Objective To analyze the relationship between polymorphism at the Apolipoprotein AI (Apo AI) gene and the risk for coronary artery disease. Methods A total of 107 patients (mean age 56 ±11 years) diagnosed as hav...Objective To analyze the relationship between polymorphism at the Apolipoprotein AI (Apo AI) gene and the risk for coronary artery disease. Methods A total of 107 patients (mean age 56 ±11 years) diagnosed as having stable angina pectoris (SAP) (23 cases), unstable angina pectoris (UAP) (23 cases) or myocardial infarction (MI) (61 cases) were prospectively evaluated. DNA was obtained from the 107 patients and 50 controls. In order to determine the Apo AI genotypes at two polymorphic sites (G/A at -75 bp, and C/T at+83 bp), DNA was PCR amplified and digested with MspI. Results The frequency of carriers of the rare allele at the - 75 bp site (M1-) was 0.49 in cases and 0.30 in controls (P<0. 05). The frequencies of the M1-allele among patients with SAP, UAP, MI and controls were 0. 37 (vs. controls, P > 0. 05), 0.54 (vs. controls, P < 0.05), 0.52 (vs. controls, P<0. 05) and 0. 30, respectively. The frequencies for carriers of the rare allele at the + 83bp polymorphism (M2) were observed among patients with SAP (0. 09, vs. controls, P > 0.05), UAP (0.11, vs. controls, P>0.05) or MI (0. 12, vs. controls, P>0. 05) and controls (0. 12). There was an slightly increase in the frequency of the Ml - allele in patients with SAP to UAP or MI (0. 37 vs. 0. 54 vs. 0. 52; all P>0. 05) and Ml polymorphism as a risk factor for CAD ( OR = 3. 74, P < 0. 05). In the + 83bp polymorphism there was no difference in the allelelic frequencies in cases and controls (0. 11 vs. 0. 12; P > 0. 05). There was no significantdifference in the frequency of the M2 - allele in patients with SAP to UAP or MI (0.09 vs. 0. 11 vs. 0. 12; all P>0. 05) and M2 polymorphism not as a factor for CAD (OR=0.80, P>0. 05).Plasma lipoprotein values in patients with the allele M1-and M2 - had no different levels than those homozygous for the M1+and M2+(P>0.05). Conclusion Ml polymorphism (M1 - ) may be as a risk factor for CAD and M2 polymorphism (M2 - ) not as a factor for CAD in Chinese Xinjiang Uygur and Han population.展开更多
To investigate the influence on plasma lipid levels of alcohol and a common polymorphism in the human apolipoprotein AI gene promoter at a position 75?bp upstream of the transcription start site Methods For this...To investigate the influence on plasma lipid levels of alcohol and a common polymorphism in the human apolipoprotein AI gene promoter at a position 75?bp upstream of the transcription start site Methods For this study, 742 healthy Yi and Han subjects all above 15 years old formed the total population which was divided into three groups: the Yi farmer group, the Yi emigrant group and the Han resident group All estimates of plasma lipids and apolipoproteins were performed using an auto analyzer Genetic DNA was prepared from the blood clots using the Triton X 100 lysis technique Amplification of a 432?bp fragment of the apoAI gene promoter was performed using PCR followed by restriction digestion, electrophoresis and identification of the genotypes involved Results The samples were divided on the basis of alcohol consumption: non drinkers, 1-25?g/day, 26-75?g/day and >75?g/day Comparing the four alcohol consumption groups, plasma HDLC and apoAI levels were increased as the alcohol consumption increased with no evidence of threshold effects in the Yi farmers and the Han people groups A similar association was found in the Yi emigrant group, but was not statistically significant The frequencies of the A allele in the three populations were similar, and no significant difference of lipid and apolipoprotein levels was found between subjects with and without the A allele in the three populations But, in Han and Yi emigrant samples, the drinkers with the GG genotype had higher plasma HDLC and apoAI levels than non drinkers with the same genotype, while the drinkers with the A allele had lower plasma HDLC and apoAI levels than drinkers without the A allele Non drinkers with the A allele had higher levels of apoAI than non drinkers with GG genotypes It was estimated that 18% of the variability of plasma apoAI level could be explained by the G to A polymorphism in non drinkers of Yi emigrants ( F =8 94, P <0 01) Conclusions The present data suggest that moderate alcohol consumption and the G to A substitution could lower the risk of coronary heart disease (CHD), but the beneficial effects of one will be negated by the other展开更多
It has been suggested by many researchers that the dangers of developing atheroclerosis and the coronary heart disease in human race are closely correlated with the amount of high density lipoproteins in plasma. High ...It has been suggested by many researchers that the dangers of developing atheroclerosis and the coronary heart disease in human race are closely correlated with the amount of high density lipoproteins in plasma. High density lipoprotein is capable of delivering cholesterol from the peripheral tissues to the liver for disposal and therefore prevent the accumulation of cholesterol in the artery wall. Apolipoprotein AI (apoAI) is the major polypeptide of high density lipoproteins and seems to play an important role in this cholesterol transport.展开更多
目的:研究载脂蛋白AI、B(ApoAI、B)基因多态性与非创伤性股骨头坏死(nontraumatic osteonecrosis of femoral head,NONFH)中医证候的相关性。方法:采用聚合酶链式反应(PCR)和限制性片段长度多态性(RFLP)对143例NON-FH患者和92例正常人...目的:研究载脂蛋白AI、B(ApoAI、B)基因多态性与非创伤性股骨头坏死(nontraumatic osteonecrosis of femoral head,NONFH)中医证候的相关性。方法:采用聚合酶链式反应(PCR)和限制性片段长度多态性(RFLP)对143例NON-FH患者和92例正常人分别进行ApoAI基因启动子-75bp位点、内含子+83bp位点,ApoB基因EcoRI、XbaI的多态性研究,采用聚合酶链反应(PCR)和琼脂糖凝胶电泳分析ApoB基因3’端可变数目串联重复序列(3’-VNTR);对NONFH患者按痰瘀阻络、经脉痹阻和肝肾亏虚3个证型进行中医辨证,用SAS软件分析基因型、等位基因在NONFH证候分型出现频率。结果:ApoAI启动子-75bp基因位点,经脉痹阻证和肝肾亏虚证的A/A基因型频率均高于对照组(P<0.05),肝肾亏虚证的G/A基因型频率显著低于对照组(P<0.01)、痰瘀阻络证和经脉痹阻证(P<0.05),而G/G基因型频率明显高于经脉痹阻证(P<0.05);等位基因比较中,经脉痹阻证G等位基因频率显著低于其他各组(P<0.05)。ApoAI启动子-内含子中,与痰瘀阻络相比,经脉痹阻证组的G/G-C/C、G/A-C/C基因型频率均显著降低(P<0.05);肝肾亏虚证组G/A-C/C基因型频率显著降低(P<0.05);而且肝肾亏虚证组G/A-C/C基因型频率显著低于经脉痹阻证组。ApoB基因XbaI基因型中,经脉痹阻证组X+X-基因型频率明显高于对照组(P<0.05),但X+、X-等位基因频率无明显差异(P>0.05)。在ApoAI基因内含子+83bp位点、ApoB基因EcoRI基因型、3’-VNTR基因型频率、等位基因频率在各证型及对照组间分布无明显差异(P>0.05)。结论:ApoAI基因启动子-75bp位点多态性、启动子——内含子位点多态性及ApoB基因XbaI基因型与NONFH证候分型有一定相关性;ApoAI基因内含子+83bp位点、ApoB基因EcoRI、3’-VNTR多态性可能与NONFH的证候发生、发展无相关关系。展开更多
文摘Objective To analyze the relationship between polymorphism at the Apolipoprotein AI (Apo AI) gene and the risk for coronary artery disease. Methods A total of 107 patients (mean age 56 ±11 years) diagnosed as having stable angina pectoris (SAP) (23 cases), unstable angina pectoris (UAP) (23 cases) or myocardial infarction (MI) (61 cases) were prospectively evaluated. DNA was obtained from the 107 patients and 50 controls. In order to determine the Apo AI genotypes at two polymorphic sites (G/A at -75 bp, and C/T at+83 bp), DNA was PCR amplified and digested with MspI. Results The frequency of carriers of the rare allele at the - 75 bp site (M1-) was 0.49 in cases and 0.30 in controls (P<0. 05). The frequencies of the M1-allele among patients with SAP, UAP, MI and controls were 0. 37 (vs. controls, P > 0. 05), 0.54 (vs. controls, P < 0.05), 0.52 (vs. controls, P<0. 05) and 0. 30, respectively. The frequencies for carriers of the rare allele at the + 83bp polymorphism (M2) were observed among patients with SAP (0. 09, vs. controls, P > 0.05), UAP (0.11, vs. controls, P>0.05) or MI (0. 12, vs. controls, P>0. 05) and controls (0. 12). There was an slightly increase in the frequency of the Ml - allele in patients with SAP to UAP or MI (0. 37 vs. 0. 54 vs. 0. 52; all P>0. 05) and Ml polymorphism as a risk factor for CAD ( OR = 3. 74, P < 0. 05). In the + 83bp polymorphism there was no difference in the allelelic frequencies in cases and controls (0. 11 vs. 0. 12; P > 0. 05). There was no significantdifference in the frequency of the M2 - allele in patients with SAP to UAP or MI (0.09 vs. 0. 11 vs. 0. 12; all P>0. 05) and M2 polymorphism not as a factor for CAD (OR=0.80, P>0. 05).Plasma lipoprotein values in patients with the allele M1-and M2 - had no different levels than those homozygous for the M1+and M2+(P>0.05). Conclusion Ml polymorphism (M1 - ) may be as a risk factor for CAD and M2 polymorphism (M2 - ) not as a factor for CAD in Chinese Xinjiang Uygur and Han population.
文摘To investigate the influence on plasma lipid levels of alcohol and a common polymorphism in the human apolipoprotein AI gene promoter at a position 75?bp upstream of the transcription start site Methods For this study, 742 healthy Yi and Han subjects all above 15 years old formed the total population which was divided into three groups: the Yi farmer group, the Yi emigrant group and the Han resident group All estimates of plasma lipids and apolipoproteins were performed using an auto analyzer Genetic DNA was prepared from the blood clots using the Triton X 100 lysis technique Amplification of a 432?bp fragment of the apoAI gene promoter was performed using PCR followed by restriction digestion, electrophoresis and identification of the genotypes involved Results The samples were divided on the basis of alcohol consumption: non drinkers, 1-25?g/day, 26-75?g/day and >75?g/day Comparing the four alcohol consumption groups, plasma HDLC and apoAI levels were increased as the alcohol consumption increased with no evidence of threshold effects in the Yi farmers and the Han people groups A similar association was found in the Yi emigrant group, but was not statistically significant The frequencies of the A allele in the three populations were similar, and no significant difference of lipid and apolipoprotein levels was found between subjects with and without the A allele in the three populations But, in Han and Yi emigrant samples, the drinkers with the GG genotype had higher plasma HDLC and apoAI levels than non drinkers with the same genotype, while the drinkers with the A allele had lower plasma HDLC and apoAI levels than drinkers without the A allele Non drinkers with the A allele had higher levels of apoAI than non drinkers with GG genotypes It was estimated that 18% of the variability of plasma apoAI level could be explained by the G to A polymorphism in non drinkers of Yi emigrants ( F =8 94, P <0 01) Conclusions The present data suggest that moderate alcohol consumption and the G to A substitution could lower the risk of coronary heart disease (CHD), but the beneficial effects of one will be negated by the other
基金Project supported by the National Natural Science Foundation of China.
文摘It has been suggested by many researchers that the dangers of developing atheroclerosis and the coronary heart disease in human race are closely correlated with the amount of high density lipoproteins in plasma. High density lipoprotein is capable of delivering cholesterol from the peripheral tissues to the liver for disposal and therefore prevent the accumulation of cholesterol in the artery wall. Apolipoprotein AI (apoAI) is the major polypeptide of high density lipoproteins and seems to play an important role in this cholesterol transport.
文摘目的:研究载脂蛋白AI、B(ApoAI、B)基因多态性与非创伤性股骨头坏死(nontraumatic osteonecrosis of femoral head,NONFH)中医证候的相关性。方法:采用聚合酶链式反应(PCR)和限制性片段长度多态性(RFLP)对143例NON-FH患者和92例正常人分别进行ApoAI基因启动子-75bp位点、内含子+83bp位点,ApoB基因EcoRI、XbaI的多态性研究,采用聚合酶链反应(PCR)和琼脂糖凝胶电泳分析ApoB基因3’端可变数目串联重复序列(3’-VNTR);对NONFH患者按痰瘀阻络、经脉痹阻和肝肾亏虚3个证型进行中医辨证,用SAS软件分析基因型、等位基因在NONFH证候分型出现频率。结果:ApoAI启动子-75bp基因位点,经脉痹阻证和肝肾亏虚证的A/A基因型频率均高于对照组(P<0.05),肝肾亏虚证的G/A基因型频率显著低于对照组(P<0.01)、痰瘀阻络证和经脉痹阻证(P<0.05),而G/G基因型频率明显高于经脉痹阻证(P<0.05);等位基因比较中,经脉痹阻证G等位基因频率显著低于其他各组(P<0.05)。ApoAI启动子-内含子中,与痰瘀阻络相比,经脉痹阻证组的G/G-C/C、G/A-C/C基因型频率均显著降低(P<0.05);肝肾亏虚证组G/A-C/C基因型频率显著降低(P<0.05);而且肝肾亏虚证组G/A-C/C基因型频率显著低于经脉痹阻证组。ApoB基因XbaI基因型中,经脉痹阻证组X+X-基因型频率明显高于对照组(P<0.05),但X+、X-等位基因频率无明显差异(P>0.05)。在ApoAI基因内含子+83bp位点、ApoB基因EcoRI基因型、3’-VNTR基因型频率、等位基因频率在各证型及对照组间分布无明显差异(P>0.05)。结论:ApoAI基因启动子-75bp位点多态性、启动子——内含子位点多态性及ApoB基因XbaI基因型与NONFH证候分型有一定相关性;ApoAI基因内含子+83bp位点、ApoB基因EcoRI、3’-VNTR多态性可能与NONFH的证候发生、发展无相关关系。
