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Analysis of arecoline in Semen Arecae decoction pieces by microchip capillary electrophoresis with contactless conductivity detection 被引量:2
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作者 Zi-You Cai Yong-Chong Li +1 位作者 Li-Hua Li Zuan-Guang Chen 《Journal of Pharmaceutical Analysis》 CAS 2012年第5期356-360,共5页
A new method for the determination of arecoline in Semen Arecae decoction pieces by microchip capillary electrophoresis with contactless conductivity detection (MCE-CCD) was proposed. The effects of various electrop... A new method for the determination of arecoline in Semen Arecae decoction pieces by microchip capillary electrophoresis with contactless conductivity detection (MCE-CCD) was proposed. The effects of various electrophoretic operating parameters on the analysis of arecoline were studied. Under the optimal conditions, arecoline was rapidly separated and detected in 1 rain with good linearity over the concentration range of 20 1500 uM (r2=0.9991) and the detection limit of 5 uM (S/N=3). The method was used for the analysis of arecoline satisfactorily with a recovery of 96.8 -104%. 展开更多
关键词 Microchip capillary electrophoresis Contactless conductivity detection ARECOLINE Semen arecae
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First Report of Phomopsis Leaf Spot on Patchouli [Pogostemon cablin( Blanco) Benth. ]Caused by Diaporthe arecae in China 被引量:1
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作者 Qingqian ZENG Hanqian HUANG +3 位作者 Zhouxi LEI Yicheng HUANG Yong HUANG Huiyun FAN 《Agricultural Biotechnology》 CAS 2020年第2期14-15,共2页
A severe leaf spot disease on patchouli,was observed in Guangdong,China.Early symptom appeared as circular or irregularly shaped and dark brown spots along the leaf margins and tips.As the disease progressed,the spots... A severe leaf spot disease on patchouli,was observed in Guangdong,China.Early symptom appeared as circular or irregularly shaped and dark brown spots along the leaf margins and tips.As the disease progressed,the spots enlarged and with masses black,sessile conidiomata were observed in lesions.The results of morphology,molecular biology and pathogenicity test indicate that the causal agent of leaf disease of patchouli is Diaporthe arecae. 展开更多
关键词 Patchouli PHOMOPSIS LEAF SPOT DIAPORTHE arecae
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The phytoplasma(Candidatus Phytoplasma arecae)is the crucial pathogen to cause areca palm yellow leaf disease
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作者 Shuangwei Song Qian Wang +10 位作者 Liangxiao Huo Liqiang Xie Jiaqi Chen Hongguang Cui Zhaoji Dai Jinrui Kang Yi Li Wei Guo Jinfeng Chen Le Kang Xiaoming Zhang 《Science Bulletin》 2025年第6期847-851,共5页
The areca palm(Areca catechu L.)is a vital tropical forest species widely utilized for vegetation restoration,landscaping,and greening purposes.Its nuts hold significant importance in traditional medicine,serving as r... The areca palm(Areca catechu L.)is a vital tropical forest species widely utilized for vegetation restoration,landscaping,and greening purposes.Its nuts hold significant importance in traditional medicine,serving as remedies for various ailments such as parasitic infections,digestive disorders,and depression[1–3].Areca palm yellow leaf disease(AYLD)poses a serious threat to areca palm growth. 展开更多
关键词 Areca palm yellow leaf disease Candidatus phytoplasma arecae traditional medicineserving PHYTOPLASMA areca palm areca palm areca parasitic infectionsdigestive disordersand Areca catechu
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槟榔仁对KM小鼠的急性毒性研究 被引量:7
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作者 刘书伟 王燕 +2 位作者 胡劲召 赵牧秋 史云峰 《湖北农业科学》 2015年第18期4532-4534,4543,共4页
为研究口服槟榔(Areca catechu Linnaeus)仁汁液的毒性,进行了小鼠的经口急性毒性试验。根据预试验确定正式试验给药剂量的范围,选用KM小鼠经口灌胃,一次给药,给药后连续观察7 d,记录小白鼠的中毒反应及死亡情况,采用寇氏法计算LD50及... 为研究口服槟榔(Areca catechu Linnaeus)仁汁液的毒性,进行了小鼠的经口急性毒性试验。根据预试验确定正式试验给药剂量的范围,选用KM小鼠经口灌胃,一次给药,给药后连续观察7 d,记录小白鼠的中毒反应及死亡情况,采用寇氏法计算LD50及LD50的95%可信限。结果表明,高剂量给药组小白鼠出现中毒反应并死亡,解剖后检查发现主要原因是胃肠和肺部的病变,测得槟榔仁汁液的LD50及LD50的95%可信限分别为12.425 g/(kg.bw)、10.610~14.551 g/(kg.bw),表明槟榔仁汁液可以被认为是实际无毒物质。槟榔仁原液干物质的LD50及LD50的95%可信限分别为1.349 g/(kg.bw)、1.152~1.580 g/(kg.bw),属于低毒物质。 展开更多
关键词 槟榔(Areca CATECHU Linnaeus)仁 小鼠 急性毒性 半数致死量
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食用槟榔切片机的设计 被引量:7
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作者 陈阳 陈致水 《湖北农业科学》 北大核心 2012年第16期3615-3618,共4页
目前槟榔(Areca catechu L.)的切片主要依靠手工操作,加工效率低下,产业得不到很好发展,究其原因是槟榔的果肉纤维与种子的连接层致密,分离很困难。设计了一台将槟榔果肉均匀切片,并尽可能保持种子完好,且能实现种子果肉与种子自动分离... 目前槟榔(Areca catechu L.)的切片主要依靠手工操作,加工效率低下,产业得不到很好发展,究其原因是槟榔的果肉纤维与种子的连接层致密,分离很困难。设计了一台将槟榔果肉均匀切片,并尽可能保持种子完好,且能实现种子果肉与种子自动分离的切片机,重点分析了关键部件及其参数。槟榔切片机的成功应用可有效解决槟榔收获后的加工问题,提高中国槟榔主产区农民经济收入和种植槟榔的积极性,促进当地槟榔产业的发展。 展开更多
关键词 槟榔(Areca CATECHU L.) 切片机 设计
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2015年槟榔产业发展报告及形势预测 被引量:16
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作者 黄慧德 《世界热带农业信息》 2017年第1期31-39,共9页
槟榔(Areca catechu L.)为棕榈科(Palmaceae)槟榔属(Areca)常绿乔木,原生于马来半岛的热带雨林中,是一种典型的热带植物,主要分布于亚洲与美洲的热带地区,主要生产国家有印度、斯里兰卡、菲律宾、缅甸、巴基斯坦、马来西亚、
关键词 槟榔属 黄化病 形势预测 ARECA 屯昌县 CATECHU 保亭县 产业发展报告 琼中 乐东
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槟榔农业生产技术研究 被引量:5
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作者 符之学 刘立云 +1 位作者 李艳 黄丽云 《安徽农业科学》 CAS 2014年第14期4229-4230,4292,共3页
槟榔被列为4大南药之首,前景广阔。该文概述了槟榔的产业概况,着重介绍了目前槟榔种植的品种及种苗生产、园地规划、园间管理以及病虫害防治方面存在的主要问题,并且提出了槟榔农业技术推广的具体化建议,为槟榔产业的生产和发展奠定了... 槟榔被列为4大南药之首,前景广阔。该文概述了槟榔的产业概况,着重介绍了目前槟榔种植的品种及种苗生产、园地规划、园间管理以及病虫害防治方面存在的主要问题,并且提出了槟榔农业技术推广的具体化建议,为槟榔产业的生产和发展奠定了一定的基础。 展开更多
关键词 槟榔(Areca CATECHU L ) 农业生产 技术推广
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PVX介导的槟榔坏死环斑病毒CP蛋白在烟草中的表达和纯化 被引量:2
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作者 孙迪 沈文涛 +5 位作者 庹德财 唐庆华 言普 黎小瑛 李娟玲 周鹏 《分子植物育种》 CAS 北大核心 2021年第10期3306-3313,共8页
槟榔坏死环斑病毒(areca palm necrotic ringspot virus,ANRSV)是新发现的一种槟榔黄化病致病病毒。本研究利用马铃薯X病毒(potato virus X,PVX)表达载体pgR107,在烟草中表达ANRSV的外壳蛋白(coat protein,CP),旨在分离纯化该蛋白并开... 槟榔坏死环斑病毒(areca palm necrotic ringspot virus,ANRSV)是新发现的一种槟榔黄化病致病病毒。本研究利用马铃薯X病毒(potato virus X,PVX)表达载体pgR107,在烟草中表达ANRSV的外壳蛋白(coat protein,CP),旨在分离纯化该蛋白并开展对病毒蛋白的功能研究和利用该蛋白制备多克隆抗体。为方便纯化,将8肽Strep蛋白标签序列融合到ANRSV-CP编码基因的3’端,利用In-Fusion无缝克隆策略将其克隆到PVX载体,构建重组表达质粒PVX-ANRSV-CP-Strep。采用电转化法将质粒PVX-ANRSV-CP-Strep转入根瘤农杆菌GV3101中,通过农杆菌介导注射侵染接种本氏烟,14 d后对收集的16 g表达ANRSV-CP-Strep蛋白、PVX侵染的本生烟草样品进行了蛋白提取,采用SDS-PAGE和Western blot检测ANRSV-CP-Strep蛋白的表达情况。测序结果显示植物病毒表达载体PVX-ANRSV-CP-Strep构建成功。经Strep-Tactin■XT High Capacity亲和层析柱纯化,SDS-PAGE和Western blot检测结果显示ANRSV-CP-Strep重组蛋白可通过PVX载体在烟草中有效表达,其表达量为22μg/g(鲜叶质量)。本研究为后续利用该蛋白制备多克隆抗体并建立ELISA方法进行病毒检测提供了理论依据。 展开更多
关键词 槟榔坏死环斑病毒(Areca palm necrotic ringspot virus) 真核表达 载体构建 蛋白纯化
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产地和果形对槟榔主要活性成分及抗氧化活性的影响 被引量:5
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作者 陈明洋 卓小垒 +3 位作者 代佳妮 于靖 戚华沙 吴友根 《热带生物学报》 2020年第1期31-41,共11页
为了系统研究海南不同产地及果形槟榔的主要活性成分及抗氧化活性,采用比色法测定成熟槟榔青皮和种子的总生物碱和总黄酮含量,采用DPPH,ABTS和FRAP法测定其抗氧化能力,利用SPSS软件进行主成分分析和聚类分析,采用TOPSIS法进行综合评价... 为了系统研究海南不同产地及果形槟榔的主要活性成分及抗氧化活性,采用比色法测定成熟槟榔青皮和种子的总生物碱和总黄酮含量,采用DPPH,ABTS和FRAP法测定其抗氧化能力,利用SPSS软件进行主成分分析和聚类分析,采用TOPSIS法进行综合评价。结果表明:不同产地的成熟槟榔,其形态指标及抗氧化能力明显不同,TOPSIS综合排序显示白石岭4(BSL4)的抗氧化能力最强;果实越饱满的槟榔,ABTS自由基清除能力越强。 展开更多
关键词 槟榔(Areca catechu L.) 抗氧化能力 主成分分析 聚类分析
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槟榔原生质体分离及瞬时转化体系的建立 被引量:1
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作者 曹春艳 王威 +1 位作者 杨新奇 李东栋 《分子植物育种》 CAS 北大核心 2023年第17期5730-5737,共8页
为建立一套稳定高效的槟榔叶肉细胞原生质体分离及瞬时转化方法,本研究采用酶解法以槟榔嫩花苞、嫩叶片、胚状体为实验材料,对影响其原生质体产率、活力及瞬时转化效率的因素进行了研究。结果表明:槟榔花苞在酶解4 h产量和活力最高,产量... 为建立一套稳定高效的槟榔叶肉细胞原生质体分离及瞬时转化方法,本研究采用酶解法以槟榔嫩花苞、嫩叶片、胚状体为实验材料,对影响其原生质体产率、活力及瞬时转化效率的因素进行了研究。结果表明:槟榔花苞在酶解4 h产量和活力最高,产量为9.1×10~7个/mL,活力为92%。槟榔原生质体瞬时转化中原生质体/质粒与40%PEG-4000水浴(45℃)30 min、室温PEG孵育40 min的条件下,利用PEG介导法将表达绿色荧光蛋白的质粒P35-EGFP转入槟榔原生质体中,转化效率达到13%。通过共聚焦显微镜观察和荧光定量分析证明,EGFP与MYB108都成功被过表达。该研究建立了槟榔叶肉原生质体的分离及瞬时转化方法,有较好的应用价值。可以用作蛋白质亚细胞定位和基因调控作用的研究,后续还可以被用作验证蛋白质与蛋白质或蛋白质与DNA的互作等研究。 展开更多
关键词 槟榔(Areca catechu) 原生质体 瞬时表达系统 亚细胞定位 基因功能
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槟榔防虫增产及无公害防虫新技术的研究 被引量:2
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作者 樊瑛 甘炳春 +4 位作者 陈■亮 薛健 陈伟平 任建联 陈良德 《医学研究杂志》 1991年第9期31-32,共2页
槟榔Areca catechu L.为棕榈科木本植物,以果入药,有健胃、驱虫、行水、下气、宽中作用,是我国重要南药之一。槟榔除药用外还是南方群众喜食和馈赠之佳品,主产于海南省。由于槟榔栽培技术比较落后,虫害严重,无施肥习惯,一般产量较低且... 槟榔Areca catechu L.为棕榈科木本植物,以果入药,有健胃、驱虫、行水、下气、宽中作用,是我国重要南药之一。槟榔除药用外还是南方群众喜食和馈赠之佳品,主产于海南省。由于槟榔栽培技术比较落后,虫害严重,无施肥习惯,一般产量较低且不稳定,国产槟榔远不能满足市场需要,致使每年花外汇进口槟榔以供药用。因此研究解决槟榔栽培技术、提高其产量和质量,促进槟榔生产的发展,以满足人民药用和生活需要,具有重要意义。调查研究表明,影响槟榔产量的重要因素是虫害严重。据不完全统计,每年因虫害造成的损失,一般为20%~30%, 展开更多
关键词 CATECHU ARECA 南药 施肥习惯 虫害防治 栽培技术 满足市场需要 红脉穗螟 幼虫死亡率 总生物碱
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Effect of areca on contraction of colonic muscle strips in rats 被引量:20
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作者 Dong-Ping Xie Lian-Bi Chen,Department of Physiology,Medical College,Shandong University,Jinan 250012,Shandong Province,China Wei Li Song-Yi Qu Tian-Zhen Zheng,Department of Physiology,Lanzhou Medical College,Lanzhou 730000,Gansu Province,China Ying-Li Yang,Northwest Normal University,Lanzhou 730070,Gansu Province,China Yong-Hui Ding Yu-Ling Wei,Drug Control Institute of Gansu Province,Lanzhou 730000,Gansu Province,China 《World Journal of Gastroenterology》 SCIE CAS CSCD 2002年第2期350-352,共3页
AIM: To investigate the effects of areca on the contractile activity of isolated colonic muscle strips in rats and mechanism involved. METHODS: Each strip (LMPC, longitudinal muscle of proximal colon; CMPC, circular m... AIM: To investigate the effects of areca on the contractile activity of isolated colonic muscle strips in rats and mechanism involved. METHODS: Each strip (LMPC, longitudinal muscle of proximal colon; CMPC, circular muscle of proximal colon; LMDC, longitudinal muscle of distal colon; CMDC, circular muscle of distal colon.) was suspended in a tissue chamber containing 5 mL Krebs solution (37 degrees C), bubbled continuously with 950 mL.L(-1) O(2) and 50 mL.L(-1) CO(2). The mean contractile amplitude (A), the resting tension (T), and the contractile frequency (F) were simultaneously recorded on recorders. RESULTS: Areca dose dependently increased the mean contractile amplitude, the resting tension of proximal and distal colonic smooth muscle strips in rats (P【0.05). It also partly increased the contractile frequency of colonic smooth muscle strips in rats (P【0.05). The effects were partly inhibited by atropine (the resting tension of LMPC decreased from 0.44 +/- 0.12 to 0.17 +/- 0.03; the resting tension of LMDC decreased from 0.71 +/- 0.14 to 0.03 +/- 0.01; the mean contractile amplitude of LMPC increased from -45.8 +/- 7.2 to -30.5 +/- 2.9; the motility index of CMDC decreased from 86.6 +/- 17.3 to 32.8 +/- 9.3; P【0.05 vs areca), but the effects were not inhibited by hexamethonium (P】0.05). CONCLUSION: Areca stimulated the motility of isolated colonic smooth muscle strips in rats. The stimulation of areca might be relevant with M receptor partly. 展开更多
关键词 ARECA Animals ATROPINE Colon Female Hexamethonium In Vitro Male Muscle Contraction Nicotinic Antagonists Parasympatholytics Plant Extracts RATS Rats Wistar Research Support Non-U.S. Gov't
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Antioxidant and Cytotoxic Phenolic Compounds of Areca Nut(Areca catechu) 被引量:5
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作者 ZHANG Xing WU Jiao HAN Zhuang MEI Wen-li DAI Hao-fu 《Chemical Research in Chinese Universities》 SCIE CAS CSCD 2010年第1期161-164,共4页
Areca catechu L.(Palmae), commonly known as an important economical seed crop, is widely culti- vated in tropical and subtropical areas, including India, Southeast Asia, East Africa and New Guinea. Areca nut(freque... Areca catechu L.(Palmae), commonly known as an important economical seed crop, is widely culti- vated in tropical and subtropical areas, including India, Southeast Asia, East Africa and New Guinea. Areca nut(frequently known as betel nut) is the ripe fruit of the tree A. catechu. Areca nut can be chewed and it is a common masticatory in tropical and subtropical countries. It was estimated in the early 1990s that 10% to 20% of the world's population chewed betel quid daily. Areca nut is commonly used in folklore medicine for treatment of various diseases such as dyspep sia, constipation, beriberi and oedema. 展开更多
关键词 Areca catechu PHENYLPROPANOID DPPH Antioxidant activity Cytotoxic activity
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Influence of Boric Acid and Sucrose on the Germination and Growth of Areca Pollen 被引量:3
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作者 Liyun Liu Liyun Huang Yan Li 《American Journal of Plant Sciences》 2013年第8期1669-1674,共6页
This study investigates the effect of boric acid and sucrose on the germination and growth of Areca catechu L (areca palm). Using areca pollen from Hainan, China, this study determines the influence of culture media w... This study investigates the effect of boric acid and sucrose on the germination and growth of Areca catechu L (areca palm). Using areca pollen from Hainan, China, this study determines the influence of culture media with different concentrations of boric acid and sucrose on the germination and growth of areca pollen. Results show that the germination and growth of the pollen tube growth are highest under a boric acid concentration of 0.4 g/L to 0.6 g/L. The germination rate of areca pollen is highest in solid medium with a sucrose concentration of 40 g/L. The optimum culture medium for developing areca pollen comprises 5 g/L agar, 0.4 g/L to 0.6 g/L boric acid, 40 g/L sucrose, and water. Furthermore, areca pollen grows most rapidly approximately 1.5 h after in vitro cultivation at normal temperature, and growth stabilizes at approximately 3 h. The principle that governs the development of areca pollen is also discussed. 展开更多
关键词 Boric ACID SUCROSE ARECA NUT POLLEN GERMINATION
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Dyeing of Polyester and Nylon with Semi-synthetic Azo Dye by Chemical Modification of Natural Source Areca Nut 被引量:2
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作者 Ashitosh B.Pawar Sandeep P.More R.V.Adivarekar 《Natural Products and Bioprospecting》 CAS 2018年第1期23-29,共7页
Various azo compounds(Modified dyes)have been synthesised by chemical modification of areca nut extract(epicate-chin),a plant-based Polyphenolic compound to get semi-synthetic dyes.Three different primary amines namel... Various azo compounds(Modified dyes)have been synthesised by chemical modification of areca nut extract(epicate-chin),a plant-based Polyphenolic compound to get semi-synthetic dyes.Three different primary amines namely p-nitro aniline,p-anisidine and aniline,were diazotized to form their corresponding diazonium salts which were further coupled with an areca nut extract.Preliminary characterization of the areca nut extract and the resultant azo compounds(Modified dyes)was carried out in terms of melting point,solubility tests,thin layer chromatography,UV-Visible and FTIR spectroscopy.These modified dyes were further applied on polyester and nylon fabrics and%dye exhaustion was evaluated.Dyed fabrics were further tested for their fastness properties such as wash fastness,rubbing fastness,light fastness and sublimation fastness.The results of the fastness tests indicate that,all the three modified dyes have good dyeability for polyester and nylon fabrics.The dyed fabrics were also tested for ultraviolet protection factor which showed very good ultraviolet protection. 展开更多
关键词 Semi-synthetic azo dye Chemical modification Modified dyes Areca nut Ultraviolet protection factor
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Chewing substances with or without tobacco and risk of cardiovascular disease in Asia:a meta-analysis 被引量:1
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作者 Li-na ZHANG Yun-mei YANG +2 位作者 Zhe-rong XU Qi-feng GUI Qin-qing HU 《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 SCIE CAS CSCD 2010年第9期681-689,共9页
Objective:To assess whether people who ever use any form of chewing substance in Asia are at increased risk of cardiovascular disease(CVD).Methods:PubMed and ISI Web of Science were searched for relevant studies,with ... Objective:To assess whether people who ever use any form of chewing substance in Asia are at increased risk of cardiovascular disease(CVD).Methods:PubMed and ISI Web of Science were searched for relevant studies,with no limitation on language or study year.Studies were included if they provided quantitative estimate of the association between ever use of chewing substance and the occurrence of CVD.Two authors independently implemented inclusion criteria,abstracted study characteristics,and performed meta-analysis.Summary relative risks were estimated on the basis of a random effect model.We used Q statistic and Egger's test to examine heterogeneity across studies and potential publication bias,respectively.Results:Eight eligible studies were included.The relative risk of CVD for ever using chewing substances with or without tobacco was 1.26(95% confidence interval(CI) 1.12-1.40),which was unchanged when restricted to cohort studies [1.25(1.08-1.42)] or cohort studies in Taiwan [1.31(1.12-1.51)].The summary relative risk for ischemic heart disease was 1.27(1.02-1.52),and was lowered to 1.26(0.85-1.67) after exclusion of a cross-sectional study.The overall relative risk for cerebrovascular disease was 1.32(1.08-1.56).On the basis of the Taiwan data,the summary relative risk of CVD for betel(Areca catechu) chewing was 1.30(1.17-1.44).Data on dose-response were limited to betel chewing in Taiwan,suggesting a relationship between risk of CVD and cumulative exposure.Two large cohorts in Taiwan reported a greater risk of CVD with betel chewing than with smoking.Conclusions:An association was detected between betel chewing with or without tobacco and the risk of CVD.Betel chewing may impose a greater CVD risk than smoking.More effort is needed in developing betel chewing cessation programmes.The relationship between betel chewing and subgroups of CVD requires further investigation. 展开更多
关键词 Cardiovascular disease Areca catechu Betel quid Chewing tobacco Relative risk META-ANALYSIS
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槟榔高产育种的理想株型设计
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作者 黄昭奋 董峰 +1 位作者 郦建权 方富炯 《分子植物育种》 CAS CSCD 2009年第4期763-766,共4页
本研究通过对槟榔高产株型的分析和育种栽培的实践,提出了"基于理想株型设计的槟榔高产育种"思路。理想株的叶片呈明显披垂型,与主杆间的夹角为70.5°±3.35°,这种叶片的空间分布可能有利于阳光的吸收。理想株... 本研究通过对槟榔高产株型的分析和育种栽培的实践,提出了"基于理想株型设计的槟榔高产育种"思路。理想株的叶片呈明显披垂型,与主杆间的夹角为70.5°±3.35°,这种叶片的空间分布可能有利于阳光的吸收。理想株的第一苞发育节位数和初始节位高度显著较低,在第6节至第7节开始苞的分化和发育,初始节位高度为45cm左右。理想株的果穗分枝数在14个左右,与其它株型基本一样,但果穗小花数为179±19.9个,显著高于其它株型。理想型株型植株矮小,节间长度较短,叶片较大,散状披垂,展开角度较大,有利于接受更多的光照,进行光合作用,产生更多有机物。 展开更多
关键词 槟榔(Areca CATECHU L.) 理想株型 高产育种
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槟榔DUR3基因鉴定及组织表达特异性分析
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作者 曹皓玮 周广振 李佳 《分子植物育种》 CAS 北大核心 2022年第23期7729-7736,共8页
为了探究DUR3家族基因在槟榔尿素转运过程中的作用,本研究利用拟南芥DUR3同源蛋白为探针序列,从槟榔基因组数据库中鉴定DUR3家族基因,利用生物信息学方法对其基因结构、进化关系、保守结构域、跨膜结构域和表达模式进行分析。结果表明,... 为了探究DUR3家族基因在槟榔尿素转运过程中的作用,本研究利用拟南芥DUR3同源蛋白为探针序列,从槟榔基因组数据库中鉴定DUR3家族基因,利用生物信息学方法对其基因结构、进化关系、保守结构域、跨膜结构域和表达模式进行分析。结果表明,从槟榔基因组中共鉴定出2个DUR3基因,与其他植物的DUR3同源蛋白类似,具有15个跨膜结构域,其保守结构域和基序位置也高度相似。不同物种的DUR3氨基酸序列构建的进化树显示,双子叶植物和禾本科单子叶植物各聚为一支,而棕榈科单子叶的植物单独聚为一支,且与双子叶植物亲缘关系更近。DUR3直系同源基因的Ka/Ks比值小于1,这表明这些物种的DUR3基因在物种进化中受到纯化选择压力。本研究为深入研究槟榔的DUR3基因的功能提供了理论依据。 展开更多
关键词 槟榔(Areca cat) DUR3 生物信息学
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