China’s construction industry is on the rise, but it is still at a relatively low level. The overall Gini coefficient has increased, which is mainly due to uneven development between regions. There are large development differences between the eastern region and the other three regions. The interregional Gini coefficients for the Central-Northeastern and Central-Western regions are all growing at a higher rate.展开更多
The idea of a network society was introduced by Western sociologists at the end of the 20th century after in-depth research was conducted from perspectives such as informationalism.Influenced by these developments,the...The idea of a network society was introduced by Western sociologists at the end of the 20th century after in-depth research was conducted from perspectives such as informationalism.Influenced by these developments,the concept of constructing a network society also emerged in China.Over the past 30 years,China has made significant progress and achievements in constructing a network society,both in terms of its fundamental construction and social development.It is important that these advancements be summarized and reviewed.China’s network society construction can be divided into two relatively independent yet interconnected components,based on their focal points:its foundational infrastructure and its social development.These two components of China’s network society are managed by different departments.China has integrated the fundamental construction of its network society with the social development of its network society,thereby achieving unified planning,collaborative advancement,and coordinated development.This approach aims to harmonize two aspects:building China’s cyberspace strength and contributing to Chinese informatization,thereby advancing Chinese modernization.展开更多
As an emerging media format with immense growth potential,micro-dramas(MDs)carry the critical mission of inheriting and innovating China’s fine traditional culture in the new era.From the three dimensions of time,spa...As an emerging media format with immense growth potential,micro-dramas(MDs)carry the critical mission of inheriting and innovating China’s fine traditional culture in the new era.From the three dimensions of time,space,and landscape in media geography,the pathways for cultural inheritance and innovation through MDs can be summarized as follows:Temporally,cultural MDs construct cultural narratives from the“past”to the“future;”spatially,they build immersive cultural spaces blending reality and imagination,enhancing viewers’audiovisual experiences;through the interplay of time and space,they create diversified cultural landscapes on new media platforms,revitalize enduring cultural memories and foster a sense of community for the Chinese nation through media innovation.展开更多
Urban construction land suitability evaluation （UCLSE） is a complex system engineering and the basis for rational use of the limited urban land resources in China. It has an important practical value on urban constr...Urban construction land suitability evaluation （UCLSE） is a complex system engineering and the basis for rational use of the limited urban land resources in China. It has an important practical value on urban construction land use planning and management from the angle of methodology. As a widely used technique, traditional multi-criteria evaluation based on GIS （MCE-GIS）, is not suitable for UCLSE. This study develops an improved MCE-GIS method which could be more suitable for UCLSE based on urban complex ecological system theory and the summary of the shortcomings of traditional MCE-GIS. The improvements include three aspects： a composite evaluation index system rather than natural indexes alone, an index weight calculated by using fuzzy Analytic Hierarchy Process （AHP） method rather than the common AHP method, and the integrated overlay rule, which includes selecting the minimum value, weighted linear combination （WLC） and simple summation. The main advantage of this improved technique is that it can make UCLSE more comprehensive, more operational and more reasonable. It can provide a scientific basis for decision making in the planning and management of urban construction land use. The improved MCE-GIS system has been adopted in the New Hefei City, Anhui Province, China. Based on the results of UCLSE in New Hefei, three functional areas including construction-appropriate areas, construction-restricted areas and construction-forbidden areas could be worked out, in which 36.90% of the total study area could be developed as urban construction land and the remaining 63.10% should be protected as reserves land or as ecological land. Furthermore, the results can orovide scientific decision suooort for spatial planning and eco-environment nrotection in New Hefei.展开更多
In this work,history of education is expressed and evaluated generally/specifically by considering the related concepts.Definition and types of education explained with specific approaches in the literature.Early chil...In this work,history of education is expressed and evaluated generally/specifically by considering the related concepts.Definition and types of education explained with specific approaches in the literature.Early childhood development briefly defined and the correlation with education systems outlined.Five education evolution phases expressed with its basic concepts.The meaning and importance of philosophy of education are then defined according to the general approaches.Some of the important philosophers of education and their philosophical/scientific interests are compared and expressed with a table.New perspective of the philosophy generally defined by considering new era philosophy,eight basic philosophies,and hybrid philosophies.R-Philosophy,R-Science,R-Information,R-Education,and R-System new disciplines are shortly expressed.New perspective for the philosophy of education defined as sub-branch of philosophy of information and explained regarding the basic information on the related theories.17 education components are defined for continuable/sustainable education,and efficiency,effective use,and improvement/development of each of the components expressed specifically.Performance of the education components for a country-X is given with a figure as an example.Relation between education,values,and basic senses is interpreted.Evaluation of the education due to 17 specific perspectives is generally mentioned.Then,evaluation of the education from philosophical,academic,and administration perspectives is specifically explained.Continuable/sustainable education administration system for a world country is defined with figures.Education system administration is defined with bloc diagrams accordingly.Ideal political/nonpolitical education construction for a progressive world country is outlined with 5x5 table,and related explanations done.Then importance of education due to 39 subjects of services is expressed with a table.Categories of education programs outlined.Digital education&digital tools and engineering education and related five industry evolution phases are defined as special education programs.New proposal is given for Education 5.0 and Engineering Education 5.0.展开更多
Objective: To construct a cDNA library from human liver tissue with chronic hepatitis B and check its quality for investigating the expression level of liver tissue infected by hepatitis B virus. This will then be use...Objective: To construct a cDNA library from human liver tissue with chronic hepatitis B and check its quality for investigating the expression level of liver tissue infected by hepatitis B virus. This will then be used to find the relevant genes and interesting proteins associated with the development of hepatitis B. Methods: The total RNA from liver tissue with chronic hepa- titis B was extracted and the mRNA was purified using TRIZOL method. Switching mechanism at 5′ end of the RNA transcript (SMART) technique and CDS III/3′ primer were used for first-strand cDNA synthesis. Long distance polymerase chain reaction (LD PCR) was then used to synthesize the double-strand cDNA that was then digested by Sfi I and fractionated by CHROMA SPIN-400 column. The longer than 0.4 kb cDNAs were collected and ligated to λTriplEx2 vector. Then λ phage packaging reaction and library amplification were performed. The qualities of both unamplified and amplified cDNA libraries were strictly checked by conventional titer determination. Fourteen plaques were randomly picked and tested using PCR with universal primers derived from the sequence flanking the vector. Results: The titers of unamplifed and amplified libraries were 1.94×106 pfu/ml and 1.49×109 pfu/ml respectively. The percentages of recombinants from both libraries were 98.15% in unamplified library and 98.76% in amplified library. The lengths of the inserts were 1.23 kb in average, 1?2 kb in 64.29%, and 0.5?1.0 kb in 35.71%. Conclusion: A high quality cDNA library from human liver tissue with chronic hepatitis B was successfully constructed.展开更多
AIM: To characterize the biochemical and immunological properties of an experimental ISCOMS vaccine prepared from a novel therapeutic polypeptide based on T cell epitopes of HBsAg, and a heptatis B-ISCOMS was prepared...AIM: To characterize the biochemical and immunological properties of an experimental ISCOMS vaccine prepared from a novel therapeutic polypeptide based on T cell epitopes of HBsAg, and a heptatis B-ISCOMS was prepared and investigated. METHODS: An immunostimulating complexes(ISCOMS)-based vaccine containing a novel therapeutic hepatitis B polypeptide was prepared by dialysis method, and its formation was visualized by electron microscopy and biochemically verified by SDS-polyacrylamide gel electrophoresis. Amount of the peptide within ISCOMS was determined by Bradford assay, and specific CTL response was detected by ELISPOT assay. RESULTS: Typical cage-like structures of submicroparticle with a diameter of about 40nm were observed by electron microscopy. Results from Bradford assay showed that the level of peptide incorporation was about 0.33g.L(-1). At the paralleled position close to the sixth band of the molecular weight marker(3480kDa) a clear band was shown in SDS-PAGE analysis, indicating successful incorporation of polypeptide into ISCOMS. It is suggested that ISCOMS delivery system could efficiently improve the immunogenicity of polypeptide and elicit specific immune responses in vivo by the results of ELISPOT assay, which showed that IFN-gamma producing cells(specific CTL responses) were increased(spots of ISCOMS-treated group: 47+/-5, n =3; control group: 5+/-2, n =3). CONCLUSION: ISCOMS-based hepatitis B polypeptide vaccine is successfully constructed and it induces a higher CTL response compared with short polypeptides vaccine in vivo.展开更多
The existing early-warning system in metro construction are generally based on traditional single-sensor data and simple analytic model, which makes it difficult to deal with the complex and comprehensive environment ...The existing early-warning system in metro construction are generally based on traditional single-sensor data and simple analytic model, which makes it difficult to deal with the complex and comprehensive environment in metro construction. In this paper, the framework of early-warning threshold for metro construction collapse risk based on D-S evidence theory and rough set is built. By combining the primary data fusion collected based on rough set with the secondary data fusion which is based on D-S evidence theory, the integration of multiple information in metro construction is realized and the risk assessment methods are optimized. A case trial based on Hangzhou metro construction collapse accident is also carried out to exemplify the framework. The empirical analysis guarantees the completeness and independence of the prediction information, and realizes the dynamic prediction of the variation trend of metro construction collapse risk.展开更多
Screening humanized antibodies from a human Fab phage display library is an effective and quick method to obtain beta-amyloid oligomers. Thus, the present study prepared amyloid-beta 42 oli- gomers and constructed a h...Screening humanized antibodies from a human Fab phage display library is an effective and quick method to obtain beta-amyloid oligomers. Thus, the present study prepared amyloid-beta 42 oli- gomers and constructed a have human Fab phage display library based on blood samples from six healthy people. After three rounds of biopanning in vitro, a human single-domain antibody that spe- cifically recognized amyloid-beta 42 oligomers was identified. Western blot and enzyme-linked im- munosorbent assay demonstrated this antibody bound specifically to human amyloid-beta 42 tetramer and nonamer, but not the monomer or high molecular weight oligomers. This study suc- cessfully constructed a human phage display library and screened a single-domain antibody that specifically recognized amyloid-beta 42 oligomers.展开更多
The relationship between children's activities and public spaces was analyzed and summarized from three perspectives, namely, children's demands on outdoor activity spaces, factors infl uencing children's ...The relationship between children's activities and public spaces was analyzed and summarized from three perspectives, namely, children's demands on outdoor activity spaces, factors infl uencing children's behavior requirements, and planning strategies. The paper provided theoretical support for exploring the relationship between children's activities and public spaces, and proposed planning strategies for improving use effi ciency of urban outdoor public spaces and facilities, and creating public spaces capable of meeting children's psychological needs.展开更多
On the eve of the grand celebrations marking the 45th anniversary for the founding of new China, the People’s Construction Bank of China, one of our country’s four pillars of the banking industry, welcomed its 40th ...On the eve of the grand celebrations marking the 45th anniversary for the founding of new China, the People’s Construction Bank of China, one of our country’s four pillars of the banking industry, welcomed its 40th birthday. The Government Administration Council decided on September 9, 1954, to establish the People’s Construction Bank of China to handle mainly appropriations for capital construction and floating capital loans for construction enterprises toexercise financial supervision of the rational use展开更多
基金supported by the National Italian Research Council(CNR)“Progetto DSB.AD007.305.001”to Monica Rinaldi。
文摘Hepatitis C virus(HCV)and hepatitis B virus(HBV)infections are increasingly recognized as significant etiological factors in the pathogenesis of B-cell non-Hodgkin’s lymphomas(B-NHLs).Epidemiological and molecular studies have demonstrated a consistent association between chronic viral infection and B-NHLs.Multiple pathogenic mechanisms have been implicated in lymphomagenesis,both direct and indirect,including chronic antigenic stimulation,direct infection of B cells,and viral protein-mediated oncogenic signaling,It is likely that a combination of several pathogenic conditions is required to eventually lead to the development of lymphoma.The prevalence of B-cell lymphomas among individuals with chronic HCV or HBV infection presents a complex pathogenetic scenario,given the tumor heterogeneity and variable clinical behavior,and poses therapeutic challenges,due to the partial efficacy of current treatment options.The advent of direct-acting antivirals(DAAs)for HCV and high-genetic barrier nucleos(t)ide analogues(NAs)for HBV has improved patient outcomes.In indolent HCV-associated B-NHLs,antiviral therapy with DAAs alone often achieves sustained virologic response and may lead to lymphoma regression.Conversely,aggressive subtypes like diffuse large B-cell lymphomas require combination treatment with immunochemotherapy.In the setting of HBV-associated lymphomas,antiviral prophylaxis with potent NAs(e.g.,entecavir or tenofovir)is essential to prevent HBV reactivation during rituximab-containing chemotherapy regimen.The integration of antiviral and anticancer therapies has been shown to enhance survival outcomes while mitigating hepatic toxicity.A comprehensive understanding of the biological interplay between chronic viral infection and B-cell transformation is critical for optimizing diagnostic and therapeutic strategies.Aim of this viewpoint is to provide evidence that early viral detection and prompt management remain the most effective strategies to improve survival rates and to reduce treatment-related morbidity in these patients.
文摘The development of digital construction management is an important initiative to promote the digital transformation of the construction industry. But the attention to the regional differences in the development level of digital construction management in China from the industrial level is still relatively scarce. In this paper, the combination assignment method, Dagum’s Gini coefficient and Kernel density estimation method, are used to explore the regional differences and their dynamic evolution trends of China’s digital construction management development level. The study finds that the overall development level in China’s construction industry is on the rise, but it is still at a relatively low level. The overall Gini coefficient has increased, which is mainly due to uneven development between regions. There are large development differences between the eastern region and the other three regions. The interregional Gini coefficients for the Central-Northeastern and Central-Western regions are all growing at a higher rate.
基金“Research on Social Change and Network Society Planning in the Internet of Everything Era”(ID:21BSH005),a project under the National Social Science Fund of China
文摘The idea of a network society was introduced by Western sociologists at the end of the 20th century after in-depth research was conducted from perspectives such as informationalism.Influenced by these developments,the concept of constructing a network society also emerged in China.Over the past 30 years,China has made significant progress and achievements in constructing a network society,both in terms of its fundamental construction and social development.It is important that these advancements be summarized and reviewed.China’s network society construction can be divided into two relatively independent yet interconnected components,based on their focal points:its foundational infrastructure and its social development.These two components of China’s network society are managed by different departments.China has integrated the fundamental construction of its network society with the social development of its network society,thereby achieving unified planning,collaborative advancement,and coordinated development.This approach aims to harmonize two aspects:building China’s cyberspace strength and contributing to Chinese informatization,thereby advancing Chinese modernization.
基金supported by“Reliable,Admirable,Respectable:The Inheritance and Innovation of Li Bai’s Image in ACG Media”(Project No.LB24-A05)funded by the Research Center of Libai,a Key Research Base for Philosophy and Social Sciences in Sichuan Province,“Mechanisms for Enhancing the Modern Dissemination of Three Kingdoms Figures Through Short-Form Dramas”(Project No.24ZGL05).
文摘As an emerging media format with immense growth potential,micro-dramas(MDs)carry the critical mission of inheriting and innovating China’s fine traditional culture in the new era.From the three dimensions of time,space,and landscape in media geography,the pathways for cultural inheritance and innovation through MDs can be summarized as follows:Temporally,cultural MDs construct cultural narratives from the“past”to the“future;”spatially,they build immersive cultural spaces blending reality and imagination,enhancing viewers’audiovisual experiences;through the interplay of time and space,they create diversified cultural landscapes on new media platforms,revitalize enduring cultural memories and foster a sense of community for the Chinese nation through media innovation.
基金Under the auspices of National Natural Science Foundation of China(No.41201168)Fundamental Research Funds for the Central Universities(No.2013HGXJ0207)
文摘Urban construction land suitability evaluation （UCLSE） is a complex system engineering and the basis for rational use of the limited urban land resources in China. It has an important practical value on urban construction land use planning and management from the angle of methodology. As a widely used technique, traditional multi-criteria evaluation based on GIS （MCE-GIS）, is not suitable for UCLSE. This study develops an improved MCE-GIS method which could be more suitable for UCLSE based on urban complex ecological system theory and the summary of the shortcomings of traditional MCE-GIS. The improvements include three aspects： a composite evaluation index system rather than natural indexes alone, an index weight calculated by using fuzzy Analytic Hierarchy Process （AHP） method rather than the common AHP method, and the integrated overlay rule, which includes selecting the minimum value, weighted linear combination （WLC） and simple summation. The main advantage of this improved technique is that it can make UCLSE more comprehensive, more operational and more reasonable. It can provide a scientific basis for decision making in the planning and management of urban construction land use. The improved MCE-GIS system has been adopted in the New Hefei City, Anhui Province, China. Based on the results of UCLSE in New Hefei, three functional areas including construction-appropriate areas, construction-restricted areas and construction-forbidden areas could be worked out, in which 36.90% of the total study area could be developed as urban construction land and the remaining 63.10% should be protected as reserves land or as ecological land. Furthermore, the results can orovide scientific decision suooort for spatial planning and eco-environment nrotection in New Hefei.
文摘In this work,history of education is expressed and evaluated generally/specifically by considering the related concepts.Definition and types of education explained with specific approaches in the literature.Early childhood development briefly defined and the correlation with education systems outlined.Five education evolution phases expressed with its basic concepts.The meaning and importance of philosophy of education are then defined according to the general approaches.Some of the important philosophers of education and their philosophical/scientific interests are compared and expressed with a table.New perspective of the philosophy generally defined by considering new era philosophy,eight basic philosophies,and hybrid philosophies.R-Philosophy,R-Science,R-Information,R-Education,and R-System new disciplines are shortly expressed.New perspective for the philosophy of education defined as sub-branch of philosophy of information and explained regarding the basic information on the related theories.17 education components are defined for continuable/sustainable education,and efficiency,effective use,and improvement/development of each of the components expressed specifically.Performance of the education components for a country-X is given with a figure as an example.Relation between education,values,and basic senses is interpreted.Evaluation of the education due to 17 specific perspectives is generally mentioned.Then,evaluation of the education from philosophical,academic,and administration perspectives is specifically explained.Continuable/sustainable education administration system for a world country is defined with figures.Education system administration is defined with bloc diagrams accordingly.Ideal political/nonpolitical education construction for a progressive world country is outlined with 5x5 table,and related explanations done.Then importance of education due to 39 subjects of services is expressed with a table.Categories of education programs outlined.Digital education&digital tools and engineering education and related five industry evolution phases are defined as special education programs.New proposal is given for Education 5.0 and Engineering Education 5.0.
基金Project (No. 30371270) supported by the National Natural Science Foundation of China
文摘Objective: To construct a cDNA library from human liver tissue with chronic hepatitis B and check its quality for investigating the expression level of liver tissue infected by hepatitis B virus. This will then be used to find the relevant genes and interesting proteins associated with the development of hepatitis B. Methods: The total RNA from liver tissue with chronic hepa- titis B was extracted and the mRNA was purified using TRIZOL method. Switching mechanism at 5′ end of the RNA transcript (SMART) technique and CDS III/3′ primer were used for first-strand cDNA synthesis. Long distance polymerase chain reaction (LD PCR) was then used to synthesize the double-strand cDNA that was then digested by Sfi I and fractionated by CHROMA SPIN-400 column. The longer than 0.4 kb cDNAs were collected and ligated to λTriplEx2 vector. Then λ phage packaging reaction and library amplification were performed. The qualities of both unamplified and amplified cDNA libraries were strictly checked by conventional titer determination. Fourteen plaques were randomly picked and tested using PCR with universal primers derived from the sequence flanking the vector. Results: The titers of unamplifed and amplified libraries were 1.94×106 pfu/ml and 1.49×109 pfu/ml respectively. The percentages of recombinants from both libraries were 98.15% in unamplified library and 98.76% in amplified library. The lengths of the inserts were 1.23 kb in average, 1?2 kb in 64.29%, and 0.5?1.0 kb in 35.71%. Conclusion: A high quality cDNA library from human liver tissue with chronic hepatitis B was successfully constructed.
基金the National Natural Science Foundation of China,No.39789010
文摘AIM: To characterize the biochemical and immunological properties of an experimental ISCOMS vaccine prepared from a novel therapeutic polypeptide based on T cell epitopes of HBsAg, and a heptatis B-ISCOMS was prepared and investigated. METHODS: An immunostimulating complexes(ISCOMS)-based vaccine containing a novel therapeutic hepatitis B polypeptide was prepared by dialysis method, and its formation was visualized by electron microscopy and biochemically verified by SDS-polyacrylamide gel electrophoresis. Amount of the peptide within ISCOMS was determined by Bradford assay, and specific CTL response was detected by ELISPOT assay. RESULTS: Typical cage-like structures of submicroparticle with a diameter of about 40nm were observed by electron microscopy. Results from Bradford assay showed that the level of peptide incorporation was about 0.33g.L(-1). At the paralleled position close to the sixth band of the molecular weight marker(3480kDa) a clear band was shown in SDS-PAGE analysis, indicating successful incorporation of polypeptide into ISCOMS. It is suggested that ISCOMS delivery system could efficiently improve the immunogenicity of polypeptide and elicit specific immune responses in vivo by the results of ELISPOT assay, which showed that IFN-gamma producing cells(specific CTL responses) were increased(spots of ISCOMS-treated group: 47+/-5, n =3; control group: 5+/-2, n =3). CONCLUSION: ISCOMS-based hepatitis B polypeptide vaccine is successfully constructed and it induces a higher CTL response compared with short polypeptides vaccine in vivo.
基金Supported by the National Natural Science Foundation of China(71603284)the Humanity and Social Science Research Foundation of Ministry of Education(16YJC630068)
文摘The existing early-warning system in metro construction are generally based on traditional single-sensor data and simple analytic model, which makes it difficult to deal with the complex and comprehensive environment in metro construction. In this paper, the framework of early-warning threshold for metro construction collapse risk based on D-S evidence theory and rough set is built. By combining the primary data fusion collected based on rough set with the secondary data fusion which is based on D-S evidence theory, the integration of multiple information in metro construction is realized and the risk assessment methods are optimized. A case trial based on Hangzhou metro construction collapse accident is also carried out to exemplify the framework. The empirical analysis guarantees the completeness and independence of the prediction information, and realizes the dynamic prediction of the variation trend of metro construction collapse risk.
基金supported by the National Natural Science Foundation of China,No.30600099(FD)
文摘Screening humanized antibodies from a human Fab phage display library is an effective and quick method to obtain beta-amyloid oligomers. Thus, the present study prepared amyloid-beta 42 oli- gomers and constructed a have human Fab phage display library based on blood samples from six healthy people. After three rounds of biopanning in vitro, a human single-domain antibody that spe- cifically recognized amyloid-beta 42 oligomers was identified. Western blot and enzyme-linked im- munosorbent assay demonstrated this antibody bound specifically to human amyloid-beta 42 tetramer and nonamer, but not the monomer or high molecular weight oligomers. This study suc- cessfully constructed a human phage display library and screened a single-domain antibody that specifically recognized amyloid-beta 42 oligomers.
基金Sponsored by Graduates’Innovation Foundation(YC2015-S133)
文摘The relationship between children's activities and public spaces was analyzed and summarized from three perspectives, namely, children's demands on outdoor activity spaces, factors infl uencing children's behavior requirements, and planning strategies. The paper provided theoretical support for exploring the relationship between children's activities and public spaces, and proposed planning strategies for improving use effi ciency of urban outdoor public spaces and facilities, and creating public spaces capable of meeting children's psychological needs.
文摘On the eve of the grand celebrations marking the 45th anniversary for the founding of new China, the People’s Construction Bank of China, one of our country’s four pillars of the banking industry, welcomed its 40th birthday. The Government Administration Council decided on September 9, 1954, to establish the People’s Construction Bank of China to handle mainly appropriations for capital construction and floating capital loans for construction enterprises toexercise financial supervision of the rational use
