The bacterium Avibacterium paragallinarum,previously known as Haemophilus paragallinarum,is responsible for caus-ing infectious coryza(IC)in chickens and other avian species.In this case report,an outbreak of Avibacte...The bacterium Avibacterium paragallinarum,previously known as Haemophilus paragallinarum,is responsible for caus-ing infectious coryza(IC)in chickens and other avian species.In this case report,an outbreak of Avibacterium paragal-linarum occurred in the Qinling area of China,resulting in clinical symptoms of facial swelling in several bird species,including Golden pheasant,Temminck's tragopan,and Peafowls,and three Golden pheasants died due to prolonged infection.Specific PCR results confrmed the presence of the pathogen in the infected birds.The report describes the clinical symptoms and pathological changes observed in the affected birds,as well as the isolation and identification of Avibacterium paragallinarum.Whole-genome sequencing and phylogenetic anal sis y were performed,and this is the frst report of inter-and intra-species transmission of infectious coryza among wild birds in China.展开更多
应用环介导等温扩增(LAMP)技术建立了快速特异的检测副鸡禽杆菌的方法。利用在线引物设计软件Primer Explorer Version 4.0,针对副鸡禽杆菌16SRNA基因序列设计两组LAMP引物,并对副鸡禽杆菌特异性DNA的扩增条件进行优化,评价所建立LAMP...应用环介导等温扩增(LAMP)技术建立了快速特异的检测副鸡禽杆菌的方法。利用在线引物设计软件Primer Explorer Version 4.0,针对副鸡禽杆菌16SRNA基因序列设计两组LAMP引物,并对副鸡禽杆菌特异性DNA的扩增条件进行优化,评价所建立LAMP的特异性和灵敏性。结果显示,优化后的反应条件为65℃恒温反应60min,内引物1.60pmol/μL、外引物0.20pmol/μL。该LAMP对13株副鸡禽杆菌均发生了扩增反应,而对大肠埃希菌、禽多杀性巴氏杆菌、空肠弯曲杆菌等14株其他病原微生物没有扩增反应;该LAMP最低检出量为0.17pg/反应,敏感性远高于常规PCR的56pg/反应。结果表明,所建立的副鸡禽杆菌LAMP具有快速、高效、方便操作等特点,适用于基层兽医部门进行副鸡禽杆菌的快速检测。展开更多
基金This study has been supported by Shaanxi Special Fund Project of Giant Panda International Cooperation(Panda Protection Letter[2019]No.93)the Project of Course Case Library Construction(Grant No.Z1050221011).
文摘The bacterium Avibacterium paragallinarum,previously known as Haemophilus paragallinarum,is responsible for caus-ing infectious coryza(IC)in chickens and other avian species.In this case report,an outbreak of Avibacterium paragal-linarum occurred in the Qinling area of China,resulting in clinical symptoms of facial swelling in several bird species,including Golden pheasant,Temminck's tragopan,and Peafowls,and three Golden pheasants died due to prolonged infection.Specific PCR results confrmed the presence of the pathogen in the infected birds.The report describes the clinical symptoms and pathological changes observed in the affected birds,as well as the isolation and identification of Avibacterium paragallinarum.Whole-genome sequencing and phylogenetic anal sis y were performed,and this is the frst report of inter-and intra-species transmission of infectious coryza among wild birds in China.
文摘应用环介导等温扩增(LAMP)技术建立了快速特异的检测副鸡禽杆菌的方法。利用在线引物设计软件Primer Explorer Version 4.0,针对副鸡禽杆菌16SRNA基因序列设计两组LAMP引物,并对副鸡禽杆菌特异性DNA的扩增条件进行优化,评价所建立LAMP的特异性和灵敏性。结果显示,优化后的反应条件为65℃恒温反应60min,内引物1.60pmol/μL、外引物0.20pmol/μL。该LAMP对13株副鸡禽杆菌均发生了扩增反应,而对大肠埃希菌、禽多杀性巴氏杆菌、空肠弯曲杆菌等14株其他病原微生物没有扩增反应;该LAMP最低检出量为0.17pg/反应,敏感性远高于常规PCR的56pg/反应。结果表明,所建立的副鸡禽杆菌LAMP具有快速、高效、方便操作等特点,适用于基层兽医部门进行副鸡禽杆菌的快速检测。
