Hyperlipidemia has high morbidity. We explored the novel activities of six Chinese herbs before and after fermentation for preventing hyperlipidemia. The herbs were fermented with white rot fungi Ganoderma lucidum, a ...Hyperlipidemia has high morbidity. We explored the novel activities of six Chinese herbs before and after fermentation for preventing hyperlipidemia. The herbs were fermented with white rot fungi Ganoderma lucidum, a strain of EN2. Lovastatin was used as the positive control. Mice were treated with those herbs before and after fermentation treatment for 15 d and then injected peritoneally with Triton 3393. After the treatment, the serum total cholesterol （TC） and triglyceride （TG） were measured. More- over, the changes of the ingredients of those herbs were analyzed by fingerprint chromatography. The results showed that Herba leonuri before fermentation, Ramulus mori and Caulis lonicerae after fermentation, Radix sophoraeflavescentis before and after fermentation showed activities to prevent hypertriglyceridemia, while Ramulus mori and Herba leonuri before fermentation showed activities to prevent hypercholesterolemia. In addition, some new ingredients were appeared after fermentation. In conclusion, the novel activities of these herbs to prevent hyperlipidemia were identified and the new ingredients that emerged after fermentation warrant further exploration.展开更多
This study investigated hypolipidemic,weight-reducing,and hepatoprotective effects of Antrodia cinnamomea mycelial extract obtained from solid-state culture(ACME)in an HFr D-induced obese/hyperlipidemic mouse model.Fo...This study investigated hypolipidemic,weight-reducing,and hepatoprotective effects of Antrodia cinnamomea mycelial extract obtained from solid-state culture(ACME)in an HFr D-induced obese/hyperlipidemic mouse model.Following 4-week ACME treatment,body weight,epididymal fat index,and some serum biochemical indices were measured.Expression levels of some related genes involved in cholesterol and lipid metabolism were analyzed by q RT-PCR.Moreover,histological studies of hepatic tissues were also conducted.After ACME treatment,body weight and epididymal fat index were significantly lower than that in model control group.ACME and simvastatin significantly reduced serum total cholesterol(T-CHO)and low-density lipoprotein cholesterol(LDL-C)levels,and increased high-density lipoprotein cholesterol(HDL-C)level.Subsequent experiments showed that:(i)ACME regulated transcriptional expression of 3-hydroxy-3-methylglutaryl-Co A reductase(HMGR),low-density lipoprotein receptor(LDLR),adipose triglyceride lipase(ATGL),and fatty acid synthase(FAS),with consequent reduction of blood lipid levels and body weight;(ii)ACME enhanced total antioxidant capacity(T-AOC)and superoxide dismutase(SOD)activity in hepatic tissues;(iii)ACME reduced malondialdehyde(MDA)level and ameliorated lipid oxidative damage in liver.Our findings indicate that ACME is a strong candidate for development as a novel anti-hyperlipidemia and anti-obesity health product.展开更多
The objective of this study was to establish an efficient and low-cost method for preparing glucoraphenin(GRE)from stir-fried seeds of Raphanus sativus L.,and then evaluate the anti-hyperlipidemia activity of GRE.The ...The objective of this study was to establish an efficient and low-cost method for preparing glucoraphenin(GRE)from stir-fried seeds of Raphanus sativus L.,and then evaluate the anti-hyperlipidemia activity of GRE.The optimal resin was selected by comparing adsorption and desorption ability of six kinds of anion exchange resins for GRE.The loading and elution conditions of the optimal resin for GRE were then optimized by dynamic adsorption and desorption experiments.The results showed that D301-Cl^(-)resin was the optimal resin,and the optimal loading conditions were the original sample solution(pH=6)with a concentration of 0.10 g/mL,the sample loading flow rate was 2 BV/h,and the sample volume was 7 BV The optimum elution conditions were as follows:the elution solution was 5 g/100 mL NaCl,followed by subsequent elution with 4 BV at an elution flow rate of 2 BV/h.The collected eluent was further purified by ultrafiltration and preparative high-performance liquid chromatography(pHPLC)to obtain GRE,and the molecular weight and structure of GRE were determined by high performance liquid chromatography time-of-flight mass spectrometry and nuclear magnetic resonance,respectively.The purity of GRE was detected to be 95.1%by HPLC.Animal experiments showed that GRE had anti-hyperlipidemic effects by improving serum and liver lipid levels,alleviating liver lipid deposition,and protecting the liver.This study indicated that GRE may be a potential anti-hyperlipidemia drug candidate or dietary supplement.展开更多
对长白山红松籽油调节血脂作用进行研究。建立昆明小白鼠高脂血症模型后,观察红松籽油调节血脂功能。通过测定血清总胆固醇(total cholesterol,TC)、甘油三酯(triglycerides,TG)、高密度脂蛋白胆固醇(high density lipoprotein choleste...对长白山红松籽油调节血脂作用进行研究。建立昆明小白鼠高脂血症模型后,观察红松籽油调节血脂功能。通过测定血清总胆固醇(total cholesterol,TC)、甘油三酯(triglycerides,TG)、高密度脂蛋白胆固醇(high density lipoprotein cholesterol,HDL-C)、低密度脂蛋白胆固醇(low density lipoprotein cholesterol,LDL-C)、葡萄糖(glucose,Glu)含量以及肝脏和肾脏的TC、TG水平来检测长白山红松籽油的降血脂效应。结果表明:不同剂量的红松籽油均不同程度地降低高脂饲料对受试小白鼠高血脂症的诱导作用,显著改善肝脏及肾脏脂质的积累,红松籽油具有显著的降血脂效应。展开更多
文摘Hyperlipidemia has high morbidity. We explored the novel activities of six Chinese herbs before and after fermentation for preventing hyperlipidemia. The herbs were fermented with white rot fungi Ganoderma lucidum, a strain of EN2. Lovastatin was used as the positive control. Mice were treated with those herbs before and after fermentation treatment for 15 d and then injected peritoneally with Triton 3393. After the treatment, the serum total cholesterol （TC） and triglyceride （TG） were measured. More- over, the changes of the ingredients of those herbs were analyzed by fingerprint chromatography. The results showed that Herba leonuri before fermentation, Ramulus mori and Caulis lonicerae after fermentation, Radix sophoraeflavescentis before and after fermentation showed activities to prevent hypertriglyceridemia, while Ramulus mori and Herba leonuri before fermentation showed activities to prevent hypercholesterolemia. In addition, some new ingredients were appeared after fermentation. In conclusion, the novel activities of these herbs to prevent hyperlipidemia were identified and the new ingredients that emerged after fermentation warrant further exploration.
基金Fundamental Research Funds for the Central Universities of China(2662019PY066,2662016PY132)。
文摘This study investigated hypolipidemic,weight-reducing,and hepatoprotective effects of Antrodia cinnamomea mycelial extract obtained from solid-state culture(ACME)in an HFr D-induced obese/hyperlipidemic mouse model.Following 4-week ACME treatment,body weight,epididymal fat index,and some serum biochemical indices were measured.Expression levels of some related genes involved in cholesterol and lipid metabolism were analyzed by q RT-PCR.Moreover,histological studies of hepatic tissues were also conducted.After ACME treatment,body weight and epididymal fat index were significantly lower than that in model control group.ACME and simvastatin significantly reduced serum total cholesterol(T-CHO)and low-density lipoprotein cholesterol(LDL-C)levels,and increased high-density lipoprotein cholesterol(HDL-C)level.Subsequent experiments showed that:(i)ACME regulated transcriptional expression of 3-hydroxy-3-methylglutaryl-Co A reductase(HMGR),low-density lipoprotein receptor(LDLR),adipose triglyceride lipase(ATGL),and fatty acid synthase(FAS),with consequent reduction of blood lipid levels and body weight;(ii)ACME enhanced total antioxidant capacity(T-AOC)and superoxide dismutase(SOD)activity in hepatic tissues;(iii)ACME reduced malondialdehyde(MDA)level and ameliorated lipid oxidative damage in liver.Our findings indicate that ACME is a strong candidate for development as a novel anti-hyperlipidemia and anti-obesity health product.
基金supported by Shandong Provincial Natural Science Foundation,China(ZR2020MH373)National Natural Science Foundation of China(81503252)+2 种基金the support from High Level Traditional Chinese Medicine Key Disciplines of the State Administration of Traditional Chinese MedicinePharmacy Platform of Experimental Center of Shandong University of Traditional Chinese Medicinethe support of the Young Scientific Research Innovation Team of Shandong University of Traditional Chinese Medicine。
文摘The objective of this study was to establish an efficient and low-cost method for preparing glucoraphenin(GRE)from stir-fried seeds of Raphanus sativus L.,and then evaluate the anti-hyperlipidemia activity of GRE.The optimal resin was selected by comparing adsorption and desorption ability of six kinds of anion exchange resins for GRE.The loading and elution conditions of the optimal resin for GRE were then optimized by dynamic adsorption and desorption experiments.The results showed that D301-Cl^(-)resin was the optimal resin,and the optimal loading conditions were the original sample solution(pH=6)with a concentration of 0.10 g/mL,the sample loading flow rate was 2 BV/h,and the sample volume was 7 BV The optimum elution conditions were as follows:the elution solution was 5 g/100 mL NaCl,followed by subsequent elution with 4 BV at an elution flow rate of 2 BV/h.The collected eluent was further purified by ultrafiltration and preparative high-performance liquid chromatography(pHPLC)to obtain GRE,and the molecular weight and structure of GRE were determined by high performance liquid chromatography time-of-flight mass spectrometry and nuclear magnetic resonance,respectively.The purity of GRE was detected to be 95.1%by HPLC.Animal experiments showed that GRE had anti-hyperlipidemic effects by improving serum and liver lipid levels,alleviating liver lipid deposition,and protecting the liver.This study indicated that GRE may be a potential anti-hyperlipidemia drug candidate or dietary supplement.
文摘对长白山红松籽油调节血脂作用进行研究。建立昆明小白鼠高脂血症模型后,观察红松籽油调节血脂功能。通过测定血清总胆固醇(total cholesterol,TC)、甘油三酯(triglycerides,TG)、高密度脂蛋白胆固醇(high density lipoprotein cholesterol,HDL-C)、低密度脂蛋白胆固醇(low density lipoprotein cholesterol,LDL-C)、葡萄糖(glucose,Glu)含量以及肝脏和肾脏的TC、TG水平来检测长白山红松籽油的降血脂效应。结果表明:不同剂量的红松籽油均不同程度地降低高脂饲料对受试小白鼠高血脂症的诱导作用,显著改善肝脏及肾脏脂质的积累,红松籽油具有显著的降血脂效应。
