The transcriptional cascade and regulatory loop play crucial roles in regulating plant-specialized metabolite biosynthesis.Capsaicinoids are unique to the genus Capsicum and confer a pungent flavor to its fruits.Howev...The transcriptional cascade and regulatory loop play crucial roles in regulating plant-specialized metabolite biosynthesis.Capsaicinoids are unique to the genus Capsicum and confer a pungent flavor to its fruits.However,the transcriptional regulation of capsaicinoid biosynthesis remains largely unknown.In this study,two AP2/ERF transcription factors(TFs),CaERF102 and CaERF111,were characterized for their role in the capsaicinoid biosynthesis process.Expression analysis of two ERFs and capsaicinoid biosynthetic genes(CBGs)suggested that they were associated with capsaicinoid biosynthesis.Both ERFs encode nuclear-localized proteins and function as transcriptional activators through their C-terminal activation motifs.The two ERF TFs participated in capsaicinoid biosynthesis by directly activating the promoters of key CBGs,and this activation was significantly enhanced when CaMYC2 was co-expressed.Moreover,CaERF102 and CaERF111 were found to interact with CaMYC2.This study helps elucidate the AP2/ERF TF regulatory network that governs capsaicinoid biosynthesis in Capsicum species.展开更多
印刷企业为防止订单状况多、管控疏漏等问题,提升生产效率,大多引入高级计划排程APS(Advanced Planning and Scheduling)系统,全盘考虑印刷企业内外资源与能力,实现企业生产管理的全局优化目标。本文论述并讨论了APS高级计划排程系统在...印刷企业为防止订单状况多、管控疏漏等问题,提升生产效率,大多引入高级计划排程APS(Advanced Planning and Scheduling)系统,全盘考虑印刷企业内外资源与能力,实现企业生产管理的全局优化目标。本文论述并讨论了APS高级计划排程系统在软包装印刷企业中的应用情况和发展趋势,并针对APS在软包装印刷企业中的应用,提出了基于系统集成和生产线建模的APS印刷车间场景建模方法,希望能对未来的相关研究起到借鉴作用。展开更多
基金funded by the National Natural Science Foundation of China(Grant Nos.32202502,U21A20230,32070331,32102380 and 32072580)National Key Research and Development Program(Grant No.2018YFD1000800)+3 种基金the Key-Area Research and Development Program of Guangdong Province(Grant No.2022B0202080001)the Special Fund for Seed Industry of Guangdong Province Rural Revitalization Strategy(Grant No.2022-NPY00-024)Tibet Autonomous Region of Lhasa City Science and Technology Project(Grant No.LSKJ202310)the Science and Technology Project of Bijie City(Grant No.BKK2022-3)。
文摘The transcriptional cascade and regulatory loop play crucial roles in regulating plant-specialized metabolite biosynthesis.Capsaicinoids are unique to the genus Capsicum and confer a pungent flavor to its fruits.However,the transcriptional regulation of capsaicinoid biosynthesis remains largely unknown.In this study,two AP2/ERF transcription factors(TFs),CaERF102 and CaERF111,were characterized for their role in the capsaicinoid biosynthesis process.Expression analysis of two ERFs and capsaicinoid biosynthetic genes(CBGs)suggested that they were associated with capsaicinoid biosynthesis.Both ERFs encode nuclear-localized proteins and function as transcriptional activators through their C-terminal activation motifs.The two ERF TFs participated in capsaicinoid biosynthesis by directly activating the promoters of key CBGs,and this activation was significantly enhanced when CaMYC2 was co-expressed.Moreover,CaERF102 and CaERF111 were found to interact with CaMYC2.This study helps elucidate the AP2/ERF TF regulatory network that governs capsaicinoid biosynthesis in Capsicum species.
文摘印刷企业为防止订单状况多、管控疏漏等问题,提升生产效率,大多引入高级计划排程APS(Advanced Planning and Scheduling)系统,全盘考虑印刷企业内外资源与能力,实现企业生产管理的全局优化目标。本文论述并讨论了APS高级计划排程系统在软包装印刷企业中的应用情况和发展趋势,并针对APS在软包装印刷企业中的应用,提出了基于系统集成和生产线建模的APS印刷车间场景建模方法,希望能对未来的相关研究起到借鉴作用。
