近年来,人们在对肠道微生物进行研究的过程中,发现了一种与肠道黏液层息息相关的微生物,名为阿克曼菌(Akkermansia muciniphila)。阿克曼菌是十多年前首次被分离出来的、定植于肠黏膜黏液层的一种细菌,它是疣微菌门、疣微菌纲、疣微菌...近年来,人们在对肠道微生物进行研究的过程中,发现了一种与肠道黏液层息息相关的微生物,名为阿克曼菌(Akkermansia muciniphila)。阿克曼菌是十多年前首次被分离出来的、定植于肠黏膜黏液层的一种细菌,它是疣微菌门、疣微菌纲、疣微菌目、疣微菌科被培养出来的第一株细菌[1],占健康成人粪便中细菌总数的1%~3%[2],具有分解黏蛋白的特征,可以以黏蛋白作为唯一的碳源和氮源进行生长。越来越多的研究发现阿克曼菌与肥胖、炎症性肠病(IBD)等疾病之间关系密切,多项动物及人体试验均证实体重和炎性反应标志物等水平与阿克曼菌的丰度呈负相关,且这种作用在细菌被巴氏杀菌后依然存在[3]。Patrice D Cani团队在阐明阿克曼菌的作用机制过程中首次发现,阿克曼菌的菌毛样外膜蛋白Amuc_1100能够独立发挥部分减脂和维护肠道屏障功能的作用。现就阿克曼菌外膜蛋白Amuc_1100研究进展综述如下。展开更多
Recent researches have reported that pasteurized Akkermansia muciniphila,as a paraprobiotic,still plays an active role in various metabolic diseases.However,its role in alcoholic liver disease(ALD)remains unexplored.T...Recent researches have reported that pasteurized Akkermansia muciniphila,as a paraprobiotic,still plays an active role in various metabolic diseases.However,its role in alcoholic liver disease(ALD)remains unexplored.This study aims to evaluate the impact and mechanisms of pasteurized A.muciniphila on ALD.Its effects on ALD were assessed by phenotypic,biochemical parameters,and histological features in a mouse model.Further investigations into underlying mechanisms were conducted through gut microbiome,metabolomics analyses and quantitative real-time polymerase chain reaction(qRT-PCR)technology.Heterologously expressed outer membrane protein Amuc_1100 was utilized to explore the molecular action mechanism.Our research demonstrated that pasteurized A.muciniphila can indeed alleviate alcohol-induced liver damage by modulation of gut microbiota and host metabolism.It suppressed the proliferation of pathogenic microbes and fostered the growth of probiotics.Additionally,it rectified alcohol-induced abnormalities in serum metabolic patterns,particularly in lipid metabolism.Specifically,pasteurized A.muciniphila elevated serum levels of 3-hydroxybutyric acid,palmitoleic acid,acetylcarnitine,DHA,and arachidonic acid in alcohol-fed mice.qRT-PCR results showed that it reversed the alcohol-induced upregulation of mRNA expression of hepatic fatty acid synthesis genes and decreased mRNA expression of fatty acid oxidation and transport genes.Importantly,we identified that Amuc_1100 could partially recapitulate the protective effects of pasteurized A.muciniphila on ALD,especially regarding hepatic lipid metabolism.This study enhances our comprehension of the function of pasteurized A.muciniphila and underscores its potential therapeutic application,along with Amuc_1100,in the treatment of ALD.展开更多
文摘近年来,人们在对肠道微生物进行研究的过程中,发现了一种与肠道黏液层息息相关的微生物,名为阿克曼菌(Akkermansia muciniphila)。阿克曼菌是十多年前首次被分离出来的、定植于肠黏膜黏液层的一种细菌,它是疣微菌门、疣微菌纲、疣微菌目、疣微菌科被培养出来的第一株细菌[1],占健康成人粪便中细菌总数的1%~3%[2],具有分解黏蛋白的特征,可以以黏蛋白作为唯一的碳源和氮源进行生长。越来越多的研究发现阿克曼菌与肥胖、炎症性肠病(IBD)等疾病之间关系密切,多项动物及人体试验均证实体重和炎性反应标志物等水平与阿克曼菌的丰度呈负相关,且这种作用在细菌被巴氏杀菌后依然存在[3]。Patrice D Cani团队在阐明阿克曼菌的作用机制过程中首次发现,阿克曼菌的菌毛样外膜蛋白Amuc_1100能够独立发挥部分减脂和维护肠道屏障功能的作用。现就阿克曼菌外膜蛋白Amuc_1100研究进展综述如下。
基金supported by the National Natural Science Foundation of China(Grant No.22108095)he Fundamental Research Funds for the Central Universities,Jiangnan University(Grant No.JUSRP123041)Figdraw(www.figdraw.com)the for its technical support(ID:PROYW47448).
文摘Recent researches have reported that pasteurized Akkermansia muciniphila,as a paraprobiotic,still plays an active role in various metabolic diseases.However,its role in alcoholic liver disease(ALD)remains unexplored.This study aims to evaluate the impact and mechanisms of pasteurized A.muciniphila on ALD.Its effects on ALD were assessed by phenotypic,biochemical parameters,and histological features in a mouse model.Further investigations into underlying mechanisms were conducted through gut microbiome,metabolomics analyses and quantitative real-time polymerase chain reaction(qRT-PCR)technology.Heterologously expressed outer membrane protein Amuc_1100 was utilized to explore the molecular action mechanism.Our research demonstrated that pasteurized A.muciniphila can indeed alleviate alcohol-induced liver damage by modulation of gut microbiota and host metabolism.It suppressed the proliferation of pathogenic microbes and fostered the growth of probiotics.Additionally,it rectified alcohol-induced abnormalities in serum metabolic patterns,particularly in lipid metabolism.Specifically,pasteurized A.muciniphila elevated serum levels of 3-hydroxybutyric acid,palmitoleic acid,acetylcarnitine,DHA,and arachidonic acid in alcohol-fed mice.qRT-PCR results showed that it reversed the alcohol-induced upregulation of mRNA expression of hepatic fatty acid synthesis genes and decreased mRNA expression of fatty acid oxidation and transport genes.Importantly,we identified that Amuc_1100 could partially recapitulate the protective effects of pasteurized A.muciniphila on ALD,especially regarding hepatic lipid metabolism.This study enhances our comprehension of the function of pasteurized A.muciniphila and underscores its potential therapeutic application,along with Amuc_1100,in the treatment of ALD.
