This work is an attempt to make a taxonomic study on Calotropis procera plant in Tehama area, Al-Baha region of Saudi Arabia. Al-Baha Province is divided by huge and steep Rocky Mountains into two main sectors, a lowl...This work is an attempt to make a taxonomic study on Calotropis procera plant in Tehama area, Al-Baha region of Saudi Arabia. Al-Baha Province is divided by huge and steep Rocky Mountains into two main sectors, a lowland coastal plain at the west, known as “Tihama” and a mountainous area with an elevation of (1500) to (2450) m above sea level at the east, known as “Al-Sarat or Al-Sarah” which form a part of Al-Sarawat Mountains range. The study included a scientific description of the family Apocynaceae and the scientific classification of the species Calotropis procera. Botanical names, synonyms and vernacular names (Local names) were presented. Botanical description is determined depending on its vegetative, floral and fruit characteristics. Notes on habitats and distribution were provided. The plant species has been illustrated by photos in its habitat. Chemical components, medicinal properties and local uses were included. The study revealed that Calotropis procera is widely spread throughout the study area and it has great medicinal importance, so it requires more comprehensive studies for identification. The study aims to: (Number one is deleted). 1) Determine the distribution and habits of the species Calotropis procera. 2) Provide medicinal properties and local uses of Calotropis procera. The method was recorded due to Dr. Jacob Thomas, Herbarium Dept., College of Science, King Saud University. Authors recommend recent accurate techniques such as DNA fingerprinting to identify the species. More Biochemical studies on the effective chemical compounds are required for the medicinal importance of the plant.展开更多
BACKGROUND Kushenol I(KSCI)exhibits potential anti-inflammatory and antioxidant activities.However,its therapeutic effects and mechanisms in ulcerative colitis(UC)remain unclear.AIM To investigate the therapeutic effe...BACKGROUND Kushenol I(KSCI)exhibits potential anti-inflammatory and antioxidant activities.However,its therapeutic effects and mechanisms in ulcerative colitis(UC)remain unclear.AIM To investigate the therapeutic effects and mechanisms of KSCI in alleviating UC.METHODS Therapeutic targets for KSCI in treating UC were identified using network pharmacology.Molecular docking and dynamics simulations confirmed the interactions between KSCI and these targets.In a murine UC model induced by dextran sodium sulfate(DSS),the anti-inflammatory and antioxidant effects of KSCI were evaluated following oral administration,as well as its impact on intestinal barrier function and immune response modulation.Finally,changes in gut microbiota composition were analyzed using 16S ribosomal RNA sequencing.RESULTS A total of 192 potential targets of KSCI in treating UC were identified using network pharmacology.KSCI bound stably to core targets including protein kinase B(AKT),p38 mitogen-activated protein kinase(p38 MAPK),NOD-like receptor thermal protein domain associated protein 3(NLRP3),phosphoinositide 3-kinase(PI3K),forkhead box O1(FOXO1),and Toll-like receptor 4(TLR4).The oral administration of KSCI improved colon length and body weight,and reduced disease activity in a mouse model of DSS-induced UC.KSCI suppressed pro-inflammatory cytokines(interleukin[IL-1β],IL-6,IL-17,and tumor necrosis factor alpha)and promoted the expression of the anti-inflammatory cytokine IL-10.It also inhibited key signaling molecules and modulated the expression of IL-1β,AKT,p38 MAPK,NLRP3,PI3K,AKT,FOXO1,and TLR4.KSCI exhibited potent antioxidant effects,ameliorating colonic inflammation and tissue damage.It improved intestinal barrier function,influenced gut microbiota composition,and increased splenic T-cell percentages.CONCLUSION KSCI alleviated DSS-induced UC by modulating gut microbiota,enhancing the intestinal barrier,reducing inflam-mation and oxidative stress,and regulating the immune response.展开更多
文摘This work is an attempt to make a taxonomic study on Calotropis procera plant in Tehama area, Al-Baha region of Saudi Arabia. Al-Baha Province is divided by huge and steep Rocky Mountains into two main sectors, a lowland coastal plain at the west, known as “Tihama” and a mountainous area with an elevation of (1500) to (2450) m above sea level at the east, known as “Al-Sarat or Al-Sarah” which form a part of Al-Sarawat Mountains range. The study included a scientific description of the family Apocynaceae and the scientific classification of the species Calotropis procera. Botanical names, synonyms and vernacular names (Local names) were presented. Botanical description is determined depending on its vegetative, floral and fruit characteristics. Notes on habitats and distribution were provided. The plant species has been illustrated by photos in its habitat. Chemical components, medicinal properties and local uses were included. The study revealed that Calotropis procera is widely spread throughout the study area and it has great medicinal importance, so it requires more comprehensive studies for identification. The study aims to: (Number one is deleted). 1) Determine the distribution and habits of the species Calotropis procera. 2) Provide medicinal properties and local uses of Calotropis procera. The method was recorded due to Dr. Jacob Thomas, Herbarium Dept., College of Science, King Saud University. Authors recommend recent accurate techniques such as DNA fingerprinting to identify the species. More Biochemical studies on the effective chemical compounds are required for the medicinal importance of the plant.
基金Supported by the National Natural Science Foundation of China,No.82060707 and No.82104381Application and Basis Research Project of Yunnan China,No.202201AW070016+2 种基金Central Special Fund for Guiding Local Science and Technology Development,No.202407AB110018Engineering Research Center of Yunnan Education Department,No.2022YGG03Open Research Fund Program of Yunnan Key Laboratory of Integrated Traditional Chinese and Western Medicine for Chronic Disease in Prevention and Treatment,No.2019DG016.
文摘BACKGROUND Kushenol I(KSCI)exhibits potential anti-inflammatory and antioxidant activities.However,its therapeutic effects and mechanisms in ulcerative colitis(UC)remain unclear.AIM To investigate the therapeutic effects and mechanisms of KSCI in alleviating UC.METHODS Therapeutic targets for KSCI in treating UC were identified using network pharmacology.Molecular docking and dynamics simulations confirmed the interactions between KSCI and these targets.In a murine UC model induced by dextran sodium sulfate(DSS),the anti-inflammatory and antioxidant effects of KSCI were evaluated following oral administration,as well as its impact on intestinal barrier function and immune response modulation.Finally,changes in gut microbiota composition were analyzed using 16S ribosomal RNA sequencing.RESULTS A total of 192 potential targets of KSCI in treating UC were identified using network pharmacology.KSCI bound stably to core targets including protein kinase B(AKT),p38 mitogen-activated protein kinase(p38 MAPK),NOD-like receptor thermal protein domain associated protein 3(NLRP3),phosphoinositide 3-kinase(PI3K),forkhead box O1(FOXO1),and Toll-like receptor 4(TLR4).The oral administration of KSCI improved colon length and body weight,and reduced disease activity in a mouse model of DSS-induced UC.KSCI suppressed pro-inflammatory cytokines(interleukin[IL-1β],IL-6,IL-17,and tumor necrosis factor alpha)and promoted the expression of the anti-inflammatory cytokine IL-10.It also inhibited key signaling molecules and modulated the expression of IL-1β,AKT,p38 MAPK,NLRP3,PI3K,AKT,FOXO1,and TLR4.KSCI exhibited potent antioxidant effects,ameliorating colonic inflammation and tissue damage.It improved intestinal barrier function,influenced gut microbiota composition,and increased splenic T-cell percentages.CONCLUSION KSCI alleviated DSS-induced UC by modulating gut microbiota,enhancing the intestinal barrier,reducing inflam-mation and oxidative stress,and regulating the immune response.
