A new cyclic nonapeptide, amanexitide (1), along with the known cyclopeptides, a- and b-amanitins, was isolated from the fruiting bodies of Amanita exitialis, a newly described poisonous mushroom endemic to Guangzhou,...A new cyclic nonapeptide, amanexitide (1), along with the known cyclopeptides, a- and b-amanitins, was isolated from the fruiting bodies of Amanita exitialis, a newly described poisonous mushroom endemic to Guangzhou, Guangdong Province, China. Its amino acid composition and absolute configuration were determined by chemical degradation and derivatization followed by chiral GC analysis. Its amino acid sequence was elucidated by extensive analysis of ESIMS/MS and FTICRMS data. The occurrence of 1 in this mushroom is interesting because it has a structure closely related to antamanide, a cyclic decapeptide with antidote activity against amatoxins and phallotoxins, occurring in A. phalloides.展开更多
A new purine nucleoside coupled with an amino acid derivative, N^2-（l-methoxycarbonylethyl）guanosine 1, along with β- carboline and russulaceramide was isolated from the fruiting bodies of Amanita exitialis, a newl...A new purine nucleoside coupled with an amino acid derivative, N^2-（l-methoxycarbonylethyl）guanosine 1, along with β- carboline and russulaceramide was isolated from the fruiting bodies of Amanita exitialis, a newly described poisonous mushroom. Its structure was elucidated by spectroscopic methods. This is the first report of naturally occurring nucleosides in which an a-amino acid derivative is bonded through its a-amino nitrogen to a nucleobase aglycone by a C-N bond. The new compound was found to be toxic in brine shrimp lethality test （BST）. C 2009 Published by Elsevier B.V. on behalf of Chinese Chemical Society.展开更多
It is widely known that the genus Amanita includes various taxa: edible and inedible, dangerous and sometimes even deadly species. Despite the number of studies carried out on this genus, the toxicity of some species,...It is widely known that the genus Amanita includes various taxa: edible and inedible, dangerous and sometimes even deadly species. Despite the number of studies carried out on this genus, the toxicity of some species, such as the group of white fungi, remains dubious. Hence Amanita ovoidea (Bull.) Link. is considered as edible and even tasty both popularly and in some scientific publications, and as poisonous in others, having caused various cases of poisoning. This study stems from a case of poisoning by A. ovoidea in Siena (Italy). Tests were performed with the aim of finding a scientific answer regarding the real edibility or inedibility of A. ovoidea. Analyses included phytochemical screening, toxicological tests on human ex vivo peripheral blood mononuclear cells, and measurements of soil and mushroom pollution by heavy metals.展开更多
Objective： To assess the effects of treatment of Amanita mushroom poisoning with Glossy anoderma Decoction （灵芝煎剂, GGD）. Methods： Twelve patients with acute Amanita mushroom poisoning received conventional trea...Objective： To assess the effects of treatment of Amanita mushroom poisoning with Glossy anoderma Decoction （灵芝煎剂, GGD）. Methods： Twelve patients with acute Amanita mushroom poisoning received conventional treatment （penicillin and reduced glutathione） combined with oral administration of GGD （treated group）, which was prepared out of 200 g Glossy ganoderma decocted in water to 600 mL, and 200 ml was given once, three times a day for 7 successive days; while conventional treatment alone was given to the other 11 patients assigned to the control group. The therapeutic efficacy and changes in serum levels of total bilirubin （TBil）, bile acids （BA）, alanine transaminase （ALl-）, and aspartate transaminase （AST） activities in the two groups were compared. Results： The cured- markedly effective rate in the treated group was more significant than that in the control group （P〈0.01）. Elevation in TBil, BA, ALT, and AST activities were observed in both groups 3 days after poisoning, which progressively increased thereafter in the control group. In the treated group, they reached their peak on the 3rd day and then declined gradually. The differences between pre-treatment and post-treatment in both groups were obviously significant （P〈0.01）, so were the differences between the two groups at corresponding time points （P〈0.01）. Conclusion： GGD shows excellent clinical efficacy in the treatment of acute Amanita mushroom poisoning and can reduce mortality significantly.展开更多
基金This work was supported by the Knowledge Innovation Program of the Chinese Academy of Sciences(No.KSCX2-YW-N-036).
文摘A new cyclic nonapeptide, amanexitide (1), along with the known cyclopeptides, a- and b-amanitins, was isolated from the fruiting bodies of Amanita exitialis, a newly described poisonous mushroom endemic to Guangzhou, Guangdong Province, China. Its amino acid composition and absolute configuration were determined by chemical degradation and derivatization followed by chiral GC analysis. Its amino acid sequence was elucidated by extensive analysis of ESIMS/MS and FTICRMS data. The occurrence of 1 in this mushroom is interesting because it has a structure closely related to antamanide, a cyclic decapeptide with antidote activity against amatoxins and phallotoxins, occurring in A. phalloides.
基金supported by the Knowledge Innovation Program of the Chinese Academy of Sciences(No.KSCX2-YW-N-036).
文摘A new purine nucleoside coupled with an amino acid derivative, N^2-（l-methoxycarbonylethyl）guanosine 1, along with β- carboline and russulaceramide was isolated from the fruiting bodies of Amanita exitialis, a newly described poisonous mushroom. Its structure was elucidated by spectroscopic methods. This is the first report of naturally occurring nucleosides in which an a-amino acid derivative is bonded through its a-amino nitrogen to a nucleobase aglycone by a C-N bond. The new compound was found to be toxic in brine shrimp lethality test （BST）. C 2009 Published by Elsevier B.V. on behalf of Chinese Chemical Society.
文摘It is widely known that the genus Amanita includes various taxa: edible and inedible, dangerous and sometimes even deadly species. Despite the number of studies carried out on this genus, the toxicity of some species, such as the group of white fungi, remains dubious. Hence Amanita ovoidea (Bull.) Link. is considered as edible and even tasty both popularly and in some scientific publications, and as poisonous in others, having caused various cases of poisoning. This study stems from a case of poisoning by A. ovoidea in Siena (Italy). Tests were performed with the aim of finding a scientific answer regarding the real edibility or inedibility of A. ovoidea. Analyses included phytochemical screening, toxicological tests on human ex vivo peripheral blood mononuclear cells, and measurements of soil and mushroom pollution by heavy metals.
基金Supported by the Funds from Hunan Provincial Administration of Traditional Chinese Medicine (No 204054)
文摘Objective： To assess the effects of treatment of Amanita mushroom poisoning with Glossy anoderma Decoction （灵芝煎剂, GGD）. Methods： Twelve patients with acute Amanita mushroom poisoning received conventional treatment （penicillin and reduced glutathione） combined with oral administration of GGD （treated group）, which was prepared out of 200 g Glossy ganoderma decocted in water to 600 mL, and 200 ml was given once, three times a day for 7 successive days; while conventional treatment alone was given to the other 11 patients assigned to the control group. The therapeutic efficacy and changes in serum levels of total bilirubin （TBil）, bile acids （BA）, alanine transaminase （ALl-）, and aspartate transaminase （AST） activities in the two groups were compared. Results： The cured- markedly effective rate in the treated group was more significant than that in the control group （P〈0.01）. Elevation in TBil, BA, ALT, and AST activities were observed in both groups 3 days after poisoning, which progressively increased thereafter in the control group. In the treated group, they reached their peak on the 3rd day and then declined gradually. The differences between pre-treatment and post-treatment in both groups were obviously significant （P〈0.01）, so were the differences between the two groups at corresponding time points （P〈0.01）. Conclusion： GGD shows excellent clinical efficacy in the treatment of acute Amanita mushroom poisoning and can reduce mortality significantly.
