Extensive use of avermectin(AVM)in agriculture can seriously contaminate fish in aquaculture.Ferulic acid(FA)is a strong antioxidant sourced from plants and is widely applied in food and medicine,but its protective fu...Extensive use of avermectin(AVM)in agriculture can seriously contaminate fish in aquaculture.Ferulic acid(FA)is a strong antioxidant sourced from plants and is widely applied in food and medicine,but its protective function on the immunological damage caused by AVM is still lacking.In this study,carp were chronically exposed to(1/1096 hr LC_(50))AVM for 30 day(s),with a dietary supplement of FA(400 mg/kg)to assess its effects on carp spleen.The experimental groups(n=10)included:control,AVM-exposed(2.404μg/L),FA+AVM co-treated,and FA alone.Long-term AVM exposure altered spleen tissue,reducing serum complement C3(p<0.01)and immunoglobulin M levels(p<0.001),and increasing proinflammatory tnf-α(p<0.001),il-6(p<0.001),il-1β(p<0.001),and inos mRNA levels,whilst down-regulating the anti-inflammatory tgf-β(p<0.05).Additionally,it disrupted the balance of oxidative stress indicators such asMDA(p<0.001),T-AOC,GSH,and CAT,leading to spleen tissue apoptosis(42.4%).However,the addition of FA reversed these conditions,elevated the anti-inflammatory factor,and improved spleen immune function following chronic exposure to AVMin carp.Moreover,the ability to restore oxidative homeostasis in carp by adjusting the Nrf2/NQO-1 axis protected the health of spleen tissues.This discovery also supports the development of fish feed for aquaculture.展开更多
本文用动态法测定averm ectin在甲醇、乙醇、正丙醇、正丁醇和正戊醇中的溶解度曲线,并分别用理想溶液模型、A pe l-b lat溶解度模型和多项式经验方程对实验测定溶解度数据进行关联。结果显示理想溶液模型的误差较大,多项式经验方程的...本文用动态法测定averm ectin在甲醇、乙醇、正丙醇、正丁醇和正戊醇中的溶解度曲线,并分别用理想溶液模型、A pe l-b lat溶解度模型和多项式经验方程对实验测定溶解度数据进行关联。结果显示理想溶液模型的误差较大,多项式经验方程的误差最小。实验得到的溶解度曲线及关联结果对averm ectin结晶工艺的研究具有较大的指导意义。展开更多
[Objective] Strain Biok Av-023 used as the control was employed on screening of high-avermectin yield mutants by rational screening.[Method] With Biok Av-023 as the original strain,the positive mutation strain was fir...[Objective] Strain Biok Av-023 used as the control was employed on screening of high-avermectin yield mutants by rational screening.[Method] With Biok Av-023 as the original strain,the positive mutation strain was firstly screened by routine UV mutagenesis,and then the high-yield avermectin producing strain was selected by the breeding way inferred by L-Ile induction.[Result] UV mutation and L-Ile directional screening had showed that the best L-Ile screening concentration was 0.5%,and the high-yield mutation strain AV60s-32 after re-screening reached the highest titer of 4520 IU/ml,which increased by 23.4% compared with the original strain.[Conclusion] The production of avermectin can be effectively enhanced by the combined way of UV mutation and L-Ile rational breeding.展开更多
基金supported by the National Natural Science Foundation of China(No.81773968)the Priority Academic Program Development of Jiangsu Higher Education Institutions of China.
文摘Extensive use of avermectin(AVM)in agriculture can seriously contaminate fish in aquaculture.Ferulic acid(FA)is a strong antioxidant sourced from plants and is widely applied in food and medicine,but its protective function on the immunological damage caused by AVM is still lacking.In this study,carp were chronically exposed to(1/1096 hr LC_(50))AVM for 30 day(s),with a dietary supplement of FA(400 mg/kg)to assess its effects on carp spleen.The experimental groups(n=10)included:control,AVM-exposed(2.404μg/L),FA+AVM co-treated,and FA alone.Long-term AVM exposure altered spleen tissue,reducing serum complement C3(p<0.01)and immunoglobulin M levels(p<0.001),and increasing proinflammatory tnf-α(p<0.001),il-6(p<0.001),il-1β(p<0.001),and inos mRNA levels,whilst down-regulating the anti-inflammatory tgf-β(p<0.05).Additionally,it disrupted the balance of oxidative stress indicators such asMDA(p<0.001),T-AOC,GSH,and CAT,leading to spleen tissue apoptosis(42.4%).However,the addition of FA reversed these conditions,elevated the anti-inflammatory factor,and improved spleen immune function following chronic exposure to AVMin carp.Moreover,the ability to restore oxidative homeostasis in carp by adjusting the Nrf2/NQO-1 axis protected the health of spleen tissues.This discovery also supports the development of fish feed for aquaculture.
基金Supported by 863 Project (2009AA032904 )Scientific ResearchStarting-up Project for Young Teachers in Changshu Institute oTechnology" Hundreds of Entrepreneurs into Campus" Project inChangshu Institute of Technology~~
文摘[Objective] Strain Biok Av-023 used as the control was employed on screening of high-avermectin yield mutants by rational screening.[Method] With Biok Av-023 as the original strain,the positive mutation strain was firstly screened by routine UV mutagenesis,and then the high-yield avermectin producing strain was selected by the breeding way inferred by L-Ile induction.[Result] UV mutation and L-Ile directional screening had showed that the best L-Ile screening concentration was 0.5%,and the high-yield mutation strain AV60s-32 after re-screening reached the highest titer of 4520 IU/ml,which increased by 23.4% compared with the original strain.[Conclusion] The production of avermectin can be effectively enhanced by the combined way of UV mutation and L-Ile rational breeding.
