Phytochromes in seed plants are known to move into nuclei in a red light-dependent manner with or without interacting factors. Here, we show phytochrome relocation to the nuclear region in phytochrome-dependent Adiant...Phytochromes in seed plants are known to move into nuclei in a red light-dependent manner with or without interacting factors. Here, we show phytochrome relocation to the nuclear region in phytochrome-dependent Adiantum capillus-veneris spore germination by partial spore-irradiation experiments. The nuclear or non-nuclear region of imbibed spores was irradiated with a microbeam of red and/or far-red light and the localization of phytochrome involved in spore germination was estimated from the germination rate. The phytochrome for spore germination existed throughout whole spore under darkness after imbibition, but gradually migrated to the nuclear region following red light irradiation. In- tracellular distribution of PHY-GUS fusion proteins expressed in germinated spores by particle bombardment showed the migration of Acphy2, but not Acphyl, into nucleus in a red light-dependent manner, suggesting that Acphy2 is the photoreceptor for fern spore germination.展开更多
利用光学显微镜和扫描电子显微镜对产于广西的3种铁线蕨属植物的孢子形态进行了观察研究,详细描述了3种铁线蕨属植物即铁线蕨A d iantum cap illus-veneris L.、扇叶铁线蕨A.f labellu la tum L.和假鞭叶铁线蕨A.m a lesianum G hatak...利用光学显微镜和扫描电子显微镜对产于广西的3种铁线蕨属植物的孢子形态进行了观察研究,详细描述了3种铁线蕨属植物即铁线蕨A d iantum cap illus-veneris L.、扇叶铁线蕨A.f labellu la tum L.和假鞭叶铁线蕨A.m a lesianum G hatak孢子的形态及表面纹饰特征,讨论了各种间的差异。展开更多
文摘Phytochromes in seed plants are known to move into nuclei in a red light-dependent manner with or without interacting factors. Here, we show phytochrome relocation to the nuclear region in phytochrome-dependent Adiantum capillus-veneris spore germination by partial spore-irradiation experiments. The nuclear or non-nuclear region of imbibed spores was irradiated with a microbeam of red and/or far-red light and the localization of phytochrome involved in spore germination was estimated from the germination rate. The phytochrome for spore germination existed throughout whole spore under darkness after imbibition, but gradually migrated to the nuclear region following red light irradiation. In- tracellular distribution of PHY-GUS fusion proteins expressed in germinated spores by particle bombardment showed the migration of Acphy2, but not Acphyl, into nucleus in a red light-dependent manner, suggesting that Acphy2 is the photoreceptor for fern spore germination.
文摘利用光学显微镜和扫描电子显微镜对产于广西的3种铁线蕨属植物的孢子形态进行了观察研究,详细描述了3种铁线蕨属植物即铁线蕨A d iantum cap illus-veneris L.、扇叶铁线蕨A.f labellu la tum L.和假鞭叶铁线蕨A.m a lesianum G hatak孢子的形态及表面纹饰特征,讨论了各种间的差异。
