The ecdysone-induced transcription factor E93 in model insects plays multiple roles in the insect metamorphosis processes,such as remodeling larval tissues and determining adult tissue formation.The knockdown of E93in...The ecdysone-induced transcription factor E93 in model insects plays multiple roles in the insect metamorphosis processes,such as remodeling larval tissues and determining adult tissue formation.The knockdown of E93in insects leads to incomplete metamorphosis,suggesting that E93 is a potential target for pest control.In this study,the HaE93 gene in the cotton bollworm Helicoverpa armigera,a polyphagous pest of various commercial crops worldwide,was identified and found to have high expression in the egg,prepupal,and pupal stages.The injection of ds HaE93 induced about 60%mortality in H.armigera at the larval-pupal stage.About 30%survived but showed delayed pupation and abnormal wings,and the females developed reduced ovaries.Therefore,about 90%of the HaE93 knockdown individuals failed to reproduce before they died.The results of qRT-PCR showed that the expression levels of ecdysone primary-response genes,chitin synthesis-related genes,and wing and ovary development-related genes were reduced in HaE93 knockdown H.armigera.These results indicated that HaE93plays a critical role in larva-pupa-adult metamorphosis and the development of the cuticle,wing,and ovary in female H.armigera by regulating the expression of the associated genes.Bioassays of ds HaE93 administered by either oral delivery or injection showed similar knockdown results,which suggested that HaE93 can be used as a target gene for the RNAi control of the pest H.armigera.展开更多
Mating behavior is essential for sexual reproduction,and it is often modulated by key chemical cues.In many moth species,males find compatible mates through the reception of sex pheromones which are released by female...Mating behavior is essential for sexual reproduction,and it is often modulated by key chemical cues.In many moth species,males find compatible mates through the reception of sex pheromones which are released by females.Pheromone receptors(PRs)are key elements in sensing these chemical signals.Concurrently,male moths emit a complex blend of volatile compounds during courtship;however,the mechanisms for recognizing putative male pheromones remain poorly understood.Here,we employed gas chromatography coupled with electroantennographic detection and mass spectrometry to analyze the volatile compounds produced by males of the cotton bollworm,Helicoverpa armigera.Three candidate male sex pheromones were identified,with(Z)-7-dodecen-1-yl acetate(Z7-12:OAc)eliciting the most pronounced electrophysiological response in the male antenna.The olfactory receptor neuron(ORN)ORN-a in Type A trichoid sensilla was shown to respond to Z7-12:OAc by conducting single sensillum recording(SSR)assays.Additionally,we found that the OR13s from five Heliothinae species responded to Z7-12:OAc by using the Xenopus oocyte expression system and two-electrode voltage-clamp recording.Our findings identified a candidate for evaluation in future behavioral studies of the poorly understood chemosensory recognition mechanisms underlying male sex pheromones.If its relevance is supported by behavioral data,this knowledge may facilitate the design of novel olfactory regulators for effective pest control strategies involving mating disruption.展开更多
[Objective] Using molecular biotechnology to clone the proteasome β5 gene from cotton bollworm (Helicoverpa armigera), this research aimed to provide basis for further research on the function of proteasome β5 gene ...[Objective] Using molecular biotechnology to clone the proteasome β5 gene from cotton bollworm (Helicoverpa armigera), this research aimed to provide basis for further research on the function of proteasome β5 gene in cotton bollworm. [Method] Total RNA was extracted from midgut of cotton bollworm. The full length cDNA of Habeta5 gene was cloned by using rapid amplification of cDNA ends (RACE) technology, then sequence analysis was carried out. [Result] The full length cDNA sequence was successfully cloned and isolated, named as Habeta5. It was 947 bp in length, contained an ORF (843 bp) and encoded 280 amino acid residues, with the predicted mass of 30.87 kD and isoelectric point(pI) of 9.60. In the deduced amino acid sequence, a proteasome β5 subunit domain lies between 74th to 261st amino acid residues. It has more than 62% identity to other insects such as Drosophila melanogaster. The proteasome β5 subunit conservative regions were very similar with each other. Molecular evolution by Neighbor Joining method indicated that Habeta5 was homologous with other proteasome β5 subunit of species. [Conclusion] Sequence alignment shows that the cloned fragment is a proteasome β5 subunit gene (GenBank accession number: FJ358434).展开更多
[ Objective ] The paper was to confirm the effect of mating and black light treatment on cryptochromes mRNA expression of Hclicoverpa armigera. [ Method ] Quantitative real-time PCR （ SYBR Green） technique was appli...[ Objective ] The paper was to confirm the effect of mating and black light treatment on cryptochromes mRNA expression of Hclicoverpa armigera. [ Method ] Quantitative real-time PCR （ SYBR Green） technique was applied to detect the expression of cryptochromes gene （ cryl and cry2 ） of H. armigera under different conditions. Total RNA was extracted from the head of H. armigera, and carried out reverse transcription to synthesize cDNA after digested by DNase I. Specific primers were used to carry out quantitative real-time PCR on cryl, cry2 and EF-Ict gene, respectively. [ Result] The expression of cryl mRNA of H. ar- migera significantly decreased after exposure to black light for 2 h, the mRNA expression of cry2 was smaller than control, they had no significant difference with each other. Mating had significant effect on mRNA expression of cryl and cry2 of H. armigera, and the mRNA expression of cryl and cry2 of male and female adults showed decreasing trend with the prolongation of time after mating. [ Conclusion] The result had important significance for further studying on function of cry gene and the control of cotton bollwonn.展开更多
[Objective] We aimed to investigate the relationship between temperature and occurrence of Helicoverpa armigera （H0bner） in Shihezi Zong Chang. [Method] The data of occurrence of adult Helicoverpa armigera （Hubner...[Objective] We aimed to investigate the relationship between temperature and occurrence of Helicoverpa armigera （H0bner） in Shihezi Zong Chang. [Method] The data of occurrence of adult Helicoverpa armigera （Hubner） and temperature in 2007-2010 was analyzed for building the prediction model. [Result] The amount of overwintering generation of Helicoverpa armigera （Hubner） increased with the in- crease of temperature and its population dynamics showed a positive correlation with temperature. The amount of first and second generation of Helicoverpa armigera （Hubner） decreased with the increase of temperature and its population dynamics showed a negative correlation with temperature. [Conclusion] This research would provide a basis for the forecast and prevention of Helicoverpa armigera （Hubner） in North Xinjiang.展开更多
Sublethal effects of chlorantraniliprole to Helicoverpa armigera （Hiibner） larvae were evaluated through exposure of third instar larvae to the insecticide incorporated into an artificial diet. When larvae were fed ...Sublethal effects of chlorantraniliprole to Helicoverpa armigera （Hiibner） larvae were evaluated through exposure of third instar larvae to the insecticide incorporated into an artificial diet. When larvae were fed on the insecticide-containing diet for 7 d, the LC10, LC20, LC40, and LC50values were 3.790, 7.977, 21.577, and 33.121 μg active ingredient L^-1, respectively. Chlorantraniliprole at sublethal concentrations significantly reduced the larval body mass, emergence ratio, adult longevity and egg hatching rate in both the parental and offspring generations. The pupation and copulation rate in the parental generation and the pupal mass in the offspring also strongly decreased. Reproduction was seriously disturbed in both the parental and offspring groups even when only one of the partners was exposed to chlorantraniliprole at larval stages. However, at the lowest concentration of exposure （LC^0）, offspring fecundity was strongly reduced only when both partners were exposed. The net reproductive rate （R0）, intrinsic rate of increase （rm）, and gross reproduction rate （GRR） at LC20, LC40 concentrations were significantly lower than that of the control. Post-exposure effects also included an extended larval developmental time and increased male proportion in both generations. The doubling time （Dt） at LC20, LC40 concentrations as well as gross reproduction rate （GRR） at LC10 concentration were also significantly increased. Chlorantraniliprole might have significant effects on H. armigera population dynamics even at sublethal concentrations on both parental and offspring generations.展开更多
The full-length sequence of the odorant binding protein 5 gene,HarmOBP5,was obtained from an antennae cDNA library of cotton bollworm,Helicoverpa armigera （Hübner）.The cDNA contains a 444 bp open reading frame,...The full-length sequence of the odorant binding protein 5 gene,HarmOBP5,was obtained from an antennae cDNA library of cotton bollworm,Helicoverpa armigera （Hübner）.The cDNA contains a 444 bp open reading frame,encoding a protein with 147 amino acids,namely HarmOBP5.HarmOBP5 was expressed in Escherichia coli and the recombinant protein was purified by affinity chromatography.SDS-PAGE and Western blot analysis demonstrated that the purified protein can be used for further investigation of its binding characteristics.Competitive binding assays with 113 odorant chemicals indicated that HarmOBP5 has strong affinity to some special plant volatiles,including （E）-β-farnesene,ethyl butyrate,ethyl heptanoate,and acetic acid 2-methylbutyl ester.Based on three-dimensional （3D） model of AaegOBP1 from Aedes aegypti,a 3D model of HarmOBP5 was predicted.The model revealed that some key binding residues in HarmOBP5 may play important roles in odorant perception of H.armigera.This study provides clues for better understanding physiological functions of OBPs in H.armigera and other insects.展开更多
The growth, development and consumption of successive three generations of cotton bollworm, Helicoverpa armigera （Htibner）, fed on cotton bolls grown under elevated CO2 （double-ambient vs. ambient） in open-top cha...The growth, development and consumption of successive three generations of cotton bollworm, Helicoverpa armigera （Htibner）, fed on cotton bolls grown under elevated CO2 （double-ambient vs. ambient） in open-top chambers were examined. Significant decreases in protein, total amino acid, water and nitrogen content and increases in free fatty acid were observed in cotton bolls. Changes in quality of cotton bolls affected the growth, development and food utilization of H. armigera. Significantly longer larval development duration in three successive generations and lower pupal weight of the second and third generations were observed in cotton bollworm fed on cotton bolls grown under elevated CO2. Significantly lower fecundity was also found in successive three generations of H. armigera fed on cotton bolls grown under elevated CO2. The consumption per larva occurred significant increase in successive three generations and frass per larva were also significantly increased during the second and third generations under elevated CO2. Significantly lower relative growth rate, efficiency of conversion of ingested food and significant higher relative consumption rate in successive three generations were observed in cotton bollworm fed on cotton bolls grown under elevated CO2. Significantly lower potential female fecundity, larval numbers and population consumption were found in the second and third generations of cotton bollworm fed on cotton bolls grown under elevated CO2. The integrative effect of higher larval mortality rate and lower adult fecundity resulted in significant decreases in potential population consumption in the latter two generations. The results show that elevated CO2 adversely affects cotton bolls quality, which indicates the potential population dynamics and potential population consumption of cotton bollworm will alleviate the harm to the plants in the future rising CO2 atmosphere.展开更多
A chemosensory protein named HarmCSP5 in cotton bollworm Helicoverpa armigera （Hvbner） was obtained from antennal eDNA libraries and expressed in Escherichia coll. The real time quantitative PCR （RT-qPCR） results ...A chemosensory protein named HarmCSP5 in cotton bollworm Helicoverpa armigera （Hvbner） was obtained from antennal eDNA libraries and expressed in Escherichia coll. The real time quantitative PCR （RT-qPCR） results indicated that HarmCSP5 gene was mainly expressed in male and female antennae but also expressed in female legs and wings. Competitive binding assays were performed to test the binding affinity of recombinant HarmCSP5 to 60 odor molecules including some cotton volatiles. The resules showed that HarmCSP5 showed strong binding abilities to 4-ehtylbenzaldehyde and 3,4-dimethlbenz aldehyde, whereas methyl phenylacetate, 2-decanone, 1-pentanol, carvenol, isobomeol, nerolidol, 2- nonanone and ethyl heptanoate have relatively weak binding affinity. Moreover, the predicted 3D model of HarmCSP5 consists of six α-helices located among residues 33-38 （αl）, 40-48 （α2）, 62-72 （α3）, 80-96 （α4）, 98-108 （α5）, and 116-119 （α6）, two pairs of disulfide bridges Cys49-Cys55, Cys75-Cys78. The two amino acid residues, Ile94 and Trpl01, may play crucial roles in HarmCSP5 binding with ligands and need further study for confirmation.展开更多
Electroantennogram (EAG) evaluation of selected compounds from wilted leaves of black poplar, Populus nigra, showed that phenyl acetaldehyde, methyl salicylate, (E)-2-hexenal elicited strong responses from male antenn...Electroantennogram (EAG) evaluation of selected compounds from wilted leaves of black poplar, Populus nigra, showed that phenyl acetaldehyde, methyl salicylate, (E)-2-hexenal elicited strong responses from male antennae of Helicoverpa armigera. When mixed with sex pheromone (Ph), some volatiles, e.g. phenyl acetaldehyde, benzyl alcohol, phenylethanol, methylsalicylate, linalool, benzaldehyde, (Z)-3-hexenol, (Z)-3-hexenylacetate, (Z)-6-nonenol, cineole, (E)-2-hexenal, and geraniol elicited stronger responses from male antennae than Ph alone. Wind tunnel bioassay demonstrated that various volatiles could either enhance or inhibit the effect of synthetic sex pheromone. (E)-2-hexenal, (Z)-3-hexenol and linalool in combination with Ph could not induce any male to land on source at all, whereas phenyl acetaldehyde, benzaldehyde, (Z)-6-nonenol and salicylaldehyde combined with Ph enhanced male response rates by 58.63%, 50.33%, 51.85% and 127.78%, respectively, compared to Ph alone. These results suggested that some volatiles shouldmodify sex pheromone caused behavior and that some of them could possibly be used as a tool for disrupting mating or for enhancing the effect of synthetic sex pheromone in the field.展开更多
Light traps are widely utilized to monitor and manage insect pest populations.In late 2018,the fall armyworm(FAW),Spodoptera frugiperda,invaded China through Yunnan Province representing a huge threat to grain product...Light traps are widely utilized to monitor and manage insect pest populations.In late 2018,the fall armyworm(FAW),Spodoptera frugiperda,invaded China through Yunnan Province representing a huge threat to grain production.To estimate the efficiency of light traps on FAW moths,we first identified the opsin genes from FAW by using the transcriptome.Phylogenetic analysis indicated that the four opsins of FAW were clustered with those of other Noctuidae species.The expressed levels of opsins in S.frugiperda were lower than in Helicoverpa armigera,suggesting a different phototactic response between the two species.Then,we determined the phototactic behavior of FAW using H.armigera as a control,which is widely monitored and managed using light traps in China.Our results indicated that the two moths species showed significantly different phototactic behavior and both female and male FAW displayed faster flight-to-light speed than H.armigera.This may be due to a faster flight capacity in FAW compared to H.armigera.However,the capture rate of both female and male of S.frugiperda was significantly lower than that of H.armigera,which was consistent with the expression levels of opsins.These results support the positive phototaxis of S.frugiperda moths and suggest light traps could be used for monitoring and managing the pests,but with a lower efficiency than H.armigera.展开更多
Objective:To evaluate the antifeedant,insecticidal and growth inhibition activities of Solanum pseudocapsicum(S.pseudocapsicum)seed extracts against Spodoptera litura(S.litura)and Helicotverpa armigera(H.armigera).Met...Objective:To evaluate the antifeedant,insecticidal and growth inhibition activities of Solanum pseudocapsicum(S.pseudocapsicum)seed extracts against Spodoptera litura(S.litura)and Helicotverpa armigera(H.armigera).Methods:Hcxane,diethyl cther,dichloromethanc and ethyl acetate seed extracts were prepared and tested for autifeedant,insecticidal and growth inhibitory activities against fourth instar larvae of&litura and H.armigera.Results:Ethyl acetate extract showed promising antifeedant and insecticidal activities against S.litura and H.armigera.Percentage of deformed larvae,pupae and adults were maximum in treatment of ethyl acetate extract.Percentage of successful adult emergence was deteriorated by seeds on extract treated larvae.Conclusions:Ethyl acetate extracts of S.pseudocapsicum,showed higher efficiency of antifeedant,insecticidal and growth inhibition activities.Hence,it can be used to controll agricultural insect pests,S.litura and H.armigera.展开更多
The brush border membrane vesicles (BBMVs) in midgut of Helicoverpa armigera weresuccessfully separated, and most of the Aminopeptidase N (APN) activities in BBMV werepreserved. The 3-[(3-chlor-amidopropyl) dimethylam...The brush border membrane vesicles (BBMVs) in midgut of Helicoverpa armigera weresuccessfully separated, and most of the Aminopeptidase N (APN) activities in BBMV werepreserved. The 3-[(3-chlor-amidopropyl) dimethylammonio]-1-propane-sulphonate (CHAPS)can enhance the dissolution of BBMV, and phosphatidylinositol-specific phosopholipase C(PI-PLC) can cleave the APN from midgut membrane. The APN was primarily purified usinga Mono-Q column. The results of immunoblotting showed that the 120 and 170kDa proteinsin the BBMV could bind Cry1Ac, and 120kDa APN was a glycosylphosphalidylinositol(GPI)-anchored protein. Two Bt-resistant strains (Bt-P, Bt-M) were obtained after beingselected for more than five years in laboratory using Bt insecticides and Bt transgeniccotton incorporated into diet separately. The resistance of Bt-P and Bt-M were 1083.3and 48.7 times that of susceptible strain. The genes encoding APN1 in midgut ofsusceptible and resistant H.armigera were cloned by PCR and RACE techniques. Theinferred amino acid sequences of APN1 possessed the common character of APN family ininsects. In comparison with APN1 in susceptible strain, three nucleotide mutations wereobserved in the APN1 of Bt-M strain and resulted in two amino acid replace in theputative protein sequences, and eight nucleotide mutations were observed in Bt-P strainand resulted in five amino acid replace.展开更多
基金supported by the National Natural Science Foundation of China(32001912 and 32370525)the Henan Provincial Natural Science Foundation,China(232300420012)。
文摘The ecdysone-induced transcription factor E93 in model insects plays multiple roles in the insect metamorphosis processes,such as remodeling larval tissues and determining adult tissue formation.The knockdown of E93in insects leads to incomplete metamorphosis,suggesting that E93 is a potential target for pest control.In this study,the HaE93 gene in the cotton bollworm Helicoverpa armigera,a polyphagous pest of various commercial crops worldwide,was identified and found to have high expression in the egg,prepupal,and pupal stages.The injection of ds HaE93 induced about 60%mortality in H.armigera at the larval-pupal stage.About 30%survived but showed delayed pupation and abnormal wings,and the females developed reduced ovaries.Therefore,about 90%of the HaE93 knockdown individuals failed to reproduce before they died.The results of qRT-PCR showed that the expression levels of ecdysone primary-response genes,chitin synthesis-related genes,and wing and ovary development-related genes were reduced in HaE93 knockdown H.armigera.These results indicated that HaE93plays a critical role in larva-pupa-adult metamorphosis and the development of the cuticle,wing,and ovary in female H.armigera by regulating the expression of the associated genes.Bioassays of ds HaE93 administered by either oral delivery or injection showed similar knockdown results,which suggested that HaE93 can be used as a target gene for the RNAi control of the pest H.armigera.
基金funded by the National Natural Science Foundation of China(32272540 and 32072509)the National Key R&D Program of China(2022YFD1400800)the Innovation Program of Chinese Academy of Agricultural Sciences(CAAS-CSCB-202302)。
文摘Mating behavior is essential for sexual reproduction,and it is often modulated by key chemical cues.In many moth species,males find compatible mates through the reception of sex pheromones which are released by females.Pheromone receptors(PRs)are key elements in sensing these chemical signals.Concurrently,male moths emit a complex blend of volatile compounds during courtship;however,the mechanisms for recognizing putative male pheromones remain poorly understood.Here,we employed gas chromatography coupled with electroantennographic detection and mass spectrometry to analyze the volatile compounds produced by males of the cotton bollworm,Helicoverpa armigera.Three candidate male sex pheromones were identified,with(Z)-7-dodecen-1-yl acetate(Z7-12:OAc)eliciting the most pronounced electrophysiological response in the male antenna.The olfactory receptor neuron(ORN)ORN-a in Type A trichoid sensilla was shown to respond to Z7-12:OAc by conducting single sensillum recording(SSR)assays.Additionally,we found that the OR13s from five Heliothinae species responded to Z7-12:OAc by using the Xenopus oocyte expression system and two-electrode voltage-clamp recording.Our findings identified a candidate for evaluation in future behavioral studies of the poorly understood chemosensory recognition mechanisms underlying male sex pheromones.If its relevance is supported by behavioral data,this knowledge may facilitate the design of novel olfactory regulators for effective pest control strategies involving mating disruption.
基金Supported by National Basic Research Program of China(2005CB121005)~~
文摘[Objective] Using molecular biotechnology to clone the proteasome β5 gene from cotton bollworm (Helicoverpa armigera), this research aimed to provide basis for further research on the function of proteasome β5 gene in cotton bollworm. [Method] Total RNA was extracted from midgut of cotton bollworm. The full length cDNA of Habeta5 gene was cloned by using rapid amplification of cDNA ends (RACE) technology, then sequence analysis was carried out. [Result] The full length cDNA sequence was successfully cloned and isolated, named as Habeta5. It was 947 bp in length, contained an ORF (843 bp) and encoded 280 amino acid residues, with the predicted mass of 30.87 kD and isoelectric point(pI) of 9.60. In the deduced amino acid sequence, a proteasome β5 subunit domain lies between 74th to 261st amino acid residues. It has more than 62% identity to other insects such as Drosophila melanogaster. The proteasome β5 subunit conservative regions were very similar with each other. Molecular evolution by Neighbor Joining method indicated that Habeta5 was homologous with other proteasome β5 subunit of species. [Conclusion] Sequence alignment shows that the cloned fragment is a proteasome β5 subunit gene (GenBank accession number: FJ358434).
基金Supported by National"973"Project(No.2006CB102006)Special Research Project in Public Service Sectors(Agriculture)(201103012)~~
文摘[ Objective ] The paper was to confirm the effect of mating and black light treatment on cryptochromes mRNA expression of Hclicoverpa armigera. [ Method ] Quantitative real-time PCR （ SYBR Green） technique was applied to detect the expression of cryptochromes gene （ cryl and cry2 ） of H. armigera under different conditions. Total RNA was extracted from the head of H. armigera, and carried out reverse transcription to synthesize cDNA after digested by DNase I. Specific primers were used to carry out quantitative real-time PCR on cryl, cry2 and EF-Ict gene, respectively. [ Result] The expression of cryl mRNA of H. ar- migera significantly decreased after exposure to black light for 2 h, the mRNA expression of cry2 was smaller than control, they had no significant difference with each other. Mating had significant effect on mRNA expression of cryl and cry2 of H. armigera, and the mRNA expression of cryl and cry2 of male and female adults showed decreasing trend with the prolongation of time after mating. [ Conclusion] The result had important significance for further studying on function of cry gene and the control of cotton bollwonn.
文摘[Objective] We aimed to investigate the relationship between temperature and occurrence of Helicoverpa armigera （H0bner） in Shihezi Zong Chang. [Method] The data of occurrence of adult Helicoverpa armigera （Hubner） and temperature in 2007-2010 was analyzed for building the prediction model. [Result] The amount of overwintering generation of Helicoverpa armigera （Hubner） increased with the in- crease of temperature and its population dynamics showed a positive correlation with temperature. The amount of first and second generation of Helicoverpa armigera （Hubner） decreased with the increase of temperature and its population dynamics showed a negative correlation with temperature. [Conclusion] This research would provide a basis for the forecast and prevention of Helicoverpa armigera （Hubner） in North Xinjiang.
基金supported by the National 863 Program of China(2012AA101502)the Special Fund for Agro-Scientific Research in the Public Interest, China (200903033)
文摘Sublethal effects of chlorantraniliprole to Helicoverpa armigera （Hiibner） larvae were evaluated through exposure of third instar larvae to the insecticide incorporated into an artificial diet. When larvae were fed on the insecticide-containing diet for 7 d, the LC10, LC20, LC40, and LC50values were 3.790, 7.977, 21.577, and 33.121 μg active ingredient L^-1, respectively. Chlorantraniliprole at sublethal concentrations significantly reduced the larval body mass, emergence ratio, adult longevity and egg hatching rate in both the parental and offspring generations. The pupation and copulation rate in the parental generation and the pupal mass in the offspring also strongly decreased. Reproduction was seriously disturbed in both the parental and offspring groups even when only one of the partners was exposed to chlorantraniliprole at larval stages. However, at the lowest concentration of exposure （LC^0）, offspring fecundity was strongly reduced only when both partners were exposed. The net reproductive rate （R0）, intrinsic rate of increase （rm）, and gross reproduction rate （GRR） at LC20, LC40 concentrations were significantly lower than that of the control. Post-exposure effects also included an extended larval developmental time and increased male proportion in both generations. The doubling time （Dt） at LC20, LC40 concentrations as well as gross reproduction rate （GRR） at LC10 concentration were also significantly increased. Chlorantraniliprole might have significant effects on H. armigera population dynamics even at sublethal concentrations on both parental and offspring generations.
基金supported by the National Basic Research Program of China(2012CB114104)the National Natural Science Foundation of China(30871640,31071694)+1 种基金the National High-Tech R&D Program of China(2008AA02Z307)the International Cooperation and Exchange Foundation of NSFC-RS of China(31111130203).
文摘The full-length sequence of the odorant binding protein 5 gene,HarmOBP5,was obtained from an antennae cDNA library of cotton bollworm,Helicoverpa armigera （Hübner）.The cDNA contains a 444 bp open reading frame,encoding a protein with 147 amino acids,namely HarmOBP5.HarmOBP5 was expressed in Escherichia coli and the recombinant protein was purified by affinity chromatography.SDS-PAGE and Western blot analysis demonstrated that the purified protein can be used for further investigation of its binding characteristics.Competitive binding assays with 113 odorant chemicals indicated that HarmOBP5 has strong affinity to some special plant volatiles,including （E）-β-farnesene,ethyl butyrate,ethyl heptanoate,and acetic acid 2-methylbutyl ester.Based on three-dimensional （3D） model of AaegOBP1 from Aedes aegypti,a 3D model of HarmOBP5 was predicted.The model revealed that some key binding residues in HarmOBP5 may play important roles in odorant perception of H.armigera.This study provides clues for better understanding physiological functions of OBPs in H.armigera and other insects.
基金Project supported by the National Basic Research Program(973)of China(No.2006CB102002)the Pilot Project of Knowledge Innovation Program of Chinese Academy of Sciences(No.KSCX2-YW-N-006)National Natural Science Foundation of China(No.30571253,30621003)
文摘The growth, development and consumption of successive three generations of cotton bollworm, Helicoverpa armigera （Htibner）, fed on cotton bolls grown under elevated CO2 （double-ambient vs. ambient） in open-top chambers were examined. Significant decreases in protein, total amino acid, water and nitrogen content and increases in free fatty acid were observed in cotton bolls. Changes in quality of cotton bolls affected the growth, development and food utilization of H. armigera. Significantly longer larval development duration in three successive generations and lower pupal weight of the second and third generations were observed in cotton bollworm fed on cotton bolls grown under elevated CO2. Significantly lower fecundity was also found in successive three generations of H. armigera fed on cotton bolls grown under elevated CO2. The consumption per larva occurred significant increase in successive three generations and frass per larva were also significantly increased during the second and third generations under elevated CO2. Significantly lower relative growth rate, efficiency of conversion of ingested food and significant higher relative consumption rate in successive three generations were observed in cotton bollworm fed on cotton bolls grown under elevated CO2. Significantly lower potential female fecundity, larval numbers and population consumption were found in the second and third generations of cotton bollworm fed on cotton bolls grown under elevated CO2. The integrative effect of higher larval mortality rate and lower adult fecundity resulted in significant decreases in potential population consumption in the latter two generations. The results show that elevated CO2 adversely affects cotton bolls quality, which indicates the potential population dynamics and potential population consumption of cotton bollworm will alleviate the harm to the plants in the future rising CO2 atmosphere.
基金supported by the National 973 Program of China (2012CB114104)the National Natural Science Foundation of China (31171858)
文摘A chemosensory protein named HarmCSP5 in cotton bollworm Helicoverpa armigera （Hvbner） was obtained from antennal eDNA libraries and expressed in Escherichia coll. The real time quantitative PCR （RT-qPCR） results indicated that HarmCSP5 gene was mainly expressed in male and female antennae but also expressed in female legs and wings. Competitive binding assays were performed to test the binding affinity of recombinant HarmCSP5 to 60 odor molecules including some cotton volatiles. The resules showed that HarmCSP5 showed strong binding abilities to 4-ehtylbenzaldehyde and 3,4-dimethlbenz aldehyde, whereas methyl phenylacetate, 2-decanone, 1-pentanol, carvenol, isobomeol, nerolidol, 2- nonanone and ethyl heptanoate have relatively weak binding affinity. Moreover, the predicted 3D model of HarmCSP5 consists of six α-helices located among residues 33-38 （αl）, 40-48 （α2）, 62-72 （α3）, 80-96 （α4）, 98-108 （α5）, and 116-119 （α6）, two pairs of disulfide bridges Cys49-Cys55, Cys75-Cys78. The two amino acid residues, Ile94 and Trpl01, may play crucial roles in HarmCSP5 binding with ligands and need further study for confirmation.
文摘Electroantennogram (EAG) evaluation of selected compounds from wilted leaves of black poplar, Populus nigra, showed that phenyl acetaldehyde, methyl salicylate, (E)-2-hexenal elicited strong responses from male antennae of Helicoverpa armigera. When mixed with sex pheromone (Ph), some volatiles, e.g. phenyl acetaldehyde, benzyl alcohol, phenylethanol, methylsalicylate, linalool, benzaldehyde, (Z)-3-hexenol, (Z)-3-hexenylacetate, (Z)-6-nonenol, cineole, (E)-2-hexenal, and geraniol elicited stronger responses from male antennae than Ph alone. Wind tunnel bioassay demonstrated that various volatiles could either enhance or inhibit the effect of synthetic sex pheromone. (E)-2-hexenal, (Z)-3-hexenol and linalool in combination with Ph could not induce any male to land on source at all, whereas phenyl acetaldehyde, benzaldehyde, (Z)-6-nonenol and salicylaldehyde combined with Ph enhanced male response rates by 58.63%, 50.33%, 51.85% and 127.78%, respectively, compared to Ph alone. These results suggested that some volatiles shouldmodify sex pheromone caused behavior and that some of them could possibly be used as a tool for disrupting mating or for enhancing the effect of synthetic sex pheromone in the field.
基金supported by the National Key Research and Development Program of China(2017YFB0403905)the Central Public-interest Scientific Institution Basal Research Fund,China(Y2019YJ06)the Agricultural Science and Technology Innovation Program of China(ASTIP-TRIC04)。
文摘Light traps are widely utilized to monitor and manage insect pest populations.In late 2018,the fall armyworm(FAW),Spodoptera frugiperda,invaded China through Yunnan Province representing a huge threat to grain production.To estimate the efficiency of light traps on FAW moths,we first identified the opsin genes from FAW by using the transcriptome.Phylogenetic analysis indicated that the four opsins of FAW were clustered with those of other Noctuidae species.The expressed levels of opsins in S.frugiperda were lower than in Helicoverpa armigera,suggesting a different phototactic response between the two species.Then,we determined the phototactic behavior of FAW using H.armigera as a control,which is widely monitored and managed using light traps in China.Our results indicated that the two moths species showed significantly different phototactic behavior and both female and male FAW displayed faster flight-to-light speed than H.armigera.This may be due to a faster flight capacity in FAW compared to H.armigera.However,the capture rate of both female and male of S.frugiperda was significantly lower than that of H.armigera,which was consistent with the expression levels of opsins.These results support the positive phototaxis of S.frugiperda moths and suggest light traps could be used for monitoring and managing the pests,but with a lower efficiency than H.armigera.
基金Supported by University Grant Commission(UGC)X plan(Grant No.UGC/TN/GAC-2008)
文摘Objective:To evaluate the antifeedant,insecticidal and growth inhibition activities of Solanum pseudocapsicum(S.pseudocapsicum)seed extracts against Spodoptera litura(S.litura)and Helicotverpa armigera(H.armigera).Methods:Hcxane,diethyl cther,dichloromethanc and ethyl acetate seed extracts were prepared and tested for autifeedant,insecticidal and growth inhibitory activities against fourth instar larvae of&litura and H.armigera.Results:Ethyl acetate extract showed promising antifeedant and insecticidal activities against S.litura and H.armigera.Percentage of deformed larvae,pupae and adults were maximum in treatment of ethyl acetate extract.Percentage of successful adult emergence was deteriorated by seeds on extract treated larvae.Conclusions:Ethyl acetate extracts of S.pseudocapsicum,showed higher efficiency of antifeedant,insecticidal and growth inhibition activities.Hence,it can be used to controll agricultural insect pests,S.litura and H.armigera.
基金Research was supported by the National Natural Science Fundation of China(30200182)973 Program of Ministry of Science and Techno1ogy,China(001CB109004).
文摘The brush border membrane vesicles (BBMVs) in midgut of Helicoverpa armigera weresuccessfully separated, and most of the Aminopeptidase N (APN) activities in BBMV werepreserved. The 3-[(3-chlor-amidopropyl) dimethylammonio]-1-propane-sulphonate (CHAPS)can enhance the dissolution of BBMV, and phosphatidylinositol-specific phosopholipase C(PI-PLC) can cleave the APN from midgut membrane. The APN was primarily purified usinga Mono-Q column. The results of immunoblotting showed that the 120 and 170kDa proteinsin the BBMV could bind Cry1Ac, and 120kDa APN was a glycosylphosphalidylinositol(GPI)-anchored protein. Two Bt-resistant strains (Bt-P, Bt-M) were obtained after beingselected for more than five years in laboratory using Bt insecticides and Bt transgeniccotton incorporated into diet separately. The resistance of Bt-P and Bt-M were 1083.3and 48.7 times that of susceptible strain. The genes encoding APN1 in midgut ofsusceptible and resistant H.armigera were cloned by PCR and RACE techniques. Theinferred amino acid sequences of APN1 possessed the common character of APN family ininsects. In comparison with APN1 in susceptible strain, three nucleotide mutations wereobserved in the APN1 of Bt-M strain and resulted in two amino acid replace in theputative protein sequences, and eight nucleotide mutations were observed in Bt-P strainand resulted in five amino acid replace.
